Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_005_A12
(731 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q61043|NIN_MOUSE Ninein 45 2e-04
sp|Q02224|CENPE_HUMAN Centromeric protein E (CENP-E protein) 43 8e-04
sp|P62135|RAD50_NANEQ DNA double-strand break repair rad50 ... 42 0.001
sp|P34549|YNJ5_CAEEL Hypothetical protein R10E11.5 in chrom... 42 0.001
sp|P38748|YHB0_YEAST Hypothetical 67.5 kDa protein in PRPS4... 42 0.002
sp|P40457|MLP2_YEAST Protein MLP2 (Myosin-like protein 2) 42 0.002
sp|P25386|USO1_YEAST Intracellular protein transport protei... 42 0.002
sp|P08106|HSP70_CHICK Heat shock 70 kDa protein (HSP70) 41 0.003
sp|Q91VW5|GOGA4_MOUSE Golgin subfamily A member 4 (tGolgin-1) 41 0.004
sp|P17536|TPM1_YEAST Tropomyosin 1 40 0.006
>sp|Q61043|NIN_MOUSE Ninein
Length = 2035
Score = 45.1 bits (105), Expect = 2e-04
Identities = 33/164 (20%), Positives = 82/164 (50%), Gaps = 3/164 (1%)
Frame = +1
Query: 43 VEKDDSIIDTNENAKKRQDEFDRFEYNLSKYIENPKENRSRRYRNEIQQINAEILKEIQK 222
+E+ D ++ E + D+ ++ + ++ +++ RR +++++ E + I
Sbjct: 373 LERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAA 432
Query: 223 FKNDILQEKDQSLESNYKDLIEALQK-YKSKIKENCDETQQRLKRKIDNLID-QIIQDPN 396
KN++ QE++Q L+ K +E Q+ K+K +EN + L K +N ++ +++++
Sbjct: 433 LKNELRQEREQMLQQVGKQRVELEQEIQKAKTEENYIRDRLALSLKENNRLETELLENAE 492
Query: 397 KL-NTDMVTSKLEECNKIIIGILQDAWAKQYKELANFLKSQDKL 525
KL + +T KL+ + + +L + + A F +++L
Sbjct: 493 KLAEYESLTQKLQ---RSLENVLAEKFGDLDPSSAEFFLQEERL 533
>sp|Q02224|CENPE_HUMAN Centromeric protein E (CENP-E protein)
Length = 2663
Score = 43.1 bits (100), Expect = 8e-04
Identities = 34/139 (24%), Positives = 72/139 (51%), Gaps = 9/139 (6%)
Frame = +1
Query: 46 EKDDSIIDTNENAKKRQDEFDRFEYNLSKYIENPKENRSRRYRNEIQQINAEILKEIQKF 225
E++++I + N +++ E + L I + +N+ + + +Q+N + + E+Q+
Sbjct: 1488 EQEETINELRVNLSEKETEISTIQKQLEA-INDKLQNKIQEIYEKEEQLNIKQISEVQEN 1546
Query: 226 KNDILQEKD---------QSLESNYKDLIEALQKYKSKIKENCDETQQRLKRKIDNLIDQ 378
N++ Q K+ QS+ES +L LQ+ + +I+ E ++ +KR + L Q
Sbjct: 1547 VNELKQFKEHRKAKDSALQSIESKMLELTNRLQESQEEIQIMIKE-KEEMKRVQEAL--Q 1603
Query: 379 IIQDPNKLNTDMVTSKLEE 435
I +D K NT + +K++E
Sbjct: 1604 IERDQLKENTKEIVAKMKE 1622
Score = 38.9 bits (89), Expect = 0.014
