Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_M07
(836 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9FUN0|HMT1_MAIZE Homocysteine S-methyltransferase 1 (S-... 135 2e-31
sp|Q8LAX0|HMT3_ARATH Homocysteine S-methyltransferase 3 (S-... 132 1e-30
sp|Q9SDL7|HMT1_ARATH Homocysteine S-methyltransferase 1 (S-... 130 4e-30
sp|Q9FUM9|HMT2_MAIZE Homocysteine S-methyltransferase 2 (S-... 125 1e-28
sp|Q9FUM8|HMT3_MAIZE Homocysteine S-methyltransferase 3 (S-... 124 4e-28
sp|Q9FUM7|HMT4_MAIZE Homocysteine S-methyltransferase 4 (S-... 122 1e-27
sp|Q47690|MMUM_ECOLI Homocysteine S-methyltransferase (S-me... 119 1e-26
sp|Q9M1W4|HMT2_ARATH Homocysteine S-methyltransferase 2 (S-... 113 6e-25
sp|P56707|SMTA_ASTBI Selenocysteine methyltransferase (SECY... 110 5e-24
sp|Q12525|MHT1_YEAST Homocysteine S-methyltransferase 1 (S-... 80 5e-15
>sp|Q9FUN0|HMT1_MAIZE Homocysteine S-methyltransferase 1 (S-methylmethionine:homocysteine
methyltransferase 1) (SMM:Hcy S-methyltransferase 1)
(ZmHMT-1)
Length = 323
Score = 135 bits (339), Expect = 2e-31
Identities = 92/268 (34%), Positives = 151/268 (56%), Gaps = 6/268 (2%)
Frame = +2
Query: 14 ILDGGSGFEAIQLCKGKIDISNHICWSSRLLHDDQLIVREIYKRFLCAGAEVITSLSYQA 193
++DGG A QL DI++ + WS+ L +V+E++ ++L AGA+VI S SYQA
Sbjct: 16 VIDGGF---ATQLEALGADINDPL-WSAACLITRPHLVKEVHMQYLEAGADVIISSSYQA 71
Query: 194 CPITLSSSFNMSIEDAKQLMVDSVLLAN---DEILK--YQSSKVERTSIMSAASIGSYGA 358
I + MS+ +A+ L+ SV LAN DE K + SK + AASIGSYGA
Sbjct: 72 T-IPGFIARGMSVAEAEDLLRTSVKLANEARDEFWKSTLRKSKPIYNRALVAASIGSYGA 130
Query: 359 HLSDLSEYTGSYVDKVSKFDLKTYYEEQISTLLIGKPDILAFETMPAMLDVELIIDLMRE 538
+L+D SEY+GSY ++ LK ++ ++ L PD++AFE +P ++ + +++L+ E
Sbjct: 131 YLADGSEYSGSYGADITAEKLKDFHRRRLQVLASAGPDLIAFEAIPNQMEAQALVELLEE 190
Query: 539 YP-NTSAWISMTSLDGIHTAHGELLDDVIEYINQYSTKNVIGFGLNCVIPLMVVKDTVCK 715
+WI +S+DG + GE D ++ +N +++ V G+NC P ++ +C+
Sbjct: 191 EKVQIPSWICFSSVDGKNLCSGESFADCLKILN--ASEKVAVVGVNCT-PPQFIEGIICE 247
Query: 716 IRSRLNPDKIIVLYPNTGEVLNSLNRHW 799
R + K I +YPN+GEV + + W
Sbjct: 248 FRKQTK--KAIAVYPNSGEVWDGRAKRW 273
>sp|Q8LAX0|HMT3_ARATH Homocysteine S-methyltransferase 3 (S-methylmethionine:homocysteine
methyltransferase 3) (SMM:Hcy S-methyltransferase 3)
