Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_005_F12
(716 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P59780|AP3S2_HUMAN Adapter-related protein complex 3 sig... 266 4e-71
sp|Q92572|AP3S1_HUMAN Adapter-related protein complex 3 sig... 259 5e-69
sp|Q09905|YAJ5_SCHPO Adaptin complex small chain homolog C3... 168 1e-41
sp|P47064|AP22_YEAST Probable adaptin complex small chain h... 130 4e-30
sp|P61967|AP1S1_MOUSE Adapter-related protein complex 1 sig... 114 3e-25
sp|P56377|AP1S2_HUMAN Adapter-related protein complex 1 sig... 110 3e-24
sp|Q9DB50|AP1S2_MOUSE Adapter-related protein complex 1 sig... 109 6e-24
sp|P62743|AP2S1_MOUSE Clathrin coat assembly protein AP17 (... 108 1e-23
sp|Q9Y587|AP4S1_HUMAN Adapter-related protein complex 4 sig... 105 2e-22
sp|Q96PC3|AP1S3_HUMAN Adapter-related protein complex 1 sig... 104 3e-22
>sp|P59780|AP3S2_HUMAN Adapter-related protein complex 3 sigma 2 subunit (Sigma-adaptin
3b) (AP-3 complex sigma-3B subunit) (Sigma-3B-adaptin)
sp|Q8BSZ2|AP3S2_MOUSE Adapter-related protein complex 3 sigma 2 subunit (Sigma-adaptin
3b) (AP-3 complex sigma-3B subunit) (Sigma-3B-adaptin)
sp|Q5RDP9|AP3S2_PONPY Adapter-related protein complex 3 sigma 2 subunit (Sigma-adaptin
3b) (AP-3 complex sigma-3B subunit) (Sigma-3B-adaptin)
Length = 193
Score = 266 bits (680), Expect = 4e-71
Identities = 127/182 (69%), Positives = 158/182 (86%)
Frame = +3
Query: 57 MIQAILIFNNHGKPRMIKFYEYYTEEMQQKIVQETFQLVSKRSDDVCNFLESGSLINGKD 236
MIQAIL+FNNHGKPR+++FY+ + EE+QQ+IV+ETF LV KR D++CNFLE GSLI G D
Sbjct: 1 MIQAILVFNNHGKPRLVRFYQRFPEEIQQQIVRETFHLVLKRDDNICNFLEGGSLIGGSD 60
Query: 237 NKLVYRHYATLYFVFCVDSSESELGILDLIQVFVESLDKAFENVCELDLIFNIDKVHHIL 416
KL+YRHYATLYFVFCVDSSESELGILDLIQVFVE+LDK FENVCELDLIF++DKVH+IL
Sbjct: 61 YKLIYRHYATLYFVFCVDSSESELGILDLIQVFVETLDKCFENVCELDLIFHMDKVHYIL 120
Query: 417 KEVVLGGLVLDTHMTEILSRFEEQNKLEREENGLSIKSGSAVSAVKSINIPQRIRDIKLT 596
+EVV+GG+VL+T+M EI+++ E QN+LE+ E GLS AVSAVK+IN+P+ R+I +
Sbjct: 121 QEVVMGGMVLETNMNEIVAQIEAQNRLEKSEGGLSAAPARAVSAVKNINLPEIPRNINIG 180
Query: 597 DL 602
DL
Sbjct: 181 DL 182
>sp|Q92572|AP3S1_HUMAN Adapter-related protein complex 3 sigma 1 subunit (Sigma-adaptin
3a) (AP-3 complex sigma-3A subunit) (Sigma-3A-adaptin)
(Clathrin-associated/assembly/adapter protein, small 3)
