Planarian EST Database


Dr_sW_005_F20

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_005_F20
         (477 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q6P2C8|CRSP8_HUMAN  CRSP complex subunit 8 (Cofactor requ...    48   1e-05
sp|P56765|ACCD_ARATH  Acetyl-coenzyme A carboxylase carboxyl...    32   0.75 
sp|P76322|YEDM_ECOLI  Hypothetical protein yedM                    32   0.75 
sp|P10686|PLCG1_RAT  1-phosphatidylinositol-4,5-bisphosphate...    31   1.7  
sp|P08487|PLCG1_BOVIN  1-phosphatidylinositol-4,5-bisphospha...    31   1.7  
sp|P34427|LIN36_CAEEL  Abnormal cell lineage protein 36 (Pro...    30   2.2  
sp|P49290|PERE_MOUSE  Eosinophil peroxidase precursor (EPO) ...    29   4.9  
sp|P37709|TRHY_RABIT  Trichohyalin                                 29   4.9  
sp|Q8HXG3|RHPN2_CANFA  Rhophilin-2 (GTP-Rho binding protein ...    29   4.9  
sp|P98158|LRP2_RAT  Low-density lipoprotein receptor-related...    29   6.4  
>sp|Q6P2C8|CRSP8_HUMAN CRSP complex subunit 8 (Cofactor required for Sp1 transcriptional
           activation subunit 8) (Transcriptional coactivator
           CRSP34) (P37 TRAP/SMCC/PC2 subunit)
          Length = 311

 Score = 48.1 bits (113), Expect = 1e-05
 Identities = 36/136 (26%), Positives = 61/136 (44%)
 Frame = +2

Query: 44  ARILVKVDETLTCLLRFEGVDLSLVLVKGPMESFNENIACGPEQSADVKNKIDYFTPSKM 223
           A +LV + + L  ++    + +   +VKG    +NEN+            K+D ++ S  
Sbjct: 188 AMLLVTLGKVLKVIVVMRSLFIDRTIVKG----YNENVYTED-------GKLDIWSKSNY 236

Query: 224 RLFREITNEAIVLLSSLRSNYFSPFDQHQKFFEWLRSFSNLHSQPCSVCNRILGKNHLPP 403
           ++F+++T+ A   L   +     P    + F  WLRS+  L   PC  C + L ++ LPP
Sbjct: 237 QVFQKVTDHATTALLHYQLPQM-PDVVVRSFMTWLRSYIKLFQAPCQRCGKFL-QDGLPP 294

Query: 404 FRRDLADFCRVLHDNC 451
             RD        HD C
Sbjct: 295 TWRDFRTL-EAFHDTC 309
>sp|P56765|ACCD_ARATH Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta
           (ACCase beta chain)
          Length = 488

 Score = 32.0 bits (71), Expect = 0.75
 Identities = 29/101 (28%), Positives = 46/101 (45%), Gaps = 9/101 (8%)
 Frame = +2

Query: 176 SADVKNKIDYFTPSK-MRLFREITNEAIVLLSSLRSNYFSPFDQHQKFFEWLRSFSNLHS 352
           S+   N +D    SK +R F  I+++   +  S +++Y   FD+ +K FE    FS+L  
Sbjct: 59  SSSYSNNVDLLVSSKDIRNF--ISDDTFFVRDSNKNSYSIFFDKKKKIFEIDNDFSDLEK 116

Query: 353 QPCSVC------NRILGKN--HLPPFRRDLADFCRVLHDNC 451
              S C      NR  G N  H  P+ +D    C    ++C
Sbjct: 117 FFYSYCSSSYLNNRSKGDNDLHYDPYIKDTKYNCTNHINSC 157
>sp|P76322|YEDM_ECOLI Hypothetical protein yedM
          Length = 116

 Score = 32.0 bits (71), Expect = 0.75
 Identities = 18/61 (29%), Positives = 31/61 (50%), Gaps = 6/61 (9%)
 Frame = +2

Query: 191 NKIDYFTPSKMRLFREITNEAIV------LLSSLRSNYFSPFDQHQKFFEWLRSFSNLHS 352
           NK+   T S ++    ++++AI+      L+S L +  F+P  +  K F  L  F  LHS
Sbjct: 17  NKVTSNTESTIKQHELVSDDAIINELSSELVSCLGNGKFTPISEDSKLFNMLSEFKLLHS 76

Query: 353 Q 355
           +
Sbjct: 77  E 77
>sp|P10686|PLCG1_RAT 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1
            (Phosphoinositide phospholipase C) (PLC-gamma-1)
            (Phospholipase C-gamma-1) (PLC-II) (PLC-148)
          Length = 1290

 Score = 30.8 bits (68), Expect = 1.7
 Identities = 16/45 (35%), Positives = 22/45 (48%)
 Frame = +2

Query: 266  SSLRSNYFSPFDQHQKFFEWLRSFSNLHSQPCSVCNRILGKNHLP 400
            S++R   F PFD+       LR       +PC +C  +LG  HLP
Sbjct: 1067 STMRDEAFDPFDKSS-----LRGL-----EPCVICIEVLGARHLP 1101
>sp|P08487|PLCG1_BOVIN 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1
            (Phosphoinositide phospholipase C) (PLC-gamma-1)
            (Phospholipase C-gamma-1) (PLC-II) (PLC-148)
          Length = 1291

