Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_005_G10
(367 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P04636|MDHM_RAT Malate dehydrogenase, mitochondrial prec... 126 2e-29
sp|P00346|MDHM_PIG Malate dehydrogenase, mitochondrial prec... 125 3e-29
sp|P40926|MDHM_HUMAN Malate dehydrogenase, mitochondrial pr... 124 5e-29
sp|P08249|MDHM_MOUSE Malate dehydrogenase, mitochondrial pr... 123 2e-28
sp|Q6D9D1|MDH_ERWCT Malate dehydrogenase 98 7e-21
sp|P37226|MDH_PHOPR Malate dehydrogenase 97 2e-20
sp|Q83Q04|MDH_SHIFL Malate dehydrogenase 96 3e-20
sp|P61889|MDH_ECOLI Malate dehydrogenase >gi|48429005|sp|P6... 94 1e-19
sp|Q5E875|MDH_VIBF1 Malate dehydrogenase 94 1e-19
sp|Q87SU7|MDH_VIBPA Malate dehydrogenase 93 2e-19
>sp|P04636|MDHM_RAT Malate dehydrogenase, mitochondrial precursor
Length = 338
Score = 126 bits (316), Expect = 2e-29
Identities = 59/108 (54%), Positives = 86/108 (79%)
Frame = +3
Query: 6 KIRNAGSEIVEAKAGHGSSTLSMSWAAYRFINSLIEAKSGRTGVIEPAFVKSEETECEYF 185
KI+ AG+E+V+AKAG GS+TLSM++A RF+ SL++A +G+ GVIE +FV+S+ETEC YF
Sbjct: 229 KIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVIECSFVQSKETECTYF 288
Query: 186 ASPLLFGTEGIERNLGIGQMTEFEISLLKSAIPEIKRDIEAGKRFAAN 329
++PLL G +G+E+NLGIG++T FE ++ AIPE+K I+ G+ F N
Sbjct: 289 STPLLLGKKGLEKNLGIGKITPFEEKMIAEAIPELKASIKKGEDFVKN 336
>sp|P00346|MDHM_PIG Malate dehydrogenase, mitochondrial precursor
Length = 338
Score = 125 bits (314), Expect = 3e-29
Identities = 57/108 (52%), Positives = 86/108 (79%)
Frame = +3
Query: 6 KIRNAGSEIVEAKAGHGSSTLSMSWAAYRFINSLIEAKSGRTGVIEPAFVKSEETECEYF 185
+I+ AG+E+V+AKAG GS+TLSM++A RF+ SL++A +G+ GV+E +FVKS+ET+C YF
Sbjct: 229 RIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETDCPYF 288
Query: 186 ASPLLFGTEGIERNLGIGQMTEFEISLLKSAIPEIKRDIEAGKRFAAN 329
++PLL G +GIE+NLGIG+++ FE ++ AIPE+K I+ G+ F N
Sbjct: 289 STPLLLGKKGIEKNLGIGKISPFEEKMIAEAIPELKASIKKGEEFVKN 336
>sp|P40926|MDHM_HUMAN Malate dehydrogenase, mitochondrial precursor
Length = 338
Score = 124 bits (312), Expect = 5e-29
Identities = 57/105 (54%), Positives = 85/105 (80%)
Frame = +3
Query: 6 KIRNAGSEIVEAKAGHGSSTLSMSWAAYRFINSLIEAKSGRTGVIEPAFVKSEETECEYF 185
+I+ AG+E+V+AKAG GS+TLSM++A RF+ SL++A +G+ GV+E +FVKS+ETEC YF
Sbjct: 229 RIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETECTYF 288
Query: 186 ASPLLFGTEGIERNLGIGQMTEFEISLLKSAIPEIKRDIEAGKRF 320
++PLL G +GIE+NLGIG+++ FE ++ AIPE+K I+ G+ F
Sbjct: 289 STPLLLGKKGIEKNLGIGKVSSFEEKMISDAIPELKASIKKGEDF 333
>sp|P08249|MDHM_MOUSE Malate dehydrogenase, mitochondrial precursor
Length = 338
Score = 123 bits (308), Expect = 2e-28
Identities = 57/108 (52%), Positives = 85/108 (78%)
Frame = +3
Query: 6 KIRNAGSEIVEAKAGHGSSTLSMSWAAYRFINSLIEAKSGRTGVIEPAFVKSEETECEYF 185
+I+ AG+E+V+AKAG GS+TLSM++A RF+ SL++A +G GV+E +FV+S+ETEC YF
Sbjct: 229 RIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGLEGVVECSFVQSKETECTYF 288
Query: 186 ASPLLFGTEGIERNLGIGQMTEFEISLLKSAIPEIKRDIEAGKRFAAN 329
++PLL G +G+E+NLGIG++T FE ++ AIPE+K I+ G+ F N
Sbjct: 289 STPLLLGKKGLEKNLGIGKITPFEEKMIAEAIPELKASIKKGEDFVKN 336
>sp|Q6D9D1|MDH_ERWCT Malate dehydrogenase
Length = 311
Score = 97.8 bits (242), Expect = 7e-21
Identities = 49/105 (46%), Positives = 70/105 (66%)
Frame = +3
Query: 6 KIRNAGSEIVEAKAGHGSSTLSMSWAAYRFINSLIEAKSGRTGVIEPAFVKSEETECEYF 185
+I+NAG+E+VEAKAG GS+TLSM AA RF SL+ A G GV+E A+V+S+ +F
Sbjct: 205 RIQNAGTEVVEAKAGGGSATLSMGQAAARFGLSLVRALQGENGVVECAYVESDGKHARFF 264