sp|Q9DCR2|AP3S1_MOUSE Adapter-related protein complex 3 sigma 1 subunit (Sigma-adaptin
3a) (AP-3 complex sigma-3A subunit) (Sigma-3A-adaptin)
Length = 193
Score = 259 bits (662), Expect = 5e-69
Identities = 124/182 (68%), Positives = 157/182 (86%)
Frame = +3
Query: 57 MIQAILIFNNHGKPRMIKFYEYYTEEMQQKIVQETFQLVSKRSDDVCNFLESGSLINGKD 236
MI+AILIFNNHGKPR+ KFY+ Y+E+ QQ+I++ETF LVSKR ++VCNFLE G LI G D
Sbjct: 1 MIKAILIFNNHGKPRLSKFYQPYSEDTQQQIIRETFHLVSKRDENVCNFLEGGLLIGGSD 60
Query: 237 NKLVYRHYATLYFVFCVDSSESELGILDLIQVFVESLDKAFENVCELDLIFNIDKVHHIL 416
NKL+YRHYATLYFVFCVDSSESELGILDLIQVFVE+LDK FENVCELDLIF++DKVH+IL
Sbjct: 61 NKLIYRHYATLYFVFCVDSSESELGILDLIQVFVETLDKCFENVCELDLIFHVDKVHNIL 120
Query: 417 KEVVLGGLVLDTHMTEILSRFEEQNKLEREENGLSIKSGSAVSAVKSINIPQRIRDIKLT 596
E+V+GG+VL+T+M EI+++ + QNKLE+ E GL+ AVSAVK++N+P+ R+I +
Sbjct: 121 AEMVMGGMVLETNMNEIVTQIDAQNKLEKSEAGLAGAPARAVSAVKNMNLPEIPRNINIG 180
Query: 597 DL 602
D+
Sbjct: 181 DI 182
>sp|Q09905|YAJ5_SCHPO Adaptin complex small chain homolog C30D11.05
Length = 165
Score = 168 bits (426), Expect = 1e-41
Identities = 86/154 (55%), Positives = 109/154 (70%)
Frame = +3
Query: 57 MIQAILIFNNHGKPRMIKFYEYYTEEMQQKIVQETFQLVSKRSDDVCNFLESGSLINGKD 236
MI A+ IFNN GKPR+ KFY E +QQK++ + + VS R CNFLES +LI GK
Sbjct: 1 MIYAVFIFNNKGKPRLTKFYTPIDESIQQKLIGDIYAAVSTRPPTACNFLES-NLIAGK- 58
Query: 237 NKLVYRHYATLYFVFCVDSSESELGILDLIQVFVESLDKAFENVCELDLIFNIDKVHHIL 416
N+++YR YATLYFVF VD ESELGILDLIQVFVE+LD+ F NVCELDL+F ++H IL
Sbjct: 59 NRIIYRQYATLYFVFVVDEGESELGILDLIQVFVEALDRCFNNVCELDLVFKFQEIHAIL 118
Query: 417 KEVVLGGLVLDTHMTEILSRFEEQNKLEREENGL 518
EVV GGLVL+T++ EI+ + Q + N +
Sbjct: 119 AEVVSGGLVLETNLNEIVLAAQNQMPKTKRSNAM 152
>sp|P47064|AP22_YEAST Probable adaptin complex small chain homolog
Length = 194
Score = 130 bits (327), Expect = 4e-30
Identities = 67/158 (42%), Positives = 103/158 (65%), Gaps = 12/158 (7%)
Frame = +3
Query: 57 MIQAILIFNNHGKPRMIKFYEYYTEEMQQKIVQETFQLVSKRSDDV-CNFL--------- 206
MI A+LIFN +PR++KFY Q+ ++++ ++L+S+R+ D +FL
Sbjct: 1 MIHAVLIFNKKCQPRLVKFYTPVDLPKQKLLLEQVYELISQRNSDFQSSFLVTPPSLLLS 60
Query: 207 --ESGSLINGKDNKLVYRHYATLYFVFCVDSSESELGILDLIQVFVESLDKAFENVCELD 380
+ +N +D +++Y++YATLYF F VD ESEL ILDLIQ FVESLD+ F V ELD
Sbjct: 61 NENNNDEVNNEDIQIIYKNYATLYFTFIVDDQESELAILDLIQTFVESLDRCFTEVNELD 120
