Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_P21
(840 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P28812|Y3568_PSEAE Hypothetical protein PA3568 261 2e-69
sp|P55912|PRPE_SALTY Propionate--CoA ligase (Propionyl-CoA ... 176 7e-44
sp|P77495|PRPE_ECOLI Propionate--CoA ligase (Propionyl-CoA ... 175 1e-43
sp|Q758X0|ACS1_ASHGO Acetyl-coenzyme A synthetase 1 (Acetat... 157 3e-38
sp|Q01574|ACS1_YEAST Acetyl-coenzyme A synthetase 1 (Acetat... 156 6e-38
sp|O60011|ACS1_KLULA Acetyl-coenzyme A synthetase 1 (Acetat... 154 3e-37
sp|Q6FLU2|ACS1_CANGA Acetyl-coenzyme A synthetase 1 (Acetat... 152 1e-36
sp|Q8NJN3|ACS2_CANAL Acetyl-coenzyme A synthetase 2 (Acetat... 143 7e-34
sp|Q8KBY0|ACSA_CHLTE Acetyl-coenzyme A synthetase (Acetate-... 141 2e-33
sp|Q6BS00|ACS2_DEBHA Acetyl-coenzyme A synthetase 2 (Acetat... 139 1e-32
>sp|P28812|Y3568_PSEAE Hypothetical protein PA3568
Length = 628
Score = 261 bits (666), Expect = 2e-69
Identities = 133/275 (48%), Positives = 176/275 (64%)
Frame = +2
Query: 2 CTTIIYEGKPVGTPDSSQYFRVLNWHNPVSLFVAPTALRAIRREDPQAIHGVKYRHPRFR 181
CTT+ YEGKPV TPD+ Y+RV+ H SLF APTA+RAIR+EDP +Y R
Sbjct: 300 CTTVFYEGKPVRTPDAGAYWRVIEEHRVNSLFCAPTAIRAIRKEDPHGERVKRYDLGSLR 359
Query: 182 SLFLAGEHCDVDTLSWAKEIFKVPVLDHWWQTETGWPITCPAAGLETRADLVPPPGSAGR 361
LFLAGE D T W +E PV DHWWQTETGWP+T P G+ DL GS R
Sbjct: 360 HLFLAGEKLDSSTQHWLEEHTGRPVHDHWWQTETGWPVTAPCIGIAGH-DL--RAGSTNR 416
Query: 362 PVPGYDVKVLDTNTGVQLGPNQLGRIVVKLPMPPGMASTLYNNDEMFYKIFFTKFPGYYD 541
VPGY V+VLD + G LG N+ G IV+ LP+PPG A TL+ + + + + +PGYY
Sbjct: 417 AVPGYHVQVLD-DEGRPLGANRQGAIVIALPLPPGCAQTLWGDHPRYLQAYLGNYPGYYH 475
Query: 542 TMDAGMVTEFGHIMVMARDDDVLNIAGHRISSGALEEACLENPCVVECAVIGLDDQLKGQ 721
T D G + E G + +M R DDV+N++GHR+S+G +EE ++ V ECAVIG+ D+LKG
Sbjct: 476 TGDGGYLDEDGFVYIMGRTDDVINVSGHRLSTGEMEERVAQHQAVAECAVIGVRDELKGH 535
Query: 722 VPLALLVPDTDTIKAPEEIVSDTIKIVRDRIGAVA 826
VPL L+V D A E++ + + +VR++IGA+A
Sbjct: 536 VPLGLVVLKDDAGIAAEQLQRELVALVREQIGALA 570
>sp|P55912|PRPE_SALTY Propionate--CoA ligase (Propionyl-CoA synthetase)
Length = 628
Score = 176 bits (446), Expect = 7e-44
