Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_005_M01
(849 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P42932|TCPQ_MOUSE T-complex protein 1, theta subunit (TC... 285 1e-76
sp|P50990|TCPQ_HUMAN T-complex protein 1, theta subunit (TC... 283 4e-76
sp|P47079|TCPQ_YEAST T-complex protein 1, theta subunit (TC... 241 1e-63
sp|P78921|TCPQ_SCHPO Probable T-complex protein 1, theta su... 241 2e-63
sp|P47828|TCPQ_CANAL T-complex protein 1, theta subunit (TC... 173 6e-43
sp|O24735|THSB_SULTO Thermosome beta subunit (Thermosome su... 114 3e-25
sp|Q58405|THS_METJA Thermosome subunit (Chaperonin subunit) 112 1e-24
sp|P28488|THSB_SULSH Thermosome beta subunit (Thermosome su... 110 5e-24
sp|Q9V2T4|THSB_SULAC Thermosome beta subunit (Thermosome su... 110 5e-24
sp|O24731|THSA_THEK8 Thermosome alpha subunit (Thermosome s... 109 8e-24
>sp|P42932|TCPQ_MOUSE T-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
Length = 548
Score = 285 bits (729), Expect = 1e-76
Identities = 140/270 (51%), Positives = 200/270 (74%)
Frame = +3
Query: 39 LLKMGLSVPDVTEGFEIAAKHSLEILKDLVIGKVEDLREKEMIKKIVGTAVNSKIFGEES 218
LL++GLSV +V G+EIA K + EIL +LV ++LR+ + + ++ T++ SK +G E+
Sbjct: 118 LLRIGLSVSEVISGYEIACKKAHEILPELVCCSAKNLRDVDEVSSLLRTSIMSKQYGSET 177
Query: 219 HISDLVVDACIRALPTSSFFNVDNIRVVKILGSGTNASKVFNGMVFVREAQSVIKECKAA 398
++ L+ AC+ P S FNVDNIRV KILGSG +S V +GMVF +E + + K A
Sbjct: 178 FLAKLIAQACVSIFPDSGNFNVDNIRVCKILGSGIYSSSVLHGMVFKKETEGDVTSVKDA 237
Query: 399 KVVVFSCPFDQLQTETKGTVLINTAKELMDFSRGEEDQMEERVREIAETGCKVIVTGGKV 578
K+ V+SCPFD + TETKGTVLI TA+ELM+FS+GEE+ M+ +V+ IA TG VIVTGGKV
Sbjct: 238 KIAVYSCPFDGMITETKGTVLIKTAEELMNFSKGEENLMDAQVKAIAGTGANVIVTGGKV 297
Query: 579 GELALHYANKYNLMVVKLSSKFDIRRVCKVTGATPIPKLSTPTQEELGYIASVRTEEIGK 758
++ALHYANKYN+M+V+L+SK+D+RR+CK GAT +PKL+ P QEE+G+ SV E+G
Sbjct: 298 ADIALHYANKYNIMLVRLNSKWDLRRLCKTVGATALPKLTPPVQEEMGHCDSVYLSEVGD 357
Query: 759 SAVVLFDQDVKEGAISSIVVRGSMYDVMDE 848
+ VV+F + ++GAIS+IV+RGS ++MD+
Sbjct: 358 TQVVVFKHEKEDGAISTIVLRGSTDNLMDD 387
>sp|P50990|TCPQ_HUMAN T-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
sp|Q5RAP1|TCPQ_PONPY T-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
