Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_005_F02
(954 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q16222|UAP1_HUMAN UDP-N-acetylhexosamine pyrophosphoryla... 182 2e-45
sp|Q91YN5|UAP1_MOUSE UDP-N-acetylhexosamine pyrophosphoryla... 179 8e-45
sp|P43123|UAP1_YEAST UDP-N-acetylglucosamine pyrophosphorylase 153 6e-37
sp|Q18493|UAP1_CAEEL Probable UDP-N-acetylglucosamine pyrop... 152 2e-36
sp|O64765|UAP1_ARATH Probable UDP-N-acetylglucosamine pyrop... 149 1e-35
sp|O74933|UAP1_CANAL UDP-N-acetylglucosamine pyrophosphorylase 141 2e-33
sp|O94617|UAP1_SCHPO Probable UDP-N-acetylglucosamine pyrop... 125 2e-28
sp|P09662|C10AA_BACTI Pesticidal crystal protein cry10Aa (I... 31 4.5
sp|P47267|SYM_MYCGE Methionyl-tRNA synthetase (Methionine--... 31 5.9
sp|Q04511|UFO1_YEAST Ubiquitin ligase complex F-box protein... 30 10.0
>sp|Q16222|UAP1_HUMAN UDP-N-acetylhexosamine pyrophosphorylase (Antigen X) (AGX)
(Sperm-associated antigen 2) [Includes:
UDP-N-acetylgalactosamine pyrophosphorylase (AGX-1);
UDP-N-acetylglucosamine pyrophosphorylase (AGX-2)]
Length = 522
Score = 182 bits (461), Expect = 2e-45
Identities = 100/193 (51%), Positives = 129/193 (66%), Gaps = 1/193 (0%)
Frame = +3
Query: 3 KSRGIKYALVYGVDNILVKIVDPVFLGFCVEKGAECAAKVVEKVHPKEAVGIVGKVNGCY 182
+ RGI VY VDNILVK+ DP F+GFC++KGA+C AKVVEK +P E VG+V +V+G Y
Sbjct: 240 EQRGIWSIHVYCVDNILVKVADPRFIGFCIQKGADCGAKVVEKTNPTEPVGVVCRVDGVY 299
Query: 183 QVIEYSEISLETATMRDIKNDKLLYRCGSICTHLFSVDFLSRFCQNNVNNLLLQHIAIKK 362
QV+EYSEISL TA R + +LL+ G+I H F+V FL R N L H+A KK
Sbjct: 300 QVVEYSEISLATAQKRS-SDGRLLFNAGNIANHFFTVPFL-RDVVNVYEPQLQHHVAQKK 357
Query: 363 VPFINIQTGILEIPEKENAIKMEKFVFDVFPLAKTFAIWEVERNREFSALKNAPGSQ-SD 539
+P+++ Q G L P+K N IKMEKFVFD+F AK F ++EV R EFS LKNA D
Sbjct: 358 IPYVDTQ-GQLIKPDKPNGIKMEKFVFDIFQFAKKFVVYEVLREDEFSPLKNADSQNGKD 416
Query: 540 CPETAKNDLLAYH 578
P TA++ L++ H
Sbjct: 417 NPTTARHALMSLH 429
>sp|Q91YN5|UAP1_MOUSE UDP-N-acetylhexosamine pyrophosphorylase [Includes:
UDP-N-acetylgalactosamine pyrophosphorylase ;
UDP-N-acetylglucosamine pyrophosphorylase ]
Length = 522
Score = 179 bits (455), Expect = 8e-45
Identities = 98/193 (50%), Positives = 128/193 (66%), Gaps = 1/193 (0%)
Frame = +3
Query: 3 KSRGIKYALVYGVDNILVKIVDPVFLGFCVEKGAECAAKVVEKVHPKEAVGIVGKVNGCY 182
+ RGI VY VDNILVK+ DP F+GFC++KGA+C AKVVEK +P E VG+V +V+G Y
Sbjct: 240 EQRGICSIHVYCVDNILVKVADPRFIGFCIQKGADCGAKVVEKTNPTEPVGVVCRVDGVY 299
