Planarian EST Database


Dr_sW_004_O20

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_004_O20
         (401 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O35286|DHX15_MOUSE  Putative pre-mRNA splicing factor RNA...   202   2e-52
sp|O43143|DHX15_HUMAN  Putative pre-mRNA splicing factor RNA...   202   2e-52
sp|O22899|DHX15_ARATH  Putative pre-mRNA splicing factor ATP...   189   1e-48
sp|Q20875|DHX15_CAEEL  Putative pre-mRNA splicing factor ATP...   188   4e-48
sp|P53131|PRP43_YEAST  Pre-mRNA splicing factor RNA helicase...   140   7e-34
sp|O42945|DHX15_SCHPO  Probable pre-mRNA splicing factor RNA...   131   4e-31
sp|O60231|DHX16_HUMAN  Putative pre-mRNA splicing factor RNA...    92   5e-19
sp|Q7YR39|DHX16_PANTR  Putative pre-mRNA splicing factor RNA...    92   5e-19
sp|O45244|DHX16_CAEEL  Probable pre-mRNA splicing factor ATP...    89   4e-18
sp|Q10752|CDC28_SCHPO  Putative ATP-dependent RNA helicase c...    84   8e-17
>sp|O35286|DHX15_MOUSE Putative pre-mRNA splicing factor RNA helicase (DEAH box protein 15)
          Length = 795

 Score =  202 bits (515), Expect = 2e-52
 Identities = 91/118 (77%), Positives = 107/118 (90%)
 Frame = +2

Query: 2    NLRRSSTDFSSKDYYVNIRKALVSGFFMQVAHLERSGHYLTVKDNQIVHLHPSTCLDHKP 181
            NL R STDF+S+DYY+NIRKALV+G+FMQVAHLER+GHYLTVKDNQ+V LHPST LDHKP
Sbjct: 672  NLPRRSTDFTSRDYYINIRKALVTGYFMQVAHLERTGHYLTVKDNQVVQLHPSTVLDHKP 731

Query: 182  EWVLYNEFVLTTKNFIRTVCEVKPEWLVKVAPQYYDMSNFPPCAAKEIIERIINRMQN 355
            EWVLYNEFVLTTKN+IRT  ++KPEWLVK+APQYYDMSNFP C AK  ++RII ++Q+
Sbjct: 732  EWVLYNEFVLTTKNYIRTCTDIKPEWLVKIAPQYYDMSNFPQCEAKRQLDRIIAKLQS 789
>sp|O43143|DHX15_HUMAN Putative pre-mRNA splicing factor RNA helicase (DEAH box protein 15)
            (ATP-dependent RNA helicase #46)
          Length = 795

 Score =  202 bits (515), Expect = 2e-52
 Identities = 91/118 (77%), Positives = 107/118 (90%)
 Frame = +2

Query: 2    NLRRSSTDFSSKDYYVNIRKALVSGFFMQVAHLERSGHYLTVKDNQIVHLHPSTCLDHKP 181
            NL R STDF+S+DYY+NIRKALV+G+FMQVAHLER+GHYLTVKDNQ+V LHPST LDHKP
Sbjct: 672  NLPRRSTDFTSRDYYINIRKALVTGYFMQVAHLERTGHYLTVKDNQVVQLHPSTVLDHKP 731

Query: 182  EWVLYNEFVLTTKNFIRTVCEVKPEWLVKVAPQYYDMSNFPPCAAKEIIERIINRMQN 355
            EWVLYNEFVLTTKN+IRT  ++KPEWLVK+APQYYDMSNFP C AK  ++RII ++Q+
Sbjct: 732  EWVLYNEFVLTTKNYIRTCTDIKPEWLVKIAPQYYDMSNFPQCEAKRQLDRIIAKLQS 789
>sp|O22899|DHX15_ARATH Putative pre-mRNA splicing factor ATP-dependent RNA helicase
          Length = 729

 Score =  189 bits (481), Expect = 1e-48
 Identities = 82/120 (68%), Positives = 103/120 (85%)
 Frame = +2

Query: 2   NLRRSSTDFSSKDYYVNIRKALVSGFFMQVAHLERSGHYLTVKDNQIVHLHPSTCLDHKP 181
           NL+  STDF+S+DYYVNIRKA+++G+FMQVAHLER+GHYLTVKDNQ+VHLHPS CLDHKP
Sbjct: 603 NLKMCSTDFNSRDYYVNIRKAMLAGYFMQVAHLERTGHYLTVKDNQVVHLHPSNCLDHKP 662

