Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_O03
(766 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P11177|ODPB_HUMAN Pyruvate dehydrogenase E1 component be... 248 1e-65
sp|P26269|ODPB_ASCSU Pyruvate dehydrogenase E1 component be... 246 5e-65
sp|Q9D051|ODPB_MOUSE Pyruvate dehydrogenase E1 component be... 240 3e-63
sp|O44451|ODPB_CAEEL Pyruvate dehydrogenase E1 component be... 239 5e-63
sp|P49432|ODPB_RAT Pyruvate dehydrogenase E1 component beta... 239 8e-63
sp|P32473|ODPB_YEAST Pyruvate dehydrogenase E1 component be... 223 3e-58
sp|Q38799|ODPB_ARATH Pyruvate dehydrogenase E1 component be... 213 6e-55
sp|Q09171|ODPB_SCHPO Pyruvate dehydrogenase E1 component be... 209 9e-54
sp|P52904|ODPB_PEA Pyruvate dehydrogenase E1 component beta... 201 1e-51
sp|Q9R9N4|ODPB_RHIME Pyruvate dehydrogenase E1 component, b... 201 2e-51
>sp|P11177|ODPB_HUMAN Pyruvate dehydrogenase E1 component beta subunit, mitochondrial
precursor (PDHE1-B)
Length = 359
Score = 248 bits (633), Expect = 1e-65
Identities = 120/188 (63%), Positives = 158/188 (84%)
Frame = +3
Query: 48 LKVLSPYSSEDARGLLKSAIRDPDPVIVLENEVLYGHPFDTPDEALSPDFLIPIGKAKIE 227
LKV+SP++SEDA+GL+KSAIRD +PV+VLENE++YG PF+ P EA S DFLIPIGKAKIE
Sbjct: 172 LKVVSPWNSEDAKGLIKSAIRDNNPVVVLENELMYGVPFEFPPEAQSKDFLIPIGKAKIE 231
Query: 228 KTGTSVTLVSFSKGVDLCLNAANELAKIGVDCEVINLRSLRPLDMETVIKSVVKTHHLVT 407
+ GT +T+VS S+ V CL AA L+K GV+CEVIN+R++RP+DMET+ SV+KT+HLVT
Sbjct: 232 RQGTHITVVSHSRPVGHCLEAAAVLSKEGVECEVINMRTIRPMDMETIEASVMKTNHLVT 291
Query: 408 VEESWPTCGIGSEILARVMETQAFNYLDAPALRVTGADIPMPYAKPLEMEALPRVENVIK 587
VE WP G+G+EI AR+ME AFN+LDAPA+RVTGAD+PMPYAK LE ++P+V+++I
Sbjct: 292 VEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPYAKILEDNSIPQVKDIIF 351
Query: 588 SVKKVLHI 611
++KK L+I
Sbjct: 352 AIKKTLNI 359
Score = 32.3 bits (72), Expect = 1.4
Identities = 11/16 (68%), Positives = 14/16 (87%)
Frame = -1
Query: 52 FSAWFTHCPALKVLSP 5
F+AW+ HCP LKV+SP
Sbjct: 162 FAAWYGHCPGLKVVSP 177
>sp|P26269|ODPB_ASCSU Pyruvate dehydrogenase E1 component beta subunit, mitochondrial
precursor (PDHE1-B)
Length = 361
Score = 246 bits (628), Expect = 5e-65
Identities = 118/189 (62%), Positives = 148/189 (78%)
Frame = +3
Query: 48 LKVLSPYSSEDARGLLKSAIRDPDPVIVLENEVLYGHPFDTPDEALSPDFLIPIGKAKIE 227
