Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_005_I02
(628 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9DBI0|TMPS6_MOUSE Transmembrane protease, serine 6 (Mat... 100 4e-21
sp|P26262|KLKB1_MOUSE Plasma kallikrein precursor (Plasma p... 93 5e-19
sp|Q8IU80|TMPS6_HUMAN Transmembrane protease, serine 6 (Mat... 92 8e-19
sp|P14272|KLKB1_RAT Plasma kallikrein precursor (Plasma pre... 90 4e-18
sp|P03952|KLKB1_HUMAN Plasma kallikrein precursor (Plasma p... 86 6e-17
sp|Q5QSK2|DESC4_RAT Serine protease DESC4 precursor [Contai... 83 5e-16
sp|Q9QYZ9|TMSP8_MOUSE Transmembrane serine protease 8 precu... 83 7e-16
sp|Q5K2P8|POLS2_MOUSE Polyserase-2 precursor (Polyserine pr... 82 1e-15
sp|Q5U405|TMPSD_MOUSE Transmembrane protease, serine 13 (Mo... 82 1e-15
sp|P19221|THRB_MOUSE Prothrombin precursor (Coagulation fac... 81 2e-15
>sp|Q9DBI0|TMPS6_MOUSE Transmembrane protease, serine 6 (Matriptase-2)
Length = 799
Score = 100 bits (248), Expect = 4e-21
Identities = 66/191 (34%), Positives = 101/191 (52%), Gaps = 5/191 (2%)
Frame = +3
Query: 6 FIINPLFRADY---SSNLYDIALLKLDKPVIPSKVVNINFPCVPQVKNYTLDKSAKCYAI 176
F ++ LF Y S+ YD+ALL+LD PV+ S V C+P +++ + C+
Sbjct: 637 FKVSRLFLHPYHEEDSHDYDVALLQLDHPVVYSATVRP--VCLP-ARSHFFEPGQHCWIT 693
Query: 177 GWGNIEQNEPYNIWSFEFDFVLERWRKNKSQKNSNILQEVEIPLVNFELCQKRYRIFNLN 356
GWG + P SN LQ+V++ LV +LC + YR + ++
Sbjct: 694 GWGAQREGGPV----------------------SNTLQKVDVQLVPQDLCSEAYR-YQVS 730
Query: 357 KEIHICAGSRR--KDTCRGDSGGGLFCLDPSTNRWQLYGITNFGSARGCGTSYGVYTRVP 530
+ +CAG R+ KD C+GDSGG L C +PS RW L G+ ++G G +GVYTRV
Sbjct: 731 PRM-LCAGYRKGKKDACQGDSGGPLVCREPS-GRWFLAGLVSWGLGCGRPNFFGVYTRVT 788
Query: 531 EVSNWIKEIIS 563
V NWI+++++
Sbjct: 789 RVINWIQQVLT 799
>sp|P26262|KLKB1_MOUSE Plasma kallikrein precursor (Plasma prekallikrein) (Kininogenin)
(Fletcher factor) [Contains: Plasma kallikrein heavy
chain; Plasma kallikrein light chain]
Length = 638
Score = 93.2 bits (230), Expect = 5e-19
Identities = 65/175 (37%), Positives = 85/175 (48%), Gaps = 3/175 (1%)
Frame = +3
Query: 39 SSNLYDIALLKLDKPVIPSKVVNINFP-CVPQVKNYTLDKSAKCYAIGWGNIEQNEPYNI 215
S YDIAL+KL P+ P C+P K T C+ GWG ++
Sbjct: 478 SEGNYDIALIKLQTPL---NYTEFQKPICLPS-KADTNTIYTNCWVTGWGYTKEQG---- 529
Query: 216 WSFEFDFVLERWRKNKSQKNSNILQEVEIPLVNFELCQKRYRIFNLNKEIHICAGSRR-- 389
+ NILQ+ IPLV E CQK+YR + +NK++ ICAG +
Sbjct: 530 ------------------ETQNILQKATIPLVPNEECQKKYRDYVINKQM-ICAGYKEGG 570
Query: 390 KDTCRGDSGGGLFCLDPSTNRWQLYGITNFGSARGCGTSYGVYTRVPEVSNWIKE 554
