Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_005_K13
(490 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O13811|PDI2_SCHPO Putative protein disulfide-isomerase C... 39 0.006
sp|P54399|PDI_DROME Protein disulfide-isomerase precursor (... 37 0.025
sp|P09102|PDIA1_CHICK Protein disulfide-isomerase precursor... 37 0.032
sp|P38661|PDIA6_MEDSA Probable protein disulfide-isomerase ... 35 0.072
sp|P07237|PDIA1_HUMAN Protein disulfide-isomerase precursor... 34 0.16
sp|P04785|PDIA1_RAT Protein disulfide-isomerase precursor (... 34 0.16
sp|Q5R5B6|PDIA1_PONPY Protein disulfide-isomerase precursor... 34 0.16
sp|P12244|GSBP_CHICK Dolichyl-diphosphooligosaccharide-prot... 34 0.16
sp|P05307|PDIA1_BOVIN Protein disulfide-isomerase precursor... 34 0.16
sp|P09103|PDIA1_MOUSE Protein disulfide-isomerase precursor... 34 0.21
>sp|O13811|PDI2_SCHPO Putative protein disulfide-isomerase C17H9.14c precursor
Length = 359
Score = 38.9 bits (89), Expect = 0.006
Identities = 24/90 (26%), Positives = 37/90 (41%), Gaps = 7/90 (7%)
Frame = +1
Query: 229 EPNKSTFVFIYAKWCRFSMAAAPIFNGLGRVF---PQLEFI---APAIKDFQEFKHTSYI 390
+ K V YA WC + AP + LG+VF P +E + A D +
Sbjct: 156 DDKKDVLVEFYADWCGYCKRLAPTYETLGKVFKNEPNVEIVKINADVFADIGRLHEVASF 215
Query: 391 PRIKMY-FGSKSVREFNGSDSDFDALVEFV 477
P IK + K E D ++L+E++
Sbjct: 216 PTIKFFPKDDKDKPELYEGDRSLESLIEYI 245
Score = 31.6 bits (70), Expect = 1.0
Identities = 24/92 (26%), Positives = 38/92 (41%), Gaps = 8/92 (8%)
Frame = +1
Query: 238 KSTFVFIYAKWCRFSMAAAPIFNGLGRVFPQLEFI------APAIKDFQEFKHTSYIPRI 399
K + YA WC + AP++ LG +F + A D + H + P +
Sbjct: 40 KGALIEFYATWCGHCKSLAPVYEELGALFEDHNDVLIGKIDADTHSDVADKYHITGFPTL 99
Query: 400 KMY--FGSKSVREFNGSDSDFDALVEFVYANT 489
+ GS+ V+ N D D+L +FV T
Sbjct: 100 IWFPPDGSEPVQYSNA--RDVDSLTQFVSEKT 129
>sp|P54399|PDI_DROME Protein disulfide-isomerase precursor (PDI)
Length = 496
Score = 37.0 bits (84), Expect = 0.025
Identities = 38/124 (30%), Positives = 55/124 (44%), Gaps = 7/124 (5%)
Frame = +1
Query: 136 VLQMDRPSEKDNIKAEMSSALNSSSEATKKNEPNKSTFVFIYAKWCRFSMAAAPIFNGLG 315
+L + P + D ++ + N S A K+ KS V YA WC API++ L
Sbjct: 355 LLSQELPEDWDKNPVKVLVSSNFESVALDKS---KSVLVEFYAPWCGHCKQLAPIYDQLA 411
Query: 316 RVFPQLEFIAPAIKD-----FQEFKHTSYIPRIKMYFGSK--SVREFNGSDSDFDALVEF 474
+ E I A D + K +S+ P IK YF + V +FN D D V+F
Sbjct: 412 EKYKDNEDIVIAKMDSTANELESIKISSF-PTIK-YFRKEDNKVIDFN-LDRTLDDFVKF 468
Query: 475 VYAN 486
+ AN
Sbjct: 469 LDAN 472
>sp|P09102|PDIA1_CHICK Protein disulfide-isomerase precursor (PDI) (Prolyl 4-hydroxylase
beta subunit) (Cellular thyroid hormone binding protein)
(Retina cognin) (R-cognin)
Length = 515
