Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_O21
(434 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q64442|DHSO_MOUSE Sorbitol dehydrogenase (L-iditol 2-deh... 108 4e-24
sp|P27867|DHSO_RAT Sorbitol dehydrogenase (L-iditol 2-dehyd... 103 1e-22
sp|Q00796|DHSO_HUMAN Sorbitol dehydrogenase (L-iditol 2-deh... 102 5e-22
sp|P07846|DHSO_SHEEP Sorbitol dehydrogenase (L-iditol 2-deh... 98 7e-21
sp|Q02912|DHSO_BOMMO Sorbitol dehydrogenase (L-iditol 2-deh... 76 3e-14
sp|Q98D10|XYLD_RHILO Putative D-xylulose reductase (Xylitol... 69 3e-12
sp|P39346|IDND_ECOLI L-idonate 5-dehydrogenase 67 2e-11
sp|P36624|DHSO_SCHPO Putative sorbitol dehydrogenase (L-idi... 66 4e-11
sp|P35497|DHSO1_YEAST Sorbitol dehydrogenase 1 (L-iditol 2-... 65 6e-11
sp|Q07786|DHSO2_YEAST Sorbitol dehydrogenase 2 (L-iditol 2-... 65 6e-11
>sp|Q64442|DHSO_MOUSE Sorbitol dehydrogenase (L-iditol 2-dehydrogenase)
Length = 375
Score = 108 bits (271), Expect = 4e-24
Identities = 54/107 (50%), Positives = 74/107 (69%)
Frame = +1
Query: 28 TIECSGAPNAMNLAIEATVSGGCVVIVGMGAPIVQAPLLSALIREVDIRGVFRYANCYSA 207
TIEC+GA +++ I AT SGG +VIVGMGA +V PL+ A IREVDI+GVFRY N +
Sbjct: 265 TIECTGAESSVQTGIYATHSGGTLVIVGMGAEMVNLPLVHAAIREVDIKGVFRYCNTWPM 324
Query: 208 ALSLISQKRINVSSLVTKIFPFSDSEKAFKMVKSGEGVKIMIEISKN 348
A+S+++ K +NV LVT FP + +AF+ K G G+K+MI+ N
Sbjct: 325 AISMLASKTLNVKPLVTHRFPLEKAVEAFETAKKGVGLKVMIKCDPN 371
>sp|P27867|DHSO_RAT Sorbitol dehydrogenase (L-iditol 2-dehydrogenase)
Length = 399
Score = 103 bits (258), Expect = 1e-22
Identities = 50/107 (46%), Positives = 73/107 (68%)
Frame = +1
Query: 28 TIECSGAPNAMNLAIEATVSGGCVVIVGMGAPIVQAPLLSALIREVDIRGVFRYANCYSA 207
TIEC+GA +++ I AT SGG +V+VGMG ++ PL+ A +REVDI+GVFRY N +
Sbjct: 289 TIECTGAESSVQDGIYATHSGGTLVVVGMGPEMINLPLVHAAVREVDIKGVFRYCNTWPM 348
Query: 208 ALSLISQKRINVSSLVTKIFPFSDSEKAFKMVKSGEGVKIMIEISKN 348
A+S+++ K +NV LVT FP + +AF+ K G G+K+MI+ N
Sbjct: 349 AVSMLASKTLNVKPLVTHRFPLEKAVEAFETAKKGLGLKVMIKCDPN 395
>sp|Q00796|DHSO_HUMAN Sorbitol dehydrogenase (L-iditol 2-dehydrogenase)
Length = 357
Score = 102 bits (253), Expect = 5e-22
Identities = 50/103 (48%), Positives = 71/103 (68%)
Frame = +1
Query: 28 TIECSGAPNAMNLAIEATVSGGCVVIVGMGAPIVQAPLLSALIREVDIRGVFRYANCYSA 207
TIEC+GA ++ I AT SGG +V+VG+G+ + PLL A IREVDI+GVFRY N +
