Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_005_F13
(975 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q13283|G3BP_HUMAN Ras-GTPase-activating protein binding ... 124 4e-28
sp|Q9UN86|G3B2_HUMAN Ras-GTPase-activating protein binding ... 119 2e-26
sp|P97379|G3B2_MOUSE Ras-GTPase-activating protein binding ... 116 1e-25
sp|P97855|G3BP_MOUSE Ras-GTPase-activating protein binding ... 112 1e-24
sp|O94260|G3BP_SCHPO Putative G3BP-like protein 102 2e-21
sp|P53741|BRE5_YEAST UBP3-associated protein BRE5 50 1e-05
sp|Q10100|NTF2_SCHPO Nuclear transport factor 2 (NTF-2) 50 1e-05
sp|Q6BWC0|NTF2_DEBHA Nuclear transport factor 2 (NTF-2) 49 2e-05
sp|Q9C7F5|NTF2_ARATH Nuclear transport factor 2 (NTF-2) 48 4e-05
sp|Q9P926|NTF2_CANAL Nuclear transport factor 2 (NTF-2) 48 4e-05
>sp|Q13283|G3BP_HUMAN Ras-GTPase-activating protein binding protein 1 (GAP SH3-domain
binding protein 1) (G3BP-1) (DNA helicase VIII)
(HDH-VIII)
Length = 466
Score = 124 bits (311), Expect = 4e-28
Identities = 99/365 (27%), Positives = 165/365 (45%), Gaps = 53/365 (14%)
Frame = +2
Query: 38 MDRELITTVSQEFVLQFYTIMNKHPGSLHRFYKEESTLIRDEVHAQG--------QNEIH 193
M++ V +EFV Q+YT++N+ P LHRFY + S+ + + + G Q EIH
Sbjct: 3 MEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKEIH 62
Query: 194 KYYMNLELSNCKAVVLSLDAVKSHGKSILIQVTGEIANNGCDLRRFMQSFLLVEQD--LG 367
+ M+ +NC + +DA + +++QV G ++NN LRRFMQ+F+L +
Sbjct: 63 RKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSVAN 122
Query: 368 NFYVLNDIFRYQDQTF-----KVEDVEEAPVVEHESKNE------DIHGEINSWNELSRN 514
FYV NDIFRYQD+ F + ++ E V E E + + D G +S +
Sbjct: 123 KFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQAVVSND 182
Query: 515 CELNNEQIPQSPQLIEHEEGSEEFINYKIMNEEKDISSEIPVEIDSQQ-------DIGQK 673
E + E+ P+ E +E ++ +I E+ + E D+Q+ DI Q
Sbjct: 183 MEEHLEEPVAEPEPDPEPEPEQEPVS-EIQEEKPEPVLEETAPEDAQKSSSPAPADIAQT 241
Query: 674 MEEMNIKEKSWAA--------------------IINPMSSRPSXXXXXXXXXXXXXXXXX 793
++E +++ SWA+ ++ +S+P
Sbjct: 242 VQE-DLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRD 300
Query: 794 XXXINSNGDNMEKRKPR-----FNNGNVKTPSTLNYPDEHQLFVGNLPQNMTEDELKDFF 958
+ +R PR G+++ + +PD HQLF+GNLP + + ELKDFF
Sbjct: 301 QRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSHQLFIGNLPHEVDKSELKDFF 360
Query: 959 SEKYG 973
+ YG
Sbjct: 361 -QSYG 364
>sp|Q9UN86|G3B2_HUMAN Ras-GTPase-activating protein binding protein 2 (GAP SH3-domain
binding protein 2) (G3BP-2)
Length = 482
Score = 119 bits (297), Expect = 2e-26
Identities = 95/356 (26%), Positives = 157/356 (44%), Gaps = 49/356 (13%)
Frame = +2
Query: 38 MDRELITTVSQEFVLQFYTIMNKHPGSLHRFYKEESTLIRDEVHAQG--------QNEIH 193
M++ V +EFV Q+YT++NK P LHRFY S+ + V A G QN+IH