Identities = 34/154 (22%), Positives = 75/154 (48%), Gaps = 4/154 (2%)
Frame = +1
Query: 46 EKDDSIIDTNENAKKRQDEFDRFEYNLS-KYIENPKENRSRRYRNEIQQINAEILKEIQK 222
EK+D I E ++ E + + + S + IE+PK+ + + E ++A+ +
Sbjct: 586 EKEDQIKKLQEYIDSQKLENIKMDLSYSLESIEDPKQMKQTLFDAETVALDAKRESAFLR 645
Query: 223 FKNDILQEKDQSLESNYKDLIEALQKYKSKIKENCDETQQRLKRKIDNLIDQIIQDPNKL 402
+N L+EK + L + YK + +Q Y+S++ E +++++ ++ + + KL
Sbjct: 646 SENLELKEKMKELATTYKQMENDIQLYQSQL-----EAKKKMQVDLEKELQSAFNEITKL 700
Query: 403 NTDMVTSKLEE---CNKIIIGILQDAWAKQYKEL 495
T ++ K+ + CN + G + D + KE+
Sbjct: 701 -TSLIDGKVPKDLLCNLELEGKITDLQKELNKEV 733
Score = 38.9 bits (89), Expect = 0.014
Identities = 54/251 (21%), Positives = 103/251 (41%), Gaps = 39/251 (15%)
Frame = +1
Query: 55 DSIIDTNENAKKRQDEFDRFEYNLSKYIENPKENRSRRYRNEIQQINAEIL---KEIQKF 225
D + T N K EF F K + E + + E +++N EI+ KE QKF
Sbjct: 803 DDLATTQSNYKSTDQEFQNF-----KTLHMDFEQKYKMVLEENERMNQEIVNLSKEAQKF 857
Query: 226 KNDI-------------LQEKDQSLESNYKDL-------------IEALQKYKSKIKENC 327
+ + LQEK + ++ ++ ++ +++ K+ I E
Sbjct: 858 DSSLGALKTELSYKTQELQEKTREVQERLNEMEQLKEQLENRDSPLQTVEREKTLITEKL 917
Query: 328 DETQQRLKRKIDNLID-QIIQDPNKLNTDMVTSKLEECNKIIIGI---LQDAWA--KQYK 489
+T + +K D + +Q+ ++ D + S + + + I L++A KQ++
Sbjct: 918 QQTLEEVKTLTQEKDDLKQLQESLQIERDQLKSDIHDTVNMNIDTQEQLRNALESLKQHQ 977
Query: 490 ELANFLKSQDKLRNSNSLH--QNTRPTIMNMWNPT--IIKPSDVPSGESNSVVAIIEKIP 657
E N LKS+ S +LH +NT T I K D+ + + ++ A ++
Sbjct: 978 ETINTLKSKISEEVSRNLHMEENTGETKDEFQQKMVGIDKKQDLEAKNTQTLTADVKDNE 1037
Query: 658 ILPVPNPMFQL 690
I+ +F L
Sbjct: 1038 IIEQQRKIFSL 1048
Score = 30.0 bits (66), Expect = 6.6
Identities = 21/125 (16%), Positives = 61/125 (48%), Gaps = 5/125 (4%)
Frame = +1
Query: 172 RNEIQQINAEILKEIQKFKNDILQEKDQSLESNYK-----DLIEALQKYKSKIKENCDET 336
+NE+QQ+ ++ E ++ K D+ + + ++E+ + D ++ Q+ ++ K + +
Sbjct: 1052 KNELQQMLESVIAEKEQLKTDLKENIEMTIENQEELRLLGDELKKQQEIVAQEKNHAIKK 1111
Query: 337 QQRLKRKIDNLIDQIIQDPNKLNTDMVTSKLEECNKIIIGILQDAWAKQYKELANFLKSQ 516
+ L R D L + +++ K + + K ++ + Q+ ++ K++ +
Sbjct: 1112 EGELSRTCDRLAE--VEEKLKEKSQQLQEKQQQLLNV-----QEEMSEMQKKINEIENLK 1164
Query: 517 DKLRN 531
++L+N
Sbjct: 1165 NELKN 1169
>sp|P62135|RAD50_NANEQ DNA double-strand break repair rad50 ATPase
Length = 786