Sbjct: 247 TIECTGAEASIQAGIYATRSGGTLVLVGLGSEMTTVPLLHAAIREVDIKGVFRYCNTWPV 306
Query: 208 ALSLISQKRINVSSLVTKIFPFSDSEKAFKMVKSGEGVKIMIE 336
A+S+++ K +NV LVT FP + +AF+ K G G+KIM++
Sbjct: 307 AISMLASKSVNVKPLVTHRFPLEKALEAFETFKKGLGLKIMLK 349
>sp|P07846|DHSO_SHEEP Sorbitol dehydrogenase (L-iditol 2-dehydrogenase)
Length = 354
Score = 98.2 bits (243), Expect = 7e-21
Identities = 47/103 (45%), Positives = 69/103 (66%)
Frame = +1
Query: 28 TIECSGAPNAMNLAIEATVSGGCVVIVGMGAPIVQAPLLSALIREVDIRGVFRYANCYSA 207
TIEC+G ++ I AT SGG +V+VG+G+ + PL+ A REVDI+GVFRY N +
Sbjct: 244 TIECTGVETSIQAGIYATHSGGTLVLVGLGSEMTSVPLVHAATREVDIKGVFRYCNTWPM 303
Query: 208 ALSLISQKRINVSSLVTKIFPFSDSEKAFKMVKSGEGVKIMIE 336
A+S+++ K +NV LVT FP + +AF+ K G G+K+MI+
Sbjct: 304 AISMLASKSVNVKPLVTHRFPLEKALEAFETSKKGLGLKVMIK 346
>sp|Q02912|DHSO_BOMMO Sorbitol dehydrogenase (L-iditol 2-dehydrogenase)
Length = 348
Score = 76.3 bits (186), Expect = 3e-14
Identities = 38/106 (35%), Positives = 62/106 (58%)
Frame = +1
Query: 22 DKTIECSGAPNAMNLAIEATVSGGCVVIVGMGAPIVQAPLLSALIREVDIRGVFRYANCY 201
D +I+ G +A +A+ T + G V++VG+ V+ PL AL+REVD+ G FR N Y
Sbjct: 241 DVSIDACGYGSAQRVALLVTKTAGLVLVVGIADKTVELPLSQALLREVDVVGSFRIMNTY 300
Query: 202 SAALSLISQKRINVSSLVTKIFPFSDSEKAFKMVKSGEGVKIMIEI 339
AL+ +S I + +T FP + +++A + KSG +KI+I +
Sbjct: 301 QPALAAVSSGAIPLDKFITHRFPLNKTKEALDLAKSGAAMKILIHV 346
>sp|Q98D10|XYLD_RHILO Putative D-xylulose reductase (Xylitol dehydrogenase) (XDH)
Length = 348
Score = 69.3 bits (168), Expect = 3e-12
Identities = 40/106 (37%), Positives = 59/106 (55%), Gaps = 2/106 (1%)
Frame = +1
Query: 22 DKTIECSGAPNAMNLAIEATVSGGCVVIVGMGAPIVQAPLLSALIREVDIRGVFRYANCY 201
D E SG+P A + GG VV+VG+ V + +A+ +EV I VFRYAN +
Sbjct: 236 DIVFEASGSPKAFADLFDVVRPGGAVVLVGLPVEPVALNVPAAISKEVRIETVFRYANIF 295
Query: 202 SAALSLISQKRINVSSLVTKIFPFSDSEKAFKMVKSG--EGVKIMI 333
AL LI+ ++++ L+T + F+DS KAF+ G E VK+ I
Sbjct: 296 DRALQLIASGKVDLKPLITGTYDFADSIKAFERAAQGNPEDVKLQI 341
>sp|P39346|IDND_ECOLI L-idonate 5-dehydrogenase
Length = 343
Score = 67.0 bits (162), Expect = 2e-11
Identities = 31/95 (32%), Positives = 60/95 (63%)
Frame = +1
Query: 13 GLVDKTIECSGAPNAMNLAIEATVSGGCVVIVGMGAPIVQAPLLSALIREVDIRGVFRYA 192
G D + E SG P+++N +E T + G +V VGMG + + P+++ + +E+ +RG FR+
Sbjct: 234 GYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPMMTLIGKEISLRGSFRFT 293
