Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_O04
(296 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P26269|ODPB_ASCSU Pyruvate dehydrogenase E1 component be... 127 6e-30
sp|P11177|ODPB_HUMAN Pyruvate dehydrogenase E1 component be... 125 4e-29
sp|Q9D051|ODPB_MOUSE Pyruvate dehydrogenase E1 component be... 120 7e-28
sp|P49432|ODPB_RAT Pyruvate dehydrogenase E1 component beta... 119 2e-27
sp|O44451|ODPB_CAEEL Pyruvate dehydrogenase E1 component be... 119 3e-27
sp|P32473|ODPB_YEAST Pyruvate dehydrogenase E1 component be... 109 2e-24
sp|O66113|ODPB_ZYMMO Pyruvate dehydrogenase E1 component, b... 105 4e-23
sp|Q9R9N4|ODPB_RHIME Pyruvate dehydrogenase E1 component, b... 97 1e-20
sp|Q38799|ODPB_ARATH Pyruvate dehydrogenase E1 component be... 94 7e-20
sp|Q09171|ODPB_SCHPO Pyruvate dehydrogenase E1 component be... 91 6e-19
>sp|P26269|ODPB_ASCSU Pyruvate dehydrogenase E1 component beta subunit, mitochondrial
precursor (PDHE1-B)
Length = 361
Score = 127 bits (320), Expect = 6e-30
Identities = 61/97 (62%), Positives = 75/97 (77%)
Frame = +3
Query: 3 EKTGTRVTLVSFSKGVDLCLNAANELAKIGVDCEGINLRSLRPLDMETVIKSVVKTHHLV 182
++ G +T+VS S GVD+ L+AA+ELAK G+DCE INLR +RPLD +TV SV+KT HLV
Sbjct: 232 QRPGKDITIVSLSIGVDVSLHAADELAKSGIDCEVINLRCVRPLDFQTVKDSVIKTKHLV 291
Query: 183 TVEESWPTCGIGSEILARVMETQAFNYLDAPALRVTG 293
TVE WP CG+G+EI ARV E+ AF YLD P LRVTG
Sbjct: 292 TVESGWPNCGVGAEISARVTESDAFGYLDGPILRVTG 328
>sp|P11177|ODPB_HUMAN Pyruvate dehydrogenase E1 component beta subunit, mitochondrial
precursor (PDHE1-B)
Length = 359
Score = 125 bits (313), Expect = 4e-29
Identities = 60/98 (61%), Positives = 78/98 (79%)
Frame = +3
Query: 3 EKTGTRVTLVSFSKGVDLCLNAANELAKIGVDCEGINLRSLRPLDMETVIKSVVKTHHLV 182
E+ GT +T+VS S+ V CL AA L+K GV+CE IN+R++RP+DMET+ SV+KT+HLV
Sbjct: 231 ERQGTHITVVSHSRPVGHCLEAAAVLSKEGVECEVINMRTIRPMDMETIEASVMKTNHLV 290
Query: 183 TVEESWPTCGIGSEILARVMETQAFNYLDAPALRVTGA 296
TVE WP G+G+EI AR+ME AFN+LDAPA+RVTGA
Sbjct: 291 TVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGA 328
>sp|Q9D051|ODPB_MOUSE Pyruvate dehydrogenase E1 component beta subunit, mitochondrial
precursor (PDHE1-B)
Length = 359
Score = 120 bits (302), Expect = 7e-28
Identities = 57/98 (58%), Positives = 77/98 (78%)
Frame = +3
Query: 3 EKTGTRVTLVSFSKGVDLCLNAANELAKIGVDCEGINLRSLRPLDMETVIKSVVKTHHLV 182
E+ GT +T+V+ S+ V CL AA L+K G++CE INLR++RP+D+E + SV+KT+HLV
