Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_005_G12
(255 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P40473|YIM2_YEAST Hypothetical 39.5 kDa protein in SIM1-... 32 0.34
sp|Q5NIL6|RBFA_FRATT Ribosome-binding factor A 31 0.77
sp|P48561|TRF5_YEAST Topoisomerase 1-related protein TRF5 31 1.0
sp|P33985|RP542_RHIME RNA polymerase sigma-54 factor 30 1.3
sp|P17263|RP541_RHIME RNA polymerase sigma-54 factor 30 1.3
sp|Q97HJ4|ACPH_CLOAB Acyl carrier protein homolog (ACP) 29 2.9
sp|Q83AQ1|KPRS_COXBU Ribose-phosphate pyrophosphokinase (RP... 29 3.8
sp|Q9UXG7|KTHY1_SULSO Probable thymidylate kinase 1 (dTMP k... 28 5.0
sp|Q8C9S4|CJ118_MOUSE Protein C10orf118 homolog (Oocyte-tes... 28 6.5
sp|P46961|GPI2_YEAST Phosphatidylinositol N-acetylglucosami... 28 6.5
>sp|P40473|YIM2_YEAST Hypothetical 39.5 kDa protein in SIM1-RPI1 intergenic region
Length = 351
Score = 32.3 bits (72), Expect = 0.34
Identities = 17/61 (27%), Positives = 34/61 (55%), Gaps = 1/61 (1%)
Frame = +3
Query: 33 VMIFDKMSKECLEELNKLDKSMSEEIVKLD-ISCNVEYSKIAKQCNKEDNELLDKCLESS 209
+ I ++ + ++ELN D + E+ + + I+ E KI + + N+++DKC+ES
Sbjct: 62 IRILKQLGFDDIQELNACDTGLVEQFLNVRLINDTKELEKIRESNLAKLNQIIDKCMESD 121
Query: 210 K 212
K
Sbjct: 122 K 122
>sp|Q5NIL6|RBFA_FRATT Ribosome-binding factor A
Length = 143
Score = 31.2 bits (69), Expect = 0.77
Identities = 21/58 (36%), Positives = 29/58 (50%), Gaps = 1/58 (1%)
Frame = +3
Query: 42 FDKMSKECLEELNKLDKSMSEEIVKLDISCNVEYSKIAKQCNK-EDNELLDKCLESSK 212
F K+ L K K S I ++D+S ++ Y+KI C ED E +DK E SK
Sbjct: 14 FQKVISLLLRTRIKDAKLASATITEVDLSKDLSYAKIYYTCLAIEDAEYIDKAFEKSK 71
>sp|P48561|TRF5_YEAST Topoisomerase 1-related protein TRF5
Length = 625
Score = 30.8 bits (68), Expect = 1.0
Identities = 13/49 (26%), Positives = 26/49 (53%)
Frame = +3
Query: 45 DKMSKECLEELNKLDKSMSEEIVKLDISCNVEYSKIAKQCNKEDNELLD 191
D+ S L + +++ K S++ + + +CNVEY I C+ + + D
Sbjct: 126 DERSSFLLTDEHEVSKLTSQQSLNTESACNVEYPWIRNHCHSKQRRIAD 174
>sp|P33985|RP542_RHIME RNA polymerase sigma-54 factor
Length = 513
Score = 30.4 bits (67), Expect = 1.3
Identities = 16/62 (25%), Positives = 30/62 (48%), Gaps = 4/62 (6%)
Frame = +3
Query: 39 IFDKMSKECL----EELNKLDKSMSEEIVKLDISCNVEYSKIAKQCNKEDNELLDKCLES 206
+F + ECL N+LD +M + LD+ +++ + K C ++ +L+D E
Sbjct: 219 VFARTLGECLGLQLRARNRLDPAMEALVGNLDLLARRDFASLKKICGVDEEDLIDMFAEI 278
Query: 207 SK 212
K
Sbjct: 279 RK 280
>sp|P17263|RP541_RHIME RNA polymerase sigma-54 factor
Length = 514
Score = 30.4 bits (67), Expect = 1.3
Identities = 15/62 (24%), Positives = 30/62 (48%), Gaps = 4/62 (6%)
Frame = +3
Query: 39 IFDKMSKECL----EELNKLDKSMSEEIVKLDISCNVEYSKIAKQCNKEDNELLDKCLES 206
+F + ECL N+LD +M + L++ +++ + K C ++ +L+D E
Sbjct: 218 VFARTLGECLAIQLRARNRLDPAMEALVANLELLARRDFASLKKICGVDEEDLIDMLAEI 277
Query: 207 SK 212
K
