Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_005_D20
(886 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O00519|FAAH_HUMAN Fatty-acid amide hydrolase (Oleamide h... 220 5e-57
sp|O08914|FAAH_MOUSE Fatty-acid amide hydrolase (Oleamide h... 219 6e-57
sp|P97612|FAAH_RAT Fatty-acid amide hydrolase (Oleamide hyd... 219 8e-57
sp|Q9TUI8|FAAH_PIG Fatty-acid amide hydrolase (Oleamide hyd... 217 3e-56
sp|Q90578|VDHAP_CHICK Vitamin D3 hydroxylase-associated pro... 198 2e-50
sp|P08158|AMDS_EMENI Acetamidase 107 4e-23
sp|Q12559|AMDS_ASPOR Acetamidase 100 7e-21
sp|P22580|AMDY_YEAST Probable amidase 94 7e-19
sp|Q9LCX3|GATA_THET8 Glutamyl-tRNA(Gln) amidotransferase su... 93 9e-19
sp|O27955|GATA_ARCFU Glutamyl-tRNA(Gln) amidotransferase su... 89 1e-17
>sp|O00519|FAAH_HUMAN Fatty-acid amide hydrolase (Oleamide hydrolase) (Anandamide
amidohydrolase)
Length = 579
Score = 220 bits (560), Expect = 5e-57
Identities = 121/295 (41%), Positives = 172/295 (58%)
Frame = +1
Query: 1 EVMKALESGVYKPIDILQAYQAKALIMDKEYNFITDLNPNVDVDVLGGDTSKRGLLKGIP 180
++++ L S P +L Y KA ++K N +T + + + ++GLL G+P
Sbjct: 81 QLVQKLHSRELAPEAVLFTYVGKAWEVNKGTNCVTSYLADCETQL--SQAPRQGLLYGVP 138
Query: 181 VSVKECFLIKGCDSTFGMLDRIYCPAERDDMMVRTLRLQGMVPFVSTNLCQSGLSIESSN 360
VS+KECF KG DST G+ PAE D ++V L+LQG VPFV TN+ QS S + SN
Sbjct: 139 VSLKECFTYKGQDSTLGLSLNEGVPAECDSVVVHVLKLQGAVPFVHTNVPQSMFSYDCSN 198
Query: 361 PIFGETLNPHNKTRTXXXXXXXXXXXXXXXXXLVGLGSDIGGSIRIPSSYCGVYGLKPTS 540
P+FG+T+NP +++ +GLG+DIGGSIR PSS+CG+ GLKPT
Sbjct: 199 PLFGQTVNPWKSSKSPGGSSGGEGALIGSGGSPLGLGTDIGGSIRFPSSFCGICGLKPTG 258
Query: 541 QRFSSRHGKYLFPDQNIINVSVGPMARNIEDLVAVFKAMACGDLYHLDPYCPPLCFNDKI 720
R S K Q + +SVGPMAR++E L +A+ C D++ LDP PPL F +++
Sbjct: 259 NRLSKSGLKGCVYGQEAVRLSVGPMARDVESLALCLRALLCEDMFRLDPTVPPLPFREEV 318
Query: 721 FDKKRPLRIGYYIHLGENQVTPVPAVVRAVQIAKKSLESAGHVLIEFNFPNIDYA 885
+ +PLR+GYY +N P PA+ RAV K+SLE+AGH L+ F NI +A
Sbjct: 319 YTSSQPLRVGYY--ETDNYTMPSPAMRRAVLETKQSLEAAGHTLVPFLPSNIPHA 371
>sp|O08914|FAAH_MOUSE Fatty-acid amide hydrolase (Oleamide hydrolase) (Anandamide
amidohydrolase)