Score = 42.4 bits (98), Expect = 0.001
Identities = 40/151 (26%), Positives = 75/151 (49%), Gaps = 4/151 (2%)
Frame = +1
Query: 82 AKKRQDEFDRFEYNLSKYIENPKENRSRRYRNEIQQINAEILKEIQKFKNDILQEKD-QS 258
+K +Q E R EY +K +E KE R Y +++ + KEI + +N I Q K+ +
Sbjct: 324 SKYKQYEEKRLEYEKAKMLEKEKEKAKREYSYLLKE-KESLEKEIAELQNKINQIKELEK 382
Query: 259 LESNYKDLIEALQKYKSKIK--ENCDETQQRLKRKI-DNLIDQIIQDPNKLNTDMVTSKL 429
+E ++ E + K+K+K EN + K+ + I+ + ++ KL + K
Sbjct: 383 MEQELLEIQERIGVIKAKLKLLENIKDRCPLCGAKLSSDTIEHLQKEREKLQKEYYYLK- 441
Query: 430 EECNKIIIGILQDAWAKQYKELANFLKSQDK 522
E+ N+++I I ++YK L L+++ K
Sbjct: 442 EKYNQLLIKIRA---LEKYKGLEKVLEAKTK 469
Score = 32.7 bits (73), Expect = 1.0
Identities = 25/98 (25%), Positives = 45/98 (45%), Gaps = 5/98 (5%)
Frame = +1
Query: 157 RSRRYRNEIQQINAEILKEIQKFKNDILQEKDQSLESNYKDLIEALQKYK-----SKIKE 321
R +N ++++ EI K+ K K + L+ +ES YK E +Y+ K KE
Sbjct: 294 RCNEIKNRLKELEKEI-KDYDKIKKEFLE-----IESKYKQYEEKRLEYEKAKMLEKEKE 347
Query: 322 NCDETQQRLKRKIDNLIDQIIQDPNKLNTDMVTSKLEE 435
L ++ ++L +I + NK+N K+E+
Sbjct: 348 KAKREYSYLLKEKESLEKEIAELQNKINQIKELEKMEQ 385
Score = 29.6 bits (65), Expect = 8.6
Identities = 36/139 (25%), Positives = 56/139 (40%), Gaps = 15/139 (10%)
Frame = +1
Query: 124 LSKYIENPKENRSRRYRNEIQQINAEILKEIQKFKNDILQEKDQSLESNYKDLIEALQKY 303
L K +E K N +++I I EI K E D+S+ YK L+ +
Sbjct: 460 LEKVLE-AKTKHLETIENRLKEIKPIIELEIPKI------ELDESIPYKYKQLLNKVSYL 512
Query: 304 KSKIKE-------NC--DETQQRLKRKIDNLIDQIIQDPNKLNTDMVTSKLEECNKI--- 447
++KIKE C D + LKR D I + + +++ +LE N +
Sbjct: 513 EAKIKEYERYKNIECPYDIAIEELKRIEDKYNKYIKKPALERELNLLLKELERYNSLLKE 572
Query: 448 ---IIGILQDAWAKQYKEL 495
I+ L +YKEL
Sbjct: 573 KEEILSKLNPEIETKYKEL 591
>sp|P34549|YNJ5_CAEEL Hypothetical protein R10E11.5 in chromosome III
Length = 529
Score = 42.4 bits (98), Expect = 0.001
Identities = 27/126 (21%), Positives = 63/126 (50%), Gaps = 17/126 (13%)
Frame = +1
Query: 199 EILKEIQKFKNDILQEKDQSLESNYKDLIEALQKYKSKIKENCDETQQRLK--------- 351
++L ++QK +D + + ++ ++ + ALQK + ++ + D+T + +K
Sbjct: 360 DLLAKVQKVADDTIDKSKTTVAADVAKMSGALQKAEEEVVQTIDQTVKNIKSNVNEVKKD 419
Query: 352 ------RKIDNLIDQIIQDPNKL--NTDMVTSKLEECNKIIIGILQDAWAKQYKELANFL 507
K+D++ ++ + L TD + SK++ ++ I L++A K K + + +
