Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_K10
(855 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Con... 67 5e-11
sp|O13035|SAP_CHICK Proactivator polypeptide precursor [Con... 65 2e-10
sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Con... 64 7e-10
sp|P10960|SAP_RAT Sulfated glycoprotein 1 precursor (SGP-1)... 53 1e-06
sp|Q61207|SAP_MOUSE Sulfated glycoprotein 1 precursor (SGP-... 50 1e-05
sp|P20097|SAP_CAVPO Saposin C (CO-beta-glucosidase) (Glucos... 49 1e-05
sp|P42211|ASPRX_ORYSA Aspartic proteinase precursor 47 5e-05
sp|P22355|PSPB_RAT Pulmonary surfactant-associated protein ... 45 3e-04
sp|P15285|PSPB_RABIT Pulmonary surfactant-associated protei... 44 4e-04
sp|P50405|PSPB_MOUSE Pulmonary surfactant-associated protei... 44 7e-04
>sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B (Sphingolipid activator protein 1) (SAP-1)
(Cerebroside sulfate activator) (CSAct) (Dispersin)
(Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 525
Score = 67.4 bits (163), Expect = 5e-11
Identities = 56/245 (22%), Positives = 102/245 (41%), Gaps = 39/245 (15%)
Frame = +1
Query: 67 VDRYGDILMTFIINHMDAKSICKLIHACTSVNVQ---------------------LKPIR 183
+++Y ++ + ++ HM K IC L C V ++PI+
Sbjct: 246 INQYSEVAIQMVM-HMQPKEICVLAGFCDEVKEMPMKTLVPAEVVSENVIPALGLVEPIK 304
Query: 184 K-----MKGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDE 348
K I C+ C VVK+ + I + TE EI + L + S QCQ++VD
Sbjct: 305 KDPAPAKADIYCEVCEFVVKEVAKLIDNNRTEEEILHALDKVCSKLPTSLAEQCQEVVDT 364
Query: 349 YFNLILNFILAEVDVDKICKFFGLCNA------------KLSGIECELCEHVMDEVIKVV 492
Y IL+ +L E + +C LC++ + G CE+C+ ++ + + +
Sbjct: 365 YGRSILSILLDEASPELVCSMLHLCSSRGLPAATVRVMPRKDGGFCEVCKKLVGYLDRNL 424
Query: 493 TSNHSEEVVSNFIKLKICPRL-SVIKNECVIIVDTYLPVIFSIARSTLNSKVLCTELKLC 669
N ++E + ++ K C L + +C V Y PV+ I ++ +C ++ C
Sbjct: 425 EKNSTKEQILAALE-KGCSFLPDQYRKQCDQFVTEYEPVLIEILVEVMDPSFVCLKIGAC 483
Query: 670 TLLSK 684
K
Sbjct: 484 PAAHK 488
Score = 56.2 bits (134), Expect = 1e-07
Identities = 29/125 (23%), Positives = 60/125 (48%), Gaps = 3/125 (2%)
Frame = +1
Query: 64 VVDRYGDILMTFIINHMDAKSICKLIHACTSVNVQLKPIRKMK---GIECDACRLVVKKF 234
VVD YG +++ +++ + +C ++H C+S + +R M G C+ C+ +V
Sbjct: 361 VVDTYGRSILSILLDEASPELVCSMLHLCSSRGLPAATVRVMPRKDGGFCEVCKKLVGYL 420
Query: 235 KEYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLILNFILAEVDVDKICKFF 414
+ +T+ +I L+ ++ + QC Q V EY +++ ++ +D +C
Sbjct: 421 DRNLEKNSTKEQILAALEKGCSFLPDQYRKQCDQFVTEYEPVLIEILVEVMDPSFVCLKI 480
Query: 415 GLCNA 429
G C A
Sbjct: 481 GACPA 485
Score = 53.9 bits (128), Expect = 6e-07
Identities = 32/105 (30%), Positives = 58/105 (55%), Gaps = 3/105 (2%)
Frame = +1
Query: 442 IECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRL-SVIKNECVIIVDTYLPVIFSI 618
I CE+CE V+ EV K++ +N +EE + + + K+C +L + + +C +VDTY I SI
