Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_O12
(828 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O35075|DSCR3_MOUSE Down syndrome critical region protein... 230 3e-60
sp|O14972|DSCR3_HUMAN Down syndrome critical region protein... 222 9e-58
sp|Q5RF33|DSCR3_PONPY Down syndrome critical region protein... 219 6e-57
sp|Q8I4T1|VPS26_PLAF7 Vacuolar protein sorting 26 homolog (... 37 0.066
sp|O33123|LEU2_MYCLE 3-isopropylmalate dehydratase large su... 33 1.3
sp|P40336|VPS26_MOUSE Vacuolar protein sorting 26 (Vesicle ... 32 2.8
sp|O75436|VPS26_HUMAN Vacuolar protein sorting 26 (Vesicle ... 32 2.8
sp|O53237|LEU2_MYCTU 3-isopropylmalate dehydratase large su... 31 3.6
sp|Q7TXH6|LEU2_MYCBO 3-isopropylmalate dehydratase large su... 31 3.6
sp|Q9T091|VPS26_ARATH Vacuolar protein sorting 26 homolog (... 30 6.2
>sp|O35075|DSCR3_MOUSE Down syndrome critical region protein 3 homolog (Down syndrome
critical region protein A homolog)
Length = 297
Score = 230 bits (587), Expect = 3e-60
Identities = 115/255 (45%), Positives = 164/255 (64%)
Frame = +3
Query: 63 IEIRLQRINKTYIENEIIKGLVYIDSKQDFSHQGINLILSGMVNLEISSKTHGRFDAIYN 242
++I+++R NK Y E++ G+V I SK HQG++L + G VNL++S+K+ G F+A YN
Sbjct: 5 LDIKIKRANKVYHAGEMLSGVVVISSKDSVQHQGVSLTMEGTVNLQLSAKSVGVFEAFYN 64
Query: 243 SVKPIQLAQKTVELNKSGRIPSGVVEIVFEIPLQMNVSSTSGIKRLLETYRGVFINISYS 422
SVKPIQ+ T+++ K G+IPSG E+ FE PL + G K L ETY GVF+NI Y+
Sbjct: 65 SVKPIQIINSTIDVLKPGKIPSGKTEVPFEFPLLVK-----GSKVLYETYHGVFVNIQYT 119
Query: 423 LTVDIKRSILQKSLQAQCDILVQYQDIGHIDDKAAIKPMEFXXXXXXXXXXXXSPKLPNF 602
L D++RS+L K L C+ +V K P++F LP F
Sbjct: 120 LRCDMRRSLLAKDLTKTCEFIVHSAPQ---KGKLTPSPVDFTITPETLQNVKERASLPKF 176
Query: 603 SIVGKIDSSEISMKQPITGHLIVEHCDRPIKSIELQLVRVETCGCAEGYSKDSTEIENLQ 782
I G ++S+ ++ QP+TG L+VEH D I+SIELQLVRVETCGCAEGY++D+TEI+N+Q
Sbjct: 177 FIRGHLNSTNCAITQPLTGELVVEHSDAAIRSIELQLVRVETCGCAEGYARDATEIQNIQ 236
Query: 783 MGDGNVFRNFEIPLY 827
+ DG++ RN +PLY
Sbjct: 237 IADGDICRNLSVPLY 251
>sp|O14972|DSCR3_HUMAN Down syndrome critical region protein 3 (Down syndrome critical
region protein A)
Length = 297
Score = 222 bits (566), Expect = 9e-58
Identities = 112/255 (43%), Positives = 160/255 (62%)