Identities = 97/261 (37%), Positives = 148/261 (56%), Gaps = 1/261 (0%)
Frame = +2
Query: 8 TIIYEGKPVGTPDSSQYFRVLNWHNPVSLFVAPTALRAIRREDPQAIHGVKYRHPRFRSL 187
TI+YEG P PD +++++ + +F APTA+R +++ I + +L
Sbjct: 303 TIVYEGLPT-YPDCGVWWKIVEKYQVNRMFSAPTAIRVLKKFPTAQIRN--HDLSSLEAL 359
Query: 188 FLAGEHCDVDTLSWAKEIFKVPVLDHWWQTETGWPITCPAAGLETRADLVPPPGSAGRPV 367
+LAGE D T SW E VPV+D++WQTE+GWPI A L+ R + GS G P+
Sbjct: 360 YLAGEPLDEPTASWVTETLGVPVIDNYWQTESGWPIMALARALDDRPSRL---GSPGVPM 416
Query: 368 PGYDVKVLDTNTGVQLGPNQLGRIVVKLPMPPGMASTLYNNDEMFYKIFFTKFPGY-YDT 544
GY+V++L+ TG G N+ G +V++ P+PPG T++ +D F K +++ F Y T
Sbjct: 417 YGYNVQLLNEVTGEPCGINEKGMLVIEGPLPPGCIQTIWGDDARFVKTYWSLFNRQVYAT 476
Query: 545 MDAGMVTEFGHIMVMARDDDVLNIAGHRISSGALEEACLENPCVVECAVIGLDDQLKGQV 724
D G+ G+ ++ R DDV+NIAGHR+ + +EE+ P V E AV+G+ D LKGQV
Sbjct: 477 FDWGIRDAEGYYFILGRTDDVINIAGHRLGTREIEESISSYPNVAEVAVVGIKDALKGQV 536
Query: 725 PLALLVPDTDTIKAPEEIVSD 787
+A ++P A E D
Sbjct: 537 AVAFVIPKQSDTLADREAARD 557
>sp|P77495|PRPE_ECOLI Propionate--CoA ligase (Propionyl-CoA synthetase)
Length = 628
Score = 175 bits (444), Expect = 1e-43
Identities = 96/254 (37%), Positives = 151/254 (59%), Gaps = 2/254 (0%)
Frame = +2
Query: 8 TIIYEGKPVGTPDSSQYFRVLNWHNPVSLFVAPTALRAIRREDPQAIHGVKYRHPRFRSL 187
TI+YEG P PD ++ ++ + +F APTA+R +++ I K+ L
Sbjct: 303 TIVYEGLPTW-PDCGVWWTIVEKYQVSRMFSAPTAIRVLKKFPTAEIR--KHDLSSLEVL 359
Query: 188 FLAGEHCDVDTLSWAKEIFKVPVLDHWWQTETGWPITCPAAGLETRADLVPPPGSAGRPV 367
+LAGE D T SW VPV+D++WQTE+GWPI A GL+ R + GS G P+
Sbjct: 360 YLAGEPLDEPTASWVSNTLDVPVIDNYWQTESGWPIMAIARGLDDRPTRL---GSPGVPM 416
Query: 368 PGYDVKVLDTNTGVQLGPNQLGRIVVKLPMPPGMASTLYNNDEMFYKIFFTKFPG-YYDT 544
GY+V++L+ TG G N+ G +VV+ P+PPG T++ +D F K +++ F Y T
Sbjct: 417 YGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGCIQTIWGDDGRFVKTYWSLFSRPVYAT 476
Query: 545 MDAGMVTEFGHIMVMARDDDVLNIAGHRISSGALEEACLENPCVVECAVIGLDDQLKGQV 724
D G+ G+ ++ R DDV+N+AGHR+ + +EE+ +P V E AV+G+ D LKGQV
Sbjct: 477 FDWGIRDADGYHFILGRTDDVINVAGHRLGTREIEESISSHPGVAEVAVVGVKDALKGQV 536
Query: 725 PLALLVP-DTDTIK 763
+A ++P ++D+++
Sbjct: 537 AVAFVIPKESDSLE 550
>sp|Q758X0|ACS1_ASHGO Acetyl-coenzyme A synthetase 1 (Acetate--CoA ligase 1)