Length = 579
Score = 219 bits (559), Expect = 6e-57
Identities = 122/295 (41%), Positives = 173/295 (58%)
Frame = +1
Query: 1 EVMKALESGVYKPIDILQAYQAKALIMDKEYNFITDLNPNVDVDVLGGDTSKRGLLKGIP 180
++++ L+SG P +L Y KA ++K N +T + + + ++GLL G+P
Sbjct: 81 QLVQKLQSGELSPEAVLFTYLGKAWEVNKGTNCVTSYLTDCETQL--SQAPRQGLLYGVP 138
Query: 181 VSVKECFLIKGCDSTFGMLDRIYCPAERDDMMVRTLRLQGMVPFVSTNLCQSGLSIESSN 360
VS+KECF KG ST G+ +E D ++V+ L+LQG VPFV TN+ QS LS + SN
Sbjct: 139 VSLKECFSYKGHASTLGLSLNEGVTSESDCVVVQVLKLQGAVPFVHTNVPQSMLSYDCSN 198
Query: 361 PIFGETLNPHNKTRTXXXXXXXXXXXXXXXXXLVGLGSDIGGSIRIPSSYCGVYGLKPTS 540
P+FG+T+NP +++ +GLG+DIGGSIR PS++CG+ GLKPT
Sbjct: 199 PLFGQTMNPWKPSKSPGGSSGGEGALIGSGGSPLGLGTDIGGSIRFPSAFCGICGLKPTG 258
Query: 541 QRFSSRHGKYLFPDQNIINVSVGPMARNIEDLVAVFKAMACGDLYHLDPYCPPLCFNDKI 720
R S K Q + +SVGPMAR+++ L KA+ C DL+ LD PPL F ++I
Sbjct: 259 NRLSKSGLKSCVYGQTAVQLSVGPMARDVDSLALCMKALLCEDLFRLDSTIPPLPFREEI 318
Query: 721 FDKKRPLRIGYYIHLGENQVTPVPAVVRAVQIAKKSLESAGHVLIEFNFPNIDYA 885
+ RPLR+GYY +N P PA+ RAV K+SLE+AGH L+ F NI YA
Sbjct: 319 YRSSRPLRVGYY--ETDNYTMPTPAMRRAVMETKQSLEAAGHTLVPFLPNNIPYA 371
>sp|P97612|FAAH_RAT Fatty-acid amide hydrolase (Oleamide hydrolase) (Anandamide
amidohydrolase)
Length = 579
Score = 219 bits (558), Expect = 8e-57
Identities = 121/295 (41%), Positives = 173/295 (58%)
Frame = +1
Query: 1 EVMKALESGVYKPIDILQAYQAKALIMDKEYNFITDLNPNVDVDVLGGDTSKRGLLKGIP 180
++++ L+SG P + Y KA ++K N +T + + + ++GLL G+P
Sbjct: 81 QLVQKLQSGELSPEAVFFTYLGKAWEVNKGTNCVTSYLTDCETQL--SQAPRQGLLYGVP 138
Query: 181 VSVKECFLIKGCDSTFGMLDRIYCPAERDDMMVRTLRLQGMVPFVSTNLCQSGLSIESSN 360
VS+KECF KG DST G+ P+E D ++V+ L+LQG VPFV TN+ QS LS + SN
Sbjct: 139 VSLKECFSYKGHDSTLGLSLNEGMPSESDCVVVQVLKLQGAVPFVHTNVPQSMLSFDCSN 198
Query: 361 PIFGETLNPHNKTRTXXXXXXXXXXXXXXXXXLVGLGSDIGGSIRIPSSYCGVYGLKPTS 540
P+FG+T+NP +++ +GLG+DIGGSIR PS++CG+ GLKPT
Sbjct: 199 PLFGQTMNPWKSSKSPGGSSGGEGALIGSGGSPLGLGTDIGGSIRFPSAFCGICGLKPTG 258
Query: 541 QRFSSRHGKYLFPDQNIINVSVGPMARNIEDLVAVFKAMACGDLYHLDPYCPPLCFNDKI 720
R S K Q + +S+GPMAR++E L KA+ C L+ LDP PPL F +++