(AtHMT-3)
Length = 347
Score = 132 bits (332), Expect = 1e-30
Identities = 96/276 (34%), Positives = 147/276 (53%), Gaps = 14/276 (5%)
Frame = +2
Query: 14 ILDGGSGFEAIQLCKGKIDISNHICWSSRLLHDDQLIVREIYKRFLCAGAEVITSLSYQA 193
++DGG A +L + DI++ + WS++ L +V +++ +L +GA +I + SYQA
Sbjct: 25 VVDGGF---ATELQRHGADINDPL-WSAKCLITSPHLVTKVHLDYLESGANIIITASYQA 80
Query: 194 CPITLSSSFNMSIEDAKQLMVDSVLLAND--EILKYQ----------SSKVERTSIMSAA 337
I + +S+ +A+ L+ SV + + EI + + K R I+ AA
Sbjct: 81 T-IQGFVAKGLSVGEAENLLRRSVEITYEAREIFYNRCTKGSWDFAYAGKASRRPILVAA 139
Query: 338 SIGSYGAHLSDLSEYTGSYVDKVSKFDLKTYYEEQISTLLIGKPDILAFETMPAMLDVEL 517
S+GSYGA+L+D SEY+G Y D VSK LK ++ ++ L D++AFET+P L+ E
Sbjct: 140 SVGSYGAYLADGSEYSGIYGDSVSKETLKDFHRRRVQILAKSGADLIAFETIPNKLEAEA 199
Query: 518 IIDLMREYP-NTSAWISMTSLDGIHTAHGELLDDVIEYIN-QYSTKNVIGFGLNCVIPLM 691
DL+ E + AW S TS DG+ G D V+E S KNV+ G+NC P
Sbjct: 200 YADLLEEEDIDIPAWFSFTSKDGVSVPRG---DSVVECAKVADSCKNVVAIGINCTAPRY 256
Query: 692 VVKDTVCKIRSRLNPDKIIVLYPNTGEVLNSLNRHW 799
+ I R K IV+YPN+GEV + LN+ W
Sbjct: 257 I---HALIISLRQMTRKPIVVYPNSGEVYDGLNKKW 289
>sp|Q9SDL7|HMT1_ARATH Homocysteine S-methyltransferase 1 (S-methylmethionine:homocysteine
methyltransferase 1) (SMM:Hcy S-methyltransferase 1)
(AtHMT-1)
Length = 326
Score = 130 bits (327), Expect = 4e-30
Identities = 85/247 (34%), Positives = 142/247 (57%), Gaps = 6/247 (2%)
Frame = +2
Query: 77 NHICWSSRLLHDDQLIVREIYKRFLCAGAEVITSLSYQACPITLSSSFNMSIEDAKQLMV 256
N WS+ L + +++ ++ +L AGA+++ + SYQA I S +SIE+++ L+
Sbjct: 39 NDPLWSAVSLIKNPELIKRVHMEYLEAGADIVVTSSYQAT-IPGFLSRGLSIEESESLLQ 97
Query: 257 DSVLLANDEILKY--QSSKVERTSI---MSAASIGSYGAHLSDLSEYTGSYVDKVSKFDL 421
SV LA + ++ + SKV S + AASIGSYGA+L+D SEY+G Y + VS L
Sbjct: 98 KSVELAVEARDRFWEKVSKVSGHSYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKL 157
Query: 422 KTYYEEQISTLLIGKPDILAFETMPAMLDVELIIDLMREYP-NTSAWISMTSLDGIHTAH 598
K ++ ++ L+ PD+LAFET+P L+ + ++L+ E AWI TS+DG
Sbjct: 158 KDFHRRRLQVLVEAGPDLLAFETIPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPS 217
Query: 599 GELLDDVIEYINQYSTKNVIGFGLNCVIPLMVVKDTVCKIRSRLNPDKIIVLYPNTGEVL 778
GE ++ +E +N+ + N+ G+NC P + + + + ++L K IV+YPN+GEV
Sbjct: 218 GESFEECLEPLNK--SNNIYAVGINCAPPQFI--ENLIRKFAKLT-KKAIVVYPNSGEVW 272