+KV+ PY EDARGLLK+A+RD +PVI LENE+LYG F EA SPDF++P G+AKI+
Sbjct: 173 VKVVVPYDCEDARGLLKAAVRDDNPVICLENEILYGMKFPVSPEAQSPDFVLPFGQAKIQ 232
Query: 228 KTGTSVTLVSFSKGVDLCLNAANELAKIGVDCEVINLRSLRPLDMETVIKSVVKTHHLVT 407
+ G +T+VS S GVD+ L+AA+ELAK G+DCEVINLR +RPLD +TV SV+KT HLVT
Sbjct: 233 RPGKDITIVSLSIGVDVSLHAADELAKSGIDCEVINLRCVRPLDFQTVKDSVIKTKHLVT 292
Query: 408 VEESWPTCGIGSEILARVMETQAFNYLDAPALRVTGADIPMPYAKPLEMEALPRVENVIK 587
VE WP CG+G+EI ARV E+ AF YLD P LRVTG D+PMPYA+PLE ALP+ +V+K
Sbjct: 293 VESGWPNCGVGAEISARVTESDAFGYLDGPILRVTGVDVPMPYAQPLETAALPQPADVVK 352
Query: 588 SVKKVLHIQ 614
VKK L++Q
Sbjct: 353 MVKKCLNVQ 361
Score = 30.4 bits (67), Expect = 5.5
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = -1
Query: 52 FSAWFTHCPALKVLSP 5
F+AWF HCP +KV+ P
Sbjct: 163 FTAWFMHCPGVKVVVP 178
>sp|Q9D051|ODPB_MOUSE Pyruvate dehydrogenase E1 component beta subunit, mitochondrial
precursor (PDHE1-B)
Length = 359
Score = 240 bits (612), Expect = 3e-63
Identities = 116/188 (61%), Positives = 156/188 (82%)
Frame = +3
Query: 48 LKVLSPYSSEDARGLLKSAIRDPDPVIVLENEVLYGHPFDTPDEALSPDFLIPIGKAKIE 227
LKV+SP++SEDA+GL+KSAIRD +PV++LENE++YG F+ P EA S DFLIPIGKAKIE
Sbjct: 172 LKVVSPWNSEDAKGLIKSAIRDNNPVVMLENELMYGVAFELPAEAQSKDFLIPIGKAKIE 231
Query: 228 KTGTSVTLVSFSKGVDLCLNAANELAKIGVDCEVINLRSLRPLDMETVIKSVVKTHHLVT 407
+ GT +T+V+ S+ V CL AA L+K G++CEVINLR++RP+D+E + SV+KT+HLVT
Sbjct: 232 RQGTHITVVAHSRPVGHCLEAAAVLSKEGIECEVINLRTIRPMDIEAIEASVMKTNHLVT 291
Query: 408 VEESWPTCGIGSEILARVMETQAFNYLDAPALRVTGADIPMPYAKPLEMEALPRVENVIK 587
VE WP G+G+EI AR+ME AFN+LDAPA+RVTGAD+PMPYAK LE ++P+V+++I
Sbjct: 292 VEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPYAKVLEDNSVPQVKDIIF 351
Query: 588 SVKKVLHI 611
+VKK L+I
Sbjct: 352 AVKKTLNI 359
Score = 32.3 bits (72), Expect = 1.4
Identities = 11/16 (68%), Positives = 14/16 (87%)
Frame = -1
Query: 52 FSAWFTHCPALKVLSP 5
F+AW+ HCP LKV+SP
Sbjct: 162 FAAWYGHCPGLKVVSP 177
>sp|O44451|ODPB_CAEEL Pyruvate dehydrogenase E1 component beta subunit, mitochondrial
precursor
Length = 352
Score = 239 bits (611), Expect = 5e-63
Identities = 118/188 (62%), Positives = 146/188 (77%)
Frame = +3
Query: 48 LKVLSPYSSEDARGLLKSAIRDPDPVIVLENEVLYGHPFDTPDEALSPDFLIPIGKAKIE 227
LKV+ PYS+EDA+GLLK+AIRD +PV+ LENE+LYG F DE LS DF++PIGKAKIE