Query: 381 LIFNIDKVHHILKEVVLGGLVLDTHMTEILSRFEEQNK 494
LIFN + +L+E+V GG+V++T++ I++ +E NK
Sbjct: 121 LIFNWQTLESVLEEIVQGGMVIETNVNRIVASVDELNK 158
>sp|P61967|AP1S1_MOUSE Adapter-related protein complex 1 sigma 1A subunit (Sigma-adaptin
1A) (Adaptor protein complex AP-1 sigma-1A subunit)
(Golgi adaptor HA1/AP1 adaptin sigma-1A subunit)
(Clathrin assembly protein complex 1 sigma-1A small
chain) (Clathrin coat assembly protein AP19) (HA1 19 kDa
subunit) (Sigma 1a subunit of AP-1 clathrin)
sp|P61966|AP1S1_HUMAN Adapter-related protein complex 1 sigma 1A subunit (Sigma-adaptin
1A) (Adaptor protein complex AP-1 sigma-1A subunit)
(Golgi adaptor HA1/AP1 adaptin sigma-1A subunit)
(Clathrin assembly protein complex 1 sigma-1A small
chain) (Clathrin coat assembly protein AP19) (HA1 19 kDa
subunit) (Sigma 1a subunit of AP-1 clathrin)
Length = 158
Score = 114 bits (284), Expect = 3e-25
Identities = 59/151 (39%), Positives = 97/151 (64%)
Frame = +3
Query: 57 MIQAILIFNNHGKPRMIKFYEYYTEEMQQKIVQETFQLVSKRSDDVCNFLESGSLINGKD 236
M++ +L+F+ GK R+ K+Y +++ ++K+V+E Q+V R +C+FLE +D
Sbjct: 1 MMRFMLLFSRQGKLRLQKWYLATSDKERKKMVRELMQVVLARKPKMCSFLE------WRD 54
Query: 237 NKLVYRHYATLYFVFCVDSSESELGILDLIQVFVESLDKAFENVCELDLIFNIDKVHHIL 416
K+VY+ YA+LYF ++ ++EL L+LI +VE LDK F +VCELD+IFN +K + IL
Sbjct: 55 LKVVYKRYASLYFCCAIEGQDNELITLELIHRYVELLDKYFGSVCELDIIFNFEKAYFIL 114
Query: 417 KEVVLGGLVLDTHMTEILSRFEEQNKLEREE 509
E ++GG V DT +L E+ + L+ E+
Sbjct: 115 DEFLMGGDVQDTSKKSVLKAIEQADLLQEED 145
>sp|P56377|AP1S2_HUMAN Adapter-related protein complex 1 sigma 1B subunit (Sigma-adaptin
1B) (Adaptor protein complex AP-1 sigma-1B subunit)
(Golgi adaptor HA1/AP1 adaptin sigma-1B subunit)
(Clathrin assembly protein complex 1 sigma-1B small
chain) (Sigma 1B subunit of AP-1 clathrin)
Length = 157
Score = 110 bits (276), Expect = 3e-24
Identities = 58/149 (38%), Positives = 93/149 (62%)
Frame = +3
Query: 60 IQAILIFNNHGKPRMIKFYEYYTEEMQQKIVQETFQLVSKRSDDVCNFLESGSLINGKDN 239
+Q +L+F+ GK R+ K+Y +++ ++KI +E Q V R +C+FLE +D
Sbjct: 1 MQFMLLFSRQGKLRLQKWYVPLSDKEKKKITRELVQTVLARKPKMCSFLE------WRDL 54
Query: 240 KLVYRHYATLYFVFCVDSSESELGILDLIQVFVESLDKAFENVCELDLIFNIDKVHHILK 419
K+VY+ YA+LYF ++ ++EL L++I +VE LDK F +VCELD+IFN +K + IL
Sbjct: 55 KIVYKRYASLYFCCAIEDQDNELITLEIIHRYVELLDKYFGSVCELDIIFNFEKAYFILD 114