Query: 779 NSLNRHW 799
+ + W
Sbjct: 273 DGKAKQW 279
>sp|Q9FUM9|HMT2_MAIZE Homocysteine S-methyltransferase 2 (S-methylmethionine:homocysteine
methyltransferase 2) (SMM:Hcy S-methyltransferase 2)
(ZmHMT-2)
Length = 339
Score = 125 bits (314), Expect = 1e-28
Identities = 84/268 (31%), Positives = 144/268 (53%), Gaps = 6/268 (2%)
Frame = +2
Query: 14 ILDGGSGFEAIQLCKGKIDISNHICWSSRLLHDDQLIVREIYKRFLCAGAEVITSLSYQA 193
+LDGG A +L D+++ + WS++ L ++R+++ +L AGA +I + SYQA
Sbjct: 25 VLDGGL---ATELEANGADLNDPL-WSAKCLLSSPHLIRKVHMDYLEAGANIIITASYQA 80
Query: 194 CPITLSSSFNMSIEDAKQLMVDSVLLANDEILKYQSSKVERTS-----IMSAASIGSYGA 358
I S S E ++ L+ SV +A + + +E+++ ++ AAS+GSYGA
Sbjct: 81 T-IQGFESKGFSKEQSENLLTKSVEIALEAREMFLKEHLEKSTPIQHPVLVAASLGSYGA 139
Query: 359 HLSDLSEYTGSYVDKVSKFDLKTYYEEQISTLLIGKPDILAFETMPAMLDVELIIDLMRE 538
+L+D SEY+G Y + +K LK ++ ++ L PD++AFET+P L+ E ++L+ E
Sbjct: 140 YLADGSEYSGDYGEAGTKEFLKDFHRRRLQVLAEAGPDLIAFETIPNKLEAEAYVELLEE 199
Query: 539 YP-NTSAWISMTSLDGIHTAHGELLDDVIEYINQYSTKNVIGFGLNCVIPLMVVKDTVCK 715
N AW S S DG+H G+ L + ++ + + G+NC P + +
Sbjct: 200 CNINIPAWFSFNSKDGVHIVSGDSLIECTTIADKCAKVGAV--GINCT-PPRFIHGLILS 256
Query: 716 IRSRLNPDKIIVLYPNTGEVLNSLNRHW 799
IR DK I++YPN+GE + + W
Sbjct: 257 IRK--VTDKPILIYPNSGERYDGEKKEW 282
>sp|Q9FUM8|HMT3_MAIZE Homocysteine S-methyltransferase 3 (S-methylmethionine:homocysteine
methyltransferase 3) (SMM:Hcy S-methyltransferase 3)
(ZmHMT-3)
Length = 338
Score = 124 bits (310), Expect = 4e-28
Identities = 82/268 (30%), Positives = 145/268 (54%), Gaps = 6/268 (2%)
Frame = +2
Query: 14 ILDGGSGFEAIQLCKGKIDISNHICWSSRLLHDDQLIVREIYKRFLCAGAEVITSLSYQA 193
+LDGG A +L D+++ + WS++ L ++R+++ +L AGA +I + SYQA
Sbjct: 25 VLDGGL---ATELEANGADLNDPL-WSAKCLLSSPHLIRKVHMDYLEAGANIIITASYQA 80
Query: 194 CPITLSSSFNMSIEDAKQLMVDSVLLANDEILKYQSSKVERTS-----IMSAASIGSYGA 358
I S S E ++ L+ SV +A + + +E+++ I+ AA++GSYGA
Sbjct: 81 T-IQGFESKGFSKEQSENLLTKSVQIALEAREMFLKEHLEKSTPIQHPILVAAALGSYGA 139
Query: 359 HLSDLSEYTGSYVDKVSKFDLKTYYEEQISTLLIGKPDILAFETMPAMLDVELIIDLMRE 538
+L+D SEY+G Y + +K LK ++ ++ L PD++AFET+P L+ + ++L+ E
Sbjct: 140 YLADGSEYSGDYGEAGTKEFLKDFHRRRLQVLAEAGPDLIAFETIPNKLEAQAYVELLEE 199