Sbjct: 420 VEKNIAEKVDDITKELEKSAKSLEETTDKIGSKIDNTSQAIKSNLEEASLKTEKSVNDAV 479
Query: 508 KSQDKL 525
KS +KL
Sbjct: 480 KSGEKL 485
>sp|P38748|YHB0_YEAST Hypothetical 67.5 kDa protein in PRPS4-STE20 intergenic region
Length = 585
Score = 42.0 bits (97), Expect = 0.002
Identities = 49/177 (27%), Positives = 80/177 (45%), Gaps = 20/177 (11%)
Frame = +1
Query: 67 DTNENAKKRQDEFDRFEY-NLSKYIENPKEN-----------RSRRYRNEIQQINAEILK 210
D + N DE Y N SK E N R R Y E Q+ L+
Sbjct: 378 DDDNNDIGNSDELQNVVYGNRSKNGEKSNSNKKDGELAANFLRHREYHLEYVQVLISQLE 437
Query: 211 EIQKFKNDILQEKDQ--SLESNYKDLIEALQKYKSKIKENCDETQQR---LKRKIDNLID 375
+++ LQEKDQ S SN + L ++++ K + + E Q+R K K++ D
Sbjct: 438 SQREYYELKLQEKDQTASDSSNVESLKKSMEDLKLQFQVTQKEWQKREMAQKSKLEE--D 495
Query: 376 QIIQDPNKLNTDMVTSK---LEECNKIIIGILQDAWAKQYKELANFLKSQDKLRNSN 537
++ + + N D ++ K LE NK + QD +Q K+L +L SQ+K ++++
Sbjct: 496 MLVIEGLQANLDHLSKKQEQLERENKALEESKQDL-EEQVKDLMFYLDSQEKFKDAD 551
>sp|P40457|MLP2_YEAST Protein MLP2 (Myosin-like protein 2)
Length = 1679
Score = 42.0 bits (97), Expect = 0.002
Identities = 48/215 (22%), Positives = 88/215 (40%), Gaps = 11/215 (5%)
Frame = +1
Query: 22 YEMFQIRVEKDDSIIDTNENAKKRQDEFDRFEYNLSKYIENPKENRSRRYRNEIQQINAE 201
Y +E + + A D F++ + N+ K + + R++ + ++Q+
Sbjct: 1358 YRTLMEEIESLKKELQIFKTANSSSDAFEKLKVNMEKEKDRIIDERTKEFEKKLQET--- 1414
Query: 202 ILKEIQKFKNDILQEKDQSLESNYKDLIEALQKYKSKIKENCDETQQRLKRKIDNLIDQI 381
K S E+ Y IE L+K +KE DET +R+K +NL +
Sbjct: 1415 -------------LNKSTSSEAEYSKDIETLKK--EWLKEYEDETLRRIKEAEENL-KKR 1458
Query: 382 IQDPNKLNTDMVTSKLEECNKIIIGILQDAWAKQYKELANFL----------KSQDKLRN 531
I+ P++ + SK +E L++ + K+ KE A L ++++L N
Sbjct: 1459 IRLPSEERIQKIISKRKE-------ELEEEFRKKLKENAGSLTFLDNKGSGEDAEEELWN 1511
Query: 532 SNSLHQNTRPT-IMNMWNPTIIKPSDVPSGESNSV 633
S S + RP+ + N +KP + N V
Sbjct: 1512 SPSKGNSERPSAVAGFINQKNLKPQEQLKNVKNDV 1546
Score = 31.6 bits (70), Expect = 2.3
Identities = 26/118 (22%), Positives = 63/118 (53%), Gaps = 2/118 (1%)
Frame = +1
Query: 61 IIDTNENAKKRQDEFDRFEYNLSKYIENPKENRSRRYRNEIQQINAEILKEIQKFKNDIL 240
IID ++ K + + + N + ++ +N + + R +Q+N + +EI + + D++
Sbjct: 1159 IIDEFKDITKEVTQVNILKEN-NAILQKSLKNVTEKNREIYKQLN-DRQEEISRLQRDLI 1216
Query: 241 QEKDQ-SLESNYKDLIEA-LQKYKSKIKENCDETQQRLKRKIDNLIDQIIQDPNKLNT 408