Sbjct: 314 IYCEVCEFVVKEVAKLIDNNRTEEEILHALD-KVCSKLPTSLAEQCQEVVDTYGRSILSI 372
Query: 619 ARSTLNSKVLCTELKLCTL--LSKKKISFLMNRSGNETLKCEICR 747
+ +++C+ L LC+ L + + + G CE+C+
Sbjct: 373 LLDEASPELVCSMLHLCSSRGLPAATVRVMPRKDGG---FCEVCK 414
Score = 41.6 bits (96), Expect = 0.003
Identities = 28/102 (27%), Positives = 48/102 (47%), Gaps = 2/102 (1%)
Frame = +1
Query: 130 CKLIHACTSVNVQLKPIRKMKGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIF 309
C + C V KP +K + CD C+ V+ + D ATE EI +L+ ++
Sbjct: 42 CGAVQHCLQT-VWSKPT--VKSLPCDICKDVITAAGNLLKDNATEQEILMYLERTCDWLP 98
Query: 310 GSD-KIQCQQIVDEYFNLILNFILAEVD-VDKICKFFGLCNA 429
+ C++IVD Y +IL+ I ++ ++C LC +
Sbjct: 99 KPNMSASCKEIVDSYLPVILDMIKGQMSHPGEVCSALNLCES 140
Score = 37.7 bits (86), Expect = 0.041
Identities = 22/87 (25%), Positives = 45/87 (51%), Gaps = 3/87 (3%)
Frame = +1
Query: 433 LSGIECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRLSV--IKNECVIIVDTYLPV 606
+ + C++C+ V+ ++ N +E+ + +++ + C L + C IVD+YLPV
Sbjct: 58 VKSLPCDICKDVITAAGNLLKDNATEQEILMYLE-RTCDWLPKPNMSASCKEIVDSYLPV 116
Query: 607 IFSIARSTLNSK-VLCTELKLCTLLSK 684
I + + ++ +C+ L LC L K
Sbjct: 117 ILDMIKGQMSHPGEVCSALNLCESLQK 143
>sp|O13035|SAP_CHICK Proactivator polypeptide precursor [Contains: Saposin A; Saposin B;
Saposin C; Saposin D]
Length = 518
Score = 65.5 bits (158), Expect = 2e-10
Identities = 52/240 (21%), Positives = 104/240 (43%), Gaps = 34/240 (14%)
Frame = +1
Query: 67 VDRYGDILMTFIINHMDA--KSICKLIHACTSVN---------VQLKPIRKMKGIE---- 201
+ Y D+ + +++ D K IC ++ C SV Q+ KM+ +E
Sbjct: 244 ISEYSDLAIQMMMHMKDQQPKDICAMVGFCPSVKSVPLQTLVPAQVVHEVKMETVEKATV 303
Query: 202 -------CDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIF-GSDKIQCQQIVDEYFN 357
C+ C +VK+ + TE EI + ++ CY+ S K QC+ ++ Y
Sbjct: 304 QEKTFSVCEICETMVKEVTGLLESNKTEEEIVHEMEV-VCYLLPASVKDQCKDFIEVYGQ 362
Query: 358 LILNFILAEVDVDKICKFFGLCNA----------KLSGIECELCEHVMDEVIKVVTSNHS 507
+++ +L + + +C C A K +G C++C+ ++ K + N +
Sbjct: 363 ALIDMLLEATNPEAVCVMLKCCAANKPPQQPVVVKPAGGFCDICKMIVAYADKELEKNAT 422
Query: 508 EEVVSNFIKLKICPRL-SVIKNECVIIVDTYLPVIFSIARSTLNSKVLCTELKLCTLLSK 684
+ ++ K+C L + ++CV V+ Y PV+ + ++ +CT+L +C K
Sbjct: 423 TTEIEALLE-KVCHFLPESVSDQCVQFVEQYEPVVVQLLAEMMDPTFVCTKLGVCGAAKK 481
Score = 50.8 bits (120), Expect = 5e-06
Identities = 29/87 (33%), Positives = 52/87 (59%), Gaps = 3/87 (3%)
Frame = +1
Query: 433 LSGIECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRL--SVIKNECVIIVDTYLPV 606
++ I C+LC+ ++ V KV+ N +E+ + ++++ K C L + +EC IVD+YLPV
Sbjct: 59 VNSIPCDLCKELVTVVGKVLKDNGTEDEIRSYLE-KRCEFLPDQGLASECKEIVDSYLPV 117
Query: 607 IFSIARSTLNS-KVLCTELKLCTLLSK 684
I + + + +V+C+ L LC L K
Sbjct: 118 IMDMIKEEFDKPEVVCSALSLCQSLQK 144
Score = 49.7 bits (117), Expect = 1e-05
Identities = 47/231 (20%), Positives = 89/231 (38%), Gaps = 34/231 (14%)
Frame = +1