Sbjct: 231 ERQGTHITVVAHSRPVGHCLEAAAVLSKEGIECEVINLRTIRPMDIEAIEASVMKTNHLV 290
Query: 183 TVEESWPTCGIGSEILARVMETQAFNYLDAPALRVTGA 296
TVE WP G+G+EI AR+ME AFN+LDAPA+RVTGA
Sbjct: 291 TVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGA 328
>sp|P49432|ODPB_RAT Pyruvate dehydrogenase E1 component beta subunit, mitochondrial
precursor (PDHE1-B)
Length = 359
Score = 119 bits (298), Expect = 2e-27
Identities = 56/98 (57%), Positives = 76/98 (77%)
Frame = +3
Query: 3 EKTGTRVTLVSFSKGVDLCLNAANELAKIGVDCEGINLRSLRPLDMETVIKSVVKTHHLV 182
E+ GT + +V +S+ V CL AA L+K G++CE INLR++RP+D+E + SV+KT+HLV
Sbjct: 231 ERQGTHINVVCYSRPVGHCLEAAAVLSKGGIECEVINLRTIRPMDIEAIEASVMKTNHLV 290
Query: 183 TVEESWPTCGIGSEILARVMETQAFNYLDAPALRVTGA 296
TVE WP G+G+EI AR+ME AFN+LDAPA+RVTGA
Sbjct: 291 TVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGA 328
>sp|O44451|ODPB_CAEEL Pyruvate dehydrogenase E1 component beta subunit, mitochondrial
precursor
Length = 352
Score = 119 bits (297), Expect = 3e-27
Identities = 59/97 (60%), Positives = 72/97 (74%)
Frame = +3
Query: 3 EKTGTRVTLVSFSKGVDLCLNAANELAKIGVDCEGINLRSLRPLDMETVIKSVVKTHHLV 182
E+ G VT+VS+S+GV+ L AA +L IGV E INLRSLRP D E++ +SV KTHHLV
Sbjct: 223 ERAGDHVTIVSYSRGVEFSLEAAKQLEAIGVSAEVINLRSLRPFDFESIRQSVHKTHHLV 282
Query: 183 TVEESWPTCGIGSEILARVMETQAFNYLDAPALRVTG 293
+VE WP GIGSEI A+VME+ F+ LDAP LRVTG
Sbjct: 283 SVETGWPFAGIGSEIAAQVMESDVFDQLDAPLLRVTG 319
>sp|P32473|ODPB_YEAST Pyruvate dehydrogenase E1 component beta subunit, mitochondrial
precursor (PDHE1-B)
Length = 366
Score = 109 bits (273), Expect = 2e-24
Identities = 54/99 (54%), Positives = 77/99 (77%), Gaps = 1/99 (1%)
Frame = +3
Query: 3 EKTGTRVTLVSFSKGVDLCLNAANELAK-IGVDCEGINLRSLRPLDMETVIKSVVKTHHL 179
E+ GT +++V++++ V L AA L K GV E INLRS+RPLD E +IK+V KT+HL
Sbjct: 236 EREGTDISIVTYTRNVQFSLEAAEILQKKYGVSAEVINLRSIRPLDTEAIIKTVKKTNHL 295
Query: 180 VTVEESWPTCGIGSEILARVMETQAFNYLDAPALRVTGA 296
+TVE ++P+ G+G+EI+A+VME++AF+YLDAP RVTGA
Sbjct: 296 ITVESTFPSFGVGAEIVAQVMESEAFDYLDAPIQRVTGA 334
>sp|O66113|ODPB_ZYMMO Pyruvate dehydrogenase E1 component, beta subunit
Length = 462
Score = 105 bits (261), Expect = 4e-23
Identities = 54/97 (55%), Positives = 69/97 (71%)
Frame = +3
Query: 6 KTGTRVTLVSFSKGVDLCLNAANELAKIGVDCEGINLRSLRPLDMETVIKSVVKTHHLVT 185
+ G VT+VS+S GV L AA LAK G+D E I+LR+LRPLD ET+++S+ KT+ +VT
