Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_005_D02
(628 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q14669|TRI12_HUMAN Thyroid receptor interacting protein ... 162 7e-40
sp|P33202|UFD4_YEAST Ubiquitin fusion degradation protein 4... 130 2e-30
sp|Q10435|YDE1_SCHPO Probable ubiquitin fusion degradation ... 127 2e-29
sp|Q8GY23|UPL1_ARATH E3 ubiquitin protein ligase UPL1 (Ubiq... 88 2e-17
sp|Q8H0T4|UPL2_ARATH E3 ubiquitin protein ligase UPL2 (Ubiq... 87 5e-17
sp|P53119|HUL5_YEAST Probable ubiquitin--protein ligase HUL5 84 4e-16
sp|Q9ULT8|HECD1_HUMAN HECT domain containing protein 1 84 4e-16
sp|Q05086|UBE3A_HUMAN Ubiquitin-protein ligase E3A (E6AP ub... 80 3e-15
sp|Q8C863|ITCH_MOUSE Itchy E3 ubiquitin protein ligase 78 2e-14
sp|Q96J02|ITCH_HUMAN Itchy homolog E3 ubiquitin protein lig... 76 6e-14
>sp|Q14669|TRI12_HUMAN Thyroid receptor interacting protein 12 (TRIP12)
Length = 1992
Score = 162 bits (410), Expect = 7e-40
Identities = 90/177 (50%), Positives = 111/177 (62%), Gaps = 1/177 (0%)
Frame = +3
Query: 3 LDLVANVTLIEGVRAQMECVIEGFDSLLPGARMHLSALFEAHXXXXXXXXXXXXDNQLWD 182
L LV L EGV Q + +GF+S+ P + HL + WD
Sbjct: 1822 LRLVIFWALNEGVSRQFDSFRDGFESVFPLS--HLQYFYPEELDQLLCGSKA----DTWD 1875
Query: 183 VKTLTEVCTCDHGYTMNSRSIVNLFEIMSEFTKEQRRHFVQFCTGTPRLPVGGFRALRPP 362
KTL E C DHGYT +SR++ LFEI+S F EQ+R F+QF TG+PRLPVGGFR+L PP
Sbjct: 1876 AKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPP 1935
Query: 363 FKIVNKK-EGGENPDNYLPSVMTCQNFLKLPDYSSKEVMRQRLLYALHEGQNAFFLS 530
IV K E ENPD++LPSVMTC N+LKLPDYSS E+MR++LL A EGQ +F LS
Sbjct: 1936 LTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS 1992
>sp|P33202|UFD4_YEAST Ubiquitin fusion degradation protein 4 (UB fusion protein 4)
Length = 1483
Score = 130 bits (328), Expect = 2e-30
Identities = 74/174 (42%), Positives = 100/174 (57%), Gaps = 1/174 (0%)
Frame = +3
Query: 12 VANVTLIEGVRAQMECVIEGFDSLLPGARMHLSALFEAHXXXXXXXXXXXXDNQLWDVKT 191
V + L +G+ Q++ IEGF + RM + LF + W + T
Sbjct: 1317 VIDQILGKGIEKQLKAFIEGFSKVFSYERMLI--LFPDELVDIFGRV-----EEDWSMAT 1369
Query: 192 LTEVCTCDHGYTMNSRSIVNLFEIMSEFTKEQRRHFVQFCTGTPRLPVGGFRALRPPFKI 371
L +HGYTM+S I + I+S F K +RR F+QF TG+P+LP+GGF++L P F +
Sbjct: 1370 LYTNLNAEHGYTMDSSIIHDFISIISAFGKHERRLFLQFLTGSPKLPIGGFKSLNPKFTV 1429
Query: 372 VNK-KEGGENPDNYLPSVMTCQNFLKLPDYSSKEVMRQRLLYALHEGQNAFFLS 530
V K E G D YLPSVMTC N+LKLP Y+SK++MR RL A+ EG AF LS
Sbjct: 1430 VLKHAEDGLTADEYLPSVMTCANYLKLPKYTSKDIMRSRLCQAIEEGAGAFLLS 1483
>sp|Q10435|YDE1_SCHPO Probable ubiquitin fusion degradation protein C12B10.01c
Length = 1647
Score = 127 bits (320), Expect = 2e-29
Identities = 71/177 (40%), Positives = 101/177 (57%), Gaps = 1/177 (0%)
Frame = +3
Query: 3 LDLVANVTLIEGVRAQMECVIEGFDSLLPGARMHLSALFEAHXXXXXXXXXXXXDNQLWD 182
L+ V + T+ +GV+ Q+E GF S+ P + + E ++ W
Sbjct: 1478 LNYVIDYTVGKGVQQQLEAFQNGFSSVFPYTSLQVLTEHELVTLFGTV-------DEDWS 1530
Query: 183 VKTLTEVCTCDHGYTMNSRSIVNLFEIMSEFTKEQRRHFVQFCTGTPRLPVGGFRALRPP 362
TL + DHGYTM S +I L +MS+ +++R F+QF TG+ +LP+GGF L PP