Frame = +3
Query: 63 IEIRLQRINKTYIENEIIKGLVYIDSKQDFSHQGINLILSGMVNLEISSKTHGRFDAIYN 242
++I+++R NK Y E++ G+V I SK HQG++L + G VNL++S+K+ G F+A YN
Sbjct: 5 LDIKIKRANKVYHAGEVLSGVVVISSKDSVQHQGVSLTMEGTVNLQLSAKSVGVFEAFYN 64
Query: 243 SVKPIQLAQKTVELNKSGRIPSGVVEIVFEIPLQMNVSSTSGIKRLLETYRGVFINISYS 422
SVKPIQ+ T+E+ K G+ PSG EI FE PL + G K L ETY GVF+NI Y+
Sbjct: 65 SVKPIQIINSTIEMVKPGKFPSGKTEIPFEFPLHL-----KGNKVLYETYHGVFVNIQYT 119
Query: 423 LTVDIKRSILQKSLQAQCDILVQYQDIGHIDDKAAIKPMEFXXXXXXXXXXXXSPKLPNF 602
L D+KRS+L K L C+ +V K P++F LP F
Sbjct: 120 LRCDMKRSLLAKDLTKTCEFIVH---SAPQKGKFTPSPVDFTITPETLQNVKERALLPKF 176
Query: 603 SIVGKIDSSEISMKQPITGHLIVEHCDRPIKSIELQLVRVETCGCAEGYSKDSTEIENLQ 782
+ G ++S+ + QP+TG L+VE + I+S+ELQLVRVETCGCAEGY++D+TEI+N+Q
Sbjct: 177 LLRGHLNSTNCVITQPLTGELVVESSEAAIRSVELQLVRVETCGCAEGYARDATEIQNIQ 236
Query: 783 MGDGNVFRNFEIPLY 827
+ DG+V R +P+Y
Sbjct: 237 IADGDVCRGLSVPIY 251
>sp|Q5RF33|DSCR3_PONPY Down syndrome critical region protein 3 homolog
Length = 297
Score = 219 bits (559), Expect = 6e-57
Identities = 111/255 (43%), Positives = 159/255 (62%)
Frame = +3
Query: 63 IEIRLQRINKTYIENEIIKGLVYIDSKQDFSHQGINLILSGMVNLEISSKTHGRFDAIYN 242
++I+++R NK Y E++ G+V I SK HQG++L + G VNL++S+K+ G F+A YN
Sbjct: 5 LDIKIKRANKVYHAGEVLSGVVVISSKDSVQHQGVSLTMEGTVNLQLSAKSVGVFEAFYN 64
Query: 243 SVKPIQLAQKTVELNKSGRIPSGVVEIVFEIPLQMNVSSTSGIKRLLETYRGVFINISYS 422
SVKPIQ+ +E+ K G+ PSG EI FE PL + G K L ETY GVF+NI Y+
Sbjct: 65 SVKPIQIINSNIEMVKPGKFPSGKTEIPFEFPLHV-----KGNKVLYETYHGVFVNIQYT 119
Query: 423 LTVDIKRSILQKSLQAQCDILVQYQDIGHIDDKAAIKPMEFXXXXXXXXXXXXSPKLPNF 602
L D+KRS+L K L C+ +V K + P+ F LP F
Sbjct: 120 LRCDMKRSLLAKDLTKTCEFIVH---SAPQKGKFSPSPVHFTITPETLQNARERALLPKF 176
Query: 603 SIVGKIDSSEISMKQPITGHLIVEHCDRPIKSIELQLVRVETCGCAEGYSKDSTEIENLQ 782
+ G ++S+ + QP+TG L+VE + I+S+ELQLVRVETCGCAEGY++D+TEI+N+Q
Sbjct: 177 LLRGHLNSTNCVITQPLTGELVVESSEAAIRSVELQLVRVETCGCAEGYARDATEIQNIQ 236
Query: 783 MGDGNVFRNFEIPLY 827
+ DG+V R +P+Y
Sbjct: 237 IADGDVCRGLSVPIY 251
>sp|Q8I4T1|VPS26_PLAF7 Vacuolar protein sorting 26 homolog (VPS26 protein homolog)
Length = 297
Score = 37.0 bits (84), Expect = 0.066