Score = 36.6 bits (83), Expect = 0.032
Identities = 26/81 (32%), Positives = 36/81 (44%), Gaps = 1/81 (1%)
Frame = +1
Query: 121 GKI-PLVLQMDRPSEKDNIKAEMSSALNSSSEATKKNEPNKSTFVFIYAKWCRFSMAAAP 297
GKI P ++ D P + D ++ N A + NK+ FV YA WC AP
Sbjct: 354 GKIKPHLMSQDLPEDWDKQPVKVLVGKNFEEVAF---DENKNVFVEFYAPWCGHCKQLAP 410
Query: 298 IFNGLGRVFPQLEFIAPAIKD 360
I++ LG + E I A D
Sbjct: 411 IWDKLGETYRDHENIVIAKMD 431
>sp|P38661|PDIA6_MEDSA Probable protein disulfide-isomerase A6 precursor (P5)
Length = 364
Score = 35.4 bits (80), Expect = 0.072
Identities = 23/86 (26%), Positives = 32/86 (37%), Gaps = 6/86 (6%)
Frame = +1
Query: 238 KSTFVFIYAKWCRFSMAAAPIFNGLGRVFPQLEFI------APAIKDFQEFKHTSYIPRI 399
K V YA WC + API+ + VF + + A +D E S P +
Sbjct: 166 KDVLVEFYAPWCGHCKSLAPIYEKVAAVFKSEDDVVIANLDADKYRDLAEKYDVSGFPTL 225
Query: 400 KMYFGSKSVREFNGSDSDFDALVEFV 477
K + E G D D V F+
Sbjct: 226 KFFPKGNKAGEDYGGGRDLDDFVAFI 251
>sp|P07237|PDIA1_HUMAN Protein disulfide-isomerase precursor (PDI) (Prolyl 4-hydroxylase
beta subunit) (Cellular thyroid hormone binding protein)
(p55)
Length = 508
Score = 34.3 bits (77), Expect = 0.16
Identities = 31/114 (27%), Positives = 48/114 (42%), Gaps = 7/114 (6%)
Frame = +1
Query: 121 GKI-PLVLQMDRPSEKDNIKAEMSSALNSSSEATKKNEPNKSTFVFIYAKWCRFSMAAAP 297
GKI P ++ + P + D ++ N A + K+ FV YA WC AP
Sbjct: 349 GKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAF---DEKKNVFVEFYAPWCGHCKQLAP 405
Query: 298 IFNGLGRVFPQLEFIAPAIKDFQ----EFKHTSYIPRIKMYFGS--KSVREFNG 441
I++ LG + E I A D E P +K + S ++V ++NG
Sbjct: 406 IWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNG 459
>sp|P04785|PDIA1_RAT Protein disulfide-isomerase precursor (PDI) (Prolyl 4-hydroxylase
beta subunit) (Cellular thyroid hormone binding protein)
Length = 509
Score = 34.3 bits (77), Expect = 0.16
Identities = 31/114 (27%), Positives = 48/114 (42%), Gaps = 7/114 (6%)
Frame = +1
Query: 121 GKI-PLVLQMDRPSEKDNIKAEMSSALNSSSEATKKNEPNKSTFVFIYAKWCRFSMAAAP 297
GKI P ++ + P + D ++ N A + K+ FV YA WC AP
Sbjct: 351 GKIKPHLMSQELPEDWDKQPVKVLVGKNFEEVAF---DEKKNVFVEFYAPWCGHCKQLAP 407
Query: 298 IFNGLGRVFPQLEFIAPAIKDFQ----EFKHTSYIPRIKMYFGS--KSVREFNG 441
I++ LG + E I A D E P +K + S ++V ++NG
Sbjct: 408 IWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNG 461
>sp|Q5R5B6|PDIA1_PONPY Protein disulfide-isomerase precursor (PDI) (Prolyl 4-hydroxylase
beta subunit)
Length = 508
Score = 34.3 bits (77), Expect = 0.16
Identities = 31/114 (27%), Positives = 48/114 (42%), Gaps = 7/114 (6%)
Frame = +1
Query: 121 GKI-PLVLQMDRPSEKDNIKAEMSSALNSSSEATKKNEPNKSTFVFIYAKWCRFSMAAAP 297
GKI P ++ + P + D ++ N A + K+ FV YA WC AP