Query: 166 QLKPIRKMKGIE--CDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKIQ-CQQ 336
Q KP +K K E C C +V +E + AT + + C G C+
Sbjct: 183 QDKPKQKSKATEDVCQDCIRLVTDVQEAVRTNATFVKSLVAHAKEECDRLGPGMSDMCKS 242
Query: 337 IVDEYFNLILNFILAEVDVDK--ICKFFGLC------------------NAKLSGIE--- 447
+ EY +L + ++ D IC G C K+ +E
Sbjct: 243 YISEYSDLAIQMMMHMKDQQPKDICAMVGFCPSVKSVPLQTLVPAQVVHEVKMETVEKAT 302
Query: 448 --------CELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRLSVIKNECVIIVDTYLP 603
CE+CE ++ EV ++ SN +EE + + +++ + +K++C ++ Y
Sbjct: 303 VQEKTFSVCEICETMVKEVTGLLESNKTEEEIVHEMEVVCYLLPASVKDQCKDFIEVYGQ 362
Query: 604 VIFSIARSTLNSKVLCTELKLCTLLSKKKISFLMNRSGNETLKCEICRFAI 756
+ + N + +C LK C + ++ +G C+IC+ +
Sbjct: 363 ALIDMLLEATNPEAVCVMLKCCAANKPPQQPVVVKPAGG---FCDICKMIV 410
Score = 45.8 bits (107), Expect = 2e-04
Identities = 31/105 (29%), Positives = 51/105 (48%), Gaps = 3/105 (2%)
Frame = +1
Query: 124 SICKLIHACTSVNVQLKPIRKMKGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCY 303
S C + C NV KP + I CD C+ +V + + D TE EI+++L+ C
Sbjct: 41 SQCGAVKHCQQ-NVWSKPA--VNSIPCDLCKELVTVVGKVLKDNGTEDEIRSYLEKR-CE 96
Query: 304 IFGSDKI--QCQQIVDEYFNLILNFILAEVD-VDKICKFFGLCNA 429
+ +C++IVD Y +I++ I E D + +C LC +
Sbjct: 97 FLPDQGLASECKEIVDSYLPVIMDMIKEEFDKPEVVCSALSLCQS 141
>sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B-Val; Saposin B (Sphingolipid activator
protein 1) (SAP-1) (Cerebroside sulfate activator)
(CSAct) (Dispersin) (Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 524
Score = 63.5 bits (153), Expect = 7e-10
Identities = 52/240 (21%), Positives = 101/240 (42%), Gaps = 39/240 (16%)
Frame = +1
Query: 67 VDRYGDILMTFIINHMDAKSICKLIHACTSVNVQ---------------------LKPIR 183
+ +Y +I + ++ HM K IC L+ C V ++PI+
Sbjct: 245 ISQYSEIAIQMMM-HMQPKEICALVGFCDEVKEMPMQTLVPAKVASKNVIPALELVEPIK 303
Query: 184 KMK-----GIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDE 348
K + + C+ C +VK+ + I + TE EI + + S +CQ++VD
Sbjct: 304 KHEVPAKSDVYCEVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKSLSEECQEVVDT 363
Query: 349 YFNLILNFILAEVDVDKICKFFGLCNA------------KLSGIECELCEHVMDEVIKVV 492
Y + IL+ +L EV + +C LC+ G CE+C+ ++ + + +
Sbjct: 364 YGSSILSILLEEVSPELVCSMLHLCSGTRLPALTVHVTQPKDGGFCEVCKKLVGYLDRNL 423
Query: 493 TSNHSEEVVSNFIKLKICPRL-SVIKNECVIIVDTYLPVIFSIARSTLNSKVLCTELKLC 669
N +++ + ++ K C L + +C V Y PV+ I ++ +C ++ C
Sbjct: 424 EKNSTKQEILAALE-KGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGAC 482
Score = 52.4 bits (124), Expect = 2e-06
Identities = 32/107 (29%), Positives = 57/107 (53%), Gaps = 3/107 (2%)
Frame = +1
Query: 436 SGIECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRL-SVIKNECVIIVDTYLPVIF 612
S + CE+CE ++ EV K++ +N +E+ + + K+C +L + EC +VDTY I
Sbjct: 311 SDVYCEVCEFLVKEVTKLIDNNKTEKEILDAFD-KMCSKLPKSLSEECQEVVDTYGSSIL 369
