Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_M02
(266 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P14625|ENPL_HUMAN Endoplasmin precursor (94 kDa glucose-... 40 0.001
sp|Q95M18|ENPL_BOVIN Endoplasmin precursor (94 kDa glucose-... 40 0.001
sp|P08712|ENPL_MESAU Endoplasmin (94 kDa glucose-regulated ... 40 0.002
sp|P41148|ENPL_CANFA Endoplasmin precursor (94 kDa glucose-... 40 0.002
sp|Q29092|ENPL_PIG Endoplasmin precursor (94 kDa glucose-re... 40 0.002
sp|P08110|ENPL_CHICK Endoplasmin precursor (Heat shock 108 ... 40 0.002
sp|O18750|ENPL_RABIT Endoplasmin (94 kDa glucose-regulated ... 39 0.005
sp|P08113|ENPL_MOUSE Endoplasmin precursor (Endoplasmic ret... 38 0.006
sp|P15108|HSC82_YEAST ATP-dependent molecular chaperone HSC... 38 0.008
sp|Q08277|HSP82_MAIZE Heat shock protein 82 37 0.014
>sp|P14625|ENPL_HUMAN Endoplasmin precursor (94 kDa glucose-regulated protein) (GRP94)
(gp96 homolog) (Tumor rejection antigen 1)
Length = 803
Score = 40.4 bits (93), Expect = 0.001
Identities = 24/56 (42%), Positives = 33/56 (58%), Gaps = 1/56 (1%)
Frame = +1
Query: 1 GYSLQASGGFADRIEKILRTSLNIPHDEAVEDD-EPIEEKPAEDEGEVPEPPKTEQ 165
GY L + + DRIE++LR SLNI D VE++ E E+ AED E E + E+
Sbjct: 726 GYLLPDTKAYGDRIERMLRLSLNIDPDAKVEEEPEEEPEETAEDTTEDTEQDEDEE 781
>sp|Q95M18|ENPL_BOVIN Endoplasmin precursor (94 kDa glucose-regulated protein) (GRP94)
Length = 804
Score = 40.4 bits (93), Expect = 0.001
Identities = 23/65 (35%), Positives = 35/65 (53%)
Frame = +1
Query: 1 GYSLQASGGFADRIEKILRTSLNIPHDEAVEDDEPIEEKPAEDEGEVPEPPKTEQPIENK 180
GY L + + DRIE++LR SLNI D VE++ EE+P E + E + ++ E
Sbjct: 726 GYLLPDTKAYGDRIERMLRLSLNIDPDAKVEEEP--EEEPEETTEDTAEDTEQDEEEEMD 783
Query: 181 EAVDK 195
D+
Sbjct: 784 AGTDE 788
>sp|P08712|ENPL_MESAU Endoplasmin (94 kDa glucose-regulated protein) (GRP94)
Length = 400
Score = 40.0 bits (92), Expect = 0.002
Identities = 21/61 (34%), Positives = 34/61 (55%)
Frame = +1
Query: 1 GYSLQASGGFADRIEKILRTSLNIPHDEAVEDDEPIEEKPAEDEGEVPEPPKTEQPIENK 180
GY L + +ADRIE++LR SLNI + VE++ E + ++ E E + + E +
Sbjct: 323 GYLLPDTKAYADRIERMLRLSLNIDPEAQVEEEPEEEPEDTTEDTEQDEEEEVDAGTEEE 382
Query: 181 E 183
E
Sbjct: 383 E 383
>sp|P41148|ENPL_CANFA Endoplasmin precursor (94 kDa glucose-regulated protein) (GRP94)
Length = 804
Score = 40.0 bits (92), Expect = 0.002
Identities = 22/64 (34%), Positives = 35/64 (54%)
Frame = +1
Query: 1 GYSLQASGGFADRIEKILRTSLNIPHDEAVEDDEPIEEKPAEDEGEVPEPPKTEQPIENK 180
GY L + + DRIE++LR SLNI + D +EE+P E+ E E + +++
Sbjct: 726 GYLLPDTKAYGDRIERMLRLSLNI------DPDAKVEEEPEEEPEETTEDTTEDTEQDDE 779
Query: 181 EAVD 192
E +D
Sbjct: 780 EEMD 783
>sp|Q29092|ENPL_PIG Endoplasmin precursor (94 kDa glucose-regulated protein) (GRP94)
(GP96 homolog) (98 kDa protein kinase) (PPK 98) (ppk98)
Length = 804
Score = 39.7 bits (91), Expect = 0.002
Identities = 23/65 (35%), Positives = 34/65 (52%)
Frame = +1
Query: 1 GYSLQASGGFADRIEKILRTSLNIPHDEAVEDDEPIEEKPAEDEGEVPEPPKTEQPIENK 180