Sbjct: 259 NRLSKSGLKGCVYGQTAVQLSLGPMARDVESLALCLKALLCEHLFTLDPTVPPLPFREEV 318
Query: 721 FDKKRPLRIGYYIHLGENQVTPVPAVVRAVQIAKKSLESAGHVLIEFNFPNIDYA 885
+ RPLR+GYY +N P PA+ RA+ K+ LE+AGH LI F NI YA
Sbjct: 319 YRSSRPLRVGYY--ETDNYTMPSPAMRRALIETKQRLEAAGHTLIPFLPNNIPYA 371
>sp|Q9TUI8|FAAH_PIG Fatty-acid amide hydrolase (Oleamide hydrolase) (Anandamide
amidohydrolase)
Length = 579
Score = 217 bits (553), Expect = 3e-56
Identities = 120/295 (40%), Positives = 174/295 (58%)
Frame = +1
Query: 1 EVMKALESGVYKPIDILQAYQAKALIMDKEYNFITDLNPNVDVDVLGGDTSKRGLLKGIP 180
++++ + SG P +L +Y KA +++ N +T + + + +GLL G+P
Sbjct: 81 QLVQKVRSGELSPEAVLFSYLQKAWEVNRGTNCVTTYLADCEAQLC--QAPGQGLLYGVP 138
Query: 181 VSVKECFLIKGCDSTFGMLDRIYCPAERDDMMVRTLRLQGMVPFVSTNLCQSGLSIESSN 360
VS+KECF KG DST G+ PAE D ++V+ L+LQG VPFV TN+ QS S + SN
Sbjct: 139 VSLKECFSCKGHDSTLGLSRNQGTPAECDCVVVQVLKLQGAVPFVHTNVPQSMFSYDCSN 198
Query: 361 PIFGETLNPHNKTRTXXXXXXXXXXXXXXXXXLVGLGSDIGGSIRIPSSYCGVYGLKPTS 540
P+FG+T NP +++ +GLG+DIGGSIR PS++CG+ G+KPT
Sbjct: 199 PLFGQTTNPWMSSKSPGGSSGGEGALIAAGGSPLGLGTDIGGSIRFPSAFCGICGIKPTG 258
Query: 541 QRFSSRHGKYLFPDQNIINVSVGPMARNIEDLVAVFKAMACGDLYHLDPYCPPLCFNDKI 720
R S K Q + +SVGPMAR++E L +A+ C D++ LDP PPL FN+++
Sbjct: 259 NRISKSGLKGSVYGQVAVQLSVGPMARDVESLALCLRALLCEDMFRLDPTVPPLPFNEEV 318
Query: 721 FDKKRPLRIGYYIHLGENQVTPVPAVVRAVQIAKKSLESAGHVLIEFNFPNIDYA 885
+ RPLR+GYY +N P PA+ RA+ K+SLE+AGH LI F NI +A
Sbjct: 319 YASSRPLRVGYY--ETDNYTMPTPAMRRALLETKRSLEAAGHTLIPFLPANIPHA 371
>sp|Q90578|VDHAP_CHICK Vitamin D3 hydroxylase-associated protein (VDHAP)
Length = 464
Score = 198 bits (503), Expect = 2e-50
Identities = 110/295 (37%), Positives = 169/295 (57%), Gaps = 1/295 (0%)
Frame = +1
Query: 1 EVMKALESGVYKPIDILQAYQAKALIMDKEYNFITDLNPNVDVDVLGGDTSK-RGLLKGI 177
E+ + L+ G P +L +Y KAL +++E N + D + + K +GLL GI
Sbjct: 86 ELAEKLKEGSLSPESVLYSYMGKALEVNREVNCVIDFIHGCEDQLQKVKQQKEKGLLYGI 145
Query: 178 PVSVKECFLIKGCDSTFGMLDRIYCPAERDDMMVRTLRLQGMVPFVSTNLCQSGLSIESS 357
PVS+K+ KG S+ G++ + E D ++V+ L+ QG +PFV TN+ Q+ ++ + S
Sbjct: 146 PVSIKDHIDCKGHVSSAGLVKFLGQVKEEDSVIVQVLKSQGAIPFVKTNIPQTMINYDCS 205
