Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_005_F03
(679 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 122 9e-28
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 120 3e-27
sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinas... 117 2e-26
sp|Q13153|PAK1_HUMAN Serine/threonine-protein kinase PAK 1 ... 114 3e-25
sp|P35465|PAK1_RAT Serine/threonine-protein kinase PAK 1 (p... 112 9e-25
sp|O88643|PAK1_MOUSE Serine/threonine-protein kinase PAK 1 ... 111 2e-24
sp|Q01389|BCK1_YEAST Serine/threonine-protein kinase BCK1/S... 110 3e-24
sp|P28829|BYR2_SCHPO Protein kinase byr2 (Protein kinase st... 110 3e-24
sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinas... 110 3e-24
sp|Q64303|PAK2_RAT Serine/threonine-protein kinase PAK 2 (p... 110 4e-24
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 122 bits (306), Expect = 9e-28
Identities = 73/188 (38%), Positives = 105/188 (55%), Gaps = 1/188 (0%)
Frame = +1
Query: 13 IKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNN 192
I L+++ GS+ S L K GA E + ++ Q+L L++LH+ I+HRD+KG IL+DN
Sbjct: 148 ILLEFVPGGSISSLLEKFGAFPESVVRTYTNQLLLGLEYLHNHAIMHRDIKGANILVDNQ 207
Query: 193 LDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVM 372
IKL F ++K + ++ + A+S GT W APEVI G+ SADIWS G +
Sbjct: 208 GCIKLADFGASKQVAELATIS----GAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTV 263
Query: 373 VELITGKAPLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLKRPTVK 549
+E++TGKAP AI+ +GT K P S F+ CL EP RPT
Sbjct: 264 IEMVTGKAPWSQQYKEIAAIFHIGTTKS-HPPIPDNISSDANDFLLKCLQQEPNLRPTAS 322
Query: 550 DLMKHEFL 573
+L+KH F+
Sbjct: 323 ELLKHPFV 330
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 120 bits (302), Expect = 3e-27
Identities = 74/188 (39%), Positives = 106/188 (56%), Gaps = 1/188 (0%)
Frame = +1
Query: 13 IKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNN 192
I L+++ GS+ S L K G E + +++Q+L L++LH+ I+HRD+KG IL+DN
Sbjct: 149 ILLEFVPGGSISSLLEKFGPFPESVVRTYTRQLLLGLEYLHNHAIMHRDIKGANILVDNK 208
Query: 193 LDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVM 372
IKL F ++K + ++ T A+S GT W APEVI G+ SADIWS G +
Sbjct: 209 GCIKLADFGASKQVAELATMT----GAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTV 264
Query: 373 VELITGKAPLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLKRPTVK 549
+E++TGKAP AI+ +GT K SDA K F+ CL P RPT
Sbjct: 265 IEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPDTLSSDA-KDFLLKCLQEVPNLRPTAS 323
Query: 550 DLMKHEFL 573
+L+KH F+
Sbjct: 324 ELLKHPFV 331
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
NPK1-related protein kinase 3)
Length = 651
Score = 117 bits (294), Expect = 2e-26
Identities = 70/188 (37%), Positives = 106/188 (56%), Gaps = 1/188 (0%)
Frame = +1
Query: 13 IKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNN 192
I ++++ GS+ S L K G+ EP II ++KQ+L L++LH+ I+HRD+KG IL+DN
Sbjct: 148 ILMEFVPGGSISSLLEKFGSFPEPVIIMYTKQLLLGLEYLHNNGIMHRDIKGANILVDNK 207
Query: 193 LDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVM 372
I+L F ++K + ++ + A+S GT W APEVI G+ SADIWS G +
Sbjct: 208 GCIRLADFGASKKVVELATV----NGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTV 263
Query: 373 VELITGKAPLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLKRPTVK 549
+E+ TGK P F A+ +G + K P S K F+ CL EP R +
Sbjct: 264 IEMATGKPPWSEQYQQFAAVLHIG-RTKAHPPIPEDLSPEAKDFLMKCLHKEPSLRLSAT 322
Query: 550 DLMKHEFL 573
+L++H F+