GY L + + DRIE++LR SLNI D VE++ EE+P E + E + + E
Sbjct: 726 GYLLPDTKAYGDRIERMLRLSLNIDPDAKVEEEP--EEEPEETTEDTTEDTEQDDDEEMD 783
Query: 181 EAVDK 195
D+
Sbjct: 784 AGADE 788
>sp|P08110|ENPL_CHICK Endoplasmin precursor (Heat shock 108 kDa protein) (HSP108) (HSP
108) (Transferrin-binding protein)
Length = 795
Score = 39.7 bits (91), Expect = 0.002
Identities = 23/49 (46%), Positives = 30/49 (61%)
Frame = +1
Query: 1 GYSLQASGGFADRIEKILRTSLNIPHDEAVEDDEPIEEKPAEDEGEVPE 147
GY L + + DRIE++LR SLNI D VE +EP E + A +E E E
Sbjct: 725 GYMLPDTKEYGDRIERMLRLSLNIDLDAKVE-EEPEEPEDAAEEAEQDE 772
>sp|O18750|ENPL_RABIT Endoplasmin (94 kDa glucose-regulated protein) (GRP94)
Length = 716
Score = 38.5 bits (88), Expect = 0.005
Identities = 24/55 (43%), Positives = 33/55 (60%)
Frame = +1
Query: 1 GYSLQASGGFADRIEKILRTSLNIPHDEAVEDDEPIEEKPAEDEGEVPEPPKTEQ 165
GY L + + DRIE+I+R SLNI D VE++ EE+P ED E E + E+
Sbjct: 640 GYLLPDTKAYGDRIERIVRLSLNIDPDAKVEEEP--EEEP-EDTTEDTEQDEEEE 691
>sp|P08113|ENPL_MOUSE Endoplasmin precursor (Endoplasmic reticulum protein 99) (94 kDa
glucose-regulated protein) (GRP94) (ERP99) (Polymorphic
tumor rejection antigen 1) (Tumor rejection antigen
gp96)
Length = 802
Score = 38.1 bits (87), Expect = 0.006
Identities = 26/74 (35%), Positives = 38/74 (51%), Gaps = 9/74 (12%)
Frame = +1
Query: 1 GYSLQASGGFADRIEKILRTSLNIPHDEAVEDDEPIE--------EKPAEDEGEVPEPPK 156
GY L + + DRIE++LR SLNI + VE++ E E +DEGE +
Sbjct: 726 GYLLPDTKAYGDRIERMLRLSLNIDPEAQVEEEPEEEPEDTSEDAEDSEQDEGEEMDAGT 785
Query: 157 TEQPIE-NKEAVDK 195
E+ E KE+ +K
Sbjct: 786 EEEEEETEKESTEK 799
>sp|P15108|HSC82_YEAST ATP-dependent molecular chaperone HSC82 (Heat shock protein Hsp90
constitutive isoform) (82 kDa heat shock cognate
protein)
Length = 705
Score = 37.7 bits (86), Expect = 0.008
Identities = 20/52 (38%), Positives = 28/52 (53%), Gaps = 6/52 (11%)
Frame = +1
Query: 1 GYSLQASGGFADRIEKILRTSLNIPHDEAVE------DDEPIEEKPAEDEGE 138
G+SL+ FA RI +++ LNI DE E + P+EE PA+ E E
Sbjct: 651 GFSLEEPTSFASRINRLISLGLNIDEDEETETAPEASTEAPVEEVPADTEME 702
>sp|Q08277|HSP82_MAIZE Heat shock protein 82
Length = 715
Score = 37.0 bits (84), Expect = 0.014
Identities = 21/54 (38%), Positives = 28/54 (51%)
Frame = +1
Query: 1 GYSLQASGGFADRIEKILRTSLNIPHDEAVEDDEPIEEKPAEDEGEVPEPPKTE 162
G+SL FA RI ++L+ LNI D A ++D + PA DEG E E
Sbjct: 663 GFSLDDPNTFAARIHRMLKLGLNIDEDAAADED---ADMPALDEGAAEESKMEE 713
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.312 0.137 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,709,086
Number of Sequences: 369166
Number of extensions: 464114
Number of successful extensions: 2691
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2209
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2601
length of database: 68,354,980
effective HSP length: 58
effective length of database: 57,640,350
effective search space used: 1729210500
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)