Planarian EST Database


Dr_sW_005_L13

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_005_L13
         (713 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q86Y39|N5BM_HUMAN  NADH-ubiquinone oxidoreductase subunit...    40   0.006
sp|P22170|TGB2_FXMV  TGB2 protein (Triple gene block 2 prote...    37   0.068
sp|Q80W89|N5BM_RAT  NADH-ubiquinone oxidoreductase subunit B...    37   0.068
sp|P46358|RFAY_XANCP  Probable RNA polymerase sigma factor rfaY    34   0.34 
sp|Q9D8B4|N5BM_MOUSE  NADH-ubiquinone oxidoreductase subunit...    34   0.34 
sp|Q8HXG6|N5BM_BOVIN  NADH-ubiquinone oxidoreductase subunit...    34   0.44 
sp|P30304|MPIP1_HUMAN  M-phase inducer phosphatase 1 (Dual s...    31   3.7  
sp|Q89A23|Y532_BUCBP  Hypothetical transport protein bbp532        31   3.7  
sp|P21906|GLF_ZYMMO  Glucose facilitated diffusion protein         30   4.9  
>sp|Q86Y39|N5BM_HUMAN NADH-ubiquinone oxidoreductase subunit B14.7 (Complex I-B14.7)
           (CI-B14.7)
          Length = 141

 Score = 40.0 bits (92), Expect = 0.006
 Identities = 26/75 (34%), Positives = 35/75 (46%), Gaps = 1/75 (1%)
 Frame = +3

Query: 267 VGSVTGYMLTGAMGGALYTMTATSSSFVRGNKDTPLNHFLAGTITSLFMLKPPYIYR-KV 443
           V  V  Y  T A  GA++ +T   S+ VR   D PLN+FL G    L +    + Y    
Sbjct: 53  VAKVGQYTFTAAAVGAVFGLTTCISAHVREKPDDPLNYFLGGCAGGLTLGARTHNYGIGA 112

Query: 444 VLSISIGIVCSIVKM 488
              +  GI  S+VKM
Sbjct: 113 AACVYFGIAASLVKM 127
>sp|P22170|TGB2_FXMV TGB2 protein (Triple gene block 2 protein) (TGBp2) (12 kDa protein)
           (ORF 3 protein)
          Length = 105

 Score = 36.6 bits (83), Expect = 0.068
 Identities = 28/90 (31%), Positives = 45/90 (50%), Gaps = 10/90 (11%)
 Frame = +3

Query: 387 AGTITSLFMLKPPYIYRKVVLSISIGIVCSIV-KMLTMNDCTRVMPNNNNL-----FIES 548
           A  I++   L+PP    K +L+++IGI  S+V  MLT N+   V  N ++L     +I+ 
Sbjct: 9   APAISTPLTLRPPPDNTKAILTVAIGIAASLVFFMLTRNNLPHVGDNIHSLPHGGSYIDG 68

Query: 549 ----GYRKPMAHYALKNMLGIPSYDMKPDY 626
                YR P + Y   N+L +  +   P Y
Sbjct: 69  TKSINYRPPASRYPSSNLL-VSLHQYSPQY 97
>sp|Q80W89|N5BM_RAT NADH-ubiquinone oxidoreductase subunit B14.7 (Complex I-B14.7)
           (CI-B14.7)
          Length = 141

 Score = 36.6 bits (83), Expect = 0.068
 Identities = 32/112 (28%), Positives = 49/112 (43%), Gaps = 5/112 (4%)
 Frame = +3

Query: 168 SKMRRGAMIGFALNSLNAF--SILSVAENLTPSIL--VGSVTGYMLTGAMGGALYTMTAT 335
           ++  R A I  AL  L     S  S+  N   S L  V  V  +  T A  GA++ +T  
Sbjct: 16  TQCHRKAYITTALGGLCGLISSAYSITHNPADSPLEAVARVGRFTFTAAAIGAMFGLTTC 75

Query: 336 SSSFVRGNKDTPLNHFLAGTITSLFMLKPPYIYRKVVLS-ISIGIVCSIVKM 488
            S+ VR   D PLN+F+ G    L +    + Y    +  + +G   ++ KM
Sbjct: 76  VSAQVREKPDDPLNYFIGGCAGGLTLGARTHSYGTAAIGCVYMGTAAALFKM 127
>sp|P46358|RFAY_XANCP Probable RNA polymerase sigma factor rfaY
          Length = 400

 Score = 34.3 bits (77), Expect = 0.34
 Identities = 30/109 (27%), Positives = 46/109 (42%), Gaps = 5/109 (4%)
 Frame = +3

Query: 162 GFSKMRRGAMIGFALNSLNAFSILSVAENLTPSILVGSVTGYMLTGAMGGAL---YTMTA 332
           G  K+    + G AL S +A   LSV   +  +I + ++TG  LT  M GA    +  TA
Sbjct: 231 GVGKLGASGLSGSALTSGSAAGALSVLLGMPMAIALLAITGVTLTTYMSGAYLLRFATTA 290