(Acyl-activating enzyme 1)
Length = 694
Score = 157 bits (397), Expect = 3e-38
Identities = 98/285 (34%), Positives = 147/285 (51%), Gaps = 10/285 (3%)
Frame = +2
Query: 2 CTTIIYEGKPVGTPDSSQYFRVLNWHNPVSLFVAPTALRAIRREDPQAIHGVKYRHPRFR 181
CTT+++EG P P S+Y+ +++ ++ +VAPTALR ++R + G R R
Sbjct: 362 CTTVVFEGTPA-YPSYSRYWDIIDKYSVTQFYVAPTALRLLKRAGDSYVDGYSLR--TLR 418
Query: 182 SLFLAGEHCDVDTLSWAKEIF---KVPVLDHWWQTETGWPITCPAAGLETRADLVPPPGS 352
SL GE + W + ++PV+D +WQTE+G + P AG T PGS
Sbjct: 419 SLGTVGEPIAAEVWEWYYTVIGKREIPVIDTYWQTESGAHLVTPLAGGSTPMK----PGS 474
Query: 353 AGRPVPGYDVKVLDTNTGVQL-GPNQLGRIVVKLPMPPGMASTLYNNDEMFYKIFFTKFP 529
A P G D+ +LD TG +L GPN G + VK P P T++NN + + + +
Sbjct: 475 ASFPFFGIDLAILDPQTGEELLGPNVEGVLAVKQPWP-SFTRTIWNNHDRYLDTYLNPYK 533
Query: 530 GYYDTMDAGMVTEFGHIMVMARDDDVLNIAGHRISSGALEEACLENPCVVECAVIGLDDQ 709
GYY D G I ++ R DDV+N++GHR+S+ +E A ++ V ECAV+G D+
Sbjct: 534 GYYFAGDGAARDSQGFIWILGRVDDVVNVSGHRLSTAEVEAAIIQESMVAECAVVGFADE 593
Query: 710 LKGQVPLALLVP------DTDTIKAPEEIVSDTIKIVRDRIGAVA 826
L GQ A +V +T + + +EI I VR IG A
Sbjct: 594 LTGQAIAAFVVLKQKSSWNTASERELQEIKKHLILSVRRDIGPFA 638
>sp|Q01574|ACS1_YEAST Acetyl-coenzyme A synthetase 1 (Acetate--CoA ligase 1)
(Acyl-activating enzyme 1)
Length = 713
Score = 156 bits (395), Expect = 6e-38
Identities = 96/286 (33%), Positives = 145/286 (50%), Gaps = 11/286 (3%)
Frame = +2
Query: 2 CTTIIYEGKPVGTPDSSQYFRVLNWHNPVSLFVAPTALRAIRREDPQAI--HGVKYRHPR 175
C T+++EG P P+ S+Y+ +++ H +VAPTALR ++R I H +K
Sbjct: 381 CATLVFEGTPA-YPNYSRYWDIIDEHKVTQFYVAPTALRLLKRAGDSYIENHSLK----S 435
Query: 176 FRSLFLAGEHCDVDTLSWAKEIF---KVPVLDHWWQTETGWPITCPAAGLETRADLVPPP 346
R L GE + W E ++P++D +WQTE+G + P AG T P
Sbjct: 436 LRCLGSVGEPIAAEVWEWYSEKIGKNEIPIVDTYWQTESGSHLVTPLAGGVTPMK----P 491
Query: 347 GSAGRPVPGYDVKVLDTNTGVQLGPNQLGRIVVKLPMPPGMASTLYNNDEMFYKIFFTKF 526
GSA P G D VLD NTG +L + ++ P A T++ N + + + +
Sbjct: 492 GSASFPFFGIDAVVLDPNTGEELNTSHAEGVLAVKAAWPSFARTIWKNHDRYLDTYLNPY 551
Query: 527 PGYYDTMDAGMVTEFGHIMVMARDDDVLNIAGHRISSGALEEACLENPCVVECAVIGLDD 706
PGYY T D + G+I ++ R DDV+N++GHR+S+ +E A +E+P V ECAV+G +D
