Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_O11-1
(508 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q62217|SEM5A_MOUSE Semaphorin-5A precursor (Semaphorin F... 30 2.5
sp|Q7ZZV4|TSHB_ANGJA Thyrotropin beta chain precursor (Thyr... 30 2.5
sp|P98133|FBN1_BOVIN Fibrillin-1 precursor (MP340) 30 3.2
sp|Q92HI8|PPDK_RICCN Pyruvate, phosphate dikinase (Pyruvate... 30 3.2
sp|Q08127|TSHB_ANGAN Thyrotropin beta chain precursor (Thyr... 30 4.2
sp|P35555|FBN1_HUMAN Fibrillin-1 precursor 29 5.5
sp|Q09272|YQG1_CAEEL Hypothetical protein C41C4.1 in chromo... 29 5.5
sp|P10820|PERF_MOUSE Perforin 1 precursor (P1) (Lymphocyte ... 29 7.2
sp|Q01253|YSCL_YEREN Yop proteins translocation protein L 29 7.2
sp|Q13591|SEM5A_HUMAN Semaphorin-5A precursor (Semaphorin F... 29 7.2
>sp|Q62217|SEM5A_MOUSE Semaphorin-5A precursor (Semaphorin F) (Sema F)
Length = 1077
Score = 30.4 bits (67), Expect = 2.5
Identities = 30/124 (24%), Positives = 47/124 (37%), Gaps = 18/124 (14%)
Frame = +2
Query: 122 RCRWCSQAYPDRFQCENCVQTNNNCCFDPRNINIVSPALSICRQQRSCTFINLSXXXXXX 301
R R C +P QC + C FD I VS A S +++ C N+
Sbjct: 916 RARQCILLFPVGSQCSGNTTESRPCVFDSNFIPEVSVARSSSVEEKRCGEFNMFHMFHMM 975
Query: 302 XXG-----FGCINCVLNFQNC-----------CVSPINP--LNNYIAQNFCNFIDDQQ*L 427
G GC+ +L + C + P++P LN+ I N N +D +
Sbjct: 976 AVGLSSSILGCLLTLLVYTYCQRYQQQSHDATVIHPVSPAALNSSIT-NHINKLDKYDSV 1034
Query: 428 DFLK 439
+ +K
Sbjct: 1035 EAIK 1038
>sp|Q7ZZV4|TSHB_ANGJA Thyrotropin beta chain precursor (Thyroid-stimulating hormone beta
subunit) (TSH-beta) (TSH-B)
Length = 147
Score = 30.4 bits (67), Expect = 2.5
Identities = 16/48 (33%), Positives = 23/48 (47%), Gaps = 6/48 (12%)
Frame = +2
Query: 146 YPDRFQCENCVQTNNNCCF------DPRNINIVSPALSICRQQRSCTF 271
Y ++ +C+ CV N C DP N+V PA+ QR CT+
Sbjct: 30 YVEKPECDFCVAINTTICMGFCYSLDP---NVVGPAVKRLAVQRGCTY 74
>sp|P98133|FBN1_BOVIN Fibrillin-1 precursor (MP340)
Length = 2871
Score = 30.0 bits (66), Expect = 3.2
Identities = 29/114 (25%), Positives = 42/114 (36%), Gaps = 11/114 (9%)
Frame = +2
Query: 44 ACVEISETQLPETQNRFQQCPNYSMSRCRWCSQAY---PDRFQC---ENCVQTNNNC--- 196
AC++I+E +L +C N C+ Y PDR C + C N C
Sbjct: 1152 ACIDINECELSAHLCPHGRCVNLIGKYQCACNPGYHSTPDRLFCVDIDECSIMNGGCETF 1211
Query: 197 CFDPRNINIVS--PALSICRQQRSCTFINLSXXXXXXXXGFGCINCVLNFQNCC 352
C + S P ++ QRSCT I+ G C N ++ C
Sbjct: 1212 CTNSEGSYECSCQPGFALMPDQRSCTDIDECEDNPNICDGGQCTNIPGEYRCLC 1265
>sp|Q92HI8|PPDK_RICCN Pyruvate, phosphate dikinase (Pyruvate, orthophosphate dikinase)
Length = 882
Score = 30.0 bits (66), Expect = 3.2
Identities = 22/77 (28%), Positives = 38/77 (49%), Gaps = 1/77 (1%)
Frame = +3
Query: 48 VWKSVKRNCQKLKIDFNNAQTIQCLVADGVLKLIQTDFNVKIAFKLITIAV-LIHETLIL 224
V+K + + QKL+ + + Q I+ + + L ++QT + A I IAV ++ E LI
Sbjct: 312 VFKELSQIAQKLEEHYLDMQDIEFTIENNKLYILQTRTAKRTAIAAINIAVQMVEEKLIS 371
Query: 225 CHQLCQYVDNKEAVLLL 275
Q +D + LL
Sbjct: 372 KEQALMRIDPESLNQLL 388
>sp|Q08127|TSHB_ANGAN Thyrotropin beta chain precursor (Thyroid-stimulating hormone beta