Query: 613 SIARSTLNSKVLCTELKLC--TLLSKKKISFLMNRSGNETLKCEICR 747
SI ++ +++C+ L LC T L + + G CE+C+
Sbjct: 370 SILLEEVSPELVCSMLHLCSGTRLPALTVHVTQPKDGG---FCEVCK 413
Score = 52.4 bits (124), Expect = 2e-06
Identities = 26/123 (21%), Positives = 57/123 (46%), Gaps = 3/123 (2%)
Frame = +1
Query: 64 VVDRYGDILMTFIINHMDAKSICKLIHACTSVNVQLKPI---RKMKGIECDACRLVVKKF 234
VVD YG +++ ++ + + +C ++H C+ + + + G C+ C+ +V
Sbjct: 360 VVDTYGSSILSILLEEVSPELVCSMLHLCSGTRLPALTVHVTQPKDGGFCEVCKKLVGYL 419
Query: 235 KEYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLILNFILAEVDVDKICKFF 414
+ +T+ EI L+ ++ + QC Q V EY +++ ++ +D +C
Sbjct: 420 DRNLEKNSTKQEILAALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKI 479
Query: 415 GLC 423
G C
Sbjct: 480 GAC 482
Score = 43.1 bits (100), Expect = 0.001
Identities = 25/83 (30%), Positives = 43/83 (51%), Gaps = 2/83 (2%)
Frame = +1
Query: 187 MKGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSD-KIQCQQIVDEYFNLI 363
+K + CD C+ VV + + D ATE EI +L+ ++ + C++IVD Y +I
Sbjct: 58 VKSLPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNMSASCKEIVDSYLPVI 117
Query: 364 LNFILAEVD-VDKICKFFGLCNA 429
L+ I E+ ++C LC +
Sbjct: 118 LDIIKGEMSRPGEVCSALNLCES 140
Score = 40.4 bits (93), Expect = 0.006
Identities = 25/87 (28%), Positives = 45/87 (51%), Gaps = 3/87 (3%)
Frame = +1
Query: 433 LSGIECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRLSV--IKNECVIIVDTYLPV 606
+ + C++C+ V+ ++ N +EE + +++ K C L + C IVD+YLPV
Sbjct: 58 VKSLPCDICKDVVTAAGDMLKDNATEEEILVYLE-KTCDWLPKPNMSASCKEIVDSYLPV 116
Query: 607 IFSIARSTLNSK-VLCTELKLCTLLSK 684
I I + ++ +C+ L LC L K
Sbjct: 117 ILDIIKGEMSRPGEVCSALNLCESLQK 143
>sp|P10960|SAP_RAT Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)
Length = 554
Score = 52.8 bits (125), Expect = 1e-06
Identities = 37/131 (28%), Positives = 64/131 (48%), Gaps = 2/131 (1%)
Frame = +1
Query: 130 CKLIHACTSVNVQLKPIRKMKGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIF 309
C+ + C + V KP K + CD C+ VV + + D ATE EI ++L+ +I
Sbjct: 42 CRAVKHCQQM-VWSKPTAK--SLPCDICKTVVTEAGNLLKDNATEEEILHYLEKTCAWIH 98
Query: 310 GSD-KIQCQQIVDEYFNLILNFILAEV-DVDKICKFFGLCNAKLSGIECELCEHVMDEVI 483
S C+++VD Y +IL+ I E+ + ++C LC + L E++ ++
Sbjct: 99 DSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQS--------LQEYLAEQNQ 150
Query: 484 KVVTSNHSEEV 516
+ + SN EV
Sbjct: 151 RQLESNKIPEV 161
Score = 45.1 bits (105), Expect = 3e-04
Identities = 32/147 (21%), Positives = 61/147 (41%), Gaps = 26/147 (17%)
Frame = +1
Query: 67 VDRYGDILMTFIINHMDAKSICKLIHACTSVN--------------------------VQ 168
VD+Y ++ + ++ HM K IC ++ C V +
Sbjct: 244 VDQYSEVAVQMMM-HMQPKEICVMVGFCDEVKRVPMRTLVPATEAIKNILPALELTDPYE 302
Query: 169 LKPIRKMKGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDE 348
I+ I C C+LV++K E I + ATE + L + +CQ+++