Sbjct: 552 PGYYFTGDGAAKDKDGYIWILGRVDDVVNVSGHRLSTAEIEAAIIEDPIVAECAVVGFND 611
Query: 707 QLKGQVPLALLVP------DTDTIKAPEEIVSDTIKIVRDRIGAVA 826
L GQ A +V T T ++I + VR IG A
Sbjct: 612 DLTGQAVAAFVVLKNKSSWSTATDDELQDIKKHLVFTVRKDIGPFA 657
>sp|O60011|ACS1_KLULA Acetyl-coenzyme A synthetase 1 (Acetate--CoA ligase 1)
(Acyl-activating enzyme 1)
Length = 707
Score = 154 bits (389), Expect = 3e-37
Identities = 88/251 (35%), Positives = 137/251 (54%), Gaps = 4/251 (1%)
Frame = +2
Query: 2 CTTIIYEGKPVGTPDSSQYFRVLNWHNPVSLFVAPTALRAIRREDPQAIHGVKYRHPRFR 181
CTT+++EG P P+ S+Y+ +++ + +VAPTALR ++R G + R
Sbjct: 375 CTTLVFEGTPA-YPNFSRYWEIVDKYQVTQFYVAPTALRLLKRAGDSFTEGFSLKS--LR 431
Query: 182 SLFLAGEHCDVDTLSWAKEIF---KVPVLDHWWQTETGWPITCPAAGLETRADLVPPPGS 352
SL GE + W E ++P++D +WQTE+G + P AG T PG+
Sbjct: 432 SLGSVGEPIAAEVWEWYSEKIGKNELPIVDTYWQTESGSHLVTPLAGGATPMK----PGA 487
Query: 353 AGRPVPGYDVKVLDTNTGV-QLGPNQLGRIVVKLPMPPGMASTLYNNDEMFYKIFFTKFP 529
A P G D+ VLD TG+ Q G + G + +K P P A T++ N++ F + +P
Sbjct: 488 AAFPFFGIDLAVLDPTTGIEQTGEHAEGVLAIKRPWP-SFARTIWKNNDRFLDTYLKPYP 546
Query: 530 GYYDTMDAGMVTEFGHIMVMARDDDVLNIAGHRISSGALEEACLENPCVVECAVIGLDDQ 709
GYY T D + G ++ R DDV+N++GHR+S+ +E A +E+ V ECAV+G +D+
Sbjct: 547 GYYFTGDGVARDKDGFFWILGRVDDVVNVSGHRLSTAEIEAAIIEDDMVAECAVVGFNDE 606
Query: 710 LKGQVPLALLV 742
L GQ A +V
Sbjct: 607 LTGQAVAAFVV 617
>sp|Q6FLU2|ACS1_CANGA Acetyl-coenzyme A synthetase 1 (Acetate--CoA ligase 1)
(Acyl-activating enzyme 1)
Length = 704
Score = 152 bits (384), Expect = 1e-36
Identities = 97/285 (34%), Positives = 147/285 (51%), Gaps = 10/285 (3%)
Frame = +2
Query: 2 CTTIIYEGKPVGTPDSSQYFRVLNWHNPVSLFVAPTALRAIRREDPQAIHGVKYRHPRFR 181
CTT+++EG P P+ S+Y+ +++ H +VAPTALR ++R I G + R
Sbjct: 372 CTTLVFEGTPA-YPNFSRYWEIIDKHQVTQFYVAPTALRLLKRAGDSFIDGFSLKS--LR 428
Query: 182 SLFLAGEHCDVDTLSWAKEIF---KVPVLDHWWQTETGWPITCPAAGLETRADLVPPPGS 352
L GE + W + ++P++D +WQTE+G + P AG T PGS
Sbjct: 429 CLGSVGEPIAAEVWEWYSDKIGKNEIPIVDTYWQTESGSHLVTPLAGGVTPMK----PGS 484
Query: 353 AGRPVPGYDVKVLDTNTGVQLGPNQL-GRIVVKLPMPPGMASTLYNNDEMFYKIFFTKFP 529
A P G + +LD TG ++ + G + +K P P A T++ N + F + +
Sbjct: 485 ASLPFFGIETVILDPTTGEEINDSHAEGVLAIKRPWP-SFARTIWKNHDRFLDTYLNPYK 543