Query: 539 YP-NTSAWISMTSLDGIHTAHGELLDDVIEYINQYSTKNVIGFGLNCVIPLMVVKDTVCK 715
N +W+S S DG+H G+ L + ++ + + G+NC P + +
Sbjct: 200 CNINIPSWLSFNSKDGVHVVSGDSLIECATIADKCAKVGAV--GINCT-PPRFIHGLILS 256
Query: 716 IRSRLNPDKIIVLYPNTGEVLNSLNRHW 799
IR DK I++YPN+GE + + W
Sbjct: 257 IRK--VTDKPILIYPNSGERYDGEKKEW 282
>sp|Q9FUM7|HMT4_MAIZE Homocysteine S-methyltransferase 4 (S-methylmethionine:homocysteine
methyltransferase 4) (SMM:Hcy S-methyltransferase 4)
(ZmHMT-4)
Length = 342
Score = 122 bits (306), Expect = 1e-27
Identities = 87/270 (32%), Positives = 147/270 (54%), Gaps = 8/270 (2%)
Frame = +2
Query: 14 ILDGGSGFEAIQLCKGKIDISNHICWSSRLLHDDQLIVREIYKRFLCAGAEVITSLSYQA 193
++DGG G E L D+ + + WS++ L ++R+++ +L AGA+VI S SYQA
Sbjct: 26 VVDGGLGTE---LEAHGADLHDAL-WSAKCLASAPHLIRKVHLDYLEAGADVIISASYQA 81
Query: 194 CPITLSSSFNMSIEDAKQLMVDSVLLANDEILKY-----QSSKVERTSIMSAASIGSYGA 358
I S S +++++L+ SV +A + + +SS+ R + AAS+GSYGA
Sbjct: 82 T-IEGFQSRGFSRDESEELLRRSVHVAQEARRVFAAEGDRSSRRGRPPALVAASVGSYGA 140
Query: 359 HLSDLSEYTGSYVDKVSKFDLKTYYEEQISTLLIGKPDILAFETMPAMLDVELIIDLMRE 538
+ +D SEY+G Y ++K DLK ++ ++ L PD++AFET+P L+ ++ +L+ E
Sbjct: 141 YRADGSEYSGDYGKSMTKEDLKNFHRRRLQVLAGAGPDLIAFETIPNKLEAQVYAELLEE 200
Query: 539 YP-NTSAWISMTSLDGIHTAHGELLDDVIEYINQYSTKNVIGFGLNCVIPLMV--VKDTV 709
AW S TS DG++ A G+ +++ + S V G+NC P + + ++
Sbjct: 201 NGIRIPAWFSFTSKDGVNAASGDPINECAAVAD--SCPRVDAVGVNCTAPRFIHGLILSI 258
Query: 710 CKIRSRLNPDKIIVLYPNTGEVLNSLNRHW 799
K+ S K IV+YPN+GE + W
Sbjct: 259 KKVTS-----KPIVVYPNSGETYVAETNEW 283
>sp|Q47690|MMUM_ECOLI Homocysteine S-methyltransferase (S-methylmethionine:homocysteine
methyltransferase)
Length = 310
Score = 119 bits (298), Expect = 1e-26
Identities = 73/255 (28%), Positives = 131/255 (51%), Gaps = 13/255 (5%)
Frame = +2
Query: 89 WSSRLLHDDQLIVREIYKRFLCAGAEVITSLSYQACPITLSSSFNMSIEDAKQLMVDSVL 268
WS+++L ++ ++RE++ + AGA+ + SYQA P ++ + +K L+ SV
Sbjct: 39 WSAKVLVENPELIREVHLDYYRAGAQCAITASYQATPAGFAAR-GLDEAQSKALIGKSVE 97
Query: 269 LANDEILKYQSSKVERTSIMSAASIGSYGAHLSDLSEYTGSYVDKVSKFDLKTYYEEQIS 448
LA Y + + +++ A S+G YGA+L+D SEY G Y V F + ++ ++
Sbjct: 98 LARKAREAYLAENPQAGTLLVAGSVGPYGAYLADGSEYRGDYHCSVEAF--QAFHRPRVE 155
Query: 449 TLLIGKPDILAFETMPAMLDVELIIDLMREYPNTSAWISMTSLDGIHTAHGELLDDVIEY 628