D C+GDSGG L C + RWQL GIT++G G GVYT+V E +WI E
Sbjct: 571 TDACKGDSGGPLVC--KHSGRWQLVGITSWGEGCGRKDQPGVYTKVSEYMDWILE 623
>sp|Q8IU80|TMPS6_HUMAN Transmembrane protease, serine 6 (Matriptase-2)
Length = 802
Score = 92.4 bits (228), Expect = 8e-19
Identities = 60/187 (32%), Positives = 98/187 (52%), Gaps = 2/187 (1%)
Frame = +3
Query: 9 IINPLFRADYSSNLYDIALLKLDKPVIPSKVVNINFPCVPQVKNYTLDKSAKCYAIGWGN 188
+++P D S+ YD+ALL+LD PV+ S V C+P +++ + C+ GWG
Sbjct: 646 LLHPYHEED--SHDYDVALLQLDHPVVRSAAVRP--VCLP-ARSHFFEPGLHCWITGWGA 700
Query: 189 IEQNEPYNIWSFEFDFVLERWRKNKSQKNSNILQEVEIPLVNFELCQKRYRIFNLNKEIH 368
+ + P SN LQ+V++ L+ +LC + YR + + +
Sbjct: 701 LREGGPI----------------------SNALQKVDVQLIPQDLCSEVYR-YQVTPRM- 736
Query: 369 ICAGSRR--KDTCRGDSGGGLFCLDPSTNRWQLYGITNFGSARGCGTSYGVYTRVPEVSN 542
+CAG R+ KD C+GDSGG L C S RW L G+ ++G G +GVYTR+ V +
Sbjct: 737 LCAGYRKGKKDACQGDSGGPLVCKALS-GRWFLAGLVSWGLGCGRPNYFGVYTRITGVIS 795
Query: 543 WIKEIIS 563
WI+++++
Sbjct: 796 WIQQVVT 802
>sp|P14272|KLKB1_RAT Plasma kallikrein precursor (Plasma prekallikrein) (Kininogenin)
(Fletcher factor) [Contains: Plasma kallikrein heavy
chain; Plasma kallikrein light chain]
Length = 638
Score = 90.1 bits (222), Expect = 4e-18
Identities = 65/180 (36%), Positives = 85/180 (47%), Gaps = 3/180 (1%)
Frame = +3
Query: 39 SSNLYDIALLKLDKPVIPSKVVNINFP-CVPQVKNYTLDKSAKCYAIGWGNIEQNEPYNI 215
S YDIAL+KL P+ P C+P K T C+ GWG ++
Sbjct: 478 SEGSYDIALIKLQTPL---NYTEFQKPICLPS-KADTNTIYTNCWVTGWGYTKERG---- 529
Query: 216 WSFEFDFVLERWRKNKSQKNSNILQEVEIPLVNFELCQKRYRIFNLNKEIHICAGSRRK- 392
+ NILQ+ IPLV E CQK+YR + + K++ ICAG +
Sbjct: 530 ------------------ETQNILQKATIPLVPNEECQKKYRDYVITKQM-ICAGYKEGG 570
Query: 393 -DTCRGDSGGGLFCLDPSTNRWQLYGITNFGSARGCGTSYGVYTRVPEVSNWIKEIISKS 569
D C+GDSGG L C + RWQL GIT++G GVYT+V E +WI E I S
Sbjct: 571 IDACKGDSGGPLVC--KHSGRWQLVGITSWGEGCARKEQPGVYTKVAEYIDWILEKIQSS 628
>sp|P03952|KLKB1_HUMAN Plasma kallikrein precursor (Plasma prekallikrein) (Kininogenin)
(Fletcher factor) [Contains: Plasma kallikrein heavy
chain; Plasma kallikrein light chain]
Length = 638
Score = 86.3 bits (212), Expect = 6e-17
Identities = 62/175 (35%), Positives = 83/175 (47%), Gaps = 3/175 (1%)
Frame = +3
Query: 39 SSNLYDIALLKLDKPVIPSKVVNINFP-CVPQVKNYTLDKSAKCYAIGWGNIEQNEPYNI 215
S +DIAL+KL P+ P C+P K T C+ GWG
Sbjct: 478 SEGNHDIALIKLQAPL---NYTEFQKPICLPS-KGDTSTIYTNCWVTGWG---------- 523
Query: 216 WSFEFDFVLERWRKNKSQKNSNILQEVEIPLVNFELCQKRYRIFNLNKEIHICAGSRR-- 389
+ K K + NILQ+V IPLV E CQKRY+ + + + + +CAG +