Query: 183 QVIEYSEISLETATMRDIKNDKLLYRCGSICTHLFSVDFLSRFCQNNVNNLLLQHIAIKK 362
QV+EYSEISL TA R + +LL+ G+I H F+V FL + N L H+A KK
Sbjct: 300 QVVEYSEISLATAQRRS-SDGRLLFNAGNIANHFFTVPFL-KDVVNVYEPQLQHHVAQKK 357
Query: 363 VPFINIQTGILEIPEKENAIKMEKFVFDVFPLAKTFAIWEVERNREFSALKNAPGSQ-SD 539
+P+++ Q G P+K N IKMEKFVFD+F AK F ++EV R EFS LKNA D
Sbjct: 358 IPYVDSQ-GYFIKPDKPNGIKMEKFVFDIFQFAKKFVVYEVLREDEFSPLKNADSQNGKD 416
Query: 540 CPETAKNDLLAYH 578
P TA++ L++ H
Sbjct: 417 NPTTARHALMSLH 429
>sp|P43123|UAP1_YEAST UDP-N-acetylglucosamine pyrophosphorylase
Length = 477
Score = 153 bits (387), Expect = 6e-37
Identities = 106/253 (41%), Positives = 143/253 (56%), Gaps = 7/253 (2%)
Frame = +3
Query: 9 RGIKYALVYGVDNILVKIVDPVFLGFCVEKGAECAAKVVEKVHPKEAVGIVGKVNGCYQV 188
RGIK+ +Y VDN+L KI DPVF+GF ++ G E A K V K E+VG++ N V
Sbjct: 241 RGIKHVYMYCVDNVLSKIADPVFIGFAIKHGFELATKAVRKRDAHESVGLIATKNEKPCV 300
Query: 189 IEYSEISLETATMRDIKNDKLLYRCGSICTHLFSVDFLSR----FCQNNVNNLLLQHIAI 356
IEYSEIS E A +D K+ L R G+I H + VD L R +C+N + HIA
Sbjct: 301 IEYSEISNELAEAKD-KDGLLKLRAGNIVNHYYLVDLLKRDLDQWCEN-----MPYHIAK 354
Query: 357 KKVPFINIQTGILEIPEKENAIKMEKFVFDVF---PLAKTFAIWEVERNREFSALKNAPG 527
KK+P + TG P + N IK+E+F+FDVF PL K F EV+R +EFS LKN PG
Sbjct: 355 KKIPAYDSVTGKYTKPTEPNGIKLEQFIFDVFDTVPLNK-FGCLEVDRCKEFSPLKNGPG 413
Query: 528 SQSDCPETAKNDLLAYHRQXXXXXXXXXXXXKEKVKKTIIELSPLLTYSGEDMGNISVLI 707
S++D PET++ LAY + K+ V ++E+S L+Y+GE N+S
Sbjct: 414 SKNDNPETSR---LAYLKLGTSWLEDAGAIVKDGV---LVEVSSKLSYAGE---NLSQFK 464
Query: 708 KNSGDESIVQLEK 746
D S + LEK
Sbjct: 465 GKVFDRSGIVLEK 477
>sp|Q18493|UAP1_CAEEL Probable UDP-N-acetylglucosamine pyrophosphorylase
Length = 484
Score = 152 bits (383), Expect = 2e-36
Identities = 92/228 (40%), Positives = 131/228 (57%)
Frame = +3
Query: 3 KSRGIKYALVYGVDNILVKIVDPVFLGFCVEKGAECAAKVVEKVHPKEAVGIVGKVNGCY 182
+++GIKY VY VDNIL K+ DP F+GF + A+ A K V K E VG V G
Sbjct: 242 RAKGIKYFHVYCVDNILCKVADPHFIGFAISNEADVATKCVPK-QKGELVGSVCLDRGLP 300
Query: 183 QVIEYSEISLETATMRDIKNDKLLYRCGSICTHLFSVDFLSRFCQNNVNNLLLQHIAIKK 362
+V+EYSE+ E A + + K L+ GSI H F++DF+ R C + ++ L H A KK
Sbjct: 301 RVVEYSELGAELAEQKT-PDGKYLFGAGSIANHFFTMDFMDRVC--SPSSRLPYHRAHKK 357
Query: 363 VPFINIQTGILEIPEKENAIKMEKFVFDVFPLAKTFAIWEVERNREFSALKNAPGSQSDC 542
+ ++N Q I++ PEK N IK+E+F+FDVF L+K F IWEV RN EFS LKNA +DC
Sbjct: 358 ISYVNEQGTIVK-PEKPNGIKLEQFIFDVFELSKRFFIWEVARNEEFSPLKNAQSVGTDC 416
Query: 543 PETAKNDLLAYHRQXXXXXXXXXXXXKEKVKKTIIELSPLLTYSGEDM 686