Query: 186 ASPLLFGTEGIERNLGIGQMTEFEISLLKSAIPEIKRDIEAGKRF 320
A P+L G +G+ IG ++ FE + L S + +K+DIE G+ F
Sbjct: 265 AQPILLGKDGVAERKDIGTLSAFEQNALSSMLDTLKQDIELGETF 309
>sp|P37226|MDH_PHOPR Malate dehydrogenase
Length = 312
Score = 96.7 bits (239), Expect = 2e-20
Identities = 47/107 (43%), Positives = 70/107 (65%)
Frame = +3
Query: 6 KIRNAGSEIVEAKAGHGSSTLSMSWAAYRFINSLIEAKSGRTGVIEPAFVKSEETECEYF 185
+I+NAG+E+VEAKAG GS+TLSM AAYRF SL+ A G G++E A+V+ + +F
Sbjct: 205 RIQNAGTEVVEAKAGGGSATLSMGQAAYRFGLSLVRALQGEQGIVECAYVEGDGKHARFF 264
Query: 186 ASPLLFGTEGIERNLGIGQMTEFEISLLKSAIPEIKRDIEAGKRFAA 326
A P+L G +G+E + G+++ FE L + + + DI G+ FAA
Sbjct: 265 AQPVLLGKDGVEEVIDYGKLSTFEQEALNNMLDTLTSDITLGEEFAA 311
>sp|Q83Q04|MDH_SHIFL Malate dehydrogenase
Length = 312
Score = 95.5 bits (236), Expect = 3e-20
Identities = 48/105 (45%), Positives = 68/105 (64%)
Frame = +3
Query: 6 KIRNAGSEIVEAKAGHGSSTLSMSWAAYRFINSLIEAKSGRTGVIEPAFVKSEETECEYF 185
+I+NAG+E+VEAKAG GS+TLSM AA RF SL+ A G GV+E A+V+ + +F
Sbjct: 205 RIQNAGTEVVEAKAGGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFF 264
Query: 186 ASPLLFGTEGIERNLGIGQMTEFEISLLKSAIPEIKRDIEAGKRF 320
+ PLL G G+E IG ++ FE + L+ + +K+DI GK F
Sbjct: 265 SQPLLLGKNGVEERKSIGTLSAFEQNALEGMLDTLKKDIALGKEF 309
>sp|P61889|MDH_ECOLI Malate dehydrogenase
sp|P61891|MDH_ECO57 Malate dehydrogenase
sp|P61890|MDH_ECOL6 Malate dehydrogenase
Length = 312
Score = 94.0 bits (232), Expect = 1e-19
Identities = 47/105 (44%), Positives = 68/105 (64%)
Frame = +3
Query: 6 KIRNAGSEIVEAKAGHGSSTLSMSWAAYRFINSLIEAKSGRTGVIEPAFVKSEETECEYF 185
+I+NAG+E+VEAKAG GS+TLSM AA RF SL+ A G GV+E A+V+ + +F
Sbjct: 205 RIQNAGTEVVEAKAGGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFF 264
Query: 186 ASPLLFGTEGIERNLGIGQMTEFEISLLKSAIPEIKRDIEAGKRF 320
+ PLL G G+E IG ++ FE + L+ + +K+DI G+ F
Sbjct: 265 SQPLLLGKNGVEERKSIGTLSAFEQNALEGMLDTLKKDIALGEEF 309
>sp|Q5E875|MDH_VIBF1 Malate dehydrogenase
Length = 311
Score = 93.6 bits (231), Expect = 1e-19
Identities = 47/105 (44%), Positives = 69/105 (65%)
Frame = +3
Query: 6 KIRNAGSEIVEAKAGHGSSTLSMSWAAYRFINSLIEAKSGRTGVIEPAFVKSEETECEYF 185
+I+NAG+E+VEAKAG GS+TLSM AA RF SL++A SG GV+E A+V+ +F
Sbjct: 205 RIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVKALSGEEGVVECAYVEGNGEHARFF 264
Query: 186 ASPLLFGTEGIERNLGIGQMTEFEISLLKSAIPEIKRDIEAGKRF 320
A P+L G G+E G+++ FE L+S + ++ DI+ G+ F
Sbjct: 265 AQPILLGKNGVEEIQSYGELSAFEQEALESMLDTLRGDIKIGEEF 309
>sp|Q87SU7|MDH_VIBPA Malate dehydrogenase
Length = 311
Score = 93.2 bits (230), Expect = 2e-19
Identities = 47/106 (44%), Positives = 67/106 (63%)
Frame = +3
Query: 6 KIRNAGSEIVEAKAGHGSSTLSMSWAAYRFINSLIEAKSGRTGVIEPAFVKSEETECEYF 185
+I+NAG+E+VEAKAG GS+TLSM AA RF +L+ A G GV+E A+V+ + YF
Sbjct: 205 RIQNAGTEVVEAKAGGGSATLSMGQAACRFGLALVRALQGEEGVVECAYVEGDSEHAPYF 264
Query: 186 ASPLLFGTEGIERNLGIGQMTEFEISLLKSAIPEIKRDIEAGKRFA 323
A P+ G EG+E L G++++FE + L + + DI G FA
Sbjct: 265 AQPVKLGKEGVEEVLSYGELSDFEKAALDGMLETLNGDINIGVEFA 310
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39,406,976
Number of Sequences: 369166
Number of extensions: 652080
Number of successful extensions: 1920
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1878
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1907
length of database: 68,354,980
effective HSP length: 88
effective length of database: 52,098,300
effective search space used: 1719243900
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)