Sbjct: 3 MEKPSPLLVGREFVRQYYTLLNKAPEYLHRFYGRNSSYVHGGVDASGKPQEAVYGQNDIH 62
Query: 194 KYYMNLELSNCKAVVLSLDAVKSHGKSILIQVTGEIANNGCDLRRFMQSFLLVEQDL--G 367
++L S C + +DA + +++QV G ++N+G R+FMQ+F+L +
Sbjct: 63 HKVLSLNFSECHTKIRHVDAHATLSDGVVVQVMGLLSNSGQPERKFMQTFVLAPEGSVPN 122
Query: 368 NFYVLNDIFRYQDQTF---------KVEDVEEAPVVEHESKNEDIHGEINSW----NELS 508
FYV ND+FRY+D+ F + ED E E + E + NS + ++
Sbjct: 123 KFYVHNDMFRYEDEVFGDSEPELDEESEDEVEEEQEERQPSPEPVQENANSGYYEAHPVT 182
Query: 509 RNCELNNEQIPQSPQLIEHEEGSEEFINYKIMNEEKDISSEIPVEIDSQQDIGQKMEEMN 688
E E+ P+ E E + ++ EEK++ E++ + E ++
Sbjct: 183 NGIEEPLEESSHEPEPEPESETKTEELKPQV--EEKNLE-----ELEEKSTTPPPAEPVS 235
Query: 689 IKEK-----SWAAIIN---PMSS--RPSXXXXXXXXXXXXXXXXXXXXINSNGDNMEKRK 838
+ ++ SWA++ + P S S + S + +++
Sbjct: 236 LPQEPPKAFSWASVTSKNLPPSGTVSSSGIPSHVKAPVSQPRVEAKPEVQSQPPRVREQR 295
Query: 839 PRFNNG----------------NVKTPSTLNYPDEHQLFVGNLPQNMTEDELKDFF 958
PR G + + YPD HQLFVGNLP ++ E+ELK+FF
Sbjct: 296 preRPGFPPRGPRPGRGDMEQNDSDNRRIIRYPDSHQLFVGNLPHDIDENELKEFF 351
>sp|P97379|G3B2_MOUSE Ras-GTPase-activating protein binding protein 2 (GAP SH3-domain
binding protein 2) (G3BP-2)
Length = 482
Score = 116 bits (290), Expect = 1e-25
Identities = 95/368 (25%), Positives = 155/368 (42%), Gaps = 61/368 (16%)
Frame = +2
Query: 38 MDRELITTVSQEFVLQFYTIMNKHPGSLHRFYKEESTLIRDEVHAQG--------QNEIH 193
M++ V +EFV Q+YT++NK P LHRFY S+ + V A G QN+IH
Sbjct: 3 MEKPSPLLVGREFVRQYYTLLNKAPEYLHRFYGRNSSYVHGGVDASGKPQEAVYGQNDIH 62
Query: 194 KYYMNLELSNCKAVVLSLDAVKSHGKSILIQVTGEIANNGCDLRRFMQSFLLVEQDL--G 367
++L S C + +DA + +++QV G ++N+G R+FMQ+F+L +
Sbjct: 63 HKVLSLNFSECHTKIRHVDAHATLSDGVVVQVMGLLSNSGQPERKFMQTFVLAPEGSVPN 122
Query: 368 NFYVLNDIFRYQDQTFKVEDVEEAPVVEHESKNEDIHGEINSWNELSRNCELNNEQIPQS 547
FYV ND+FRY+D+ F + P ++ ES++E E E S
Sbjct: 123 KFYVHNDMFRYEDEVFG----DSEPELDEESEDE---------------VEEEQEDRQPS 163
Query: 548 PQLIEHEEGSEEFINYKIMNEEKDISSEIPVEIDSQQDIGQKMEEM--NIKEK------- 700
P+ ++ S + + + N ++ E E + + + K EE+ ++EK
Sbjct: 164 PEPVQENANSAYYDAHPVTNGIEEPLEESSHEPEPEPESETKTEELKPQVEEKHLEELEE 223
Query: 701 ---------------------SWAAIIN---PMSS--RPSXXXXXXXXXXXXXXXXXXXX 802
SWA++ + P S S
Sbjct: 224 KSATPPPAEPASLPQEPPKAFSWASVTSKNLPPSGTVSSSGIPPHVKAPVSQPRVDAKPE 283
Query: 803 INSNGDNMEKRKPRFNNG----------------NVKTPSTLNYPDEHQLFVGNLPQNMT 934
+ S + +++PR G + + YPD HQLFVGNLP ++
Sbjct: 284 VQSQPPRVREQRpreRPGFPPRGPRPGRGDMEQNDSDNRRIIRYPDSHQLFVGNLPHDID 343
Query: 935 EDELKDFF 958
E+ELK+FF
Sbjct: 344 ENELKEFF 351
>sp|P97855|G3BP_MOUSE Ras-GTPase-activating protein binding protein 1 (GAP SH3-domain
binding protein 1) (G3BP-1) (DNA helicase VIII)
(HDH-VIII)
Length = 465
Score = 112 bits (281), Expect = 1e-24
