Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_005_G06
(912 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q62415|ASPP1_MOUSE Apoptosis stimulating of p53 protein ... 169 8e-42
sp|Q96KQ4|ASPP1_HUMAN Apoptosis stimulating of p53 protein ... 169 1e-41
sp|Q8CG79|ASPP2_MOUSE Apoptosis stimulating of p53 protein ... 163 5e-40
sp|Q9XVN3|IASPP_CAEEL Apoptotic enhancer 1 protein (Inhibit... 163 7e-40
sp|Q13625|ASPP2_HUMAN Apoptosis stimulating of p53 protein ... 162 1e-39
sp|Q8WUF5|IASPP_HUMAN RelA-associated inhibitor (Inhibitor ... 137 4e-32
sp|Q5I1X5|IASPP_MOUSE RelA-associated inhibitor (Inhibitor ... 132 1e-30
sp|Q96T49|PP16B_HUMAN Protein phosphatase 1 regulatory inhi... 80 8e-15
sp|Q8VHQ3|PP16B_MOUSE Protein phosphatase 1 regulatory inhi... 80 8e-15
sp|Q95N27|PP16B_BOVIN Protein phosphatase 1 regulatory inhi... 80 8e-15
>sp|Q62415|ASPP1_MOUSE Apoptosis stimulating of p53 protein 1 (Protein phosphatase 1
regulatory subunit 13B)
Length = 1087
Score = 169 bits (429), Expect = 8e-42
Identities = 105/302 (34%), Positives = 158/302 (52%), Gaps = 9/302 (2%)
Frame = +2
Query: 29 PEDLVDIQPSTDQAGQVEETAFIQPIDDVNSSSENDINIQDVSD--ADSPIWSSSVKRAI 202
PE+L+ Q + A +P +D N++ + + + A++P V A
Sbjct: 799 PEELICPQTTHQTA---------EPTEDNNNNVAPVPSTEQIPSPVAEAPSEEDQVPPAP 849
Query: 203 EALNNEPTKRILKKTVSQSSPNPAKRNAH-----FHPHSXXXXXXXXXXXXXVKEMSAKV 367
+ P K + PN ++R H F+P + V+ + +V
Sbjct: 850 LSPVIHPPAASASKRTNLKKPN-SERTGHGLRVRFNPLALLLDASLEGEFDLVQRIIYEV 908
Query: 368 EDISEATDEGITALHNAVCSGREDIVKFLLDLGAHVNAGDTDGWTPLHCAASCNNVGMAR 547
ED S+ DEGIT LHNAVC+G IVKFLLD G +VNA D+DGWTPLHCAASCN+V + +
Sbjct: 909 EDPSKPNDEGITPLHNAVCAGHHHIVKFLLDFGVNVNAADSDGWTPLHCAASCNSVHLCK 968
Query: 548 LLVEYGASLFATSISDSEIPLQKCEVDDD--VECEQYLFNQQDLLGVVNDSKVYALFHYE 721
LVE GA++FA++ISD E KCE ++ ++C Q+L+ Q+ LGV+N VYAL+ YE
Sbjct: 969 QLVESGAAIFASTISDIETAADKCEEMEEGYIQCSQFLYGVQEKLGVMNKGTVYALWDYE 1028
Query: 722 PNHEDEFSIYPNQELKIIDKEPQGEPGWFLAAETFDSTKVGLVPKAYVSCYRLVLPNKAN 901
+ DE S + + I+ ++ + E W+ A + G VPK + Y + P +
Sbjct: 1029 AQNSDELSFHEGDAITILRRKDENETEWWWARL---GDREGYVPKNLLGLYPRIKPRQRT 1085
Query: 902 TA 907
A
Sbjct: 1086 LA 1087
>sp|Q96KQ4|ASPP1_HUMAN Apoptosis stimulating of p53 protein 1 (Protein phosphatase 1
regulatory subunit 13B)
Length = 1090
Score = 169 bits (427), Expect = 1e-41
Identities = 98/259 (37%), Positives = 143/259 (55%), Gaps = 7/259 (2%)
Frame = +2