Sbjct: 349 GKIKPHLMSQELPDDWDKQPVKVLVGKNFEDVAF---DEKKNVFVEFYAPWCGHCKQLAP 405
Query: 298 IFNGLGRVFPQLEFIAPAIKDFQ----EFKHTSYIPRIKMYFGS--KSVREFNG 441
I++ LG + E I A D E P +K + S ++V ++NG
Sbjct: 406 IWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNG 459
>sp|P12244|GSBP_CHICK Dolichyl-diphosphooligosaccharide-protein glycotransferase
precursor (Glycosylation site-binding chain) (GSBP)
Length = 508
Score = 34.3 bits (77), Expect = 0.16
Identities = 30/114 (26%), Positives = 51/114 (44%), Gaps = 7/114 (6%)
Frame = +1
Query: 121 GKIPLVLQMDRPSEKDNIKAEMSSALNSSSEATKKNEPNKSTFVFIYAKWCRFSMAAAPI 300
GKI L + P +D K + + + E +E K+ F+ YA WC AP+
Sbjct: 349 GKIKPHLMSNEPLPEDWDKQPVKVLVGKNYEEVAFDE-KKNVFIEFYAPWCGHCKQLAPM 407
Query: 301 FNGLGRVFPQLEFIAPA-----IKDFQEFKHTSYIPRIKMYFGS--KSVREFNG 441
++ LG + E I A + + K S+ P +K + S ++V ++NG
Sbjct: 408 WDRLGEAYKDDENIVIAKMESTANEVEAIKVHSF-PTLKFFPASAERTVIDYNG 460
>sp|P05307|PDIA1_BOVIN Protein disulfide-isomerase precursor (PDI) (Prolyl 4-hydroxylase
beta subunit) (Cellular thyroid hormone binding protein)
(p55)
Length = 510
Score = 34.3 bits (77), Expect = 0.16
Identities = 31/114 (27%), Positives = 48/114 (42%), Gaps = 7/114 (6%)
Frame = +1
Query: 121 GKI-PLVLQMDRPSEKDNIKAEMSSALNSSSEATKKNEPNKSTFVFIYAKWCRFSMAAAP 297
GKI P ++ + P + D ++ N A + K+ FV YA WC AP
Sbjct: 351 GKIKPHLMSQELPDDWDKQPVKVLVGKNFEEVAF---DEKKNVFVEFYAPWCGHCKQLAP 407
Query: 298 IFNGLGRVFPQLEFIAPAIKDFQ----EFKHTSYIPRIKMYFGS--KSVREFNG 441
I++ LG + E I A D E P +K + S ++V ++NG
Sbjct: 408 IWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNG 461
>sp|P09103|PDIA1_MOUSE Protein disulfide-isomerase precursor (PDI) (Prolyl 4-hydroxylase
beta subunit) (Cellular thyroid hormone binding protein)
(p55) (Erp59)
Length = 509
Score = 33.9 bits (76), Expect = 0.21
Identities = 31/114 (27%), Positives = 48/114 (42%), Gaps = 7/114 (6%)
Frame = +1
Query: 121 GKI-PLVLQMDRPSEKDNIKAEMSSALNSSSEATKKNEPNKSTFVFIYAKWCRFSMAAAP 297
GKI P ++ + P + D ++ N A + K+ FV YA WC AP
Sbjct: 351 GKIKPHLMSQEVPEDWDKQPVKVLVGANFEEVAF---DEKKNVFVEFYAPWCGHCKQLAP 407
Query: 298 IFNGLGRVFPQLEFIAPAIKDFQ----EFKHTSYIPRIKMYFGS--KSVREFNG 441
I++ LG + E I A D E P +K + S ++V ++NG
Sbjct: 408 IWDKLGETYKDHENIIIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNG 461
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 53,975,087
Number of Sequences: 369166
Number of extensions: 1018409
Number of successful extensions: 3303
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3267
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3303
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 2970720600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)