Sbjct: 323 ELLQHPFV 330
>sp|Q13153|PAK1_HUMAN Serine/threonine-protein kinase PAK 1 (p21-activated kinase 1)
(PAK-1) (P65-PAK) (Alpha-PAK)
Length = 545
Score = 114 bits (284), Expect = 3e-25
Identities = 64/192 (33%), Positives = 103/192 (53%), Gaps = 1/192 (0%)
Frame = +1
Query: 10 YIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDN 189
++ ++Y+A GSL + + E I ++ L+AL+FLHS ++HRD+K ILL
Sbjct: 341 WVVMEYLAGGSLTDVVTET-CMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGM 399
Query: 190 NLDIKLCGF-MSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGA 366
+ +KL F A++ P+ S + + GT W APEV+ + YG DIWS G
Sbjct: 400 DGSVKLTDFGFCAQITPEQSKRS-------TMVGTPYWMAPEVVTRKAYGPKVDIWSLGI 452
Query: 367 VMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLKRPTV 546
+ +E+I G+ P +A+Y + T E P K S + F++ CLD + KR +
Sbjct: 453 MAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLDMDVEKRGSA 512
Query: 547 KDLMKHEFLNSA 582
K+L++H+FL A
Sbjct: 513 KELLQHQFLKIA 524
>sp|P35465|PAK1_RAT Serine/threonine-protein kinase PAK 1 (p21-activated kinase 1)
(PAK-1) (P68-PAK) (Alpha-PAK) (Protein kinase MUK2)
Length = 544
Score = 112 bits (280), Expect = 9e-25
Identities = 63/192 (32%), Positives = 103/192 (53%), Gaps = 1/192 (0%)
Frame = +1
Query: 10 YIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDN 189
++ ++Y+A GSL + + E I ++ L+AL+FLHS ++HRD+K ILL
Sbjct: 340 WVVMEYLAGGSLTDVVTET-CMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGM 398
Query: 190 NLDIKLCGF-MSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGA 366
+ +KL F A++ P+ S + + GT W APEV+ + YG DIWS G
Sbjct: 399 DGSVKLTDFGFCAQITPEQSKRS-------TMVGTPYWMAPEVVTRKAYGPKVDIWSLGI 451
Query: 367 VMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLKRPTV 546
+ +E+I G+ P +A+Y + T E P K S + F++ CL+ + KR +
Sbjct: 452 MAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSA 511
Query: 547 KDLMKHEFLNSA 582
K+L++H+FL A
Sbjct: 512 KELLQHQFLKIA 523
>sp|O88643|PAK1_MOUSE Serine/threonine-protein kinase PAK 1 (p21-activated kinase 1)
(PAK-1) (P65-PAK) (Alpha-PAK) (CDC42/RAC effector kinase
PAK-A)
Length = 545
Score = 111 bits (278), Expect = 2e-24
Identities = 63/192 (32%), Positives = 102/192 (53%), Gaps = 1/192 (0%)
Frame = +1
Query: 10 YIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDN 189
++ ++Y+A GSL + + E I ++ L+AL+FLHS ++HRD+K ILL
Sbjct: 341 WVVMEYLAGGSLTDVVTET-CMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGM 399
Query: 190 NLDIKLCGF-MSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGA 366
+ +KL F A++ P+ S + + GT W APEV+ + YG DIWS G
Sbjct: 400 DGSVKLTDFGFCAQITPEQSKRS-------TMVGTPYWMAPEVVTRKAYGPKVDIWSLGI 452
Query: 367 VMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLKRPTV 546
+ +E+I G+ P +A+Y + T E P K S + F+ CL+ + KR +
Sbjct: 453 MAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLQCCLEMDVEKRGSA 512
Query: 547 KDLMKHEFLNSA 582
K+L++H+FL A
Sbjct: 513 KELLQHQFLKIA 524
>sp|Q01389|BCK1_YEAST Serine/threonine-protein kinase BCK1/SLK1/SSP31
Length = 1478
Score = 110 bits (276), Expect = 3e-24
Identities = 65/189 (34%), Positives = 101/189 (53%), Gaps = 5/189 (2%)
Frame = +1
Query: 19 LDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNNLD 198
L+Y+A GS+ S + G EP I + Q+L+ L +LHS+ ILHRD+K +LLD +
Sbjct: 1257 LEYVAGGSVGSLIRMYGRFDEPLIKHLTTQVLKGLAYLHSKGILHRDMKADNLLLDQDGI 1316
Query: 199 IKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVIN-GEGYGSSADIWSFGAVMV 375