Sbjct: 164 LKVVCPYSAEDAKGLLKAAIRDDNPVVFLENEILYGQSFPVGDEVLSDDFVVPIGKAKIE 223
Query: 228 KTGTSVTLVSFSKGVDLCLNAANELAKIGVDCEVINLRSLRPLDMETVIKSVVKTHHLVT 407
+ G VT+VS+S+GV+ L AA +L IGV EVINLRSLRP D E++ +SV KTHHLV+
Sbjct: 224 RAGDHVTIVSYSRGVEFSLEAAKQLEAIGVSAEVINLRSLRPFDFESIRQSVHKTHHLVS 283
Query: 408 VEESWPTCGIGSEILARVMETQAFNYLDAPALRVTGADIPMPYAKPLEMEALPRVENVIK 587
VE WP GIGSEI A+VME+ F+ LDAP LRVTG D+PMPY + LE ALP E+V+K
Sbjct: 284 VETGWPFAGIGSEIAAQVMESDVFDQLDAPLLRVTGVDVPMPYTQTLEAAALPTAEHVVK 343
Query: 588 SVKKVLHI 611
+VKK L+I
Sbjct: 344 AVKKSLNI 351
Score = 32.3 bits (72), Expect = 1.4
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = -1
Query: 52 FSAWFTHCPALKVLSP 5
FSAW+ HCP LKV+ P
Sbjct: 154 FSAWYAHCPGLKVVCP 169
>sp|P49432|ODPB_RAT Pyruvate dehydrogenase E1 component beta subunit, mitochondrial
precursor (PDHE1-B)
Length = 359
Score = 239 bits (609), Expect = 8e-63
Identities = 114/188 (60%), Positives = 155/188 (82%)
Frame = +3
Query: 48 LKVLSPYSSEDARGLLKSAIRDPDPVIVLENEVLYGHPFDTPDEALSPDFLIPIGKAKIE 227
LKV+SP++SEDA+GL+KSAIRD +PV++LENE++YG F+ P EA S DFLIPIGKAKIE
Sbjct: 172 LKVVSPWNSEDAKGLIKSAIRDDNPVVMLENELMYGVAFELPTEAQSKDFLIPIGKAKIE 231
Query: 228 KTGTSVTLVSFSKGVDLCLNAANELAKIGVDCEVINLRSLRPLDMETVIKSVVKTHHLVT 407
+ GT + +V +S+ V CL AA L+K G++CEVINLR++RP+D+E + SV+KT+HLVT
Sbjct: 232 RQGTHINVVCYSRPVGHCLEAAAVLSKGGIECEVINLRTIRPMDIEAIEASVMKTNHLVT 291
Query: 408 VEESWPTCGIGSEILARVMETQAFNYLDAPALRVTGADIPMPYAKPLEMEALPRVENVIK 587
VE WP G+G+EI AR+ME AFN+LDAPA+RVTGAD+PMPYAK LE ++P+V+++I
Sbjct: 292 VEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPYAKILEDNSIPQVKDIIF 351
Query: 588 SVKKVLHI 611
++KK L+I
Sbjct: 352 AIKKTLNI 359
Score = 32.3 bits (72), Expect = 1.4
Identities = 11/16 (68%), Positives = 14/16 (87%)
Frame = -1
Query: 52 FSAWFTHCPALKVLSP 5
F+AW+ HCP LKV+SP
Sbjct: 162 FAAWYGHCPGLKVVSP 177
>sp|P32473|ODPB_YEAST Pyruvate dehydrogenase E1 component beta subunit, mitochondrial
precursor (PDHE1-B)
Length = 366
Score = 223 bits (569), Expect = 3e-58
Identities = 112/190 (58%), Positives = 150/190 (78%), Gaps = 1/190 (0%)
Frame = +3
Query: 48 LKVLSPYSSEDARGLLKSAIRDPDPVIVLENEVLYGHPFDTPDEALSPDFLIPIGKAKIE 227
LKVL PYS+EDARGLLK+AIRDP+PV+ LENE+LYG F+ +EALSP+F +P KAKIE
Sbjct: 178 LKVLVPYSAEDARGLLKAAIRDPNPVVFLENELLYGESFEISEEALSPEFTLPY-KAKIE 236