Identities = 95/361 (26%), Positives = 159/361 (44%), Gaps = 54/361 (14%)
Frame = +2
Query: 38 MDRELITTVSQEFVLQFYTIMNKHPGSLHRFYKEESTLIRDEVHAQG--------QNEIH 193
M++ V +EFV Q+YT++N+ P LHRFY + S+ + + G Q EIH
Sbjct: 3 MEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYAHGGLDSNGKPADAVYGQKEIH 62
Query: 194 KYYMNLELSNCKAVVLSLDAVKSHGKSILIQVTGEIANNGCDLRRFMQSFLLVEQD--LG 367
+ M+ +NC + +DA + +++QV G ++NN LRRFMQ+F+L +
Sbjct: 63 RKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSVAN 122
Query: 368 NFYVLNDIFRYQDQTF----------KVEDVEEAPVVEHESKNEDIHGEINSWNE--LSR 511
FYV NDIFRYQD+ F E+VEE E + E + + ++ + +S
Sbjct: 123 KFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPE--ERQQTPEVVPDDSGTFYDQTVSN 180
Query: 512 NCELNNEQIPQSPQLIEHEEGSEEFINYKIMNEEKDISSEIPVEIDSQQ-------DIGQ 670
+ E + E+ P+ E E ++ I ++ + + E D Q+ D+
Sbjct: 181 DLEEHLEEPVVEPEPEPEPEPEPEPVS-DIQEDKPEAALEEAAPDDVQKSTSPAPADVAP 239
Query: 671 KMEEMNIKEKSWAAIIN---------PMSSRPSXXXXXXXXXXXXXXXXXXXXINSNGDN 823
E+ ++ SWA++ + P++ P
Sbjct: 240 AQED--LRTFSWASVTSKNLPPSGAVPVTGTPPHVVKVPASQPRPESKPDSQIPPQRPQR 297
Query: 824 MEK-RKPRFN---------------NGNVKTPSTLNYPDEHQLFVGNLPQNMTEDELKDF 955
++ R+ R N G+V+ + +PD HQLF+GNLP + + ELKDF
Sbjct: 298 DQRVREQRINIPPQRGPRPIREAGEPGDVEPRRMVRHPDSHQLFIGNLPHEVDKSELKDF 357
Query: 956 F 958
F
Sbjct: 358 F 358
>sp|O94260|G3BP_SCHPO Putative G3BP-like protein
Length = 434
Score = 102 bits (254), Expect = 2e-21
Identities = 60/137 (43%), Positives = 87/137 (63%), Gaps = 6/137 (4%)
Frame = +2
Query: 74 FVLQFYTIMNKHPGSLHRFYKEESTLIR-DEVHA----QGQNEIHKYYMNLELSNCKAVV 238
FV ++YT +NK P LH FY ++STLI DE + GQ EIH ++L+ NCK ++
Sbjct: 22 FVQEYYTYLNKEPNRLHCFYTKKSTLIHGDEGESISLCHGQQEIHNKILDLDFQNCKVLI 81
Query: 239 LSLDAVKSHGKSILIQVTGEIANNGCDLRRFMQSFLLVEQDLGNFYVLNDIFRYQDQTFK 418
++D++ S I+IQV GE++N G R+F Q+F L EQ G ++VLNDIFR+ +
Sbjct: 82 SNVDSLASSNGGIVIQVLGEMSNKGKLSRKFAQTFFLAEQPNG-YFVLNDIFRFLRE--D 138
Query: 419 VEDVEEAP-VVEHESKN 466
VE+ EE+P VE E K+
Sbjct: 139 VEEEEESPDAVEKEKKD 155
>sp|P53741|BRE5_YEAST UBP3-associated protein BRE5
Length = 515
Score = 50.1 bits (118), Expect = 1e-05
Identities = 40/152 (26%), Positives = 71/152 (46%), Gaps = 21/152 (13%)
Frame = +2
Query: 74 FVLQFYTIMNKHPGSL-----------HRFYKEESTLIRDEV----HAQGQNEIHKYYMN 208
F+ +Y M P L H Y+ +ST +D+V G+ I+K++
Sbjct: 12 FLQNYYERMRTDPSKLAYFYASTAELTHTNYQSKSTNEKDDVLPTVKVTGRENINKFFSR 71
Query: 209 LELSNCKAVVLSLDAVKSH-----GKSILIQVTGEIANNGCDLRRFMQSFLLVEQDLGN- 370
+ + +++ L LD + KSILI TGE+ G + +F Q+F+L+ G+
Sbjct: 72 ND-AKVRSLKLKLDTIDFQYTGHLHKSILIMATGEMFWTGTPVYKFCQTFILLPSSNGST 130
Query: 371 FYVLNDIFRYQDQTFKVEDVEEAPVVEHESKN 466
F + NDI R+ +FK + +A + + +N
Sbjct: 131 FDITNDIIRFISNSFKPYVLTDASLSQSNEEN 162