Length = 548
Score = 283 bits (724), Expect = 4e-76
Identities = 137/270 (50%), Positives = 201/270 (74%)
Frame = +3
Query: 39 LLKMGLSVPDVTEGFEIAAKHSLEILKDLVIGKVEDLREKEMIKKIVGTAVNSKIFGEES 218
LL++GLSV +V EG+EIA + + EIL +LV ++LR+ + + ++ T++ SK +G E
Sbjct: 118 LLRIGLSVSEVIEGYEIACRKAHEILPNLVCCSAKNLRDIDEVSSLLRTSIMSKQYGNEV 177
Query: 219 HISDLVVDACIRALPTSSFFNVDNIRVVKILGSGTNASKVFNGMVFVREAQSVIKECKAA 398
++ L+ AC+ P S FNVDNIRV KILGSG ++S V +GMVF +E + + K A
Sbjct: 178 FLAKLIAQACVSIFPDSGHFNVDNIRVCKILGSGISSSSVLHGMVFKKETEGDVTSVKDA 237
Query: 399 KVVVFSCPFDQLQTETKGTVLINTAKELMDFSRGEEDQMEERVREIAETGCKVIVTGGKV 578
K+ V+SCPFD + TETKGTVLI TA+ELM+FS+GEE+ M+ +V+ IA+TG V+VTGGKV
Sbjct: 238 KIAVYSCPFDGMITETKGTVLIKTAEELMNFSKGEENLMDAQVKAIADTGANVVVTGGKV 297
Query: 579 GELALHYANKYNLMVVKLSSKFDIRRVCKVTGATPIPKLSTPTQEELGYIASVRTEEIGK 758
++ALHYANKYN+M+V+L+SK+D+RR+CK GAT +P+L+ P EE+G+ SV E+G
Sbjct: 298 ADMALHYANKYNIMLVRLNSKWDLRRLCKTVGATALPRLTPPVLEEMGHCDSVYLSEVGD 357
Query: 759 SAVVLFDQDVKEGAISSIVVRGSMYDVMDE 848
+ VV+F + ++GAIS+IV+RGS ++MD+
Sbjct: 358 TQVVVFKHEKEDGAISTIVLRGSTDNLMDD 387
>sp|P47079|TCPQ_YEAST T-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
Length = 568
Score = 241 bits (616), Expect = 1e-63
Identities = 123/280 (43%), Positives = 189/280 (67%), Gaps = 10/280 (3%)
Frame = +3
Query: 39 LLKMGLSVPDVTEGFEIAAKHSLEILKDLVIGKVEDLREKEMIKKIVGTAVNSKIFGEES 218
L+ MGLS ++ +G+ +A K +L+ L ++V+G++ D +K + K++ ++SK +G E
Sbjct: 117 LISMGLSAVEIIQGYNMARKFTLKELDEMVVGEITDKNDKNELLKMIKPVISSKKYGSED 176
Query: 219 HISDLVVDACIRALPTSS------FFNVDNIRVVKILGSGTNASKVFNGMVFVREAQSVI 380
+S+LV +A LP + +FNVD+IRVVKI+G + S V GMVF RE + +
Sbjct: 177 ILSELVSEAVSHVLPVAQQAGEIPYFNVDSIRVVKIMGGSLSNSTVIKGMVFNREPEGHV 236
Query: 381 K---ECKAAKVVVFSCPFDQLQTETKGTVLINTAKELMDFSRGEEDQMEERVREIAETGC 551
K E K KV VF+CP D TETKGTVL++ A+E++DFS+GEE Q++ ++EIA+ G
Sbjct: 237 KSLSEDKKHKVAVFTCPLDIANTETKGTVLLHNAQEMLDFSKGEEKQIDAMMKEIADMGV 296
Query: 552 KVIVTGGKVGELALHYANKYNLMVVKLSSKFDIRRVCKVTGATPIPKLSTPTQEELGYIA 731
+ IV G VGELALHY N+Y ++V+K+ SKF++RR+C+V GATP+P+L PT EELG +
Sbjct: 297 ECIVAGAGVGELALHYLNRYGILVLKVPSKFELRRLCRVCGATPLPRLGAPTPEELGLVE 356
Query: 732 SVRTEEIGKSAVVLFDQDVKE-GAISSIVVRGSMYDVMDE 848