Sbjct: 1531 YATLMKSIVADHGYTMESPTIQRLLTLMSQMNFQEQRDFLQFITGSRKLPIGGFAGLNPP 1590
Query: 363 FKIVNK-KEGGENPDNYLPSVMTCQNFLKLPDYSSKEVMRQRLLYALHEGQNAFFLS 530
+V + E PD+YLPSVMTC N+LKLP+YSS EV+ RL A+ EGQ +F LS
Sbjct: 1591 LTVVRRLNEPPYVPDDYLPSVMTCVNYLKLPEYSSSEVLGSRLSKAILEGQGSFHLS 1647
>sp|Q8GY23|UPL1_ARATH E3 ubiquitin protein ligase UPL1 (Ubiquitin-protein ligase 1)
Length = 3684
Score = 87.8 bits (216), Expect = 2e-17
Identities = 62/177 (35%), Positives = 90/177 (50%), Gaps = 4/177 (2%)
Frame = +3
Query: 3 LDLVANVTLIEGVRAQMECVIEGFDSLLPGARMHLSALFEAHXXXXXXXXXXXXDNQLWD 182
+DLVA L +R Q+ +EGF+ L+P L ++F D D
Sbjct: 3514 VDLVAGHILTNAIRPQINAFLEGFNELIP---RELVSIFNDKELELLISGLPEIDFD--D 3568
Query: 183 VKTLTEVCTCDHGYTMNSRSIVNLFEIMSEFTKEQRRHFVQFCTGTPRLPVGGFRALR-- 356
+K TE + YT S I +E++ F+KE F+QF TGT ++P+ GF+AL+
Sbjct: 3569 LKANTEYTS----YTAGSPVIHWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKALQGI 3624
Query: 357 --PPFKIVNKKEGGENPDNYLPSVMTCQNFLKLPDYSSKEVMRQRLLYALHEGQNAF 521
P ++K G P+ LPS TC N L LP+Y SKE +++RLL A+HE F
Sbjct: 3625 SGPQRLQIHKAYGA--PER-LPSAHTCFNQLDLPEYQSKEQLQERLLLAIHEASEGF 3678
>sp|Q8H0T4|UPL2_ARATH E3 ubiquitin protein ligase UPL2 (Ubiquitin-protein ligase 2)
Length = 3658
Score = 86.7 bits (213), Expect = 5e-17
Identities = 61/177 (34%), Positives = 92/177 (51%), Gaps = 4/177 (2%)
Frame = +3
Query: 3 LDLVANVTLIEGVRAQMECVIEGFDSLLPGARMHLSALFEAHXXXXXXXXXXXXDNQLWD 182
+DLVA+ L +R Q+ +EG + L+P L ++F D D
Sbjct: 3491 VDLVADHILTSAIRPQINAFLEGLNELIP---RELVSIFNDKELELLISGLPEIDFD--D 3545
Query: 183 VKTLTEVCTCDHGYTMNSRSIVNLFEIMSEFTKEQRRHFVQFCTGTPRLPVGGFRALR-- 356
+K TE + YT+ S I +E++ F+KE F+QF TGT ++P+ GF+AL+
Sbjct: 3546 LKANTEYTS----YTVGSPVIRWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKALQGI 3601
Query: 357 --PPFKIVNKKEGGENPDNYLPSVMTCQNFLKLPDYSSKEVMRQRLLYALHEGQNAF 521
P ++K G +P+ LPS TC N L LP+Y SKE +++RLL A+HE F
Sbjct: 3602 SGPQRLQIHKAYG--SPER-LPSAHTCFNQLDLPEYQSKEQVQERLLLAIHEANEGF 3655
>sp|P53119|HUL5_YEAST Probable ubiquitin--protein ligase HUL5
Length = 910
Score = 83.6 bits (205), Expect = 4e-16
Identities = 44/111 (39%), Positives = 70/111 (63%), Gaps = 1/111 (0%)
Frame = +3
Query: 180 DVKTLTEVCTCDHGYTMNSRSIVNLFEIMSEFTKEQRRHFVQFCTGTPRLPVGGFRALRP 359
D+K+ TE GY ++IV+ +E+++EF E++ +F++F T P+ P+ GF+AL P
Sbjct: 803 DLKSNTEY----GGYKEEDQTIVDFWEVLNEFKFEEKLNFLKFVTSVPQAPLQGFKALDP 858
Query: 360 PFKIVNKKEGGENPDNY-LPSVMTCQNFLKLPDYSSKEVMRQRLLYALHEG 509
F I N + Y LP+ TC N LKLPDY +K ++R++LLYA++ G
Sbjct: 859 KFGIRN-----AGTEKYRLPTASTCVNLLKLPDYRNKTILREKLLYAINSG 904
>sp|Q9ULT8|HECD1_HUMAN HECT domain containing protein 1
Length = 1620
Score = 83.6 bits (205), Expect = 4e-16
Identities = 50/168 (29%), Positives = 82/168 (48%)
Frame = +3
Query: 3 LDLVANVTLIEGVRAQMECVIEGFDSLLPGARMHLSALFEAHXXXXXXXXXXXXDNQLWD 182
+DL+ + + G++ QME +GF+ + P ++ + E + W
Sbjct: 1456 VDLMFDFCMHTGIQKQMEAFRDGFNKVFPMEKLSSFSHEEVQMILCGN------QSPSWA 1509
Query: 183 VKTLTEVCTCDHGYTMNSRSIVNLFEIMSEFTKEQRRHFVQFCTGTPRLPVGGFRALRPP 362
+ + GYT +S + ++ + ++R+ F+QF TG LP GG L P
