Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_005_J20-2
(647 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P20232|TFS2_DROME Transcription elongation factor S-II (... 168 1e-41
sp|P52652|TFS2_CAEEL Putative transcription elongation fact... 164 2e-40
sp|Q15560|TCEA2_HUMAN Transcription elongation factor A pro... 162 5e-40
sp|Q4KLL0|TCEA1_RAT Transcription elongation factor A prote... 158 1e-38
sp|P23193|TCEA1_HUMAN Transcription elongation factor A pro... 158 1e-38
sp|P10711|TCEA1_MOUSE Transcription elongation factor A pro... 158 1e-38
sp|Q63799|TCEA2_RAT Transcription elongation factor A prote... 157 2e-38
sp|Q9QVN7|TCEA2_MOUSE Transcription elongation factor A pro... 157 2e-38
sp|P23881|TCEA3_MOUSE Transcription elongation factor A pro... 152 9e-37
sp|O75764|TCEA3_HUMAN Transcription elongation factor A pro... 149 6e-36
>sp|P20232|TFS2_DROME Transcription elongation factor S-II (RNA polymerase II elongation
factor DMS-II) (TFIIS)
Length = 313
Score = 168 bits (426), Expect = 1e-41
Identities = 76/144 (52%), Positives = 108/144 (75%)
Frame = +1
Query: 1 VPEGSYDTEYLSVQIESAIFNVFMNSGPTYKQRVRTRVMNLRDQNNVELRLNVLAGTVSA 180
VPEG + E ++ ++E AI++ F N+ YK R+R+RV NL+D N LR N + G V+A
Sbjct: 170 VPEGCGEPEEMAAELEDAIYSEFNNTDMKYKNRIRSRVANLKDPKNPGLRGNFMCGAVTA 229
Query: 181 EKLALMSSEDMASKEMKEMRKKYMGEVIHESQLCIGGGTETDLIKCPKCRESRCSYNQVQ 360
++LA M+ E+MAS EMK++R+K++ E I+++QL GT+TDL+KC KC++ C+YNQ+Q
Sbjct: 230 KQLAKMTPEEMASDEMKKLREKFVKEAINDAQLATVQGTKTDLLKCAKCKKRNCTYNQLQ 289
Query: 361 TRSADEPMTTFAFCNSCGHRWKFC 432
TRSADEPMTTF CN CG+RWKFC
Sbjct: 290 TRSADEPMTTFVMCNECGNRWKFC 313
>sp|P52652|TFS2_CAEEL Putative transcription elongation factor S-II (TFIIS)
Length = 308
Score = 164 bits (414), Expect = 2e-40
Identities = 75/144 (52%), Positives = 103/144 (71%)
Frame = +1
Query: 1 VPEGSYDTEYLSVQIESAIFNVFMNSGPTYKQRVRTRVMNLRDQNNVELRLNVLAGTVSA 180
+P+G+ D E L+VQIE +++V ++ +Y VR+R+ NLRD+ N+ LR NVL G V A
Sbjct: 165 MPQGTLDPEELAVQIEEKLYSVHRDTNKSYSAAVRSRIFNLRDKKNLALRENVLTGVVRA 224
Query: 181 EKLALMSSEDMASKEMKEMRKKYMGEVIHESQLCIGGGTETDLIKCPKCRESRCSYNQVQ 360
EK A M+SE+MAS E++EMR K+ E I E Q+ + GT +D+ KC KC + C+Y Q+Q
Sbjct: 225 EKFATMTSEEMASAEIREMRDKFTKEAILEHQMSVQQGTPSDMFKCGKCGKKNCTYTQLQ 284
Query: 361 TRSADEPMTTFAFCNSCGHRWKFC 432
TRS+DEPMTTF FC CG+RWKFC
Sbjct: 285 TRSSDEPMTTFVFCLECGNRWKFC 308
>sp|Q15560|TCEA2_HUMAN Transcription elongation factor A protein 2 (Transcription
elongation factor S-II protein 2) (Testis-specific S-II)
(Transcription elongation factor TFIIS.l)
Length = 299
Score = 162 bits (411), Expect = 5e-40
Identities = 76/138 (55%), Positives = 97/138 (70%)
Frame = +1
Query: 19 DTEYLSVQIESAIFNVFMNSGPTYKQRVRTRVMNLRDQNNVELRLNVLAGTVSAEKLALM 198
D E LS QIE IF N+ YK RVR+R+ NL+D N +LR NVL G ++ +++A+M
Sbjct: 162 DCERLSAQIEECIFRDVGNTDMKYKNRVRSRISNLKDAKNPDLRRNVLCGAITPQQIAVM 221
Query: 199 SSEDMASKEMKEMRKKYMGEVIHESQLCIGGGTETDLIKCPKCRESRCSYNQVQTRSADE 378
+SE+MAS E+KE+RK E I E Q+ GGT+TDL C KCR+ C+Y QVQTRS+DE