+V+T EIG V +F Q+ E S+I++RG+ + +D+
Sbjct: 357 TVKTMEIGGDRVTVFKQEQGEISRTSTIILRGATQNNLDD 396
>sp|P78921|TCPQ_SCHPO Probable T-complex protein 1, theta subunit (TCP-1-theta)
(CCT-theta)
Length = 546
Score = 241 bits (615), Expect = 2e-63
Identities = 116/271 (42%), Positives = 183/271 (67%), Gaps = 1/271 (0%)
Frame = +3
Query: 39 LLKMGLSVPDVTEGFEIAAKHSLEILKDLVIGKVEDLREKEMIKKIVGTAVNSKIFGEES 218
+++MGL+ ++ +G+E+A H++E+L+++ K+E + ++ + K + T ++SK +G E
Sbjct: 117 MIRMGLTPLEIAKGYEMALSHTMEVLEEICADKIETVESEKELIKAIRTCISSKQYGNED 176
Query: 219 HISDLVVDACIRALPTS-SFFNVDNIRVVKILGSGTNASKVFNGMVFVREAQSVIKECKA 395
+SDLV A + LP S FNVDNIRVVKI+GS S+V GMVF RE + + K
Sbjct: 177 FLSDLVAKAILTVLPKDPSKFNVDNIRVVKIMGSSLYNSQVVKGMVFprePEGTVTRSKE 236
Query: 396 AKVVVFSCPFDQLQTETKGTVLINTAKELMDFSRGEEDQMEERVREIAETGCKVIVTGGK 575
AKV VFSCP D QTETKGTVL++ A+E++DFS+GEE+ +E ++EI + G +V+VT G
Sbjct: 237 AKVAVFSCPLDISQTETKGTVLLHNAQEMLDFSKGEENLIESHIKEIYDAGVRVVVTSGN 296
Query: 576 VGELALHYANKYNLMVVKLSSKFDIRRVCKVTGATPIPKLSTPTQEELGYIASVRTEEIG 755
V +L LHY N++ ++V+++ SKF++RR+C+V GATP+ ++ P EE+G + V T EIG
Sbjct: 297 VNDLVLHYLNRFEILVIRVPSKFELRRLCRVVGATPLARMGVPMPEEMGSVDVVETIEIG 356
Query: 756 KSAVVLFDQDVKEGAISSIVVRGSMYDVMDE 848
V +F Q ++IV+RG+ +D+
Sbjct: 357 GDRVTVFRQVEDITRTATIVLRGATKTYLDD 387
>sp|P47828|TCPQ_CANAL T-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
Length = 540
Score = 173 bits (438), Expect = 6e-43
Identities = 99/271 (36%), Positives = 157/271 (57%), Gaps = 1/271 (0%)
Frame = +3
Query: 39 LLKMGLSVPDVTEGFEIAAKHSLEILKDLVIGKVEDLREKEMIKKIVGTAVNSKIFGEES 218
LL +GL+V ++ +GF +A K ++ L +LV+ KVE E +++K V + +K +G E
Sbjct: 117 LLTLGLNVSEIIQGFNLANKFVMKTLDELVVEKVESF-ETDLLKA-VKPVIAAKQYGVED 174
Query: 219 HISDLVVDACIRALPTSSFFNVDNIRVVKILGSGTNASKVFNGMVFVREAQSVIKECKAA 398
I+ LVVDA + SF NVDNIRVVK++G+ + S+V GMVF RE + +K
Sbjct: 175 TIAKLVVDAVALVMKNGSF-NVDNIRVVKVMGASLSQSQVVKGMVFprePEGTVKNADQI 233
Query: 399 KVVVFSCPFDQLQTETKGTVLINTA-KELMDFSRGEEDQMEERVREIAETGCKVIVTGGK 575
+ P + +T ++ + R + + R+ G + ++ G
Sbjct: 234 QSCRVYQPHRYFHHRNQRYSCSSTMPRKCLISPRAKNNSWTSCARKSTIQGLRWLLPGSS 293
Query: 576 VGELALHYANKYNLMVVKLSSKFDIRRVCKVTGATPIPKLSTPTQEELGYIASVRTEEIG 755