T + DL ++ K + I L +++Y+GE++
Sbjct: 417 LSTCQRDLSNVNK-----LWLERVQAKVTATEKPIYLKTIVSYNGENL 459
>sp|O64765|UAP1_ARATH Probable UDP-N-acetylglucosamine pyrophosphorylase
Length = 502
Score = 149 bits (376), Expect = 1e-35
Identities = 87/230 (37%), Positives = 134/230 (58%), Gaps = 3/230 (1%)
Frame = +3
Query: 6 SRGIKYALVYGVDNILVKIVDPVFLGFCVEKGAECAAKVVEKVHPKEAVGIV---GKVNG 176
SRGIKY YGVDN+LV++ DP FLG+ ++K A AAKVV K +P+E VG+ GK G
Sbjct: 268 SRGIKYVDCYGVDNVLVRVADPTFLGYFIDKSAASAAKVVRKAYPQEKVGVFVRRGK-GG 326
Query: 177 CYQVIEYSEISLETATMRDIKNDKLLYRCGSICTHLFSVDFLSRFCQNNVNNLLLQHIAI 356
V+EY+E+ A+ + + +L Y ++C H+F++DFL++ N + + H+A
Sbjct: 327 PLTVVEYTELDQSMASATNQQTGRLQYCWSNVCLHMFTLDFLNQVA-NGLEKDSVYHLAE 385
Query: 357 KKVPFINIQTGILEIPEKENAIKMEKFVFDVFPLAKTFAIWEVERNREFSALKNAPGSQS 536
KK+P IN +K+E+F+FD FP A + A++EV R EF+ +KNA GS
Sbjct: 386 KKIPSINGDI---------VGLKLEQFIFDCFPYAPSTALFEVLREEEFAPVKNANGSNY 436
Query: 537 DCPETAKNDLLAYHRQXXXXXXXXXXXXKEKVKKTIIELSPLLTYSGEDM 686
D PE+A+ +L H + + T +E+SPL +Y+GE++
Sbjct: 437 DTPESARLLVLRLHTR-WVIAAGGFLTHSVPLYATGVEVSPLCSYAGENL 485
>sp|O74933|UAP1_CANAL UDP-N-acetylglucosamine pyrophosphorylase
Length = 486
Score = 141 bits (356), Expect = 2e-33
Identities = 83/195 (42%), Positives = 120/195 (61%), Gaps = 7/195 (3%)
Frame = +3
Query: 6 SRGIKYALVYGVDNILVKIVDPVFLGFCVEKGAECAAKVVEKVHPKEAVGIV--GKVNGC 179
S+GIK+ +Y VDN LVK+ DP+F+GF + K + A KVV K E+VG++ + N
Sbjct: 245 SKGIKHIHMYCVDNCLVKVADPIFIGFAIAKKFDLATKVVRKRDANESVGLIVLDQDNQK 304
Query: 180 YQVIEYSEISLETATMRDIK-NDKLLYRCGSICTHLFSVDFLSRFCQNNVNN--LLLQHI 350
VIEYSEIS E A +D + + KL R +I H +SV+FL++ +++ L HI
Sbjct: 305 PCVIEYSEISQELANKKDPQDSSKLFLRAANIVNHYYSVEFLNKMIPKWISSQKYLPFHI 364
Query: 351 AIKKVPFINIQTGILEIPEKENAIKMEKFVFDVFPLAK--TFAIWEVERNREFSALKNAP 524
A KK+P +N++ G P + N IK+E+F+FDVFP + F EV+R EFS LKNA
Sbjct: 365 AKKKIPSLNLENGEFYKPTEPNGIKLEQFIFDVFPSVELNKFGCLEVDRLDEFSPLKNAD 424
Query: 525 GSQSDCPETAKNDLL 569
G+++D P T +N L
Sbjct: 425 GAKNDTPTTCRNHYL 439
>sp|O94617|UAP1_SCHPO Probable UDP-N-acetylglucosamine pyrophosphorylase
Length = 475
Score = 125 bits (314), Expect = 2e-28
Identities = 77/192 (40%), Positives = 106/192 (55%), Gaps = 4/192 (2%)
Frame = +3
Query: 9 RGIKYALVYGVDNILVKIVDPVFLGFCVEKGAECAAKVVEKVHPKEAVGIVGKVNGCYQV 188
RGI + Y VDN+LV VDPVF+G K E A K VEK+ P E VG++ + V
Sbjct: 240 RGILHITAYSVDNVLVLPVDPVFIGMATTKKLEVATKTVEKIDPAEKVGLLVSSHNHPCV 299