Sbjct: 303 QDVIQAQNVIFCQVCQLVMRKLSELIINNATEELLIKGLSKACSLLPAPASTKCQEVLVT 362
Query: 349 YFNLILNFILAEVDVDKICKFFGLCNA 429
+ +L+ ++ EV+ + +C LC+A
Sbjct: 363 FGPSLLDVLMHEVNPNFLCGVISLCSA 389
Score = 43.9 bits (102), Expect = 6e-04
Identities = 24/84 (28%), Positives = 47/84 (55%), Gaps = 3/84 (3%)
Frame = +1
Query: 442 IECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRL--SVIKNECVIIVDTYLPVIFS 615
+ C++C+ V+ E ++ N +EE + ++++ K C + S + C +VD+YLPVI
Sbjct: 61 LPCDICKTVVTEAGNLLKDNATEEEILHYLE-KTCAWIHDSSLSASCKEVVDSYLPVILD 119
Query: 616 IARSTL-NSKVLCTELKLCTLLSK 684
+ + + N +C+ L LC L +
Sbjct: 120 MIKGEMSNPGEVCSALNLCQSLQE 143
Score = 37.0 bits (84), Expect = 0.070
Identities = 18/74 (24%), Positives = 36/74 (48%)
Frame = +1
Query: 202 CDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLILNFILA 381
C+ C+ +V + + +T+ EI L+ ++ + QC + V EY L+L ++
Sbjct: 439 CEVCKKLVIYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDEFVAEYEPLLLEILVE 498
Query: 382 EVDVDKICKFFGLC 423
+D +C G+C
Sbjct: 499 VMDPSFVCSKIGVC 512
>sp|Q61207|SAP_MOUSE Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)
Length = 557
Score = 49.7 bits (117), Expect = 1e-05
Identities = 32/111 (28%), Positives = 56/111 (50%), Gaps = 2/111 (1%)
Frame = +1
Query: 190 KGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSD-KIQCQQIVDEYFNLIL 366
K + CD C+ VV + + D AT+ EI ++L+ +I S C+++VD Y +IL
Sbjct: 59 KSLPCDICKTVVTEAGNLLKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVIL 118
Query: 367 NFILAEV-DVDKICKFFGLCNAKLSGIECELCEHVMDEVIKVVTSNHSEEV 516
+ I E+ + ++C LC + L E++ ++ K + SN EV
Sbjct: 119 DMIKGEMSNPGEVCSALNLCQS--------LQEYLAEQNQKQLESNKIPEV 161
Score = 49.3 bits (116), Expect = 1e-05
Identities = 37/155 (23%), Positives = 64/155 (41%), Gaps = 28/155 (18%)
Frame = +1
Query: 67 VDRYGDILMTFIINHMDA--KSICKLIHACTSVN-VQLKP-------------------- 177
VD+Y ++ + +++ D K IC L C V V +K
Sbjct: 244 VDQYSEVCVQMLMHMQDQQPKEICVLAGFCNEVKRVPMKTLVPATETIKNILPALEMMDP 303
Query: 178 -----IRKMKGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIV 342
++ I C C+ V+ KF E I + ATE + L N + + +CQ++V
Sbjct: 304 YEQNLVQAHNVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEVV 363
Query: 343 DEYFNLILNFILAEVDVDKICKFFGLCNAKLSGIE 447
+ +L+ + EV+ +C GLC A+ +E
Sbjct: 364 GTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVE 398
Score = 42.7 bits (99), Expect = 0.001
Identities = 23/84 (27%), Positives = 47/84 (55%), Gaps = 3/84 (3%)
Frame = +1
Query: 442 IECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRL--SVIKNECVIIVDTYLPVIFS 615
+ C++C+ V+ E ++ N ++E + ++++ K C + S + C +VD+YLPVI
Sbjct: 61 LPCDICKTVVTEAGNLLKDNATQEEILHYLE-KTCEWIHDSSLSASCKEVVDSYLPVILD 119
Query: 616 IARSTL-NSKVLCTELKLCTLLSK 684
+ + + N +C+ L LC L +
Sbjct: 120 MIKGEMSNPGEVCSALNLCQSLQE 143
Score = 37.4 bits (85), Expect = 0.054