VGELALHY NKY ++V+++ SKFD+RR+C+V GATP+P+L PT +E+G I + T+EIG
Sbjct: 294 VGELALHYLNKYGILVLRVPSKFDLRRICQVCGATPLPRLGAPTPDEMGEIDIIETKEIG 353
Query: 756 KSAVVLFDQDVKEGAISSIVVRGSMYDVMDE 848
V +F QD ++IVVRG+ + +D+
Sbjct: 354 GDRVTIFRQDESSSRTATIVVRGATQNNLDD 384
>sp|O24735|THSB_SULTO Thermosome beta subunit (Thermosome subunit 2) (Chaperonin beta
subunit)
Length = 552
Score = 114 bits (285), Expect = 3e-25
Identities = 68/266 (25%), Positives = 140/266 (52%), Gaps = 6/266 (2%)
Frame = +3
Query: 69 VTEGFEIAAKHSLEILKDLVIGKVEDLREKEMIKKIVGTAVNSK-IFGEESHISDLVVDA 245
+ GF+ A + +L+ +++ I + + + +++KK+ T++NSK + G +++D+V A
Sbjct: 131 IVSGFKKAEEQALKTIEE--IAQKVSVNDMDILKKVAMTSLNSKAVAGAREYLADIVAKA 188
Query: 246 CIRALPTSS---FFNVDNIRVVKILGSGTNASKVFNGMVFVREA--QSVIKECKAAKVVV 410
+ + ++DNI++VK G N +++ G+V +E + K + AK+ +
Sbjct: 189 VTQVAELRGDRWYVDLDNIQIVKKHGGSINDTQIIYGIVVDKEVVHPGMPKRVENAKIAL 248
Query: 411 FSCPFDQLQTETKGTVLINTAKELMDFSRGEEDQMEERVREIAETGCKVIVTGGKVGELA 590
+ + E + IN ++ F EE+ ++E+V +IA TG V++ + E+A
Sbjct: 249 LDASLEVEKPELDAEIRINDPTQMKKFLEEEENLLKEKVDKIAATGANVVICQKGIDEVA 308
Query: 591 LHYANKYNLMVVKLSSKFDIRRVCKVTGATPIPKLSTPTQEELGYIASVRTEEIGKSAVV 770
HY K ++ V+ + K D+ ++ + TG + + T ++LGY A V ++G+ +V
Sbjct: 309 QHYLAKKGILAVRRAKKSDLEKLARATGGRVVSNIDELTPQDLGYAALVEERKVGEDKMV 368
Query: 771 LFDQDVKEGAISSIVVRGSMYDVMDE 848
F + K SI++RG + V+DE
Sbjct: 369 -FVEGAKNPKAVSILIRGGLERVVDE 393
>sp|Q58405|THS_METJA Thermosome subunit (Chaperonin subunit)
Length = 542
Score = 112 bits (281), Expect = 1e-24
Identities = 81/277 (29%), Positives = 141/277 (50%), Gaps = 7/277 (2%)
Frame = +3
Query: 39 LLKMGLSVPDVTEGFEIAAKHSLEILKDLVIGKVEDLREKEMIKKIVGTAVNSKIFGEES 218
LL + + G+E+A ++E LK I K + EM+KKI T++ K G E
Sbjct: 113 LLDQNIHPSVIINGYEMARNKAVEELKS--IAKEVKPEDTEMLKKIAMTSITGK--GAEK 168
Query: 219 ---HISDLVVDACIRALPTSSFFNVDN--IRVVKILGSGTNASKVFNGMVFVREAQS--V 377
++++VV+A +RA+ VD I+V K G+ +K+ G+V +E + +
Sbjct: 169 AREQLAEIVVEA-VRAVVDEETGKVDKDLIKVEKKEGAPIEETKLIRGVVIDKERVNPQM 227
Query: 378 IKECKAAKVVVFSCPFDQLQTETKGTVLINTAKELMDFSRGEEDQMEERVREIAETGCKV 557
K+ + AK+ + +CP + +TET + I +LM+F EE +++ V +IA TG V