Sbjct: 222 TSEEMASDELKEIRKAMTKEAIREHQMARTGGTQTDLFTCGKCRKKNCTYTQVQTRSSDE 281
Query: 379 PMTTFAFCNSCGHRWKFC 432
PMTTF CN CG+RWKFC
Sbjct: 282 PMTTFVVCNECGNRWKFC 299
>sp|Q4KLL0|TCEA1_RAT Transcription elongation factor A protein 1 (Transcription
elongation factor S-II protein 1)
Length = 301
Score = 158 bits (400), Expect = 1e-38
Identities = 75/138 (54%), Positives = 92/138 (66%)
Frame = +1
Query: 19 DTEYLSVQIESAIFNVFMNSGPTYKQRVRTRVMNLRDQNNVELRLNVLAGTVSAEKLALM 198
D E L QIE AI+ N+ YK RVR+R+ NL+D N LR NVL G + + A M
Sbjct: 164 DEEELGSQIEEAIYQEIRNTDMKYKNRVRSRISNLKDAKNPNLRKNVLCGNIPPDLFARM 223
Query: 199 SSEDMASKEMKEMRKKYMGEVIHESQLCIGGGTETDLIKCPKCRESRCSYNQVQTRSADE 378
++E+MAS E+KEMRK E I E Q+ GGT+TDL C KC++ C+Y QVQTRSADE
Sbjct: 224 TAEEMASDELKEMRKNLTKEAIREHQMAKTGGTQTDLFTCGKCKKKNCTYTQVQTRSADE 283
Query: 379 PMTTFAFCNSCGHRWKFC 432
PMTTF CN CG+RWKFC
Sbjct: 284 PMTTFVVCNECGNRWKFC 301
>sp|P23193|TCEA1_HUMAN Transcription elongation factor A protein 1 (Transcription
elongation factor S-II protein 1) (Transcription
elongation factor TFIIS.o)
Length = 301
Score = 158 bits (400), Expect = 1e-38
Identities = 75/138 (54%), Positives = 92/138 (66%)
Frame = +1
Query: 19 DTEYLSVQIESAIFNVFMNSGPTYKQRVRTRVMNLRDQNNVELRLNVLAGTVSAEKLALM 198
D E L QIE AI+ N+ YK RVR+R+ NL+D N LR NVL G + + A M
Sbjct: 164 DEEELGSQIEEAIYQEIRNTDMKYKNRVRSRISNLKDAKNPNLRKNVLCGNIPPDLFARM 223
Query: 199 SSEDMASKEMKEMRKKYMGEVIHESQLCIGGGTETDLIKCPKCRESRCSYNQVQTRSADE 378
++E+MAS E+KEMRK E I E Q+ GGT+TDL C KC++ C+Y QVQTRSADE
Sbjct: 224 TAEEMASDELKEMRKNLTKEAIREHQMAKTGGTQTDLFTCGKCKKKNCTYTQVQTRSADE 283
Query: 379 PMTTFAFCNSCGHRWKFC 432
PMTTF CN CG+RWKFC
Sbjct: 284 PMTTFVVCNECGNRWKFC 301
>sp|P10711|TCEA1_MOUSE Transcription elongation factor A protein 1 (Transcription
elongation factor S-II protein 1) (Transcription
elongation factor TFIIS.o)
Length = 301
Score = 158 bits (400), Expect = 1e-38
Identities = 75/138 (54%), Positives = 92/138 (66%)
Frame = +1
Query: 19 DTEYLSVQIESAIFNVFMNSGPTYKQRVRTRVMNLRDQNNVELRLNVLAGTVSAEKLALM 198
D E L QIE AI+ N+ YK RVR+R+ NL+D N LR NVL G + + A M
Sbjct: 164 DEEELGSQIEEAIYQEIRNTDMKYKNRVRSRISNLKDAKNPNLRKNVLCGNIPPDLFARM 223
Query: 199 SSEDMASKEMKEMRKKYMGEVIHESQLCIGGGTETDLIKCPKCRESRCSYNQVQTRSADE 378
++E+MAS E+KEMRK E I E Q+ GGT+TDL C KC++ C+Y QVQTRSADE
Sbjct: 224 TAEEMASDELKEMRKNLTKEAIREHQMAKTGGTQTDLFTCGKCKKKNCTYTQVQTRSADE 283
Query: 379 PMTTFAFCNSCGHRWKFC 432
PMTTF CN CG+RWKFC
Sbjct: 284 PMTTFVVCNECGNRWKFC 301
>sp|Q63799|TCEA2_RAT Transcription elongation factor A protein 2 (Transcription
elongation factor S-II protein 2) (Testis-specific S-II)
(Protein S-II-T1) (Transcription elongation factor
TFIIS.l)
Length = 299
Score = 157 bits (398), Expect = 2e-38
Identities = 74/136 (54%), Positives = 96/136 (70%)
Frame = +1
Query: 25 EYLSVQIESAIFNVFMNSGPTYKQRVRTRVMNLRDQNNVELRLNVLAGTVSAEKLALMSS 204
E+LS QIE IF N+ YK RVR+R+ NL+D N LR NVL G ++ +++A+M+S
Sbjct: 164 EHLSSQIEECIFLDVGNTDMKYKNRVRSRISNLKDAKNPGLRRNVLCGAITPQQIAVMTS 223
Query: 205 EDMASKEMKEMRKKYMGEVIHESQLCIGGGTETDLIKCPKCRESRCSYNQVQTRSADEPM 384