Identities = 33/155 (21%), Positives = 60/155 (38%), Gaps = 35/155 (22%)
Frame = +1
Query: 64 VVDRYGDILMTFIINHMDAKSICKLIHACTS---------------VNVQLK-------- 174
VV +G L+ I+ ++ S+C +I C + V+ LK
Sbjct: 362 VVGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQP 421
Query: 175 ------------PIRKMKGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSD 318
P +K G C+ C+ +V + + +T+ EI L+ ++
Sbjct: 422 AQPKQSALPAHVPPQKNGGF-CEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPY 480
Query: 319 KIQCQQIVDEYFNLILNFILAEVDVDKICKFFGLC 423
+ QC V EY L+L ++ +D +C G+C
Sbjct: 481 QKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVC 515
>sp|P20097|SAP_CAVPO Saposin C (CO-beta-glucosidase) (Glucosylceramidase activator)
(Sphingolipid activator protein 2) (SAP-2)
Length = 81
Score = 49.3 bits (116), Expect = 1e-05
Identities = 26/78 (33%), Positives = 41/78 (52%)
Frame = +1
Query: 190 KGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLILN 369
+ + C AC VVKK E I + TE +I + L + + S CQ++VD Y + I+
Sbjct: 1 ESVTCKACEYVVKKVMELIDNNRTEEKIIHALDSVCALLPESVSEVCQEVVDTYGDSIVA 60
Query: 370 FILAEVDVDKICKFFGLC 423
+L E+ + +C GLC
Sbjct: 61 LLLQEMSPELVCSELGLC 78
Score = 45.1 bits (105), Expect = 3e-04
Identities = 22/77 (28%), Positives = 47/77 (61%), Gaps = 1/77 (1%)
Frame = +1
Query: 442 IECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRLSVIKNE-CVIIVDTYLPVIFSI 618
+ C+ CE+V+ +V++++ +N +EE + + + +C L +E C +VDTY I ++
Sbjct: 3 VTCKACEYVVKKVMELIDNNRTEEKIIHALD-SVCALLPESVSEVCQEVVDTYGDSIVAL 61
Query: 619 ARSTLNSKVLCTELKLC 669
++ +++C+EL LC
Sbjct: 62 LLQEMSPELVCSELGLC 78
>sp|P42211|ASPRX_ORYSA Aspartic proteinase precursor
Length = 496
Score = 47.4 bits (111), Expect = 5e-05
Identities = 27/104 (25%), Positives = 50/104 (48%), Gaps = 18/104 (17%)
Frame = +1
Query: 325 QCQQIVDEYFNLILNFILAEVDVDKICKFFGLC-----NAKLSGIE-------------C 450
+C+++V EY +ILN ++A+ D K+C GLC + +GIE C
Sbjct: 311 ECKEVVSEYGEMILNLLIAQTDPQKVCSQVGLCMFDGKRSVSNGIESVVDKENLGSDAMC 370
Query: 451 ELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRLSVIKNECVI 582
+CE + + + N ++E++ N+ ++C RL E +
Sbjct: 371 SVCEMAVVWIENQLRENKTKELILNYAN-QLCERLPSPNGESTV 413
Score = 33.5 bits (75), Expect = 0.77
Identities = 17/92 (18%), Positives = 42/92 (45%), Gaps = 5/92 (5%)
Frame = +1
Query: 559 VIKNECVIIVDTYLPVIFSIARSTLNSKVLCTELKLCTLLSKKKI-----SFLMNRSGNE 723
+I EC +V Y +I ++ + + + +C+++ LC K+ + S + +
Sbjct: 307 IISTECKEVVSEYGEMILNLLIAQTDPQKVCSQVGLCMFDGKRSVSNGIESVVDKENLGS 366
Query: 724 TLKCEICRFAILEVRTLLTDPGFQKIFENFFN 819
C +C A++ + L + +++ N+ N
Sbjct: 367 DAMCSVCEMAVVWIENQLRENKTKELILNYAN 398
Score = 31.2 bits (69), Expect = 3.8
Identities = 22/105 (20%), Positives = 43/105 (40%), Gaps = 13/105 (12%)
Frame = +1
Query: 64 VVDRYGDILMTFIINHMDAKSICKLIHAC-----TSVNVQLKPIRKMKGI----ECDACR 216