Query: 182 EWVLYNEFVLTTKNFIRTVCEVKPEWLVKVAPQYYDMSNFPPCAAKEIIERIINRMQNGK 361
           EWV+YNE+VLTT+NFIRTV +++ EWLV VA  YYD+SNFP C AK  +E++  + +  K
Sbjct: 663 EWVIYNEYVLTTRNFIRTVTDIRGEWLVDVAQHYYDLSNFPNCEAKRALEKLYKKREREK 722
>sp|Q20875|DHX15_CAEEL Putative pre-mRNA splicing factor ATP-dependent RNA helicase
           F56D2.6
          Length = 739

 Score =  188 bits (477), Expect = 4e-48
 Identities = 85/117 (72%), Positives = 101/117 (86%)
 Frame = +2

Query: 2   NLRRSSTDFSSKDYYVNIRKALVSGFFMQVAHLERSGHYLTVKDNQIVHLHPSTCLDHKP 181
           NLRR STDF S+DYY+NIRKALV+GFFMQVAHLERSGHY+TVKDNQ+V+LHPST LDHKP
Sbjct: 616 NLRRVSTDFKSRDYYLNIRKALVAGFFMQVAHLERSGHYVTVKDNQLVNLHPSTVLDHKP 675

Query: 182 EWVLYNEFVLTTKNFIRTVCEVKPEWLVKVAPQYYDMSNFPPCAAKEIIERIINRMQ 352
           EW LYNEFVLTTKNFIRTV +V+PEWL+++APQYYD+ NFP    K  +  ++  +Q
Sbjct: 676 EWALYNEFVLTTKNFIRTVTDVRPEWLLQIAPQYYDLDNFPDGDTKRKLTTVMQTLQ 732
>sp|P53131|PRP43_YEAST Pre-mRNA splicing factor RNA helicase PRP43 (Helicase JA1)
          Length = 767

 Score =  140 bits (354), Expect = 7e-34
 Identities = 73/128 (57%), Positives = 92/128 (71%), Gaps = 8/128 (6%)
 Frame = +2

Query: 2    NLRRSSTDFSSKDYYVNIRKALVSGFFMQVAHLERSGH--YLTVKDNQIVHLHPSTCLDH 175
            NL  ++TD+ S  Y+ NIRKAL SGFFMQVA  +RSG   Y+TVKDNQ V +HPST L H
Sbjct: 631  NLELNTTDYESPKYFDNIRKALASGFFMQVAK-KRSGAKGYITVKDNQDVLIHPSTVLGH 689

Query: 176  KPEWVLYNEFVLTTKNFIRTVCEVKPEWLVKVAPQYYDMSNFPPCAAKEIIERI------ 337
              EWV+YNEFVLT+KN+IRTV  V+PEWL+++AP YYD+SNF     K  +ERI      
Sbjct: 690  DAEWVIYNEFVLTSKNYIRTVTSVRPEWLIEIAPAYYDLSNFQKGDVKLSLERIKEKVDR 749

Query: 338  INRMQNGK 361
            +N ++ GK
Sbjct: 750  LNELKQGK 757
>sp|O42945|DHX15_SCHPO Probable pre-mRNA splicing factor RNA helicase prp43
          Length = 735

 Score =  131 bits (330), Expect = 4e-31
 Identities = 61/96 (63%), Positives = 77/96 (80%), Gaps = 1/96 (1%)
 Frame = +2

Query: 17  STDFSSKDYYVNIRKALVSGFFMQVAHLERSG-HYLTVKDNQIVHLHPSTCLDHKPEWVL 193
           ST F  K+YYVNIR+ALVSGFFMQVA    +G +Y+T+KDNQ+V LHPS  L   PEWV+
Sbjct: 614 STPFDDKNYYVNIRRALVSGFFMQVAKKSANGKNYVTMKDNQVVSLHPSCGLSVTPEWVV 673

Query: 194 YNEFVLTTKNFIRTVCEVKPEWLVKVAPQYYDMSNF 301
           YNEFVLTTK+FIR V  ++PEWL+++AP YYD+ +F
Sbjct: 674 YNEFVLTTKSFIRNVTAIRPEWLIELAPNYYDLDDF 709
>sp|O60231|DHX16_HUMAN Putative pre-mRNA splicing factor RNA helicase (ATP-dependent RNA
            helicase #3) (DEAH-box protein 16)
          Length = 1041