Query: 152 VSDADSPIWSSSVKRAIEALNNEPTKRILKKTVSQSSPNPAKRNAH-----FHPHSXXXX 316
V++A SP + ++ P K+T + PN ++R H F+P +
Sbjct: 837 VAEAPSPGEEQVPPAPLPPASHPPATSTNKRT-NLKKPN-SERTGHGLRVRFNPLALLLD 894
Query: 317 XXXXXXXXXVKEMSAKVEDISEATDEGITALHNAVCSGREDIVKFLLDLGAHVNAGDTDG 496
V+ + +VED S+ DEGIT LHNAVC+G IVKFLLD G +VNA D+DG
Sbjct: 895 ASLEGEFDLVQRIIYEVEDPSKPNDEGITPLHNAVCAGHHHIVKFLLDFGVNVNAADSDG 954
Query: 497 WTPLHCAASCNNVGMARLLVEYGASLFATSISDSEIPLQKCEVDDD--VECEQYLFNQQD 670
WTPLHCAASCN+V + + LVE GA++FA++ISD E KCE ++ ++C Q+L+ Q+
Sbjct: 955 WTPLHCAASCNSVHLCKQLVESGAAIFASTISDIETAADKCEEMEEGYIQCSQFLYGVQE 1014
Query: 671 LLGVVNDSKVYALFHYEPNHEDEFSIYPNQELKIIDKEPQGEPGWFLAAETFDSTKVGLV 850
LGV+N YAL+ YE + DE S + L I+ ++ + E W+ A + G V
Sbjct: 1015 KLGVMNKGVAYALWDYEAQNSDELSFHEGDALTILRRKDESETEWWWARL---GDREGYV 1071
Query: 851 PKAYVSCYRLVLPNKANTA 907
PK + Y + P + A
Sbjct: 1072 PKNLLGLYPRIKPRQRTLA 1090
>sp|Q8CG79|ASPP2_MOUSE Apoptosis stimulating of p53 protein 2 (Tumor suppressor p53-binding
protein 2)
Length = 1088
Score = 163 bits (413), Expect = 5e-40
Identities = 90/232 (38%), Positives = 135/232 (58%), Gaps = 3/232 (1%)
Frame = +2
Query: 221 PTKRI-LKKTVSQSSPNPAKRNAHFHPHSXXXXXXXXXXXXXVKEMSAKVEDISEATDEG 397
P KR L+KT S+ + + F+P + V+ + +V+D S DEG
Sbjct: 862 PGKRTNLRKTGSERIAHGMR--VKFNPLALLLDSSLEGEFDLVQRIIYEVDDPSLPNDEG 919
Query: 398 ITALHNAVCSGREDIVKFLLDLGAHVNAGDTDGWTPLHCAASCNNVGMARLLVEYGASLF 577
ITALHNAVC+G +IVKFL+ G +VNA D+DGWTPLHCAASCNNV + + LVE GA++F
Sbjct: 920 ITALHNAVCAGHTEIVKFLVQFGVNVNAADSDGWTPLHCAASCNNVQVCKFLVESGAAVF 979
Query: 578 ATSISDSEIPLQKCEVDDD--VECEQYLFNQQDLLGVVNDSKVYALFHYEPNHEDEFSIY 751
A + SD + KCE ++ +C Q+L+ Q+ +G++N +YAL+ YEP H+DE +
Sbjct: 980 AMTYSDMQTAADKCEEMEEGYTQCSQFLYGVQEKMGIMNKGVIYALWDYEPQHDDELLMK 1039
Query: 752 PNQELKIIDKEPQGEPGWFLAAETFDSTKVGLVPKAYVSCYRLVLPNKANTA 907
+ +I +E + E W+ A + K G VP+ + Y + P + + A
Sbjct: 1040 EGDCMTVIRREDEEEIEWWWARL---NDKEGYVPRNLLGLYPRIKPRQRSLA 1088
>sp|Q9XVN3|IASPP_CAEEL Apoptotic enhancer 1 protein (Inhibitor member of the ASPP family)
(Protein iASPP) (Ce-iASPP)
Length = 769
Score = 163 bits (412), Expect = 7e-40
Identities = 88/218 (40%), Positives = 127/218 (58%), Gaps = 2/218 (0%)
Frame = +2
Query: 227 KRILKKTVSQSSPNPAKRNAHFHPHSXXXXXXXXXXXXXVKEMSAKVEDISEATDEGITA 406
+R +K + + + K F P + V+ ++K+ D+S+A DEGITA
Sbjct: 530 RRTVKGILRRPNEKMNKGRIEFDPLALLLDAALEGELDLVRSSASKLTDVSQANDEGITA 589