Query: 420 EVVLGGLVLDTHMTEILSRFEEQNKLERE 506
E +LGG V +T +L E+ + L+ E
Sbjct: 115 EFLLGGEVQETSKKNVLKAIEQADLLQEE 143
>sp|Q9DB50|AP1S2_MOUSE Adapter-related protein complex 1 sigma 1B subunit (Sigma-adaptin
1B) (Adaptor protein complex AP-1 sigma-1B subunit)
(Golgi adaptor HA1/AP1 adaptin sigma-1B subunit)
(Clathrin assembly protein complex 1 sigma-1B small
chain) (Sigma 1B subunit of AP-1 clathrin)
Length = 160
Score = 109 bits (273), Expect = 6e-24
Identities = 57/149 (38%), Positives = 93/149 (62%)
Frame = +3
Query: 60 IQAILIFNNHGKPRMIKFYEYYTEEMQQKIVQETFQLVSKRSDDVCNFLESGSLINGKDN 239
+Q +L+F+ GK R+ K+Y +++ ++KI +E Q V R +C+FLE +D
Sbjct: 1 MQFMLLFSRQGKLRLQKWYVPLSDKEKKKITRELVQTVLARKPKMCSFLE------WRDL 54
Query: 240 KLVYRHYATLYFVFCVDSSESELGILDLIQVFVESLDKAFENVCELDLIFNIDKVHHILK 419
K+VY+ YA+LYF ++ ++EL L++I +VE LDK F +VCELD+IFN +K + IL
Sbjct: 55 KIVYKRYASLYFCCAIEDQDNELITLEIIHRYVELLDKYFGSVCELDIIFNFEKAYFILD 114
Query: 420 EVVLGGLVLDTHMTEILSRFEEQNKLERE 506
E +LGG V +T +L E+ + L+ +
Sbjct: 115 EFLLGGEVQETSKKNVLKAIEQADLLQED 143
>sp|P62743|AP2S1_MOUSE Clathrin coat assembly protein AP17 (Clathrin coat-associated
protein AP17) (Plasma membrane adaptor AP-2 17 kDa
protein) (Clathrin assembly protein 2 small chain)
sp|P62744|AP2S1_RAT Clathrin coat assembly protein AP17 (Clathrin coat-associated
protein AP17) (Plasma membrane adaptor AP-2 17 kDa
protein) (Clathrin assembly protein 2 small chain)
sp|Q5R940|AP2S1_PONPY Clathrin coat assembly protein AP17 (Clathrin coat-associated
protein AP17) (Plasma membrane adaptor AP-2 17 kDa
protein) (Clathrin assembly protein 2 small chain)
sp|P53680|AP2S1_HUMAN Clathrin coat assembly protein AP17 (Clathrin coat-associated
protein AP17) (Plasma membrane adaptor AP-2 17 kDa
protein) (HA2 17 kDa subunit) (Clathrin assembly protein
2 small chain)
Length = 142
Score = 108 bits (270), Expect = 1e-23
Identities = 57/148 (38%), Positives = 90/148 (60%)
Frame = +3
Query: 57 MIQAILIFNNHGKPRMIKFYEYYTEEMQQKIVQETFQLVSKRSDDVCNFLESGSLINGKD 236
MI+ ILI N GK R+ K+Y + ++ +QK+++E +V+ R NF+E ++
Sbjct: 1 MIRFILIQNRAGKTRLAKWYMQFDDDEKQKLIEEVHAVVTVRDAKHTNFVEF------RN 54
Query: 237 NKLVYRHYATLYFVFCVDSSESELGILDLIQVFVESLDKAFENVCELDLIFNIDKVHHIL 416
K++YR YA LYF CVD +++ L L+ I FVE L++ F NVCELDL+FN KV+ ++