LL D+LA ET+P ++E + +L+ YP AW S T D H + G L DV+
Sbjct: 156 ALLDAGADLLACETLPNFSEIEALAELLTAYPRARAWFSFTLRDSEHLSDGTPLRDVVAL 215
Query: 629 INQYSTKNVIGFGLNCVIPLMVVKDTVCKIRSRLNPDKI-IVLYPNTGEVLNSLNRHW-- 799
+ Y V+ G+NC+ +++T ++ + +V+YPN+GE +++++ W
Sbjct: 216 LAGY--PQVVALGINCI----ALENTTAALQHLHGLTVLPLVVYPNSGEHYDAVSKTWHH 269
Query: 800 ----------YFPEW 814
Y P+W
Sbjct: 270 HGEHCAQLADYLPQW 284
>sp|Q9M1W4|HMT2_ARATH Homocysteine S-methyltransferase 2 (S-methylmethionine:homocysteine
methyltransferase 2) (SMM:Hcy S-methyltransferase 2)
(AtHMT-2)
Length = 333
Score = 113 bits (283), Expect = 6e-25
Identities = 79/273 (28%), Positives = 144/273 (52%), Gaps = 11/273 (4%)
Frame = +2
Query: 14 ILDGGSGFEAIQLCKGKIDISNHICWSSRLLHDDQLIVREIYKRFLCAGAEVITSLSYQA 193
++DGG A + + D+++ + WS++ L ++ ++ +L AGA++I+S SYQA
Sbjct: 21 VIDGGL---ATEFERHGADLNDPL-WSAKCLVTSPHLIHTVHLDYLEAGADIISSASYQA 76
Query: 194 CPITLSSSFNMSIEDAKQLMVDSVLLANDEILKYQ-----SSKVE-----RTSIMSAASI 343
I + S E+++ L+ SV +A + Y SS ++ + I+ AAS+
Sbjct: 77 T-IQGFEAKGFSREESESLLKKSVEIATEARNSYYDKCGTSSSMDDKILKKRPILVAASV 135
Query: 344 GSYGAHLSDLSEYTGSYVDKVSKFDLKTYYEEQISTLLIGKPDILAFETMPAMLDVELII 523
GSYGA+L+D SEY+G Y D ++ LK ++ ++ L D++AFET+P ++ +
Sbjct: 136 GSYGAYLADGSEYSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFETIPNKIEAQAFA 195
Query: 524 DLMREYP-NTSAWISMTSLDGIHTAHGELLDDVIEYINQYSTKNVIGFGLNCVIPLMVVK 700
DL+ E W S S DG++ G+ + + I + + V+ G+NC P ++
Sbjct: 196 DLLEEGDVKIPGWFSFNSKDGVNVVSGDSIKECISIAE--NCEKVVAVGINCT-PPRFIE 252
Query: 701 DTVCKIRSRLNPDKIIVLYPNTGEVLNSLNRHW 799
V +I + K I++YPN+GE ++ + W
Sbjct: 253 GLVLEIEKVTS--KPILVYPNSGESYDADRKEW 283
>sp|P56707|SMTA_ASTBI Selenocysteine methyltransferase (SECYS-methyltransferase)
(SECYS-MT)
Length = 338
Score = 110 bits (275), Expect = 5e-24
Identities = 80/276 (28%), Positives = 140/276 (50%), Gaps = 14/276 (5%)
Frame = +2
Query: 14 ILDGGSGFEAIQLCKGKIDISNHICWSSRLLHDDQLIVREIYKRFLCAGAEVITSLSYQA 193
++ GG G E L + D+++ + WS++ L ++R+++ +L GA++I + SYQA
Sbjct: 18 VIAGGLGTE---LQRHGADLNDPL-WSAKCLLSCPHLIRQVHLDYLENGADIIITASYQA 73
Query: 194 CPITLSSSFNMSIEDAKQLMVDSVLLANDEILKYQSSKVERTS-------------IMSA 334
I + S E+ + L+ SV +A + Y E +S I+ A