Query: 358 NPIFGETLNPHNKTRTXXXXXXXXXXXXXXXXXLVGLGSDIGGSIRIPSSYCGVYGLKPT 537
N IFG+TLNP N +T L+G+GSD+ GSIR+PSS+CG+ GLKPT
Sbjct: 206 NLIFGQTLNPLNHQKTPGGSSGGEGALIAGGGSLLGIGSDVAGSIRLPSSFCGLCGLKPT 265
Query: 538 SQRFSSRHGKYLFPDQNIINVSVGPMARNIEDLVAVFKAMACGDLYHLDPYCPPLCFNDK 717
R S N + +GP+AR+++ L KA+ C +++ LDP PP+ F+++
Sbjct: 266 GFRISKLGVISPITGMNSVIGMLGPIARDVDSLALCMKALLCEEMFRLDPTVPPIPFDEE 325
Query: 718 IFDKKRPLRIGYYIHLGENQVTPVPAVVRAVQIAKKSLESAGHVLIEFNFPNIDY 882
++ +PLRIGYY G Q P P++ RAVQ +K L+ AGH ++ F P IDY
Sbjct: 326 VYTSSKPLRIGYYEEDGYFQ--PSPSMKRAVQQTRKLLQEAGHTIVPFAPPKIDY 378
>sp|P08158|AMDS_EMENI Acetamidase
Length = 548
Score = 107 bits (267), Expect = 4e-23
Identities = 68/237 (28%), Positives = 116/237 (48%), Gaps = 6/237 (2%)
Frame = +1
Query: 160 GLLKGIPVSVKECFLIKGCDSTFGMLDRIYCPAERDDMMVRTLRLQGMVPFVSTNLCQSG 339
G L G+P+S+K+ +KG +++ G + + E D ++ LR G V +V T++ Q+
Sbjct: 119 GPLHGLPISLKDQLRVKGYETSMGYISWLNKYDEGDSVLTTMLRKAGAVFYVKTSVPQTL 178
Query: 340 LSIESSNPIFGETLNPHNKTRTXXXXXXXXXXXXXXXXXLVGLGSDIGGSIRIPSSYCGV 519
+ E+ N I G T+NP NK + ++G+G+DIGGSIR+P+++ +
Sbjct: 179 MVCETVNNIIGRTVNPRNKNWSCGGSSGGEGAIVGIRGGVIGVGTDIGGSIRVPAAFNFL 238
Query: 520 YGLKPTSQRFSSRHGKYLFPDQNIINVSVGPMARNIEDLVAVFKAMACGDLYHLDPYCPP 699
YGL+P+ R Q ++ VGP+ ++EDL K++ + + D P
Sbjct: 239 YGLRPSHGRLPYAKMANSMEGQETVHSVVGPITHSVEDLRLFTKSVLGQEPWKYDSKVIP 298
Query: 700 LCFNDKIFD------KKRPLRIGYYIHLGENQVTPVPAVVRAVQIAKKSLESAGHVL 852
+ + D K L IGYY + V P P ++R V+ +L AGH +
Sbjct: 299 MPWRQSESDIIASKIKNGGLNIGYYNF--DGNVLPHPPILRGVETTVAALAKAGHTV 353
>sp|Q12559|AMDS_ASPOR Acetamidase
Length = 545
Score = 100 bits (248), Expect = 7e-21
Identities = 75/299 (25%), Positives = 136/299 (45%), Gaps = 15/299 (5%)
Frame = +1
Query: 1 EVMKALESGVYKPIDILQAYQAKALIMDKEYNFITDLNPNVDVDVLGG-----DTSKR-- 159
E++ L +G K +D+ A+ +A + + N D P + + +T K+
Sbjct: 60 ELVAQLANGTLKSVDVTLAFCKRAALAHQLVNCAHDFFPELALAQARELDRYFETHKKPV 119
Query: 160 GLLKGIPVSVKECFLIKGCDSTFGMLDRIYCPAERDDMMVRTLRLQGMVPFVSTNLCQSG 339
G L G+P+S+K+ +KG ++ + + D ++ LR G V V T++ Q+
Sbjct: 120 GPLHGLPISLKDQLRVKGTETCMAYISWLGKRDTSDSILTALLRKAGAVFLVKTSVPQTL 179