Identities = 49/245 (20%), Positives = 93/245 (37%), Gaps = 2/245 (0%)
Frame = +3
Query: 96 YIENEIIKGLVYIDSK--QDFSHQGINLILSGMVNLEISSKTHGRFDAIYNSVKPIQLAQ 269
+ + E I G I K + F H GI L L G +N+ F +I ++P
Sbjct: 39 FSDGEDINGTATISLKPGKKFEHYGIKLELIGQINILNDKANSYDFFSISKDLEP----- 93
Query: 270 KTVELNKSGRIPSGVVEIVFEIPLQMNVSSTSGIKRLLETYRGVFINISYSLTVDIKRSI 449
P +VE + S + + E+Y G + + Y + ++I +
Sbjct: 94 -----------PGFLVES------KQFKWKFSAVDKQHESYFGTNVQLRYFVRLNIIKGY 136
Query: 450 LQKSLQAQCDILVQYQDIGHIDDKAAIKPMEFXXXXXXXXXXXXSPKLPNFSIVGKIDSS 629
++Q + D +VQ I P E I + D S
Sbjct: 137 -SGNIQKEIDFIVQNLCI----------PPEINNTIKMEVGIEDC-----LHIEFEYDKS 180
Query: 630 EISMKQPITGHLIVEHCDRPIKSIELQLVRVETCGCAEGYSKDSTEIENLQMGDGNVFRN 809
+ +K + G + IK +EL ++++ET G + Y+ ++ + ++ DG+ ++
Sbjct: 181 KYHLKDVVVGKVYFLLVRIKIKHMELDIIKMETSGVGKNYTTETVTLSKFEIMDGSPIKS 240
Query: 810 FEIPL 824
IP+
Sbjct: 241 ECIPV 245
>sp|O33123|LEU2_MYCLE 3-isopropylmalate dehydratase large subunit (Isopropylmalate
isomerase) (Alpha-IPM isomerase) (IPMI)
Length = 476
Score = 32.7 bits (73), Expect = 1.3
Identities = 35/166 (21%), Positives = 73/166 (43%), Gaps = 21/166 (12%)
Frame = +3
Query: 36 IEFQMAQPLIEIRLQRINKTYIE--------NEIIKGLVYIDSKQ-DFSHQGINLILSGM 188
I+ +A P+ I+++ + + E +I +G+V++ Q + G+ ++
Sbjct: 80 IDKPIADPVSRIQVETLRRNCAEFGVRLHPMGDIEQGIVHVVGPQLGLTQPGMTIVCG-- 137
Query: 189 VNLEISSKTHGRFDAIYNSVKPIQLAQ------------KTVELNKSGRIPSGVVEIVFE 332
+ + THG F AI + ++ KT+ + GR+P+GV
Sbjct: 138 ---DSHTSTHGAFGAIAMGIGTSEIEHVLATQTLPLRPFKTMAVTVDGRLPAGVTAKDII 194
Query: 333 IPLQMNVSSTSGIKRLLETYRGVFINISYSLTVDIKRSILQKSLQA 470
+ L + + G +LE YRG I S++++ + +I S++A
Sbjct: 195 LALIAKIGTGGGQGYVLE-YRGSVIE---SMSMEGRMTICNMSIEA 236
>sp|P40336|VPS26_MOUSE Vacuolar protein sorting 26 (Vesicle protein sorting 26) (H58
protein) (H beta 58)
Length = 327
Score = 31.6 bits (70), Expect = 2.8
Identities = 55/254 (21%), Positives = 95/254 (37%), Gaps = 6/254 (2%)
Frame = +3
Query: 81 RINKTYI--ENEIIKGLVYIDSKQD---FSHQGINLILSGMVNL-EISSKTHGRFDAIYN 242
++ K Y+ + E + G V + KQ HQGI + G + L S TH + +
Sbjct: 35 KVEKHYLFYDGESVSGKVNLAFKQPGKRLEHQGIRIEFVGQIELFNDKSNTHEFVNLVKE 94
Query: 243 SVKPIQLAQKTVELNKSGRIPSGVVEIVFEIPLQMNVSSTSGIKRLLETYRGVFINISYS 422