Sbjct: 524 -----------FSKEKGEIQ-NILQKVNIPLVTNEECQKRYQDYKITQRM-VCAGYKEGG 570
Query: 390 KDTCRGDSGGGLFCLDPSTNRWQLYGITNFGSARGCGTSYGVYTRVPEVSNWIKE 554
KD C+GDSGG L C W+L GIT++G GVYT+V E +WI E
Sbjct: 571 KDACKGDSGGPLVC--KHNGMWRLVGITSWGEGCARREQPGVYTKVAEYMDWILE 623
>sp|Q5QSK2|DESC4_RAT Serine protease DESC4 precursor [Contains: Serine protease DESC4
non-catalytic chain; Serine protease DESC4 catalytic
chain]
Length = 417
Score = 83.2 bits (204), Expect = 5e-16
Identities = 57/168 (33%), Positives = 81/168 (48%), Gaps = 2/168 (1%)
Frame = +3
Query: 54 DIALLKLDKPVIPSKVVNINFPCVPQVKNYTLDKSAKCYAIGWGNIEQNEPYNIWSFEFD 233
DIA++KL PV+ S+ N+ C+P+ L KS K + GWG ++ N P+
Sbjct: 271 DIAVVKLSSPVLFSE--NLRTVCLPEATFQVLPKS-KVFVTGWGALKANGPF-------- 319
Query: 234 FVLERWRKNKSQKNSNILQEVEIPLVNFELCQKRYRIFNLNKEIHICAG--SRRKDTCRG 407
N LQEVEI +++ ++C + ICAG + + D C G
Sbjct: 320 --------------PNSLQEVEIEIISNDVCNQVNVYGGAISSGMICAGFLTGKLDACEG 365
Query: 408 DSGGGLFCLDPSTNRWQLYGITNFGSARGCGTSYGVYTRVPEVSNWIK 551
DSGG L D + N+W L GI ++G G G+YTRV NWIK
Sbjct: 366 DSGGPLVISD-NRNKWYLLGIVSWGIDCGKENKPGIYTRVTHYRNWIK 412
>sp|Q9QYZ9|TMSP8_MOUSE Transmembrane serine protease 8 precursor (Distal intestinal serine
protease)
Length = 310
Score = 82.8 bits (203), Expect = 7e-16
Identities = 61/194 (31%), Positives = 97/194 (50%), Gaps = 11/194 (5%)
Frame = +3
Query: 30 ADYSSNLYDIALLKLDKPVIPSKVVNINFPCVPQVKNYTLDKSAKCYAIGWGNIEQNEPY 209
AD SS DIAL++LD P+ PS+ + C+P + L C+ GWG ++ +
Sbjct: 122 ADASSG--DIALVQLDTPLRPSQFTPV---CLPAAQT-PLTPGTVCWVTGWGATQERDM- 174
Query: 210 NIWSFEFDFVLERWRKNKSQKNSNILQEVEIPLVNFELCQKRYRI-------FNLNKEIH 368
+++LQE+ +PL++ E C+K Y + +
Sbjct: 175 ----------------------ASVLQELAVPLLDSEDCEKMYHTQGSSLSGERIIQSDM 212
Query: 369 ICAG--SRRKDTCRGDSGGGLFCLDPSTNRWQLYGITNFGSARGCGTSY--GVYTRVPEV 536
+CAG +KD+C+GDSGG L C S+ W GIT++G GC Y GVYTRVP
Sbjct: 213 LCAGYVEGQKDSCQGDSGGPLVCSINSS--WTQVGITSWGI--GCARPYRPGVYTRVPTY 268
Query: 537 SNWIKEIISKSANE 578
+WI+ I++++ ++
Sbjct: 269 VDWIQRILAENHSD 282
>sp|Q5K2P8|POLS2_MOUSE Polyserase-2 precursor (Polyserine protease-2) (Protease serine 36)
Length = 849
Score = 82.0 bits (201), Expect = 1e-15
Identities = 60/180 (33%), Positives = 89/180 (49%), Gaps = 8/180 (4%)
Frame = +3
Query: 54 DIALLKLDKPVIPSKVVNINFPCVPQVKNYTLDKSAKCYAIGWGNIEQNEPYNIWSFEFD 233
D+ALL+L P ++ C+P+ + +A C+A GWG++++++P +
Sbjct: 139 DLALLRLASPAKLGP--SVKPVCLPRASHLFAHGTA-CWATGWGDVQESDPLPV------ 189
Query: 234 FVLERWRKNKSQKNSNILQEVEIPLVNFELCQKRYRI---FNLNKEI---HICAG--SRR 389