>sp|Q10100|NTF2_SCHPO Nuclear transport factor 2 (NTF-2)
Length = 123
Score = 50.1 bits (118), Expect = 1e-05
Identities = 34/114 (29%), Positives = 59/114 (51%), Gaps = 2/114 (1%)
Frame = +2
Query: 62 VSQEFVLQFYTIMNKHPGSLHRFYKEESTLIRDEVHAQGQNEIHKYYMNLELSNCKAVVL 241
++ +F +Y + L Y+EES L + QG I + ++L + +
Sbjct: 7 LATQFTQFYYQTFDSDRSQLSSLYREESMLSFEGAQLQGTKAIVEKLVSLPFQRVQHRIS 66
Query: 242 SLDAVKSHGK-SILIQVTGEIANNGCDL-RRFMQSFLLVEQDLGNFYVLNDIFR 397
+LDA + S+++ VTGE+ + + +R+ Q F LV + GN+YVLND+FR
Sbjct: 67 TLDAQPTGTTGSVIVMVTGELLLDEEQMAQRYSQVFHLVNNN-GNYYVLNDLFR 119
>sp|Q6BWC0|NTF2_DEBHA Nuclear transport factor 2 (NTF-2)
Length = 124
Score = 49.3 bits (116), Expect = 2e-05
Identities = 37/115 (32%), Positives = 58/115 (50%), Gaps = 2/115 (1%)
Frame = +2
Query: 59 TVSQEFVLQFYTIMNKHPGSLHRFYKEESTLIRDEVHAQGQNEIHKYYMNLELSNCKAVV 238
TV+ EF +Y + L Y+E+S L + QG +I + ++L +
Sbjct: 7 TVASEFCNFYYQQFDSDRTQLGNLYREQSMLTFETSQLQGAKDIVEKLVSLPFQKVAHRI 66
Query: 239 LSLDAVK-SHGKSILIQVTGE-IANNGCDLRRFMQSFLLVEQDLGNFYVLNDIFR 397
+LDA S IL+ VTGE I ++ + +R+ Q F L+ D ++YV NDIFR
Sbjct: 67 STLDAQPGSPNGDILVMVTGELIIDDEQNAQRYSQVFHLI-PDGNSYYVFNDIFR 120
>sp|Q9C7F5|NTF2_ARATH Nuclear transport factor 2 (NTF-2)
Length = 126
Score = 48.1 bits (113), Expect = 4e-05
Identities = 36/114 (31%), Positives = 55/114 (48%), Gaps = 2/114 (1%)
Frame = +2
Query: 62 VSQEFVLQFYTIMNKHPGSLHRFYKEESTLIRDEVHAQGQNEIHKYYMNLELSNCKAVVL 241
VS+ FV +Y+ + + L Y+E S L + QG I +L CK +
Sbjct: 9 VSKAFVEHYYSTFDTNRVGLAGLYQEASMLTFEGQKIQGVQSIVAKLTSLPFQQCKHHIS 68
Query: 242 SLDAVKSHGKS-ILIQVTGEIANNGCD-LRRFMQSFLLVEQDLGNFYVLNDIFR 397
++D S S +L+ V+G + G + +F Q F L+ G+FYV NDIFR
Sbjct: 69 TVDCQPSGPASGMLVFVSGNLQLAGEEHALKFSQMFHLMPTPQGSFYVFNDIFR 122
>sp|Q9P926|NTF2_CANAL Nuclear transport factor 2 (NTF-2)
Length = 124
Score = 48.1 bits (113), Expect = 4e-05
Identities = 35/114 (30%), Positives = 55/114 (48%), Gaps = 2/114 (1%)
Frame = +2
Query: 62 VSQEFVLQFYTIMNKHPGSLHRFYKEESTLIRDEVHAQGQNEIHKYYMNLELSNCKAVVL 241
V+ EF +Y + L Y+ ES L + QG +I + +L +
Sbjct: 8 VATEFCNFYYNQFDSDRSQLGNLYRNESMLTFETSQLQGARDIVEKLASLPFQKVAHRIS 67
Query: 242 SLDAVKSHGKS-ILIQVTGEIA-NNGCDLRRFMQSFLLVEQDLGNFYVLNDIFR 397
+LDA + IL+ VTGE+ + + +R+ Q F L+ D G++YV NDIFR
Sbjct: 68 TLDAQPASANGDILVMVTGELLIDEEQNAQRYSQVFHLIP-DNGSYYVFNDIFR 120
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 96,086,874
Number of Sequences: 369166
Number of extensions: 1770651
Number of successful extensions: 6503
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6470
length of database: 68,354,980
effective HSP length: 111
effective length of database: 47,849,395
effective search space used: 10191921135
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)