Q K+Q S+ SN + E+ +++ K + ++ + + K+ I+ L ++I KL++
Sbjct: 1217 QTKEQVSINSNKILVYESEMEQCKQRYQDLSQQQKDAQKKDIEKLTNEISDLKGKLSS 1274
>sp|P25386|USO1_YEAST Intracellular protein transport protein USO1 (Int-1)
Length = 1790
Score = 41.6 bits (96), Expect = 0.002
Identities = 42/157 (26%), Positives = 69/157 (43%), Gaps = 5/157 (3%)
Frame = +1
Query: 79 NAKKRQDEFDRFEYNLSKYIENPKENRSRRYRNEIQQINAEILKEIQKFKNDILQEKDQS 258
N + + E + L KY E N+ R+Y EI Q+N EI Q +N+ +++K+
Sbjct: 1146 NLESLEKEHEDLAAQLKKY-EEQIANKERQYNEEISQLNDEITSTQQ--ENESIKKKNDE 1202
Query: 259 LESNYKDLIEALQKYKSKIKENCDETQQRLKRKIDNLIDQIIQDPNKLNTDMVTSKLE-- 432
LE K + ++ E Q LK+ + ++ I++ K N S LE
Sbjct: 1203 LEGEVKAM------------KSTSEEQSNLKKSEIDALNLQIKELKKKNETNEASLLESI 1250
Query: 433 ---ECNKIIIGILQDAWAKQYKELANFLKSQDKLRNS 534
E + I LQD + KE++ +DKL+ S
Sbjct: 1251 KSVESETVKIKELQDECNFKEKEVSEL---EDKLKAS 1284
Score = 35.4 bits (80), Expect = 0.16
Identities = 38/158 (24%), Positives = 68/158 (43%), Gaps = 28/158 (17%)
Frame = +1
Query: 76 ENAKKRQDEFDRFEYNLSKYIENPKENRSRRYRNEIQQINAE-----------------I 204
EN K Q E D+ N K ++ KE+ + + EI+ IN I
Sbjct: 881 ENCKNLQKEKDKSNVNHQKETKSLKEDIAAKI-TEIKAINENLEEMKIQCNNLSKEKEHI 939
Query: 205 LKEIQKFKNDI---------LQEKDQSLESNYKDLIEALQKYKSKIKENCDETQQRLKRK 357
KE+ ++K+ L EK +SL +NYKD+ + ++E+ +E+ +L
Sbjct: 940 SKELVEYKSRFQSHDNLVAKLTEKLKSLANNYKDMQAENESLIKAVEESKNESSIQLS-N 998
Query: 358 IDNLIDQIIQDPN--KLNTDMVTSKLEECNKIIIGILQ 465
+ N ID + Q+ ++ + +E+ K I + Q
Sbjct: 999 LQNKIDSMSQEKENFQIERGSIEKNIEQLKKTISDLEQ 1036
Score = 34.3 bits (77), Expect = 0.35
Identities = 43/168 (25%), Positives = 70/168 (41%), Gaps = 38/168 (22%)
Frame = +1
Query: 52 DDSIIDT---NENAKKRQDEFDRFEYNLSKYIENPKENRSRRYRNEIQQIN--------- 195
+D I T NE+ KK+ DE + K +++ E +S ++EI +N
Sbjct: 1183 NDEITSTQQENESIKKKNDELE----GEVKAMKSTSEEQSNLKKSEIDALNLQIKELKKK 1238
Query: 196 -----AEILKEIQKFKNDILQEKDQSLESNYK--------DLIEA----------LQKYK 306
A +L+ I+ +++ ++ K+ E N+K D ++A LQK
Sbjct: 1239 NETNEASLLESIKSVESETVKIKELQDECNFKEKEVSELEDKLKASEDKNSKYLELQKES 1298
Query: 307 SKIKENCDETQQRLK---RKIDNLIDQIIQDPNKLNTDMVTSKLEECN 441
KIKE D LK KI NL + ++L+ TS E N
Sbjct: 1299 EKIKEELDAKTTELKIQLEKITNLSKAKEKSESELSRLKKTSSEERKN 1346
>sp|P08106|HSP70_CHICK Heat shock 70 kDa protein (HSP70)