Sbjct: 55 FKIIYRRYAGLYFCICVDVNDNNLAYLEAIHNFVEVLNEYFHNVCELDLVFNFYKVYTVV 114
Query: 417 KEVVLGGLVLDTHMTEILSRFEEQNKLE 500
E+ L G + +T T++L + LE
Sbjct: 115 DEMFLAGEIRETSQTKVLKQLLMLQSLE 142
>sp|Q9Y587|AP4S1_HUMAN Adapter-related protein complex 4 sigma 1 subunit (Sigma subunit of
AP-4) (AP-4 adapter complex sigma subunit)
Length = 144
Score = 105 bits (261), Expect = 2e-22
Identities = 55/139 (39%), Positives = 85/139 (61%)
Frame = +3
Query: 57 MIQAILIFNNHGKPRMIKFYEYYTEEMQQKIVQETFQLVSKRSDDVCNFLESGSLINGKD 236
MI+ L+ N G+ R+ K+YE+ + + E + RS++ C+F+E KD
Sbjct: 1 MIKFFLMVNKQGQTRLSKYYEHVDINKRTLLETEVIKSCLSRSNEQCSFIEY------KD 54
Query: 237 NKLVYRHYATLYFVFCVDSSESELGILDLIQVFVESLDKAFENVCELDLIFNIDKVHHIL 416
KL+YR YA L+ V V+ +E+E+ I + I FVE LD+ F V ELD++FN+DKVH IL
Sbjct: 55 FKLIYRQYAALFIVVGVNDTENEMAIYEFIHNFVEVLDEYFSRVSELDIMFNLDKVHIIL 114
Query: 417 KEVVLGGLVLDTHMTEILS 473
E+VL G +++T+ IL+
Sbjct: 115 DEMVLNGCIVETNRARILA 133
>sp|Q96PC3|AP1S3_HUMAN Adapter-related protein complex 1 sigma 1C subunit (Sigma-adaptin
1C) (Adaptor protein complex AP-1 sigma-1C subunit)
(Golgi adaptor HA1/AP1 adaptin sigma-1C subunit)
(Clathrin assembly protein complex 1 sigma-1C small
chain) (Sigma 1C subunit of AP-1 clathrin)
Length = 154
Score = 104 bits (259), Expect = 3e-22
Identities = 53/148 (35%), Positives = 90/148 (60%)
Frame = +3
Query: 57 MIQAILIFNNHGKPRMIKFYEYYTEEMQQKIVQETFQLVSKRSDDVCNFLESGSLINGKD 236
MI IL+F+ GK R+ K+Y ++ ++KI +E Q++ R + S ++ K+
Sbjct: 1 MIHFILLFSRQGKLRLQKWYITLPDKERKKITREIVQIILSRGH------RTSSFVDWKE 54
Query: 237 NKLVYRHYATLYFVFCVDSSESELGILDLIQVFVESLDKAFENVCELDLIFNIDKVHHIL 416
KLVY+ YA+LYF +++ ++EL L+++ +VE LDK F NVCELD+IFN +K + IL
Sbjct: 55 LKLVYKRYASLYFCCAIENQDNELLTLEIVHRYVELLDKYFGNVCELDIIFNFEKAYFIL 114
Query: 417 KEVVLGGLVLDTHMTEILSRFEEQNKLE 500
E ++GG + +T + E+ + L+
Sbjct: 115 DEFIIGGEIQETSKKIAVKAIEDSDMLQ 142
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 64,103,965
Number of Sequences: 369166
Number of extensions: 1123481
Number of successful extensions: 3521
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3408
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3503
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6365071885
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)