Query: 407 LHNAVCSGREDIVKFLLDLGAHVNAGDTDGWTPLHCAASCNNVGMARLLVEYGASLFATS 586
LHNA+C+G +IV+FL++ A VNA D+DGWTPLHCAASCNN+ M R LVE G + A++
Sbjct: 590 LHNAICAGHYEIVRFLIENDADVNAQDSDGWTPLHCAASCNNLPMVRQLVEGGGCVLAST 649
Query: 587 ISDSEIPLQKCEVDDD--VECEQYLFNQQDLLGVVNDSKVYALFHYEPNHEDEFSIYPNQ 760
+SD E P++KCE D+D C +YL + G +N KVYA + YE EDE S
Sbjct: 650 LSDMETPVEKCEEDEDGYDGCLKYLSAAHNSTGSINTGKVYAAYGYEAAFEDELSFDAGD 709
Query: 761 ELKIIDKEPQGEPGWFLAAETFDSTKVGLVPKAYVSCY 874
EL +I+K+ + W E + K G VP+ Y++ Y
Sbjct: 710 ELTVIEKDKVDKNWW--TCEKNNGEK-GQVPRTYLALY 744
>sp|Q13625|ASPP2_HUMAN Apoptosis stimulating of p53 protein 2 (Tumor suppressor p53-binding
protein 2) (p53-binding protein 2) (53BP2) (Bcl2-binding
protein) (Bbp)
Length = 1128
Score = 162 bits (410), Expect = 1e-39
Identities = 90/232 (38%), Positives = 135/232 (58%), Gaps = 3/232 (1%)
Frame = +2
Query: 221 PTKRI-LKKTVSQSSPNPAKRNAHFHPHSXXXXXXXXXXXXXVKEMSAKVEDISEATDEG 397
P KR L+KT S+ + + F+P + V+ + +V+D S DEG
Sbjct: 902 PGKRTNLRKTGSERIAHGMR--VKFNPLALLLDSSLEGEFDLVQRIIYEVDDPSLPNDEG 959
Query: 398 ITALHNAVCSGREDIVKFLLDLGAHVNAGDTDGWTPLHCAASCNNVGMARLLVEYGASLF 577
ITALHNAVC+G +IVKFL+ G +VNA D+DGWTPLHCAASCNNV + + LVE GA++F
Sbjct: 960 ITALHNAVCAGHTEIVKFLVQFGVNVNAADSDGWTPLHCAASCNNVQVCKFLVESGAAVF 1019
Query: 578 ATSISDSEIPLQKCEVDDD--VECEQYLFNQQDLLGVVNDSKVYALFHYEPNHEDEFSIY 751
A + SD + KCE ++ +C Q+L+ Q+ +G++N +YAL+ YEP ++DE +
Sbjct: 1020 AMTYSDMQTAADKCEEMEEGYTQCSQFLYGVQEKMGIMNKGVIYALWDYEPQNDDELPMK 1079
Query: 752 PNQELKIIDKEPQGEPGWFLAAETFDSTKVGLVPKAYVSCYRLVLPNKANTA 907
+ II +E + E W+ A + K G VP+ + Y + P + + A
Sbjct: 1080 EGDCMTIIHREDEDEIEWWWARL---NDKEGYVPRNLLGLYPRIKPRQRSLA 1128
>sp|Q8WUF5|IASPP_HUMAN RelA-associated inhibitor (Inhibitor of ASPP protein) (Protein iASPP)
(PPP1R13B-like protein) (NFkB-interacting protein 1)
Length = 828
Score = 137 bits (345), Expect = 4e-32
Identities = 74/222 (33%), Positives = 121/222 (54%), Gaps = 3/222 (1%)
Frame = +2
Query: 242 KTVSQSSPNPAK-RNAHFHPHSXXXXXXXXXXXXXVKEMSAKVEDISEATDEGITALHNA 418
++V + + +P K R A +P V++ ++ D S+ +EGITALHNA
Sbjct: 608 RSVLRKAGSPRKARRARLNPLVLLLDAALTGELEVVQQAVKEMNDPSQPNEEGITALHNA 667
Query: 419 VCSGREDIVKFLLDLGAHVNAGDTDGWTPLHCAASCNNVGMARLLVEYGASLFATSISDS 598
+C IV FL+ GA+VN+ D+ GWTPLHCAASCN+ + LV++GA++FAT++SD
Sbjct: 668 ICGANYSIVDFLITAGANVNSPDSHGWTPLHCAASCNDTVICMALVQHGAAIFATTLSDG 727