Sbjct: 228 PKKVENAKIALLNCPIEVKETETDAEIRITDPAKLMEFIEQEEKMIKDMVEKIAATGANV 287
Query: 558 IVTGGKVGELALHYANKYNLMVVKLSSKFDIRRVCKVTGATPIPKLSTPTQEELGYIASV 737
+ + +LA HY K ++ V+ K D+ ++ K TGA + K+ T E+LG V
Sbjct: 288 VFCQKGIDDLAQHYLAKKGILAVRRVKKSDMEKLAKATGARIVTKIDDLTPEDLGEAGLV 347
Query: 738 RTEEIGKSAVVLFDQDVKEGAISSIVVRGSMYDVMDE 848
++ A++ +Q K +I+ RGS V++E
Sbjct: 348 EERKVAGDAMIFVEQ-CKHPKAVTILARGSTEHVVEE 383
>sp|P28488|THSB_SULSH Thermosome beta subunit (Thermosome subunit 2) (Chaperonin beta
subunit) (Thermophilic factor 55 beta) (TF55-beta) (Ring
complex beta subunit)
Length = 552
Score = 110 bits (275), Expect = 5e-24
Identities = 68/266 (25%), Positives = 140/266 (52%), Gaps = 6/266 (2%)
Frame = +3
Query: 69 VTEGFEIAAKHSLEILKDLVIGKVEDLREKEMIKKIVGTAVNSK-IFGEESHISDLVVDA 245
+ G++ A + +L+ ++D I + + + ++++K+ T++ SK + G +++DLVV A
Sbjct: 130 IVSGYKKAEEIALKTIQD--IAQPVSINDTDVLRKVALTSLGSKAVAGAREYLADLVVKA 187
Query: 246 CIRALPTSS---FFNVDNIRVVKILGSGTNASKVFNGMVFVREA--QSVIKECKAAKVVV 410
+ + ++DN+++VK G N +++ G+V +E + K + AK+ +
Sbjct: 188 VAQVAELRGDKWYVDLDNVQIVKKHGGSINDTQLVYGIVVDKEVVHPGMPKRIENAKIAL 247
Query: 411 FSCPFDQLQTETKGTVLINTAKELMDFSRGEEDQMEERVREIAETGCKVIVTGGKVGELA 590
+ + E + IN ++ F EE+ ++E+V +IA TG V++ + E+A
Sbjct: 248 LDASLEVEKPELDAEIRINDPTQMHKFLEEEENILKEKVDKIAATGANVVICQKGIDEVA 307
Query: 591 LHYANKYNLMVVKLSSKFDIRRVCKVTGATPIPKLSTPTQEELGYIASVRTEEIGKSAVV 770
HY K ++ V+ + K D+ ++ + TG I + T ++LGY A V ++G+ +V
Sbjct: 308 QHYLAKKGILAVRRAKKSDLEKLARATGGRVISNIDELTSQDLGYAALVEERKVGEDKMV 367
Query: 771 LFDQDVKEGAISSIVVRGSMYDVMDE 848
F + K SI++RG + V+DE
Sbjct: 368 -FVEGAKNPKSVSILIRGGLERVVDE 392
>sp|Q9V2T4|THSB_SULAC Thermosome beta subunit (Thermosome subunit 2) (Chaperonin beta
subunit) (Thermophilic factor 55 beta) (TF55-beta)
Length = 553
Score = 110 bits (275), Expect = 5e-24
Identities = 65/266 (24%), Positives = 140/266 (52%), Gaps = 6/266 (2%)
Frame = +3
Query: 69 VTEGFEIAAKHSLEILKDLVIGKVEDLREKEMIKKIVGTAVNSK-IFGEESHISDLVVDA 245
+ G++ A + +++ +++ I + + E+++K+ T+++SK + G H++D+VV A
Sbjct: 131 IVSGYKKAEEMAIKTIEE--ISTKVSVNDTEILRKVALTSLSSKAVAGAREHLADIVVKA 188
Query: 246 CIRALPTSS---FFNVDNIRVVKILGSGTNASKVFNGMVFVREA--QSVIKECKAAKVVV 410
+ + ++DN+++VK G N +++ G++ +E + K + AK+ +