Sbjct: 74 T-IQGFKAKGFSDEEGEALLRRSVEIAREARDLYYQRCAESSSDNGDDSRILKQRPILIA 132
Query: 335 ASIGSYGAHLSDLSEYTGSYVDKVSKFDLKTYYEEQISTLLIGKPDILAFETMPAMLDVE 514
S+GSYGA+L+D SE++G+Y D + LK ++ ++ L D+LAFE +P L+ +
Sbjct: 133 GSVGSYGAYLADGSEFSGNYGDAIKSETLKDFHRRKVQILADSGVDLLAFEAVPNKLEAQ 192
Query: 515 LIIDLMREYP-NTSAWISMTSLDGIHTAHGELLDDVIEYINQYSTKNVIGFGLNCVIPLM 691
DL+ E T AW + TS DG + G+ +++ S V+ G+NC P
Sbjct: 193 AYADLLEEENIITPAWFAFTSKDGNNVVSGDSIEECGSIAE--SCDKVVAVGINCT-PPR 249
Query: 692 VVKDTVCKIRSRLNPDKIIVLYPNTGEVLNSLNRHW 799
+ D + ++ K IV+YPN+GE +++ + W
Sbjct: 250 FIHDLILLLKK--VTAKPIVIYPNSGETYDAIRKEW 283
>sp|Q12525|MHT1_YEAST Homocysteine S-methyltransferase 1 (S-methylmethionine:homocysteine
methyltransferase 1) (SMM:Hcy S-methyltransferase 1)
Length = 324
Score = 80.5 bits (197), Expect = 5e-15
Identities = 66/280 (23%), Positives = 130/280 (46%), Gaps = 13/280 (4%)
Frame = +2
Query: 8 IKILDGGSGFEAIQLCKGKIDISNHICWSSRLLHDDQL---------IVREIYKRFLCAG 160
+ ILDGG G E L I+I++ + WS+ + +V E+Y+ F+ AG
Sbjct: 17 VLILDGGQGTE---LENRGININSPV-WSAAPFTSESFWEPSSQERKVVEEMYRDFMIAG 72
Query: 161 AEVITSLSYQACPITLSSSFNMSIEDAKQLMVDSVLLANDEILKYQSSKVERTSIMSAAS 340
A ++ +++YQA ++S + ++ A + +D ++ E + + + S
Sbjct: 73 ANILMTITYQANFQSISENTSIKTLAAYKRFLDKIVSFTREFIGEERYLI--------GS 124
Query: 341 IGSYGAHLSDLSEYTGSYVDKVSKFDLKTYYEEQISTLLIGKP-DILAFETMPAMLDVEL 517
IG + AH+S EYTG Y D +++ Q+ + D++ FET+P +++
Sbjct: 125 IGPWAAHVS--CEYTGDYGPHPENIDYYGFFKPQLENFNQNRDIDLIGFETIPNFHELKA 182
Query: 518 IIDLMREYPNTSAWISMTSLDGIHTAHGELLDDV---IEYINQYSTKNVIGFGLNCVIPL 688
I+ + + +I ++ D G L+++ I+ + KN++ G+NCV
Sbjct: 183 ILSWDEDIISKPFYIGLSVDDNSLLRDGTTLEEISVHIKGLGNKINKNLLLMGVNCV--S 240
Query: 689 MVVKDTVCKIRSRLNPDKIIVLYPNTGEVLNSLNRHWYFP 808
+ K+ P +++YPN+GE+ N + W+ P
Sbjct: 241 FNQSALILKMLHEHLPGMPLLVYPNSGEIYNPKEKTWHRP 280
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 96,979,325
Number of Sequences: 369166
Number of extensions: 1926995
Number of successful extensions: 5415
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5207
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5384
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8148988185
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)