P +L Q FE +++ E+Y G + + Y
Sbjct: 95 LALPGELTQSR--------------SYDFEF---------MQVEKPYESYIGANVRLRYF 131
Query: 423 LTVDIKRSILQKSLQAQCDILVQYQDIGHIDDKAAIKPMEFXXXXXXXXXXXXSPKLPNF 602
L V I R + L + D++V +Q + D +IK ME
Sbjct: 132 LKVTIVRRLTD--LVKEYDLIV-HQLATYPDVNNSIK-MEVGIEDC-------------L 174
Query: 603 SIVGKIDSSEISMKQPITGHLIVEHCDRPIKSIELQLVRVETCGCAEGYSKDSTEIENLQ 782
I + + S+ +K I G + I+ +ELQL++ E G + ++ I +
Sbjct: 175 HIEFEYNKSKYHLKDVIVGKIYFLLVRIKIQHMELQLIKKEITGIGPSTTTETETIAKYE 234
Query: 783 MGDGNVFRNFEIPL 824
+ DG + IP+
Sbjct: 235 IMDGAPVKGESIPI 248
>sp|O75436|VPS26_HUMAN Vacuolar protein sorting 26 (Vesicle protein sorting 26) (hVPS26)
Length = 327
Score = 31.6 bits (70), Expect = 2.8
Identities = 55/254 (21%), Positives = 95/254 (37%), Gaps = 6/254 (2%)
Frame = +3
Query: 81 RINKTYI--ENEIIKGLVYIDSKQD---FSHQGINLILSGMVNL-EISSKTHGRFDAIYN 242
++ K Y+ + E + G V + KQ HQGI + G + L S TH + +
Sbjct: 35 KVEKHYLFYDGESVSGKVNLAFKQPGKRLEHQGIRIEFVGQIELFNDKSNTHEFVNLVKE 94
Query: 243 SVKPIQLAQKTVELNKSGRIPSGVVEIVFEIPLQMNVSSTSGIKRLLETYRGVFINISYS 422
P +L Q FE +++ E+Y G + + Y
Sbjct: 95 LALPGELTQSR--------------SYDFEF---------MQVEKPYESYIGANVRLRYF 131
Query: 423 LTVDIKRSILQKSLQAQCDILVQYQDIGHIDDKAAIKPMEFXXXXXXXXXXXXSPKLPNF 602
L V I R + L + D++V +Q + D +IK ME
Sbjct: 132 LKVTIVRRLTD--LVKEYDLIV-HQLATYPDVNNSIK-MEVGIEDC-------------L 174
Query: 603 SIVGKIDSSEISMKQPITGHLIVEHCDRPIKSIELQLVRVETCGCAEGYSKDSTEIENLQ 782
I + + S+ +K I G + I+ +ELQL++ E G + ++ I +
Sbjct: 175 HIEFEYNKSKYHLKDVIVGKIYFLLVRIKIQHMELQLIKKEITGIGPSTTTETETIAKYE 234
Query: 783 MGDGNVFRNFEIPL 824
+ DG + IP+
Sbjct: 235 IMDGAPVKGESIPI 248
>sp|O53237|LEU2_MYCTU 3-isopropylmalate dehydratase large subunit (Isopropylmalate
isomerase) (Alpha-IPM isomerase) (IPMI)
Length = 473
Score = 31.2 bits (69), Expect = 3.6
Identities = 34/166 (20%), Positives = 72/166 (43%), Gaps = 21/166 (12%)
Frame = +3
Query: 36 IEFQMAQPLIEIRLQRINKTYIE--------NEIIKGLVYIDSKQ-DFSHQGINLILSGM 188
I+ +A P+ +++ + + E +I +G+V++ Q + G+ ++
Sbjct: 77 IDQPIADPVSRTQVETLRRNCAEFGIRLHSMGDIEQGIVHVVGPQLGLTQPGMTIVCG-- 134
Query: 189 VNLEISSKTHGRFDAIYNSVKPIQLAQ------------KTVELNKSGRIPSGVVEIVFE 332