K+ F ++ D S + + GT W APE+++ +GY + DIWS G +++
Sbjct: 1317 CKISDFGISRKSKDIYSNS-----DMTMRGTVFWMAPEMVDTKQGYSAKVDIWSLGCIVL 1371
Query: 376 ELITGKAPLFFLASFQAIYRLGTQKKI----EFAYPLKCSDALKSFVDPCLDPEPLKRPT 543
E+ GK P L A++++G K E PL S ++F+D C + P KRPT
Sbjct: 1372 EMFAGKRPWSNLEVVAAMFKIGKSKSAPPIPEDTLPL-ISQIGRNFLDACFEINPEKRPT 1430
Query: 544 VKDLMKHEF 570
+L+ H F
Sbjct: 1431 ANELLSHPF 1439
>sp|P28829|BYR2_SCHPO Protein kinase byr2 (Protein kinase ste8) (MAPK kinase kinase)
(MAPKKK)
Length = 659
Score = 110 bits (275), Expect = 3e-24
Identities = 64/188 (34%), Positives = 99/188 (52%)
Frame = +1
Query: 13 IKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNN 192
I L+Y+ GS+ L G+ +E + F KQ L+ L++LHS+ I+HRD+KG IL+DN
Sbjct: 474 IFLEYVPGGSVAGLLTMYGSFEETLVKNFIKQTLKGLEYLHSRGIVHRDIKGANILVDNK 533
Query: 193 LDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVM 372
IK+ F +K S+ T S G++ W APEV+ + DIWS G ++
Sbjct: 534 GKIKISDFGISKKLELNSTSTKTGGARPSFQGSSFWMAPEVVKQTMHTEKTDIWSLGCLV 593
Query: 373 VELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLKRPTVKD 552
+E++T K P QAI+R+G EF P S + F++ + RPT +
Sbjct: 594 IEMLTSKHPYPNCDQMQAIFRIGENILPEF--PSNISSSAIDFLEKTFAIDCNLRPTASE 651
Query: 553 LMKHEFLN 576
L+ H F++
Sbjct: 652 LLSHPFVS 659
>sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 618
Score = 110 bits (275), Expect = 3e-24
Identities = 67/192 (34%), Positives = 104/192 (54%)
Frame = +1
Query: 1 KASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKIL 180
K I ++YM GS++ L GA E ++++QILE + +LHS ILHRD+KG IL
Sbjct: 430 KTLSIFMEYMPGGSIKDQLKAYGALTENGTRKYTRQILEGVHYLHSNMILHRDIKGANIL 489
Query: 181 LDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSF 360
D+ ++KL F ++K + +S GT W +PEVI+G+GYG ADIWS
Sbjct: 490 RDSTGNVKLGDFGASKRL---QTICLSGTGMKSVTGTPYWMSPEVISGQGYGRKADIWSV 546
Query: 361 GAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLKRP 540
+VE++T K P + AI+++ TQ P SD + F+ + E RP
Sbjct: 547 ACTVVEMLTEKPPWAEFEAMAAIFKIATQ-PTNPKLPPHVSDYTRDFLKR-IFVEAKLRP 604
Query: 541 TVKDLMKHEFLN 576
+ +L++H F++
Sbjct: 605 SADELLRHMFVH 616
>sp|Q64303|PAK2_RAT Serine/threonine-protein kinase PAK 2 (p21-activated kinase 2)
(PAK-2) (Gamma-PAK) (p62-PAK)
Length = 524
Score = 110 bits (274), Expect = 4e-24
Identities = 60/192 (31%), Positives = 101/192 (52%), Gaps = 1/192 (0%)
Frame = +1
Query: 10 YIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDN 189
++ ++Y+A GSL + + E I ++ L+AL+FLH+ ++HRD+K +LL
Sbjct: 320 FVVMEYLAGGSLTDVVTET-CMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGM 378
Query: 190 NLDIKLCGF-MSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGA 366
+KL F A++ P+ S + + GT W APEV+ + YG DIWS G
Sbjct: 379 EGSVKLTDFGFCAQITPEQSKRS-------TMVGTPYWMAPEVVTRKAYGPKVDIWSLGI 431
Query: 367 VMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLKRPTV 546
+ +E++ G+ P +A+Y + T E P K S + F++ CL+ + KR +
Sbjct: 432 MAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGSA 491
Query: 547 KDLMKHEFLNSA 582
K+L++H FL A
Sbjct: 492 KELLQHPFLKLA 503
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 82,142,335
Number of Sequences: 369166
Number of extensions: 1681622
Number of successful extensions: 7539
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5715
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6029
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 5733423530
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)