VV YG++++ +I D + +C + C SV+ ++ + + + C C
Sbjct: 315 VVSEYGEMILNLLIAQTDPQKVCSQVGLCMFDGKRSVSNGIESVVDKENLGSDAMCSVCE 374
Query: 217 LVVKKFKEYISDPATETEIKNFLKNDFCYIF----GSDKIQCQQI 339
+ V + + + T+ I N+ N C G + C QI
Sbjct: 375 MAVVWIENQLRENKTKELILNY-ANQLCERLPSPNGESTVSCHQI 418
>sp|P22355|PSPB_RAT Pulmonary surfactant-associated protein B precursor (SP-B)
(Pulmonary surfactant-associated proteolipid SPL(Phe))
Length = 376
Score = 44.7 bits (104), Expect = 3e-04
Identities = 28/144 (19%), Positives = 64/144 (44%), Gaps = 9/144 (6%)
Frame = +1
Query: 64 VVDRYGDILMTFIINHMDAKSICKLIHACTSVNV------QLKPIRK---MKGIECDACR 216
+ +RY +L+ ++ + + +C L+ C++ + L+P+ + ++ EC C+
Sbjct: 239 LAERYTVLLLDALLGRVVPQLVCGLVLRCSTADAIGPALPALEPLIEKWPLQDTECHFCK 298
Query: 217 LVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLILNFILAEVDVD 396
V+ + T + + C F D+ +C+Q V+++ +L + D
Sbjct: 299 SVINQAWN------TSEQAMPQAMHQACLRFWLDRQKCEQFVEQHMPQLLALVPRSQDAH 352
Query: 397 KICKFFGLCNAKLSGIECELCEHV 468
C+ G+C A S ++C H+
Sbjct: 353 TSCQALGVCEAPASPLQCFQTPHL 376
Score = 40.0 bits (92), Expect = 0.008
Identities = 22/98 (22%), Positives = 48/98 (48%), Gaps = 7/98 (7%)
Frame = +1
Query: 403 CKFFGLCNAKLSGIE-----CELCEHVMDEVIKVVTSNHSEEVVSNFIKLK--ICPRLSV 561
C+ G C ++ G C+ CE ++ + K+ + ++ + F++ + I P L +
Sbjct: 47 CRALGHCLQEVWGHAGANDLCQECEDIVHLLTKMTKEDAFQDTIRKFLEQECDILP-LKL 105
Query: 562 IKNECVIIVDTYLPVIFSIARSTLNSKVLCTELKLCTL 675
+ C ++D YLP++ + + K +C+ + LC L
Sbjct: 106 LVPRCRQVLDVYLPLVIDYFQGQIKPKAICSHVGLCPL 143
Score = 38.1 bits (87), Expect = 0.031
Identities = 19/76 (25%), Positives = 37/76 (48%), Gaps = 2/76 (2%)
Frame = +1
Query: 202 CDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKI--QCQQIVDEYFNLILNFI 375
C C +V + + A + I+ FL+ + C I + +C+Q++D Y L++++
Sbjct: 67 CQECEDIVHLLTKMTKEDAFQDTIRKFLEQE-CDILPLKLLVPRCRQVLDVYLPLVIDYF 125
Query: 376 LAEVDVDKICKFFGLC 423
++ IC GLC
Sbjct: 126 QGQIKPKAICSHVGLC 141
>sp|P15285|PSPB_RABIT Pulmonary surfactant-associated protein B precursor (SP-B) (6 kDa
protein) (Pulmonary surfactant-associated proteolipid
SPL(Phe))
Length = 370
Score = 44.3 bits (103), Expect = 4e-04
Identities = 35/143 (24%), Positives = 64/143 (44%), Gaps = 9/143 (6%)
Frame = +1
Query: 64 VVDRYGDILMTFIINHMDAKSICKLIHACTSVNV--QLKPIRKM-------KGIECDACR 216
+ +RY IL+ ++ H+ + +C L+ C+SV+ Q+ P + + EC C
Sbjct: 233 LAERYTVILLEVLLGHVLPQLVCGLVLRCSSVDSIGQVPPTLEALPGEWLPQDPECRLCM 292
Query: 217 LVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLILNFILAEVDVD 396
V + + IS+ + + C DK +C+Q V+ + +L+ + D
Sbjct: 293 SVTTQARN-ISEQTRPQAVYHA-----CLSSQLDKQECEQFVELHTPQLLSLLSRGWDAR 346
Query: 397 KICKFFGLCNAKLSGIECELCEH 465
IC+ G C A LS ++C H
Sbjct: 347 AICQALGACVATLSPLQCIQSPH 369
Score = 40.0 bits (92), Expect = 0.008
Identities = 22/96 (22%), Positives = 49/96 (51%), Gaps = 7/96 (7%)