Query: 599 EIPLQKCEV--DDDVECEQYLFNQQDLLGVVNDSKVYALFHYEPNHEDEFSIYPNQELKI 772
+KC+ + +C YL + + +G++N VYAL+ Y DE S + + +
Sbjct: 728 ATAFEKCDPYREGYADCATYLADVEQSMGLMNSGAVYALWDYSAEFGDELSFREGESVTV 787
Query: 773 IDKEPQGEPGWFLAAETFDSTKVGLVPKAYVSCYRLVLPNKA 898
+ ++ E W+ AA + G VP+ Y + V P ++
Sbjct: 788 LRRDGPEETDWWWAAL---HGQEGYVPRNYFGLFPRVKPQRS 826
>sp|Q5I1X5|IASPP_MOUSE RelA-associated inhibitor (Inhibitor of ASPP protein) (Protein iASPP)
(PPP1R13B-like protein) (NFkB-interacting protein 1)
Length = 824
Score = 132 bits (332), Expect = 1e-30
Identities = 72/218 (33%), Positives = 120/218 (55%), Gaps = 2/218 (0%)
Frame = +2
Query: 227 KRILKKTVSQSSPNPAKRNAHFHPHSXXXXXXXXXXXXXVKEMSAKVEDISEATDEGITA 406
+ +L+K SP A+R A +P V++ ++ D S+ +EGITA
Sbjct: 604 RSVLRKV---GSPRKARR-ARLNPLVLLLDAALTGELDVVQQAVKEMNDPSQPNEEGITA 659
Query: 407 LHNAVCSGREDIVKFLLDLGAHVNAGDTDGWTPLHCAASCNNVGMARLLVEYGASLFATS 586
LHNA+C IV FL+ GA+VN+ D+ GWTPLHCAASCN+ + LV++GA++FAT+
Sbjct: 660 LHNAICGANYPIVDFLIAAGANVNSPDSHGWTPLHCAASCNDTAICTALVQHGAAIFATT 719
Query: 587 ISDSEIPLQKCEV--DDDVECEQYLFNQQDLLGVVNDSKVYALFHYEPNHEDEFSIYPNQ 760
+SD ++KC+ + +C YL + + +G++++ VYAL+ Y DE S +
Sbjct: 720 LSDGATAIEKCDPYREGYADCATYLADVEQSMGLMHNGVVYALWDYSAEFGDELSFREGE 779
Query: 761 ELKIIDKEPQGEPGWFLAAETFDSTKVGLVPKAYVSCY 874
+ ++ ++ E W+ A+ + G VP+ Y +
Sbjct: 780 SVTVLRRDGPEETDWWWASL---HGQEGYVPRNYFGLF 814
>sp|Q96T49|PP16B_HUMAN Protein phosphatase 1 regulatory inhibitor subunit 16B
(TGF-beta-inhibited membrane-associated protein)
(hTIMAP) (CAAX box protein TIMAP) (Ankyrin repeat domain
protein 4)
Length = 567
Score = 80.1 bits (196), Expect = 8e-15
Identities = 37/78 (47%), Positives = 52/78 (66%)
Frame = +2
Query: 389 DEGITALHNAVCSGREDIVKFLLDLGAHVNAGDTDGWTPLHCAASCNNVGMARLLVEYGA 568
++G+TALH E+IVK LL GA+VNA D + WTPLH AA+C ++ + ++LV+YGA
Sbjct: 99 EDGLTALHQCCIDNFEEIVKLLLSHGANVNAKDNELWTPLHAAATCGHINLVKILVQYGA 158
Query: 569 SLFATSISDSEIPLQKCE 622
L A + SD +P CE
Sbjct: 159 DLLAVN-SDGNMPYDLCE 175
Score = 65.5 bits (158), Expect = 2e-10
Identities = 38/93 (40%), Positives = 51/93 (54%)
Frame = +2
Query: 353 MSAKVEDISEATDEGITALHNAVCSGREDIVKFLLDLGAHVNAGDTDGWTPLHCAASCNN 532
M A +D+ +G T LH A +G + LLD G V+ D DGW PLH AA
Sbjct: 215 MIAAGQDLDWIDAQGATLLHIAGANGYLRAAELLLDHGVRVDVKDWDGWEPLHAAAFWGQ 274
Query: 533 VGMARLLVEYGASLFATSISDSEIPLQKCEVDD 631
+ MA LLV +GASL A + D E+P+ CE ++
Sbjct: 275 MQMAELLVSHGASLSARTSMD-EMPIDLCEEEE 306