Sbjct: 189 ITQVAELRGDKWYVDLDNVQIVKKHGGSINDTQIVYGIIVDKEVVHPGMPKRVENAKIAL 248
Query: 411 FSCPFDQLQTETKGTVLINTAKELMDFSRGEEDQMEERVREIAETGCKVIVTGGKVGELA 590
+ + E + IN ++ F EE+ ++E+V +IA+TG V++ + E+A
Sbjct: 249 LDASLEVEKPELDAEIRINDPTQMKKFLDEEENILKEKVDKIAQTGANVVICQKGIDEVA 308
Query: 591 LHYANKYNLMVVKLSSKFDIRRVCKVTGATPIPKLSTPTQEELGYIASVRTEEIGKSAVV 770
HY K ++ V+ + K D+ ++ + TG + + T ++LGY V +IG+ +V
Sbjct: 309 QHYLAKKGILAVRRAKKSDLEKLARATGGRVVSNIDELTSQDLGYATLVEERKIGEDKMV 368
Query: 771 LFDQDVKEGAISSIVVRGSMYDVMDE 848
F + K SI++RG + V+DE
Sbjct: 369 -FIEGAKNPKAVSILIRGGLERVVDE 393
>sp|O24731|THSA_THEK8 Thermosome alpha subunit (Thermosome subunit 1) (Chaperonin alpha
subunit)
Length = 549
Score = 109 bits (273), Expect = 8e-24
Identities = 77/278 (27%), Positives = 137/278 (49%), Gaps = 8/278 (2%)
Frame = +3
Query: 39 LLKMGLSVPDVTEGFEIAAKHSLEILKDLVIGKVEDLREKEMIKKIVGTAVNSKIFGEES 218
LL + + +G+ +AA+ + EIL+++ I D ++E + +I T++ K ES
Sbjct: 114 LLDQNIHPSIIIKGYALAAEKAQEILEEIAIKVNPD--DEETLLRIAMTSITGK--NAES 169
Query: 219 H---ISDLVVDACIRALPTSS---FFNVDNIRVVKILGSGTNASKVFNGMVFVREA--QS 374
H ++ L VDA + ++DNI+ K G G S++ G+V +E
Sbjct: 170 HKELLAKLAVDAVKQVAEKKDGKYVVDLDNIKFEKKAGEGVEESELVRGVVIDKEVVHPR 229
Query: 375 VIKECKAAKVVVFSCPFDQLQTETKGTVLINTAKELMDFSRGEEDQMEERVREIAETGCK 554
+ K + AK+ + + + +TET + I + +LM F EE +++ V IA+TG
Sbjct: 230 MPKRVEGAKIALINEALEVKKTETDAKINITSPDQLMSFLEQEEKMLKDMVDHIAQTGAN 289
Query: 555 VIVTGGKVGELALHYANKYNLMVVKLSSKFDIRRVCKVTGATPIPKLSTPTQEELGYIAS 734
V+ + +LA HY KY +M V+ K D+ ++ K TGA + + T E+LGY
Sbjct: 290 VVFVQKGIDDLAQHYLAKYGIMAVRRVKKSDMEKLAKATGAKIVTNVKDLTPEDLGYAEI 349
Query: 735 VRTEEIGKSAVVLFDQDVKEGAISSIVVRGSMYDVMDE 848
V ++ ++ F + K +I++RG V+DE
Sbjct: 350 VEERKLAGENMI-FVEGCKNPKAVTILIRGGTEHVIDE 386
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 89,280,424
Number of Sequences: 369166
Number of extensions: 1681171
Number of successful extensions: 5337
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5043
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5279
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8341863645
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)