Frame = +1
Query: 403 CKFFGLCNAKLSGIE-----CELCEHVMDEVIKVVTSNHSEEVVSNFIKLK--ICPRLSV 561
CK G C ++ G C+ C+ +++ + K+ ++ + F++ + + P L +
Sbjct: 50 CKALGHCLQEVWGHVGADDLCQECQDIVNILTKMTKEAIFQDTIRKFLEHECDVLP-LKL 108
Query: 562 IKNECVIIVDTYLPVIFSIARSTLNSKVLCTELKLC 669
+ +C ++D Y P+ + +S +N+K +C L LC
Sbjct: 109 LVPQCHHVLDVYFPLTITYFQSQINAKAICQHLGLC 144
Score = 38.9 bits (89), Expect = 0.018
Identities = 18/76 (23%), Positives = 38/76 (50%), Gaps = 2/76 (2%)
Frame = +1
Query: 202 CDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKI--QCQQIVDEYFNLILNFI 375
C C+ +V + + + I+ FL+++ C + + QC ++D YF L + +
Sbjct: 70 CQECQDIVNILTKMTKEAIFQDTIRKFLEHE-CDVLPLKLLVPQCHHVLDVYFPLTITYF 128
Query: 376 LAEVDVDKICKFFGLC 423
++++ IC+ GLC
Sbjct: 129 QSQINAKAICQHLGLC 144
>sp|P50405|PSPB_MOUSE Pulmonary surfactant-associated protein B precursor (SP-B)
(Pulmonary surfactant-associated proteolipid SPL(Phe))
Length = 377
Score = 43.5 bits (101), Expect = 7e-04
Identities = 24/96 (25%), Positives = 48/96 (50%), Gaps = 7/96 (7%)
Frame = +1
Query: 403 CKFFGLCNAKLSGIE-----CELCEHVMDEVIKVVTSNHSEEVVSNFIKLK--ICPRLSV 561
C+ G C ++ G C+ CE ++ + K+ + +E + F++ + I P L +
Sbjct: 48 CRALGHCLQEVWGHAGANDLCQECEDIVHLLTKMTKEDAFQEAIRKFLEQECDILP-LKL 106
Query: 562 IKNECVIIVDTYLPVIFSIARSTLNSKVLCTELKLC 669
+ C ++D YLP++ +S +N K +C + LC
Sbjct: 107 LVPRCRQVLDVYLPLVIDYFQSQINPKAICNHVGLC 142
Score = 42.7 bits (99), Expect = 0.001
Identities = 27/144 (18%), Positives = 64/144 (44%), Gaps = 9/144 (6%)
Frame = +1
Query: 64 VVDRYGDILMTFIINHMDAKSICKLIHACTSVNVQ------LKPIRK---MKGIECDACR 216
+ +RY +L+ ++ + + +C L+ C++ + ++P+ + ++ EC C+
Sbjct: 240 LAERYTVLLLDALLGRVVPQLVCGLVLRCSTEDAMGPALPAVEPLIEEWPLQDTECHFCK 299
Query: 217 LVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLILNFILAEVDVD 396
V+ + T + + C F D+ +C+Q V+++ +L + D
Sbjct: 300 SVINQAWN------TSEQAMPQAMHQACLRFWLDRQKCEQFVEQHMPQLLALVPRSQDAH 353
Query: 397 KICKFFGLCNAKLSGIECELCEHV 468
C+ G+C A S ++C H+
Sbjct: 354 ITCQALGVCEAPASPLQCFQTPHL 377
Score = 39.3 bits (90), Expect = 0.014
Identities = 19/76 (25%), Positives = 39/76 (51%), Gaps = 2/76 (2%)
Frame = +1
Query: 202 CDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKI--QCQQIVDEYFNLILNFI 375
C C +V + + A + I+ FL+ + C I + +C+Q++D Y L++++
Sbjct: 68 CQECEDIVHLLTKMTKEDAFQEAIRKFLEQE-CDILPLKLLVPRCRQVLDVYLPLVIDYF 126
Query: 376 LAEVDVDKICKFFGLC 423
++++ IC GLC
Sbjct: 127 QSQINPKAICNHVGLC 142
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 88,904,538
Number of Sequences: 369166
Number of extensions: 1744450
Number of successful extensions: 5068
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4799
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5051
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8438301375
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)