 Score = 91.7 bits (226), Expect = 5e-19
 Identities = 44/99 (44%), Positives = 65/99 (65%), Gaps = 1/99 (1%)
 Frame = +2

Query: 44   YVNIRKALVSGFFMQVAHLERSGHYLTVKDNQIVHLHPSTCL-DHKPEWVLYNEFVLTTK 220
            Y+ +RKA+ +G+F   A L RSG Y TVK  Q V +HP++ L + +P W+LY+E VLTTK
Sbjct: 936  YIRVRKAITAGYFYHTARLTRSG-YRTVKQQQTVFIHPNSSLFEQQPRWLLYHELVLTTK 994

Query: 221  NFIRTVCEVKPEWLVKVAPQYYDMSNFPPCAAKEIIERI 337
             F+R V E++  WL++VAP YY         AK++ ++I
Sbjct: 995  EFMRQVLEIESSWLLEVAPHYYKAKELEDPHAKKMPKKI 1033
>sp|Q7YR39|DHX16_PANTR Putative pre-mRNA splicing factor RNA helicase (ATP-dependent RNA
            helicase #3) (DEAH-box protein 16)
          Length = 1044

 Score = 91.7 bits (226), Expect = 5e-19
 Identities = 44/99 (44%), Positives = 65/99 (65%), Gaps = 1/99 (1%)
 Frame = +2

Query: 44   YVNIRKALVSGFFMQVAHLERSGHYLTVKDNQIVHLHPSTCL-DHKPEWVLYNEFVLTTK 220
            Y+ +RKA+ +G+F   A L RSG Y TVK  Q V +HP++ L + +P W+LY+E VLTTK
Sbjct: 939  YIRVRKAITAGYFYHTARLTRSG-YRTVKQQQTVFIHPNSSLFEQQPRWLLYHELVLTTK 997

Query: 221  NFIRTVCEVKPEWLVKVAPQYYDMSNFPPCAAKEIIERI 337
             F+R V E++  WL++VAP YY         AK++ ++I
Sbjct: 998  EFMRQVLEIESSWLLEVAPHYYKAKELEDPHAKKMPKKI 1036
>sp|O45244|DHX16_CAEEL Probable pre-mRNA splicing factor ATP-dependent RNA helicase mog-4
            (Sex determination protein mog-4) (Masculinization of
            germ line protein 4)
          Length = 1008

 Score = 88.6 bits (218), Expect = 4e-18
 Identities = 40/95 (42%), Positives = 59/95 (62%), Gaps = 1/95 (1%)
 Frame = +2

Query: 5    LRRSSTDFSSKDYYVNIRKALVSGFFMQVAHLERSGHYLTVKDNQIVHLHPSTCL-DHKP 181
            L R   +  S    + IRKA+ +G+F  V+ L+ +GHY TVK     H HP++CL +  P
Sbjct: 888  LERVEIETKSSTDTIKIRKAITAGYFYNVSKLDNTGHYKTVKHKHTTHPHPNSCLFEETP 947

Query: 182  EWVLYNEFVLTTKNFIRTVCEVKPEWLVKVAPQYY 286
             WV+Y E V T+K F+R + E++  WL++VAP YY
Sbjct: 948  RWVVYFELVFTSKEFMREMSEIESGWLLEVAPHYY 982
>sp|Q10752|CDC28_SCHPO Putative ATP-dependent RNA helicase cdc28
          Length = 1055

 Score = 84.3 bits (207), Expect = 8e-17
 Identities = 38/84 (45%), Positives = 60/84 (71%), Gaps = 2/84 (2%)
 Frame = +2

Query: 53   IRKALVSGFFMQVAHLERSGH-YLTVKDNQIVHLHPSTCL-DHKPEWVLYNEFVLTTKNF 226
            I+KA+ +G+F   A L+RSG  Y TVK NQ V++HPS+ + + KP+ ++Y E VLTTK +
Sbjct: 959  IKKAITAGYFSNAARLDRSGDSYRTVKSNQTVYIHPSSSVAEKKPKVIIYFELVLTTKEY 1018

Query: 227  IRTVCEVKPEWLVKVAPQYYDMSN 298
             R + E++PEWL++++P Y+   N
Sbjct: 1019 CRQITEIQPEWLLEISPHYFKPEN 1042
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 51,498,049
Number of Sequences: 369166
Number of extensions: 1031557
Number of successful extensions: 2838
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2778
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2815
length of database: 68,354,980
effective HSP length: 98
effective length of database: 50,250,950
effective search space used: 1758783250
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)