>sp|Q8VHQ3|PP16B_MOUSE Protein phosphatase 1 regulatory inhibitor subunit 16B
(TGF-beta-inhibited membrane-associated protein) (CAAX
box protein TIMAP)
Length = 568
Score = 80.1 bits (196), Expect = 8e-15
Identities = 37/78 (47%), Positives = 52/78 (66%)
Frame = +2
Query: 389 DEGITALHNAVCSGREDIVKFLLDLGAHVNAGDTDGWTPLHCAASCNNVGMARLLVEYGA 568
++G+TALH E+IVK LL GA+VNA D + WTPLH AA+C ++ + ++LV+YGA
Sbjct: 99 EDGLTALHQCCIDNFEEIVKLLLSHGANVNAKDNELWTPLHAAATCGHINLVKILVQYGA 158
Query: 569 SLFATSISDSEIPLQKCE 622
L A + SD +P CE
Sbjct: 159 DLLAVN-SDGNMPYDLCE 175
Score = 65.9 bits (159), Expect = 2e-10
Identities = 38/93 (40%), Positives = 51/93 (54%)
Frame = +2
Query: 353 MSAKVEDISEATDEGITALHNAVCSGREDIVKFLLDLGAHVNAGDTDGWTPLHCAASCNN 532
M A +D+ +G T LH A +G + LLD G V+ D DGW PLH AA
Sbjct: 215 MIAAGQDLDWIDGQGATLLHIAGANGYLRAAELLLDHGVRVDVKDWDGWEPLHAAAFWGQ 274
Query: 533 VGMARLLVEYGASLFATSISDSEIPLQKCEVDD 631
+ MA LLV +GASL A + D E+P+ CE ++
Sbjct: 275 MPMAELLVSHGASLSARTSMD-EMPIDLCEEEE 306
>sp|Q95N27|PP16B_BOVIN Protein phosphatase 1 regulatory inhibitor subunit 16B
(TGF-beta-inhibited membrane-associated protein)
(bTIMAP) (CAAX box protein TIMAP)
Length = 568
Score = 80.1 bits (196), Expect = 8e-15
Identities = 37/78 (47%), Positives = 52/78 (66%)
Frame = +2
Query: 389 DEGITALHNAVCSGREDIVKFLLDLGAHVNAGDTDGWTPLHCAASCNNVGMARLLVEYGA 568
++G+TALH E+IVK LL GA+VNA D + WTPLH AA+C ++ + ++LV+YGA
Sbjct: 99 EDGLTALHQCCIDNFEEIVKLLLSHGANVNAKDNELWTPLHAAATCGHINLVKILVQYGA 158
Query: 569 SLFATSISDSEIPLQKCE 622
L A + SD +P CE
Sbjct: 159 DLLAVN-SDGNMPYDLCE 175
Score = 65.9 bits (159), Expect = 2e-10
Identities = 38/93 (40%), Positives = 51/93 (54%)
Frame = +2
Query: 353 MSAKVEDISEATDEGITALHNAVCSGREDIVKFLLDLGAHVNAGDTDGWTPLHCAASCNN 532
M A +D+ +G T LH A +G + LLD G V+ D DGW PLH AA
Sbjct: 215 MIAAGQDLDWVDAQGATLLHIAGANGYLRAAELLLDHGVRVDVKDWDGWEPLHAAAFWGQ 274
Query: 533 VGMARLLVEYGASLFATSISDSEIPLQKCEVDD 631
+ MA LLV +GASL A + D E+P+ CE ++
Sbjct: 275 MQMAELLVSHGASLSARTSMD-EMPIDLCEEEE 306
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 106,386,882
Number of Sequences: 369166
Number of extensions: 2192676
Number of successful extensions: 8522
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 7353
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8407
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9270587090
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)