Query: 189 IEYSEISLET--ATMRDIKNDKLLYRCGSICTHLFSVDFLSRFCQNNVNNLLLQHIAIKK 362
+EYSEIS E AT + LL R +I H FS DFL + + ++ L H+A KK
Sbjct: 300 VEYSEISDEACKATENVDGHKHLLLRAANIAYHYFSFDFLQKASLH--SSTLPIHLACKK 357
Query: 363 VPFINIQTGILEIPEKENAIKMEKFVFDVFP--LAKTFAIWEVERNREFSALKNAPGSQS 536
+PF ++ + P N K+E F+FD+FP + F ++V R FS LKN+ S +
Sbjct: 358 IPFYDVTSHHYTTPLNPNGYKLESFIFDLFPSVSVENFGCFQVPRRTSFSPLKNSSKSPN 417
Query: 537 DCPETAKNDLLA 572
D ET ND+L+
Sbjct: 418 DNHETCVNDILS 429
>sp|P09662|C10AA_BACTI Pesticidal crystal protein cry10Aa (Insecticidal delta-endotoxin
CryXA(a)) (Crystaline entomocidal protoxin) (78 kDa
crystal protein)
Length = 675
Score = 31.2 bits (69), Expect = 4.5
Identities = 15/36 (41%), Positives = 22/36 (61%)
Frame = +3
Query: 345 HIAIKKVPFINIQTGILEIPEKENAIKMEKFVFDVF 452
+I I K+ FI I +L+ EK+N K +K V D+F
Sbjct: 638 NIYIDKIEFIPITQSVLDYTEKQNIEKTQKIVNDLF 673
>sp|P47267|SYM_MYCGE Methionyl-tRNA synthetase (Methionine--tRNA ligase) (MetRS)
Length = 512
Score = 30.8 bits (68), Expect = 5.9
Identities = 24/104 (23%), Positives = 49/104 (47%), Gaps = 7/104 (6%)
Frame = +3
Query: 225 MRDIKNDKLLYRCGSICTHLFSVDFLSRFCQNNVNNLLLQHIAIKKVPFINIQTGILEIP 404
+++I N L G+ + F + + ++ + ++ A++K+ I I I++
Sbjct: 345 LKEIYNADLANSFGNHVSRTFGM--IKKYLNGKLEYQIITDNALQKI-MILIDESIVQFD 401
Query: 405 EKENAIK-------MEKFVFDVFPLAKTFAIWEVERNREFSALK 515
N+ + + K VF++ L F WE+ +N+EFS LK
Sbjct: 402 HYFNSYEFYRAINLLLKIVFELSKLIDDFKPWELFKNQEFSLLK 445
>sp|Q04511|UFO1_YEAST Ubiquitin ligase complex F-box protein UFO1
Length = 668
Score = 30.0 bits (66), Expect = 10.0
Identities = 25/110 (22%), Positives = 46/110 (41%)
Frame = +3
Query: 108 CAAKVVEKVHPKEAVGIVGKVNGCYQVIEYSEISLETATMRDIKNDKLLYRCGSICTHLF 287
CA ++ EK +E +GCY + ++ +L +RD+ + L +C
Sbjct: 329 CAVEIDEKTSQREQNWQFAGDDGCYISLLTTQNTLYIINIRDLSSSGLKVQC-------- 380
Query: 288 SVDFLSRFCQNNVNNLLLQHIAIKKVPFINIQTGILEIPEKENAIKMEKF 437
+ F + + V NL++ + +N TG L + +K EKF
Sbjct: 381 KISFDEQVYVSQVTNLIVVVALPNVLQILNAMTGEL----IKTVLKTEKF 426
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 100,698,779
Number of Sequences: 369166
Number of extensions: 1927060
Number of successful extensions: 4925
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4753
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4906
length of database: 68,354,980
effective HSP length: 111
effective length of database: 47,849,395
effective search space used: 9856975370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)