Query: 340 LSIESSNPIFGETLNPHNKTRTXXXXXXXXXXXXXXXXXLVGLGSDIGGSIRIPSSYCGV 519
+ E+ N I G T NP N + +G+G+DIGGSIR+P+++ +
Sbjct: 180 MVCETVNNIIGRTSNPRNLNLSCGGSSGGEGAMIAMRGGAIGIGTDIGGSIRVPAAFNSL 239
Query: 520 YGLKPTSQRFSSRHGKYLFPDQNIINVSVGPMARNIEDLVAVFKAMACGDLYHLDPYCPP 699
YG++P+ R Q I+ VGP+A + +D+ +++ + + D P
Sbjct: 240 YGIRPSHDRLPYGGMTNSMEGQETIHSVVGPIAHSAQDVRLFLQSVLKEEPWKYDSKVIP 299
Query: 700 LCFND--------KIFDKKRPLRIGYYIHLGENQVTPVPAVVRAVQIAKKSLESAGHVL 852
L + + KI +K L +Y + V P P + R V+I + +LE GH +
Sbjct: 300 LPWREAEENAAQAKIAEKS--LNFAFYDF--DGVVRPHPPITRGVEIVRSTLEKDGHTV 354
>sp|P22580|AMDY_YEAST Probable amidase
Length = 549
Score = 93.6 bits (231), Expect = 7e-19
Identities = 67/219 (30%), Positives = 108/219 (49%), Gaps = 3/219 (1%)
Frame = +1
Query: 166 LKGIPVSVKECFLIKGCDSTFGMLDRIYCPAERDD--MMVRTLRLQGMVPFVSTNLCQSG 339
L GIP+S+K+ ++G D++ G L R + P +++ ++V LR G + FV T + S
Sbjct: 124 LYGIPISLKDQCNVEGVDTSLGYLCRTFKPKTKNEESLIVSFLRDLGAIIFVKTTVPSSM 183
Query: 340 LSIESSNPIFGETLNPHNKTRTXXXXXXXXXXXXXXXXXLVGLGSDIGGSIRIPSSYCGV 519
++ ++ + FG T N N + + L+GLG+DIGGSIRIPSSY G+
Sbjct: 184 MATDTQSNTFGYTYNSINLSFSSGGSSGGEGSLIGAHGSLLGLGTDIGGSIRIPSSYQGL 243
Query: 520 YGLKPTSQRFSSRHGKYLFPDQNIINVSVGPMARNIEDLVAVFKAMA-CGDLYHLDPYCP 696
+GLKPT R F + I +GP+AR++ DL + + D C
Sbjct: 244 FGLKPTFGRVPYLRVDNSFEGRETIPSVIGPLARDLSDLRYFMSCVINICQPWVQDVKCI 303
Query: 697 PLCFNDKIFDKKRPLRIGYYIHLGENQVTPVPAVVRAVQ 813
P F+ +G I G+ + P P+ +RA++
Sbjct: 304 PYHFDSSTSKLHDNYVVG--IWYGDGVIDPPPSDIRALK 340
>sp|Q9LCX3|GATA_THET8 Glutamyl-tRNA(Gln) amidotransferase subunit A (Glu-ADT subunit A)
Length = 471
Score = 93.2 bits (230), Expect = 9e-19
Identities = 84/298 (28%), Positives = 128/298 (42%), Gaps = 3/298 (1%)
Frame = +1
Query: 1 EVMKALESGVYKPIDILQAYQAKALIMDKEYNFITDLNPNVDVDVLGGDTSKRGL-LKGI 177
E+ + G P+++ QAY + +D LN + + D GL L G+
Sbjct: 5 EIRARVARGEVSPLEVAQAYLKRVQELDPGLGAFLSLNERLLEEAEAVDP---GLPLAGL 61
Query: 178 PVSVKECFLIKGCDSTFG--MLDRIYCPAERDDMMVRTLRLQGMVPFVSTNLCQSGLSIE 351
V+VK+ +G +T G +L+ P E V L+ G + TNL + G+