Sbjct: 335 REGKDVTIVSYSIGVSFALTAAEALAKEGIDAEVIDLRTLRPLDKETILQSLAKTNRIVT 394
Query: 186 VEESWPTCGIGSEILARVMETQAFNYLDAPALRVTGA 296
VE+ WP C I SEI A ME + F+ LDAP LRVT A
Sbjct: 395 VEDGWPVCSISSEIAAIAME-EGFDNLDAPVLRVTNA 430
>sp|Q9R9N4|ODPB_RHIME Pyruvate dehydrogenase E1 component, beta subunit
Length = 460
Score = 96.7 bits (239), Expect = 1e-20
Identities = 51/96 (53%), Positives = 67/96 (69%)
Frame = +3
Query: 6 KTGTRVTLVSFSKGVDLCLNAANELAKIGVDCEGINLRSLRPLDMETVIKSVVKTHHLVT 185
+TG TLVSF G+ + AA EL G+D E I+LR++RP+D+ TVI+SV KT LVT
Sbjct: 334 RTGKDATLVSFGIGMTYAIKAAAELEAQGIDVEIIDLRTIRPMDLPTVIESVKKTGRLVT 393
Query: 186 VEESWPTCGIGSEILARVMETQAFNYLDAPALRVTG 293
VEE +P +G+EI RVM+ QAF+YLDAP L + G
Sbjct: 394 VEEGYPQSSVGTEIATRVMQ-QAFDYLDAPILTIAG 428
>sp|Q38799|ODPB_ARATH Pyruvate dehydrogenase E1 component beta subunit, mitochondrial
precursor (PDHE1-B)
Length = 363
Score = 94.4 bits (233), Expect = 7e-20
Identities = 52/98 (53%), Positives = 68/98 (69%)
Frame = +3
Query: 3 EKTGTRVTLVSFSKGVDLCLNAANELAKIGVDCEGINLRSLRPLDMETVIKSVVKTHHLV 182
E+ G VT+V+FSK V L AA +LA+ G+ E INLRS+RPLD T+ SV KT LV
Sbjct: 234 EREGKDVTIVTFSKMVGFALKAAEKLAEEGISAEVINLRSIRPLDRATINASVRKTSRLV 293
Query: 183 TVEESWPTCGIGSEILARVMETQAFNYLDAPALRVTGA 296
TVEE +P G+ +EI A V+E ++F+YLDAP R+ GA
Sbjct: 294 TVEEGFPQHGVCAEICASVVE-ESFSYLDAPVERIAGA 330
>sp|Q09171|ODPB_SCHPO Pyruvate dehydrogenase E1 component beta subunit, mitochondrial
precursor (PDHE1-B)
Length = 366
Score = 91.3 bits (225), Expect = 6e-19
Identities = 50/99 (50%), Positives = 70/99 (70%), Gaps = 1/99 (1%)
Frame = +3
Query: 3 EKTGTRVTLVSFSKGVDLCLNAANEL-AKIGVDCEGINLRSLRPLDMETVIKSVVKTHHL 179
E+ G +T+V S V L AA++L A GV+ E INLRS+RPLD+ T+ SV KT+ +
Sbjct: 236 ERPGKDITIVGESISVVTALEAADKLKADYGVEAEVINLRSIRPLDINTIAASVKKTNRI 295
Query: 180 VTVEESWPTCGIGSEILARVMETQAFNYLDAPALRVTGA 296
VTV++++ GIGSEI A++ME+ AF+YLDAP RV+ A
Sbjct: 296 VTVDQAYSQHGIGSEIAAQIMESDAFDYLDAPVERVSMA 334
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,301,604
Number of Sequences: 369166
Number of extensions: 693903
Number of successful extensions: 1737
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1705
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1727
length of database: 68,354,980
effective HSP length: 68
effective length of database: 55,793,000
effective search space used: 1673790000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)