Query: 530 GYYDTMDAGMVTEFGHIMVMARDDDVLNIAGHRISSGALEEACLENPCVVECAVIGLDDQ 709
GYY T D + G+I ++ R DDV+N++GHR+S+ +E A +E+ V ECAV+G +D
Sbjct: 544 GYYFTGDGAARDKDGYIWILGRVDDVVNVSGHRLSTAEIEAAIIEDRMVAECAVVGFNDD 603
Query: 710 LKGQVPLALLV-PDTDT-IKAPEEIVSD----TIKIVRDRIGAVA 826
L GQ A +V D T A EE + D I VR IG A
Sbjct: 604 LTGQAVAAFVVLKDKSTWASASEEELQDIKKHLILTVRKDIGPFA 648
>sp|Q8NJN3|ACS2_CANAL Acetyl-coenzyme A synthetase 2 (Acetate--CoA ligase 2)
(Acyl-activating enzyme 2)
Length = 581
Score = 143 bits (360), Expect = 7e-34
Identities = 87/248 (35%), Positives = 127/248 (51%), Gaps = 4/248 (1%)
Frame = +2
Query: 8 TIIYEGKPVGTPDSSQYFRVLNWHNPVSLFVAPTALRAIRREDPQAIHGVKYRHPRFRSL 187
TII+EG P PD ++++++ H +VAPTALR +R+ Q I VKY R+L
Sbjct: 342 TIIFEGTPA-YPDYGRFWQIVEKHKATHFYVAPTALRLLRKAGEQEI--VKYDLSSLRTL 398
Query: 188 FLAGEHCDVDTLSWAKEIF---KVPVLDHWWQTETGWPITCPAAGLETRADLVP-PPGSA 355
GE D W E + + D +WQTE+G + P AG+ VP PGSA
Sbjct: 399 GSVGEPISPDIWEWYNEFVGKNQCHISDTYWQTESGSHLIAPLAGV------VPNKPGSA 452
Query: 356 GRPVPGYDVKVLDTNTGVQLGPNQLGRIVVKLPMPPGMASTLYNNDEMFYKIFFTKFPGY 535
P G D ++D TGV++ N ++ P MA T+Y N + + +PGY
Sbjct: 453 SYPFFGIDAALIDPVTGVEIEGNDAEGVLAIKDHWPSMARTVYKNHTKYMDTYMNPYPGY 512
Query: 536 YDTMDAGMVTEFGHIMVMARDDDVLNIAGHRISSGALEEACLENPCVVECAVIGLDDQLK 715
Y T D G+ + R DDV+N++GHR+S+ +E A +E+ V E AV+G+ D +
Sbjct: 513 YFTGDGAARDHDGYYWIRGRVDDVVNVSGHRLSTAEIEAALIEDKKVSEAAVVGIHDDIT 572
Query: 716 GQVPLALL 739
GQ L L
Sbjct: 573 GQAALLTL 580
>sp|Q8KBY0|ACSA_CHLTE Acetyl-coenzyme A synthetase (Acetate--CoA ligase) (Acyl-activating
enzyme)
Length = 659
Score = 141 bits (356), Expect = 2e-33
Identities = 91/277 (32%), Positives = 140/277 (50%), Gaps = 3/277 (1%)
Frame = +2
Query: 5 TTIIYEGKPVGTPDSSQYFRVLNWHNPVSLFVAPTALRAIRREDPQAIHGVKYRHPRFRS 184
T +YEG P P +++ ++N H L+ APTA+RA R + + K+ R
Sbjct: 338 TVFMYEGAP-NYPQWDRFWDIINRHKITILYTAPTAIRAFIRAGNEWV--TKHDLSSLRL 394
Query: 185 LFLAGEHCDVDTLSWAKEIF---KVPVLDHWWQTETGWPITCPAAGLETRADLVPPPGSA 355
L GE + + W + K P++D WWQTETG + P G PG+A
Sbjct: 395 LGTVGEPINPEAWMWYHKYVGQEKCPIVDTWWQTETGGIMISPMPGATPTK-----PGTA 449