Sbjct: 62 VVAVKDNIATRGLRTTAGSRLLENFVPPYEAT--AVARLKALGALVLGKTNLDEFGMGSS 119
Query: 352 SSNPIFGETLNPHNKTRTXXXXXXXXXXXXXXXXXLVGLGSDIGGSIRIPSSYCGVYGLK 531
+ + F T NP + R + LGSD GGS+R P+++CGVYGLK
Sbjct: 120 TEHSAFFPTKNPFDPDRVPGGSSGGSAAALAADLAPLALGSDTGGSVRQPAAFCGVYGLK 179
Query: 532 PTSQRFSSRHGKYLFPDQNIINVSVGPMARNIEDLVAVFKAMACGDLYHLDPYCPPLCFN 711
PT R SR G + +GPMAR++ DL + A A D P F
Sbjct: 180 PTYGRV-SRFGLIAYASS---LDQIGPMARSVRDLALLMDAAAGPDPLDATSLDLPPRFQ 235
Query: 712 DKIFDKKRPLRIGYYIHLGENQVTPVPAVVRAVQIAKKSLESAGHVLIEFNFPNIDYA 885
+ + PLR+G + E P V RA++ A K G + E ++P++ A
Sbjct: 236 EALEGPLPPLRLGV---VREALAGNSPGVERALEEALKVFRELGLSVREVSWPSLPQA 290
>sp|O27955|GATA_ARCFU Glutamyl-tRNA(Gln) amidotransferase subunit A (Glu-ADT subunit A)
Length = 457
Score = 89.4 bits (220), Expect = 1e-17
Identities = 76/245 (31%), Positives = 112/245 (45%), Gaps = 2/245 (0%)
Frame = +1
Query: 157 RGLLKGIPVSVKECFLIKGCDSTFG--MLDRIYCPAERDDMMVRTLRLQGMVPFVSTNLC 330
+G L GIPV+VK+ KG +T ML Y P D +V L+ +G + N+
Sbjct: 55 KGRLAGIPVAVKDNISTKGILTTCASKMLSN-YRPVF-DAHVVEKLKQEGAIIIGKANMD 112
Query: 331 QSGLSIESSNPIFGETLNPHNKTRTXXXXXXXXXXXXXXXXXLVGLGSDIGGSIRIPSSY 510
+ + + FG NPH++ R ++ LGSD GGSIR P+S+
Sbjct: 113 EFAMGTTTETSYFGVVRNPHDEARVAGGSSGGSGAVIAADEAVLSLGSDTGGSIRCPASF 172
Query: 511 CGVYGLKPTSQRFSSRHGKYLFPDQNIINVSVGPMARNIEDLVAVFKAMACGDLYHLDPY 690
CGVYGLKPT SR+G L P N + +GPMA +IEDL + + +A D
Sbjct: 173 CGVYGLKPT-YGLVSRYG--LIPYANSLE-QIGPMADSIEDLALLLEVIAGKDTRDSTNA 228
Query: 691 CPPLCFNDKIFDKKRPLRIGYYIHLGENQVTPVPAVVRAVQIAKKSLESAGHVLIEFNFP 870
F + R LR+G +G N + AV++ ++ H + E + P
Sbjct: 229 GREFRFE----PEDRKLRVGIIAEMGGND-DVMKRFNEAVEVIREK-----HEVGEVSMP 278
Query: 871 NIDYA 885
+ YA
Sbjct: 279 SFKYA 283
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 87,468,559
Number of Sequences: 369166
Number of extensions: 1602300
Number of successful extensions: 4155
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3915
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4042
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8838279920
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)