+ + THG F A+ + ++ KT+ +N GR+P GV
Sbjct: 135 ---DSHTSTHGAFGALAMGIGTSEVEHVLATQTLPLRPFKTMAVNVDGRLPDGVSAKDII 191
Query: 333 IPLQMNVSSTSGIKRLLETYRGVFINISYSLTVDIKRSILQKSLQA 470
+ L + + G ++E YRG I SL+++ + +I S++A
Sbjct: 192 LALIAKIGTGGGQGHVIE-YRGSAIE---SLSMEGRMTICNMSIEA 233
>sp|Q7TXH6|LEU2_MYCBO 3-isopropylmalate dehydratase large subunit (Isopropylmalate
isomerase) (Alpha-IPM isomerase) (IPMI)
Length = 473
Score = 31.2 bits (69), Expect = 3.6
Identities = 34/166 (20%), Positives = 72/166 (43%), Gaps = 21/166 (12%)
Frame = +3
Query: 36 IEFQMAQPLIEIRLQRINKTYIE--------NEIIKGLVYIDSKQ-DFSHQGINLILSGM 188
I+ +A P+ +++ + + E +I +G+V++ Q + G+ ++
Sbjct: 77 IDQPIADPVSRTQVETLRRNCAEFGIRLHSMGDIEQGIVHVVGPQLGLTQPGMTIVCG-- 134
Query: 189 VNLEISSKTHGRFDAIYNSVKPIQLAQ------------KTVELNKSGRIPSGVVEIVFE 332
+ + THG F A+ + ++ KT+ +N GR+P GV
Sbjct: 135 ---DSHTSTHGAFGALAMGIGTSEVEHVLATQTLPLRPFKTMAVNVDGRLPDGVSAKDII 191
Query: 333 IPLQMNVSSTSGIKRLLETYRGVFINISYSLTVDIKRSILQKSLQA 470
+ L + + G ++E YRG I SL+++ + +I S++A
Sbjct: 192 LALIAKIGTGGGQGHVIE-YRGSAIE---SLSMEGRMTICNMSIEA 233
>sp|Q9T091|VPS26_ARATH Vacuolar protein sorting 26 homolog (VPS26 protein homolog)
Length = 303
Score = 30.4 bits (67), Expect = 6.2
Identities = 32/147 (21%), Positives = 60/147 (40%)
Frame = +3
Query: 384 ETYRGVFINISYSLTVDIKRSILQKSLQAQCDILVQYQDIGHIDDKAAIKPMEFXXXXXX 563
ETY GV + + Y L V + R L+ Q ++ Y + I++ +IK ME
Sbjct: 118 ETYNGVNVRLRYVLKVTVTRGYAGSILEYQELVVRNYAPLPDINN--SIK-MEVGIEDC- 173
Query: 564 XXXXXXSPKLPNFSIVGKIDSSEISMKQPITGHLIVEHCDRPIKSIELQLVRVETCGCAE 743
I + + S+ +K I G + +K+++L++ R E+ G
Sbjct: 174 ------------LHIEFEYNKSKYHLKDVILGKIYFLLVRIKMKNMDLEIRRRESTGAGA 221
Query: 744 GYSKDSTEIENLQMGDGNVFRNFEIPL 824
++ + ++ DG R IP+
Sbjct: 222 NTHVETETLAKFELMDGTPVRGESIPV 248
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 90,634,827
Number of Sequences: 369166
Number of extensions: 1751090
Number of successful extensions: 4163
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4047
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4157
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8004331590
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)