Sbjct: 278 RK 279
>sp|Q97HJ4|ACPH_CLOAB Acyl carrier protein homolog (ACP)
Length = 103
Score = 29.3 bits (64), Expect = 2.9
Identities = 19/84 (22%), Positives = 38/84 (45%), Gaps = 15/84 (17%)
Frame = +3
Query: 3 MNSRTKNRDAVMIFDKMSKECLEELNKLDKSMS---------------EEIVKLDISCNV 137
+ S K +++D S+EC ++N+L+ + S +I+K S N+
Sbjct: 4 LTSEIKKEIRDIVYDFFSEECEVDINELNDNTSVVDDLDGDSLMLIELVDILKKKYSLNI 63
Query: 138 EYSKIAKQCNKEDNELLDKCLESS 209
+ I K K E L+K ++++
Sbjct: 64 QLQSIGKYLLKNPAETLEKVVQTT 87
>sp|Q83AQ1|KPRS_COXBU Ribose-phosphate pyrophosphokinase (RPPK) (Phosphoribosyl
pyrophosphate synthetase) (P-Rib-PP synthetase) (PRPP
synthetase)
Length = 319
Score = 28.9 bits (63), Expect = 3.8
Identities = 17/43 (39%), Positives = 22/43 (51%)
Frame = +3
Query: 66 LEELNKLDKSMSEEIVKLDISCNVEYSKIAKQCNKEDNELLDK 194
LEE LDK + IV D+ V IAK+ N D ++DK
Sbjct: 156 LEEYRLLDKLETPMIVSPDVGGVVRARAIAKRLNDSDLAIIDK 198
>sp|Q9UXG7|KTHY1_SULSO Probable thymidylate kinase 1 (dTMP kinase 1)
Length = 189
Score = 28.5 bits (62), Expect = 5.0
Identities = 22/81 (27%), Positives = 39/81 (48%), Gaps = 7/81 (8%)
Frame = +3
Query: 3 MNSRTKNRDAVMIFDKMSKECLEELNKLDKSMSEEIVKLDISCNVEYSKIAKQCN----- 167
+NS D V++ D + + + K DK EE +K +Y K+AK+ N
Sbjct: 110 VNSYFPKPDMVILLDLPIEVAISRI-KNDKFNFEEKIKSLAKVREKYLKLAKEYNFYVVD 168
Query: 168 --KEDNELLDKCLESSK*NFF 224
K+ NE+L++ ++ + N F
Sbjct: 169 ASKDKNEVLEQAIKIIQKNLF 189
>sp|Q8C9S4|CJ118_MOUSE Protein C10orf118 homolog (Oocyte-testis gene 1 protein)
Length = 917
Score = 28.1 bits (61), Expect = 6.5
Identities = 17/68 (25%), Positives = 33/68 (48%)
Frame = +3
Query: 3 MNSRTKNRDAVMIFDKMSKECLEELNKLDKSMSEEIVKLDISCNVEYSKIAKQCNKEDNE 182
+ RT+ M K + +EELNK K+ +++V D++ +I K+ + ++
Sbjct: 256 LRKRTETEKQHMNTIKQLELRIEELNKEIKASKDQLVAQDVTAKNAIQQIHKEMAQRMDQ 315
Query: 183 LLDKCLES 206
KC E+
Sbjct: 316 ANKKCEEA 323
>sp|P46961|GPI2_YEAST Phosphatidylinositol N-acetylglucosaminyltransferase GPI2 subunit
Length = 280
Score = 28.1 bits (61), Expect = 6.5
Identities = 20/69 (28%), Positives = 33/69 (47%), Gaps = 1/69 (1%)
Frame = -1
Query: 213 ICCFLNICQAIHYLL-YYIVLLFLSIRHCMKYQV*QFLQTSICPIYLILLNILSTFYRIS 37
+ CFL IC ++ +L Y+ + + V FL ++ IY++L+ S FY
Sbjct: 190 VFCFLLICIQLNIILPTYLSVTNKVVPIISNIIVYSFLNVALGWIYMLLIFFASVFYITV 249
Query: 36 LPHLDFWFL 10
LP WF+
Sbjct: 250 LPK---WFI 255
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,792,439
Number of Sequences: 369166
Number of extensions: 347961
Number of successful extensions: 1385
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1364
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1384
length of database: 68,354,980
effective HSP length: 55
effective length of database: 58,194,555
effective search space used: 1687642095
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)