Query: 333 TSSSFVRG--NKDTPLNHFLAGTITSLFMLKPPYIYRKVVLSISIGIVC 473
             ++ +RG     T       G    L     P      VL++ + +VC
Sbjct: 291 REAAAIRGFTRLSTLTAALTCGAPLLLRAFGAPKWLALCVLAVGMSVVC 339
>sp|Q9D8B4|N5BM_MOUSE NADH-ubiquinone oxidoreductase subunit B14.7 (Complex I-B14.7)
           (CI-B14.7)
          Length = 141

 Score = 34.3 bits (77), Expect = 0.34
 Identities = 21/75 (28%), Positives = 35/75 (46%), Gaps = 1/75 (1%)
 Frame = +3

Query: 267 VGSVTGYMLTGAMGGALYTMTATSSSFVRGNKDTPLNHFLAGTITSLFMLKPPYIYRKVV 446
           V  V  Y  T A  GA++ +T   S+ VR   D PLN+F+ G    L +    + Y    
Sbjct: 53  VARVGRYTFTAAAIGAMFGLTTCVSAQVREKPDDPLNYFIGGCAGGLTLGARTHSYGTAA 112

Query: 447 LS-ISIGIVCSIVKM 488
           +  + +G   ++ K+
Sbjct: 113 MGCVYMGTAAALFKI 127
>sp|Q8HXG6|N5BM_BOVIN NADH-ubiquinone oxidoreductase subunit B14.7 (Complex I-B14.7)
           (CI-B14.7)
          Length = 141

 Score = 33.9 bits (76), Expect = 0.44
 Identities = 29/91 (31%), Positives = 43/91 (47%), Gaps = 3/91 (3%)
 Frame = +3

Query: 225 SILSVAENLTPSILVGSV-TG-YMLTGAMGGALYTMTATSSSFVRGNKDTPLNHFLAGTI 398
           S  SVA     S L G   TG Y  T A  GA++ +T+  S+ VR   D PLN+ + G  
Sbjct: 37  SAYSVALKTPTSFLEGVARTGRYTFTAAAIGAIFGLTSCISAQVREKPDDPLNYLIGGCA 96

Query: 399 TSLFMLKPPYIYRKVVLSIS-IGIVCSIVKM 488
             L +      Y     + + +G+  ++VKM
Sbjct: 97  GGLILGARTRSYGIGAAACAYMGLTAALVKM 127
>sp|P30304|MPIP1_HUMAN M-phase inducer phosphatase 1 (Dual specificity phosphatase Cdc25A)
          Length = 524

 Score = 30.8 bits (68), Expect = 3.7
 Identities = 11/34 (32%), Positives = 19/34 (55%)
 Frame = +2

Query: 425 IYL*KSGFKHFYWNCLFYCEDADYERLYSCDAQQ 526
           +Y+ K G+K F+  C  YCE   Y  ++  D ++
Sbjct: 463 LYVLKGGYKEFFMKCQSYCEPPSYRPMHHEDFKE 496
>sp|Q89A23|Y532_BUCBP Hypothetical transport protein bbp532
          Length = 403

 Score = 30.8 bits (68), Expect = 3.7
 Identities = 23/81 (28%), Positives = 40/81 (49%), Gaps = 1/81 (1%)
 Frame = +3

Query: 186 AMIGFALNSLNAFSILSVAENLTPSILVGSVTGYMLTGAMGGAL-YTMTATSSSFVRGNK 362
           A+ G AL+ + A ++  ++E + PS+L  S+  Y+    +GG L   +++  S +   N 
Sbjct: 118 ALTGLALSGVAAVAMTYLSEEMHPSVLSFSIGLYISGNTIGGFLGRFLSSLFSEYFSWNI 177

Query: 363 DTPLNHFLAGTITSLFMLKPP 425
                 FLA T   LF+   P
Sbjct: 178 ALEFISFLAFTSAVLFVYLLP 198
>sp|P21906|GLF_ZYMMO Glucose facilitated diffusion protein
          Length = 473

 Score = 30.4 bits (67), Expect = 4.9
 Identities = 24/92 (26%), Positives = 45/92 (48%), Gaps = 3/92 (3%)
 Frame = +3

Query: 213 LNAFSILSVAENLTPSILVGSVTGYMLTGAMG---GALYTMTATSSSFVRGNKDTPLNHF 383
           L+A +  S++  +  ++LVG VTG +L+G +G   G    +  +S  FV       L   
Sbjct: 48  LSATAAASLSGMVVVAVLVGCVTGSLLSGWIGIRFGRRGGLLMSSICFVAAGFGAALTEK 107

Query: 384 LAGTITSLFMLKPPYIYRKVVLSISIGIVCSI 479
           L GT  S   +   + + + +  + IG+V ++
Sbjct: 108 LFGTGGSALQI---FCFFRFLAGLGIGVVSTL 136
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 69,192,277
Number of Sequences: 369166
Number of extensions: 1306700
Number of successful extensions: 3263
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 3168
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3258
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6316483550
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)