Length = 634
Score = 41.2 bits (95), Expect = 0.003
Identities = 26/116 (22%), Positives = 53/116 (45%), Gaps = 3/116 (2%)
Frame = +1
Query: 49 KDDSIIDTNENAKKRQDEFDRFEYNLSKYIENPKENRSR---RYRNEIQQINAEILKEIQ 219
K++ I TN+ + +D+ DR KY + NR R + E N + E +
Sbjct: 500 KENKITITNDKGRLSKDDIDRMVQEAEKYKAEDEANRDRVGAKNSLESYTYNMKQTVEDE 559
Query: 220 KFKNDILQEKDQSLESNYKDLIEALQKYKSKIKENCDETQQRLKRKIDNLIDQIIQ 387
K K I + Q + +++I +L + + KE + Q+ L++ + ++ ++ Q
Sbjct: 560 KLKGKISDQDKQKVLDKCQEVISSLDRNQMAEKEEYEHKQKELEKLCNPIVTKLYQ 615
>sp|Q91VW5|GOGA4_MOUSE Golgin subfamily A member 4 (tGolgin-1)
Length = 2238
Score = 40.8 bits (94), Expect = 0.004
Identities = 24/99 (24%), Positives = 51/99 (51%)
Frame = +1
Query: 85 KKRQDEFDRFEYNLSKYIENPKENRSRRYRNEIQQINAEILKEIQKFKNDILQEKDQSLE 264
++ Q E D L + E E + ++ + +++ + LK++ + N L +K+Q LE
Sbjct: 1969 REHQQELDI----LKRECEQEAEEKLKQEQEDLELKHTSTLKQLMREFNTQLAQKEQELE 2024
Query: 265 SNYKDLIEALQKYKSKIKENCDETQQRLKRKIDNLIDQI 381
++ I+ Q+ ++++ E+ E Q+L RKI D +
Sbjct: 2025 RTVQETIDKAQEVEAELLESHQEETQQLHRKIAEKEDDL 2063
>sp|P17536|TPM1_YEAST Tropomyosin 1
Length = 199
Score = 40.0 bits (92), Expect = 0.006
Identities = 34/160 (21%), Positives = 76/160 (47%), Gaps = 20/160 (12%)
Frame = +1
Query: 25 EMFQIRVEKDDSIIDTNENAKKRQDEFDRFEYNLSKYIENPKEN------------RSRR 168
++ Q VEK++ I ++ +DE ++ E LS + ++N ++ +
Sbjct: 31 DLEQENVEKENQIKSLTVKNQQLEDEIEKLEAGLSDSKQTEQDNVEKENQIKSLTVKNHQ 90
Query: 169 YRNEIQQINAEILKEIQ--------KFKNDILQEKDQSLESNYKDLIEALQKYKSKIKEN 324
EI+++ AE+ + Q + ND +K+Q LE + ++ L++ K++E+
Sbjct: 91 LEEEIEKLEAELAESKQLSEDSHHLQSNNDNFSKKNQQLEEDLEESDTKLKETTEKLRES 150
Query: 325 CDETQQRLKRKIDNLIDQIIQDPNKLNTDMVTSKLEECNK 444
D +L+R++ L +Q ++ + + +T K E+ K
Sbjct: 151 -DLKADQLERRVAALEEQ--REEWERKNEELTVKYEDAKK 187
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.316 0.133 0.371
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 67,151,673
Number of Sequences: 369166
Number of extensions: 1196487
Number of successful extensions: 5979
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5538
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5925
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6534486000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)