E+MAS E+KE+RK E I E Q+ GGT+TDL C KCR+ C+Y QVQTRS+DEPM
Sbjct: 224 EEMASDELKEIRKAMTKEAIREHQMARTGGTQTDLFTCNKCRKKNCTYTQVQTRSSDEPM 283
Query: 385 TTFAFCNSCGHRWKFC 432
TT+ CN CG+RWKFC
Sbjct: 284 TTYVVCNECGNRWKFC 299
>sp|Q9QVN7|TCEA2_MOUSE Transcription elongation factor A protein 2 (Transcription
elongation factor S-II protein 2) (Testis-specific S-II)
(Protein S-II-T1) (Transcription elongation factor
TFIIS.l)
Length = 299
Score = 157 bits (398), Expect = 2e-38
Identities = 74/136 (54%), Positives = 96/136 (70%)
Frame = +1
Query: 25 EYLSVQIESAIFNVFMNSGPTYKQRVRTRVMNLRDQNNVELRLNVLAGTVSAEKLALMSS 204
E+LS QIE IF N+ YK RVR+R+ NL+D N LR NVL G ++ +++A+M+S
Sbjct: 164 EHLSSQIEECIFLDVGNTDMKYKNRVRSRISNLKDAKNPGLRRNVLCGAITPQQIAVMTS 223
Query: 205 EDMASKEMKEMRKKYMGEVIHESQLCIGGGTETDLIKCPKCRESRCSYNQVQTRSADEPM 384
E+MAS E+KE+RK E I E Q+ GGT+TDL C KCR+ C+Y QVQTRS+DEPM
Sbjct: 224 EEMASDELKEIRKAMTKEAIREHQMARTGGTQTDLFTCNKCRKKNCTYTQVQTRSSDEPM 283
Query: 385 TTFAFCNSCGHRWKFC 432
TT+ CN CG+RWKFC
Sbjct: 284 TTYVVCNECGNRWKFC 299
>sp|P23881|TCEA3_MOUSE Transcription elongation factor A protein 3 (Transcription
elongation factor S-II protein 3) (Transcription
elongation factor TFIIS.h)
Length = 347
Score = 152 bits (383), Expect = 9e-37
Identities = 69/134 (51%), Positives = 94/134 (70%)
Frame = +1
Query: 31 LSVQIESAIFNVFMNSGPTYKQRVRTRVMNLRDQNNVELRLNVLAGTVSAEKLALMSSED 210
L+ +IE I+ ++ Y+ RVR+R+ NL+D N LR NVL+G +S E +A M++E+
Sbjct: 214 LASEIEDHIYQELKSTDMKYRNRVRSRISNLKDPRNPGLRRNVLSGAISPELIAKMTAEE 273
Query: 211 MASKEMKEMRKKYMGEVIHESQLCIGGGTETDLIKCPKCRESRCSYNQVQTRSADEPMTT 390
MAS E++E+R E I E Q+ GGT TDL++C KC++ C+YNQVQTRSADEPMTT
Sbjct: 274 MASDELRELRNAMTQEAIREHQMAKTGGTTTDLLRCSKCKKKNCTYNQVQTRSADEPMTT 333
Query: 391 FAFCNSCGHRWKFC 432
F CN CG+RWKFC
Sbjct: 334 FVLCNECGNRWKFC 347
>sp|O75764|TCEA3_HUMAN Transcription elongation factor A protein 3 (Transcription
elongation factor S-II protein 3) (Transcription
elongation factor TFIIS.h)
Length = 348
Score = 149 bits (376), Expect = 6e-36
Identities = 68/134 (50%), Positives = 93/134 (69%)
Frame = +1
Query: 31 LSVQIESAIFNVFMNSGPTYKQRVRTRVMNLRDQNNVELRLNVLAGTVSAEKLALMSSED 210
++ +IE I+ ++ Y+ RVR+R+ NL+D N LR NVL+G +SA +A M++E+
Sbjct: 215 MASEIEDHIYQELKSTDMKYRNRVRSRISNLKDPRNPGLRRNVLSGAISAGLIAKMTAEE 274
Query: 211 MASKEMKEMRKKYMGEVIHESQLCIGGGTETDLIKCPKCRESRCSYNQVQTRSADEPMTT 390
MAS E++E+R E I E Q+ GGT TDL +C KC++ C+YNQVQTRSADEPMTT
Sbjct: 275 MASDELRELRNAMTQEAIREHQMAKTGGTTTDLFQCSKCKKKNCTYNQVQTRSADEPMTT 334
Query: 391 FAFCNSCGHRWKFC 432
F CN CG+RWKFC
Sbjct: 335 FVLCNECGNRWKFC 348
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 79,225,008
Number of Sequences: 369166
Number of extensions: 1643308
Number of successful extensions: 5236
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5040
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5233
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 5316264630
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)