Query: 193 NCYSAALSLISQKRINVSSLVTKIFPFSDSEKAFK 297
+ ++ A+S ++ IN L++ +PF+D E+A +
Sbjct: 294 SEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALR 328
>sp|P36624|DHSO_SCHPO Putative sorbitol dehydrogenase (L-iditol 2-dehydrogenase) (Protein
tms1)
Length = 360
Score = 65.9 bits (159), Expect = 4e-11
Identities = 35/102 (34%), Positives = 57/102 (55%), Gaps = 1/102 (0%)
Frame = +1
Query: 13 GLVDKTIECSGAPNAMNLAIEATVSGGCVVIVGMGAPIVQAPLLSALIREVDIRGVFRYA 192
G D ++ +G ++ A+ A GG V G G P++ P+ + E+++ G FRYA
Sbjct: 246 GEFDFAVDATGVGICIHTAVLALKRGGTFVQAGNGKPVIDFPINHIINYEINVLGSFRYA 305
Query: 193 N-CYSAALSLISQKRINVSSLVTKIFPFSDSEKAFKMVKSGE 315
+ CY +L L+S ++V L+T F F D+ KA++ V SGE
Sbjct: 306 HGCYKQSLFLVSNGLVDVKPLITHRFAFKDALKAYETVASGE 347
>sp|P35497|DHSO1_YEAST Sorbitol dehydrogenase 1 (L-iditol 2-dehydrogenase 1)
Length = 357
Score = 65.1 bits (157), Expect = 6e-11
Identities = 38/107 (35%), Positives = 59/107 (55%), Gaps = 3/107 (2%)
Frame = +1
Query: 22 DKTIECSGAPNAMNLAIEATVSGGCVVIVGMGAPIVQAPLLSALIREVDIRGVFRYA-NC 198
D ECSGA ++ A++ T GG +V VGMG P+ +E+ + G FRY+
Sbjct: 247 DVVFECSGADVCIDAAVKTTKVGGTMVQVGMGKNYTNFPIAEVSGKEMKLIGCFRYSFGD 306
Query: 199 YSAALSLISQKRINVSSLVTKIFPFSDSEKA--FKMVKSGEGVKIMI 333
Y A++L++ ++NV L+T F F D+ KA + + GE VK +I
Sbjct: 307 YRDAVNLVATGKVNVKPLITHKFKFEDAAKAYDYNIAHGGEVVKTII 353
>sp|Q07786|DHSO2_YEAST Sorbitol dehydrogenase 2 (L-iditol 2-dehydrogenase 2)
Length = 357
Score = 65.1 bits (157), Expect = 6e-11
Identities = 38/107 (35%), Positives = 59/107 (55%), Gaps = 3/107 (2%)
Frame = +1
Query: 22 DKTIECSGAPNAMNLAIEATVSGGCVVIVGMGAPIVQAPLLSALIREVDIRGVFRYA-NC 198
D ECSGA ++ A++ T GG +V VGMG P+ +E+ + G FRY+
Sbjct: 247 DVVFECSGADVCIDAAVKTTKVGGTMVQVGMGKNYTNFPIAEVSGKEMKLIGCFRYSFGD 306
Query: 199 YSAALSLISQKRINVSSLVTKIFPFSDSEKA--FKMVKSGEGVKIMI 333
Y A++L++ ++NV L+T F F D+ KA + + GE VK +I
Sbjct: 307 YRDAVNLVATGKVNVKPLITHKFKFEDAAKAYDYNIAHGGEVVKTII 353
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 44,306,562
Number of Sequences: 369166
Number of extensions: 809136
Number of successful extensions: 2364
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2271
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2329
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2194785120
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)