Query: 228 KTGTSVTLVSFSKGVDLCLNAANELAK-IGVDCEVINLRSLRPLDMETVIKSVVKTHHLV 404
+ GT +++V++++ V L AA L K GV EVINLRS+RPLD E +IK+V KT+HL+
Sbjct: 237 REGTDISIVTYTRNVQFSLEAAEILQKKYGVSAEVINLRSIRPLDTEAIIKTVKKTNHLI 296
Query: 405 TVEESWPTCGIGSEILARVMETQAFNYLDAPALRVTGADIPMPYAKPLEMEALPRVENVI 584
TVE ++P+ G+G+EI+A+VME++AF+YLDAP RVTGAD+P PYAK LE A P ++
Sbjct: 297 TVESTFPSFGVGAEIVAQVMESEAFDYLDAPIQRVTGADVPTPYAKELEDFAFPDTPTIV 356
Query: 585 KSVKKVLHIQ 614
K+VK+VL I+
Sbjct: 357 KAVKEVLSIE 366
>sp|Q38799|ODPB_ARATH Pyruvate dehydrogenase E1 component beta subunit, mitochondrial
precursor (PDHE1-B)
Length = 363
Score = 213 bits (541), Expect = 6e-55
Identities = 108/187 (57%), Positives = 141/187 (75%)
Frame = +3
Query: 48 LKVLSPYSSEDARGLLKSAIRDPDPVIVLENEVLYGHPFDTPDEALSPDFLIPIGKAKIE 227
LKVL+PYS+EDARGLLK+AIRDPDPV+ LENE+LYG F +EAL F +PIGKAKIE
Sbjct: 175 LKVLAPYSAEDARGLLKAAIRDPDPVVFLENELLYGESFPISEEALDSSFCLPIGKAKIE 234
Query: 228 KTGTSVTLVSFSKGVDLCLNAANELAKIGVDCEVINLRSLRPLDMETVIKSVVKTHHLVT 407
+ G VT+V+FSK V L AA +LA+ G+ EVINLRS+RPLD T+ SV KT LVT
Sbjct: 235 REGKDVTIVTFSKMVGFALKAAEKLAEEGISAEVINLRSIRPLDRATINASVRKTSRLVT 294
Query: 408 VEESWPTCGIGSEILARVMETQAFNYLDAPALRVTGADIPMPYAKPLEMEALPRVENVIK 587
VEE +P G+ +EI A V+E ++F+YLDAP R+ GAD+PMPYA LE ALP++E++++
Sbjct: 295 VEEGFPQHGVCAEICASVVE-ESFSYLDAPVERIAGADVPMPYAANLERLALPQIEDIVR 353
Query: 588 SVKKVLH 608
+ K+ +
Sbjct: 354 ASKRACY 360
>sp|Q09171|ODPB_SCHPO Pyruvate dehydrogenase E1 component beta subunit, mitochondrial
precursor (PDHE1-B)
Length = 366
Score = 209 bits (531), Expect = 9e-54
Identities = 105/190 (55%), Positives = 144/190 (75%), Gaps = 1/190 (0%)
Frame = +3
Query: 48 LKVLSPYSSEDARGLLKSAIRDPDPVIVLENEVLYGHPFDTPDEALSPDFLIPIGKAKIE 227
LKV+SPYS+EDARGLLK+AIRDP+PV+VLENE+LYG F EALS DF++P G AK+E
Sbjct: 177 LKVVSPYSAEDARGLLKAAIRDPNPVVVLENEILYGKTFPISKEALSEDFVLPFGLAKVE 236
Query: 228 KTGTSVTLVSFSKGVDLCLNAANEL-AKIGVDCEVINLRSLRPLDMETVIKSVVKTHHLV 404
+ G +T+V S V L AA++L A GV+ EVINLRS+RPLD+ T+ SV KT+ +V
Sbjct: 237 RPGKDITIVGESISVVTALEAADKLKADYGVEAEVINLRSIRPLDINTIAASVKKTNRIV 296
Query: 405 TVEESWPTCGIGSEILARVMETQAFNYLDAPALRVTGADIPMPYAKPLEMEALPRVENVI 584
TV++++ GIGSEI A++ME+ AF+YLDAP RV+ AD+PMPY+ P+E ++P + V+
Sbjct: 297 TVDQAYSQHGIGSEIAAQIMESDAFDYLDAPVERVSMADVPMPYSHPVEAASVPNADVVV 356