Sbjct: 1510 AEDIINYTEPKLGYTRDSPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPR 1569
Query: 363 FKIVNKKEGGENPDNYLPSVMTCQNFLKLPDYSSKEVMRQRLLYALHE 506
+V K + D PSV TC ++LKLP+YSS+E+MR+RLL A E
Sbjct: 1570 LTVVRKVDA---TDASYPSVNTCVHYLKLPEYSSEEIMRERLLAATME 1614
>sp|Q05086|UBE3A_HUMAN Ubiquitin-protein ligase E3A (E6AP ubiquitin-protein ligase)
(Oncogenic protein-associated protein E6-AP) (Human
papillomavirus E6-associated protein)
Length = 875
Score = 80.5 bits (197), Expect = 3e-15
Identities = 48/107 (44%), Positives = 63/107 (58%)
Frame = +3
Query: 180 DVKTLTEVCTCDHGYTMNSRSIVNLFEIMSEFTKEQRRHFVQFCTGTPRLPVGGFRALRP 359
D + L E D GYT +S I +EI+ FT EQ+R F+QF TGT R PVGG L
Sbjct: 766 DFQALEETTEYDGGYTRDSVLIREFWEIVHSFTDEQKRLFLQFTTGTDRAPVGGLGKL-- 823
Query: 360 PFKIVNKKEGGENPDNYLPSVMTCQNFLKLPDYSSKEVMRQRLLYAL 500
K++ K G + LP+ TC N L LP+YSSKE +++RLL A+
Sbjct: 824 --KMIIAKNGPDT--ERLPTSHTCFNVLLLPEYSSKEKLKERLLKAI 866
>sp|Q8C863|ITCH_MOUSE Itchy E3 ubiquitin protein ligase
Length = 864
Score = 78.2 bits (191), Expect = 2e-14
Identities = 56/169 (33%), Positives = 78/169 (46%), Gaps = 3/169 (1%)
Frame = +3
Query: 9 LVANVTLIEGVRAQMECVIEGFDSLLPGARMHLSALFEAHXXXXXXXXXXXXDNQLWDVK 188
+VA L GV Q + EGF+ +LP + F+A D W
Sbjct: 701 MVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQY---FDAKELEVLLCGMQEIDLNDWQRH 757
Query: 189 TLTEVCTCDHGYTMNSRSIVNLFEIMSEFTKEQRRHFVQFCTGTPRLPVGGFRAL---RP 359
+ YT S+ I+ ++ + E E+R +QF TGT RLPVGGF L
Sbjct: 758 AIYR------HYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNG 811
Query: 360 PFKIVNKKEGGENPDNYLPSVMTCQNFLKLPDYSSKEVMRQRLLYALHE 506
P K +K G E N+LP TC N L LP Y S E ++++LL+A+ E
Sbjct: 812 PQKFCIEKVGKE---NWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEE 857
>sp|Q96J02|ITCH_HUMAN Itchy homolog E3 ubiquitin protein ligase (Itch)
(Atrophin-1-interacting protein 4) (AIP4)
(NFE2-associated polypeptide 1) (NAPP1)
Length = 903
Score = 76.3 bits (186), Expect = 6e-14
Identities = 55/169 (32%), Positives = 77/169 (45%), Gaps = 3/169 (1%)
Frame = +3
Query: 9 LVANVTLIEGVRAQMECVIEGFDSLLPGARMHLSALFEAHXXXXXXXXXXXXDNQLWDVK 188
+VA L GV Q + EGF+ +LP + F+A D W
Sbjct: 740 MVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQY---FDAKELEVLLCGMQEIDLNDWQRH 796
Query: 189 TLTEVCTCDHGYTMNSRSIVNLFEIMSEFTKEQRRHFVQFCTGTPRLPVGGFRAL---RP 359
+ Y S+ I+ ++ + E E+R +QF TGT RLPVGGF L
Sbjct: 797 AIYR------HYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNG 850
Query: 360 PFKIVNKKEGGENPDNYLPSVMTCQNFLKLPDYSSKEVMRQRLLYALHE 506
P K +K G E N+LP TC N L LP Y S E ++++LL+A+ E
Sbjct: 851 PQKFCIEKVGKE---NWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEE 896
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 68,679,448
Number of Sequences: 369166
Number of extensions: 1330456
Number of successful extensions: 3302
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3241
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 4974853140
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)