 Score = 30.8 bits (68), Expect = 1.7
 Identities = 16/45 (35%), Positives = 22/45 (48%)
 Frame = +2

Query: 266  SSLRSNYFSPFDQHQKFFEWLRSFSNLHSQPCSVCNRILGKNHLP 400
            S +R   F PFD+       LR       +PC++C  +LG  HLP
Sbjct: 1067 SVMRDEAFDPFDKSS-----LRGL-----EPCAICIEVLGARHLP 1101
>sp|P34427|LIN36_CAEEL Abnormal cell lineage protein 36 (Protein lin-36)
          Length = 962

 Score = 30.4 bits (67), Expect = 2.2
 Identities = 13/29 (44%), Positives = 20/29 (68%), Gaps = 2/29 (6%)
 Frame = +2

Query: 317 FEWLRSFSNLHSQPCSVCNRIL--GKNHL 397
           F+    +SNL  +PC+VCNR++  G+ HL
Sbjct: 152 FKKFSPYSNLTHKPCTVCNRVMKSGEMHL 180
>sp|P49290|PERE_MOUSE Eosinophil peroxidase precursor (EPO) [Contains: Eosinophil
           peroxidase light chain; Eosinophil peroxidase heavy
           chain]
          Length = 716

 Score = 29.3 bits (64), Expect = 4.9
 Identities = 25/84 (29%), Positives = 39/84 (46%), Gaps = 6/84 (7%)
 Frame = +2

Query: 143 FNENIACGPEQSADVKNKIDY---FTPSKMRLFREITNEAIVLLSSLRSNYFSPFDQHQK 313
           F    AC P+    V+N+I+    F  + M    E+T   + L    R+N+      +Q+
Sbjct: 286 FRSAPAC-PQNRNKVRNQINALTSFVDASMVYGSEVT---LALRLRNRTNFLGLLATNQR 341

Query: 314 FFEWLRS---FSNLHSQPCSVCNR 376
           F +  R+   F NLH  PC + NR
Sbjct: 342 FQDNGRALLPFDNLHEDPCLLTNR 365
>sp|P37709|TRHY_RABIT Trichohyalin
          Length = 1407

 Score = 29.3 bits (64), Expect = 4.9
 Identities = 22/83 (26%), Positives = 38/83 (45%)
 Frame = +1

Query: 145  QRKHRLRARAKRGCQK*NRLFHSEQNAIVQRNHERSNRFIVELAIELFLSVRSASEILRM 324
            +++ R + R ++  ++ +R F  E+  + +R  ER  R       E    +R   ++LR 
Sbjct: 1080 EQQRRRQEREQQLRRERDRKFREEEQLLQEREEERLRRQ------ERARKLREEEQLLRR 1133

Query: 325  AAQLLESPLAAVFRVQSNLGQES 393
              QLL       FR +  L QES
Sbjct: 1134 EEQLLRQERDRKFREEEQLLQES 1156
>sp|Q8HXG3|RHPN2_CANFA Rhophilin-2 (GTP-Rho binding protein 2) (76 kDa RhoB effector
           protein) (p76RBE)
          Length = 686

 Score = 29.3 bits (64), Expect = 4.9
 Identities = 25/97 (25%), Positives = 44/97 (45%), Gaps = 1/97 (1%)
 Frame = +2

Query: 101 VDLSLVLVKGPMESFNENIACGPEQSADVKN-KIDYFTPSKMRLFREITNEAIVLLSSLR 277
           +D S+VL    +E ++E+     ++ AD+ + +    TPS+     E+     + L  + 
Sbjct: 123 IDFSVVLKDFILEHYSEDSYLYEDEIADLMDLRQACRTPSRDEAGVELLMSYFIQLGFVE 182

Query: 278 SNYFSPFDQHQKFFEWLRSFSNLHSQPCSVCNRILGK 388
           S +F P  Q    F W   + +L   P S  N +L K
Sbjct: 183 SRFFPPTRQMGILFTW---YDSLTGVPVSQQNLLLEK 216
>sp|P98158|LRP2_RAT Low-density lipoprotein receptor-related protein 2 precursor
            (Megalin) (Glycoprotein 330) (gp330)
          Length = 4660

 Score = 28.9 bits (63), Expect = 6.4
 Identities = 12/45 (26%), Positives = 23/45 (51%)
 Frame = +2

Query: 242  TNEAIVLLSSLRSNYFSPFDQHQKFFEWLRSFSNLHSQPCSVCNR 376
            +N+A+V +S ++  Y   FD  ++F  W+ +   +H       NR
Sbjct: 1768 SNDAMVPISGIQHGYDVEFDDSEQFIYWVENPGEIHRVKTDGSNR 1812
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57,262,790
Number of Sequences: 369166
Number of extensions: 1096558
Number of successful extensions: 3922
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3803
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3922
length of database: 68,354,980
effective HSP length: 101
effective length of database: 49,696,745
effective search space used: 2832714465
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)