Planarian EST Database


Dr_sW_005_G19

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_005_G19
         (582 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P53073|YGY1_YEAST  Hypothetical 21.5 kDa protein in SEC15...    80   5e-15
sp|P57292|PANC_BUCAI  Pantoate--beta-alanine ligase (Pantoth...    31   2.6  
sp|O51314|RL13_BORBU  50S ribosomal protein L13                    30   5.7  
sp|P00548|KPYK_CHICK  Pyruvate kinase, muscle isozyme              29   7.4  
sp|Q5ZKK1|MARE2_CHICK  Microtubule-associated protein RP/EB ...    29   9.7  
sp|P16453|DCHS_RAT  Histidine decarboxylase (HDC)                  29   9.7  
sp|Q9UIS9|MBD1_HUMAN  Methyl-CpG-binding domain protein 1 (M...    29   9.7  
>sp|P53073|YGY1_YEAST Hypothetical 21.5 kDa protein in SEC15-SAP4 intergenic region
          Length = 190

 Score = 79.7 bits (195), Expect = 5e-15
 Identities = 46/144 (31%), Positives = 72/144 (50%), Gaps = 20/144 (13%)
 Frame = +1

Query: 94  LPSPVGYSDRSLPSSSVRKNDSD---------------LMVKRSWDIALGPIKQIPMNLI 228
           LPSP G+   S   +  RK                   L V+++W IAL P K IPMN+ 
Sbjct: 30  LPSPPGFEGNSSKGNVTRKQQDATSQTTSLAQKNQITVLQVQKAWQIALQPAKSIPMNIF 89

Query: 229 LMWMAGNTISIFPIVMVFMMFLRPIQAIFSMQSTFQVIEGSAAS-----IQCFVYFLGNV 393
           + +M+G ++ I PI+   M+   PI+AIFS +S F+ + G+ A+        F+Y +   
Sbjct: 90  MSYMSGTSLQIIPIMTALMLLSGPIKAIFSTRSAFKPVLGNKATQSQVQTAMFMYIVFQG 149

Query: 394 LNLGLAVYKCHIMGLLPTFASDWL 465
           + + +   K + MGL+P    DWL
Sbjct: 150 VLMYIGYRKLNSMGLIPNAKGDWL 173
>sp|P57292|PANC_BUCAI Pantoate--beta-alanine ligase (Pantothenate synthetase)
           (Pantoate-activating enzyme)
          Length = 285

 Score = 30.8 bits (68), Expect = 2.6
 Identities = 18/80 (22%), Positives = 41/80 (51%)
 Frame = -3

Query: 568 VLKRKINILNY*MKIITLKFLQLTTRLS*EVQQKPTNLRQRWVIDPLYDIYTQLILSLIH 389
           ++K  +  LNY +KII+L  ++L   L+   +    + ++  +   LY I  +    +I 
Sbjct: 158 IIKILVKELNYMIKIISLPTIRLKNGLALSSRNNYLSSQENEIAPYLYKIIKKTCEKIIK 217

Query: 388 YQENIQSIVLTQQNLLSLEK 329
             +NI+  ++ +  +L ++K
Sbjct: 218 EDDNIRPKIIHESKILLIKK 237
>sp|O51314|RL13_BORBU 50S ribosomal protein L13
          Length = 146

 Score = 29.6 bits (65), Expect = 5.7
 Identities = 13/31 (41%), Positives = 19/31 (61%)
 Frame = +2

Query: 11  YQKLRENGILISVAKVKQTDHNYHHRLIYLH 103
           +Q L +N I+I+ +KVK T   Y  +L Y H
Sbjct: 53  HQDLGDNVIIINASKVKLTGKKYQQKLYYRH 83
>sp|P00548|KPYK_CHICK Pyruvate kinase, muscle isozyme
          Length = 530

 Score = 29.3 bits (64), Expect = 7.4
 Identities = 10/22 (45%), Positives = 16/22 (72%)
 Frame = -2

Query: 326 DCMENIAWIGRKNIIKTITMGN 261
           +C EN+ W+  KN+IK I +G+
Sbjct: 150 NCDENVLWVDYKNLIKVIDVGS 171
>sp|Q5ZKK1|MARE2_CHICK Microtubule-associated protein RP/EB family member 2
          Length = 338

 Score = 28.9 bits (63), Expect = 9.7
 Identities = 18/53 (33%), Positives = 27/53 (50%)
 Frame = +1

Query: 7   DISKASRKWNFDFSGKSKADRSQLSSPVDLPSPVGYSDRSLPSSSVRKNDSDL 165
           ++ K S   N   +G +K+  S  S P   PS    + ++ PSSS  K+D DL
Sbjct: 190 NLPKKSHHANSPTAGAAKS--SPASKPGSTPSRPSSAKKAAPSSSASKSDKDL 240
>sp|P16453|DCHS_RAT Histidine decarboxylase (HDC)
          Length = 656

 Score = 28.9 bits (63), Expect = 9.7
 Identities = 16/53 (30%), Positives = 26/53 (49%)
 Frame = +1

Query: 109 GYSDRSLPSSSVRKNDSDLMVKRSWDIALGPIKQIPMNLILMWMAGNTISIFP 267
           GY    +PSS+  + DS       WD   G I+QI M  ++ W + +  + +P
Sbjct: 40  GYLRAQIPSSAPEEPDS-------WDSIFGDIEQIIMPGVVHWQSPHMHAYYP 85
>sp|Q9UIS9|MBD1_HUMAN Methyl-CpG-binding domain protein 1 (Methyl-CpG binding protein
           MBD1) (Protein containing methyl-CpG-binding domain 1)
          Length = 605

 Score = 28.9 bits (63), Expect = 9.7
 Identities = 19/70 (27%), Positives = 29/70 (41%)
 Frame = +1

Query: 67  RSQLSSPVDLPSPVGYSDRSLPSSSVRKNDSDLMVKRSWDIALGPIKQIPMNLILMWMAG 246
           R   SSPV +P PV  S  +L   +            SW +AL  +KQ   +    W  G
Sbjct: 460 REGASSPVQVPGPVAASTEALLQEAQCSG-------LSWVVALPQVKQEKADTQDEWTPG 512

Query: 247 NTISIFPIVM 276
             +   P+++
Sbjct: 513 TAVLTSPVLV 522
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 71,024,317
Number of Sequences: 369166
Number of extensions: 1512636
Number of successful extensions: 3852
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 3734
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3845
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4308286840
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)