Query: 585 KSVKKVLHIQ 614
+ KK L+I+
Sbjct: 357 AAAKKCLYIK 366
>sp|P52904|ODPB_PEA Pyruvate dehydrogenase E1 component beta subunit, mitochondrial
precursor (PDHE1-B)
Length = 359
Score = 201 bits (512), Expect = 1e-51
Identities = 104/187 (55%), Positives = 132/187 (70%)
Frame = +3
Query: 48 LKVLSPYSSEDARGLLKSAIRDPDPVIVLENEVLYGHPFDTPDEALSPDFLIPIGKAKIE 227
LKVL P+S+EDARGLLK+AIRDPDPV+ LENE+LYG F E L F +PIGKAKIE
Sbjct: 165 LKVLVPHSAEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFWLPIGKAKIE 224
Query: 228 KTGTSVTLVSFSKGVDLCLNAANELAKIGVDCEVINLRSLRPLDMETVIKSVVKTHHLVT 407
+ G VT+ +FSK V L AA L K G+ EVINLRS+RPLD T+ SV KT+ LVT
Sbjct: 225 REGKDVTITAFSKMVGFALKAAEILEKEGISAEVINLRSIRPLDRPTINASVRKTNRLVT 284
Query: 408 VEESWPTCGIGSEILARVMETQAFNYLDAPALRVTGADIPMPYAKPLEMEALPRVENVIK 587
VEE +P G+G+EI V+E ++F YLDA R+ GAD+PMPYA LE +P VE++++
Sbjct: 285 VEEGFPQHGVGAEICTSVIE-ESFGYLDATVERIGGADVPMPYAGNLERLVVPHVEDIVR 343
Query: 588 SVKKVLH 608
+ K+ H
Sbjct: 344 AAKRACH 350
>sp|Q9R9N4|ODPB_RHIME Pyruvate dehydrogenase E1 component, beta subunit
Length = 460
Score = 201 bits (510), Expect = 2e-51
Identities = 103/187 (55%), Positives = 135/187 (72%)
Frame = +3
Query: 48 LKVLSPYSSEDARGLLKSAIRDPDPVIVLENEVLYGHPFDTPDEALSPDFLIPIGKAKIE 227
LKV+ PY++ DA+GLLK+AIRDP+PVI LENE+LYG F+ P DF++PIGKA+I
Sbjct: 277 LKVVMPYTAADAKGLLKAAIRDPNPVIFLENEILYGQSFEVPK---LDDFVLPIGKARIH 333
Query: 228 KTGTSVTLVSFSKGVDLCLNAANELAKIGVDCEVINLRSLRPLDMETVIKSVVKTHHLVT 407
+TG TLVSF G+ + AA EL G+D E+I+LR++RP+D+ TVI+SV KT LVT
Sbjct: 334 RTGKDATLVSFGIGMTYAIKAAAELEAQGIDVEIIDLRTIRPMDLPTVIESVKKTGRLVT 393
Query: 408 VEESWPTCGIGSEILARVMETQAFNYLDAPALRVTGADIPMPYAKPLEMEALPRVENVIK 587
VEE +P +G+EI RVM+ QAF+YLDAP L + G D+PMPYA LE ALP V V+
Sbjct: 394 VEEGYPQSSVGTEIATRVMQ-QAFDYLDAPILTIAGKDVPMPYAANLEKLALPNVAEVVD 452
Query: 588 SVKKVLH 608
+VK V +
Sbjct: 453 AVKAVCY 459
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86,960,494
Number of Sequences: 369166
Number of extensions: 1790892
Number of successful extensions: 4402
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4211
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4342
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7066925600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)