Query: 356 GRPVPGYDVKVLDTNTGVQLGPNQLGRIVVKLPMPPGMASTLYNNDEMFYKIFFTKFPGY 535
RP+PG V V+ + G N+ G +VVK P P M T+Y ++E + K ++++FPG
Sbjct: 450 TRPLPGIMVDVVRKD-GTPCNANEGGYLVVKRPWP-SMLRTIYGDNERYEKTYWSEFPGM 507
Query: 536 YDTMDAGMVTEFGHIMVMARDDDVLNIAGHRISSGALEEACLENPCVVECAVIGLDDQLK 715
Y T D + G+I +M R DDV+N++GHR+ + +E A + + V E AV+ D++K
Sbjct: 508 YFTGDGARKDDDGYIWIMGRVDDVVNVSGHRLGTSEVESALVSHEAVAEAAVVSRPDEIK 567
Query: 716 GQVPLALLVPDTDTIKAPEEIVSDTIKIVRDRIGAVA 826
G L V D + ++ K V IGA+A
Sbjct: 568 GNA-LVAFVTLKDGYEGDAKLRDSLGKHVAKEIGAIA 603
>sp|Q6BS00|ACS2_DEBHA Acetyl-coenzyme A synthetase 2 (Acetate--CoA ligase 2)
(Acyl-activating enzyme 2)
Length = 679
Score = 139 bits (349), Expect = 1e-32
Identities = 90/290 (31%), Positives = 144/290 (49%), Gaps = 13/290 (4%)
Frame = +2
Query: 8 TIIYEGKPVGTPDSSQYFRVLNWHNPVSLFVAPTALRAIRREDPQAIHGVKYRHPRFRSL 187
T+++EG P PD + ++++ H +VAPTALR +R+ Q I KY R+L
Sbjct: 342 TVVFEGTPA-FPDYGRLWQIVEKHKATHFYVAPTALRLLRKSGEQEIE--KYDLSSLRTL 398
Query: 188 FLAGEHCDVDTLSWAKEIF---KVPVLDHWWQTETGWPITCPAAGLETRADLVP-PPGSA 355
GE D W E + V D +WQTE+G P AG+ VP PGSA
Sbjct: 399 GSVGEPISPDIWEWYNEKVGKGQCHVTDTYWQTESGSHFIAPIAGV------VPNKPGSA 452
Query: 356 GRPVPGYDVKVLDTNTGVQLGPNQLGRIVVKLPMPPGMASTLYNNDEMFYKIFFTKFPGY 535
P G ++D +G+++ N + ++ P MA ++Y N + + +PGY
Sbjct: 453 AVPFFGIQTCLIDPVSGIEIQGNDVEGVLAVKDTWPSMARSVYKNHTKYMDTYLNPYPGY 512
Query: 536 YDTMDAGMVTEFGHIMVMARDDDVLNIAGHRISSGALEEACLENPCVVECAVIGLDDQLK 715
Y T D G+ + R DDV+N++GHR+S+ +E A +E+ + E AV+G++D L
Sbjct: 513 YFTGDGAARDHDGYYWIRGRVDDVVNVSGHRLSTAEIEAALIEHNAISEAAVVGMNDDLT 572
Query: 716 GQVPLAL------LVPDTDTIKAPEE---IVSDTIKIVRDRIGAVAGMRN 838
GQ +A L+ + ++PEE + + I VR +IG A ++
Sbjct: 573 GQTVVAFVALKEHLIANLKADESPEEALKLKKEMILQVRTQIGPFAAPKS 622
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 106,963,475
Number of Sequences: 369166
Number of extensions: 2431249
Number of successful extensions: 6961
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6412
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6795
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8197207050
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)