subunit) (TSH-beta) (TSH-B)
Length = 147
Score = 29.6 bits (65), Expect = 4.2
Identities = 16/48 (33%), Positives = 23/48 (47%), Gaps = 6/48 (12%)
Frame = +2
Query: 146 YPDRFQCENCVQTNNNCCF------DPRNINIVSPALSICRQQRSCTF 271
Y ++ +C+ CV N C DP N+V PA+ QR CT+
Sbjct: 30 YVEKPECDFCVAINTTICMGFCYSLDP---NVVGPAVKRLVVQRGCTY 74
>sp|P35555|FBN1_HUMAN Fibrillin-1 precursor
Length = 2871
Score = 29.3 bits (64), Expect = 5.5
Identities = 29/114 (25%), Positives = 42/114 (36%), Gaps = 11/114 (9%)
Frame = +2
Query: 44 ACVEISETQLPETQNRFQQCPNYSMSRCRWCSQAY---PDRFQC---ENCVQTNNNC--- 196
AC++I+E +L +C N C+ Y PDR C + C N C
Sbjct: 1152 ACIDINECELSAHLCPNGRCVNLIGKYQCACNPGYHSTPDRLFCVDIDECSIMNGGCETF 1211
Query: 197 CFDPRNINIVS--PALSICRQQRSCTFINLSXXXXXXXXGFGCINCVLNFQNCC 352
C + S P ++ QRSCT I+ G C N ++ C
Sbjct: 1212 CTNSEGSYECSCQPGFALMPDQRSCTDIDECEDNPNICDGGQCTNIPGEYRCLC 1265
>sp|Q09272|YQG1_CAEEL Hypothetical protein C41C4.1 in chromosome II
Length = 329
Score = 29.3 bits (64), Expect = 5.5
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +2
Query: 107 NYSMSRCRWCSQAYPDRFQCENCVQTNNNC 196
++ ++C + +Q YPDR QC NC Q N C
Sbjct: 165 SFFSNKCIFEAQKYPDRKQCYNC-QGNVTC 193
>sp|P10820|PERF_MOUSE Perforin 1 precursor (P1) (Lymphocyte pore forming protein)
(Cytolysin)
Length = 554
Score = 28.9 bits (63), Expect = 7.2
Identities = 17/58 (29%), Positives = 26/58 (44%), Gaps = 12/58 (20%)
Frame = +2
Query: 62 ETQLPETQNRFQQCPNYSMSRCRWCSQAYPDR------------FQCENCVQTNNNCC 199
E Q P+ + Q +Y MSR RW + + P R +C++ TN +CC
Sbjct: 350 EEQNPKREALRQAISHYIMSRARWQNCSRPCRSGQHKSSHDSCQCECQDSKVTNQDCC 407
>sp|Q01253|YSCL_YEREN Yop proteins translocation protein L
Length = 223
Score = 28.9 bits (63), Expect = 7.2
Identities = 16/42 (38%), Positives = 23/42 (54%), Gaps = 2/42 (4%)
Frame = +3
Query: 195 AVLIHETLILCHQLCQYVDNK--EAVLLLTCHNVKDVDNRDL 314
A LIHET + C Q ++V+ + E VLL + D D D+
Sbjct: 85 ATLIHETQLQCQQFYRHVEQQMSEVVLLAVRKILNDYDQVDM 126
>sp|Q13591|SEM5A_HUMAN Semaphorin-5A precursor (Semaphorin F) (Sema F)
Length = 1074
Score = 28.9 bits (63), Expect = 7.2
Identities = 29/121 (23%), Positives = 45/121 (37%), Gaps = 15/121 (12%)
Frame = +2
Query: 122 RCRWCSQAYPDRFQCENCVQTNNNCCFDPRNINIVSPALSICRQQRSCTFINLSXXXXXX 301
R R C +P QC + C FD I VS A S +++ C N+
Sbjct: 916 RARQCILLFPMGSQCSGNTTESRPCVFDSNFIPEVSVARSSSVEEKRCGEFNMFHMIAVG 975
Query: 302 XXG--FGCINCVLNFQNC-----------CVSPIN--PLNNYIAQNFCNFIDDQQ*LDFL 436
GC+ +L + C + P++ PLN I N N +D ++ +
Sbjct: 976 LSSSILGCLLTLLVYTYCQRYQQQSHDATVIHPVSPAPLNTSIT-NHINKLDKYDSVEAI 1034
Query: 437 K 439
K
Sbjct: 1035 K 1035
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 55,449,442
Number of Sequences: 369166
Number of extensions: 1042135
Number of successful extensions: 2322
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2247
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2321
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3206272875
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)