W +LQEVE+ L+ CQ Y FNL ++ +CAG R
Sbjct: 190 ----PW----------VLQEVELKLLGETACQCLYSRPGPFNLTLQLLPGMLCAGYPEGR 235
Query: 390 KDTCRGDSGGGLFCLDPSTNRWQLYGITNFGSARGCGTSYGVYTRVPEVSNWIKEIISKS 569
+DTC+GDSGG L C D RW L GIT+FG G GV+T V +WI+E + S
Sbjct: 236 RDTCQGDSGGPLVCEDGG--RWFLAGITSFGFGCGRRNRPGVFTAVAHYESWIREHVMGS 293
>sp|Q5U405|TMPSD_MOUSE Transmembrane protease, serine 13 (Mosaic serine protease)
(Membrane-type mosaic serine protease)
Length = 543
Score = 82.0 bits (201), Expect = 1e-15
Identities = 54/168 (32%), Positives = 84/168 (50%), Gaps = 2/168 (1%)
Frame = +3
Query: 51 YDIALLKLDKPVIPSKVVNINFPCVPQVKNYTLDKSAKCYAIGWGNIEQNEPYNIWSFEF 230
YDIAL++L KP+ S +I+ C+P + T + C+ G+G ++ +
Sbjct: 389 YDIALIRLSKPLTLS--AHIHPACLP-MHGQTFGLNETCWITGFGKTKETD--------- 436
Query: 231 DFVLERWRKNKSQKNSNILQEVEIPLVNFELCQKRYRIFNLNKEIHICAGSRR--KDTCR 404
+K S L+EV++ L++F+ C + +CAG R +D+C+
Sbjct: 437 ------------EKTSPFLREVQVNLIDFKKCNDYLVYDSYLTPRMMCAGDLRGGRDSCQ 484
Query: 405 GDSGGGLFCLDPSTNRWQLYGITNFGSARGCGTSYGVYTRVPEVSNWI 548
GDSGG L C NRW L G+T++G+ G GVYT+V EV WI
Sbjct: 485 GDSGGPLVC--EQNNRWYLAGVTSWGTGCGQKNKPGVYTKVTEVLPWI 530
>sp|P19221|THRB_MOUSE Prothrombin precursor (Coagulation factor II) [Contains: Activation
peptide fragment 1; Activation peptide fragment 2;
Thrombin light chain; Thrombin heavy chain]
Length = 618
Score = 81.3 bits (199), Expect = 2e-15
Identities = 60/188 (31%), Positives = 85/188 (45%), Gaps = 8/188 (4%)
Frame = +3
Query: 27 RADYSSNL-YDIALLKLDKPVIPSKVVNINFPCVPQVKNYT--LDKSAKCYAIGWGNIEQ 197
R ++ NL DIALLKL KPV S ++ C+P + T L K GWGN+
Sbjct: 449 RYNWRENLDRDIALLKLKKPVPFSDYIHP--VCLPDKQTVTSLLRAGYKGRVTGWGNLR- 505
Query: 198 NEPYNIWSFEFDFVLERWRKNKSQKNSNILQEVEIPLVNFELCQKRYRIFNLNKEIHICA 377
E W N ++ ++LQ V +P+V +C+ RI + CA
Sbjct: 506 ---------------ETWTTNINEIQPSVLQVVNLPIVERPVCKASTRIRITDNMF--CA 548
Query: 378 G-----SRRKDTCRGDSGGGLFCLDPSTNRWQLYGITNFGSARGCGTSYGVYTRVPEVSN 542
G ++R D C GDSGG P NRW GI ++G YG YT V +
Sbjct: 549 GFKVNDTKRGDACEGDSGGPFVMKSPFNNRWYQMGIVSWGEGCDRKGKYGFYTHVFRLKR 608
Query: 543 WIKEIISK 566
WI+++I +
Sbjct: 609 WIQKVIDQ 616
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 74,728,729
Number of Sequences: 369166
Number of extensions: 1632160
Number of successful extensions: 5231
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4466
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4605
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 4974853140
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)