Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_J03
(545 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q90944|PGLB_CHICK Dermatan sulfate proteoglycan 3 precur... 34 0.27
sp|Q00155|VG34_ICHV1 Hypothetical gene 34 protein 31 1.7
sp|P97464|EXT1_MOUSE Exostosin-1 (Glucuronosyl-N-acetylgluc... 31 2.2
sp|Q16394|EXT1_HUMAN Exostosin-1 (Glucuronosyl-N-acetylgluc... 31 2.2
sp|Q8K4Q0|RPTOR_MOUSE Regulatory associated protein of mTOR... 30 3.8
sp|Q58365|HIS8_METJA Histidinol-phosphate aminotransferase ... 30 5.0
sp|P28976|RRPL_TSWV1 RNA-directed RNA polymerase (L protein) 29 6.5
sp|P70186|PGLB_MOUSE Dermatan sulfate proteoglycan 3 precur... 29 6.5
sp|P30619|SEC1_YEAST Protein transport protein SEC1 29 6.5
sp|Q02224|CENPE_HUMAN Centromeric protein E (CENP-E protein) 29 8.5
>sp|Q90944|PGLB_CHICK Dermatan sulfate proteoglycan 3 precursor (Epiphycan) (Small
chondroitin/dermatan sulfate proteoglycan)
(Proteoglycan-Lb) (PG-Lb)
Length = 316
Score = 33.9 bits (76), Expect = 0.27
Identities = 19/58 (32%), Positives = 30/58 (51%), Gaps = 1/58 (1%)
Frame = +1
Query: 49 IFSSSLKHIKIPTPEEFIKLFCQIPTTQPVFNDTFNKIEHHSQLFR-VHDIRVTADPL 219
I ++L H+ +P PE L Q Q + DTF K+ S + R + DIR+ +P+
Sbjct: 238 ITDNNLDHVPLPLPESLQALHLQNNNIQEMHEDTFCKMRDFSYVRRALEDIRLDGNPI 295
>sp|Q00155|VG34_ICHV1 Hypothetical gene 34 protein
Length = 441
Score = 31.2 bits (69), Expect = 1.7
Identities = 31/95 (32%), Positives = 48/95 (50%), Gaps = 5/95 (5%)
Frame = +1
Query: 61 SLKHIKIPTPEEF--IKLFCQIPTTQPVF-NDTFN--KIEHHSQLFRVHDIRVTADPLYN 225
S+K TP F I L I TT +F + T N K+ H + ++D+ T P+Y+
Sbjct: 192 SMKQKNFLTPFFFSSINLAGPIETTNQLFISMTINTQKLTHET----IYDLGKTLYPIYS 247
Query: 226 SLENDSKFLLSDSIEVPFVKCDHYIQRIPKIGSIY 330
LE D+KF ++ F++C +I KIG I+
Sbjct: 248 LLEVDTKFNWFCNMIALFLEC--FINTPNKIGMIW 280
>sp|P97464|EXT1_MOUSE Exostosin-1 (Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-
acetylglucosaminyl-proteoglycan
4-alpha-N-acetylglucosaminyltransferase) (Multiple
exostoses protein 1 homolog)
Length = 746
Score = 30.8 bits (68), Expect = 2.2
Identities = 17/53 (32%), Positives = 29/53 (54%), Gaps = 1/53 (1%)
Frame = +1
Query: 289 DHYIQRIPKIGSIYSDCLQFDSFQSNLIKIPK-KRKAITEVCIGKKCKLENCF 444
DH+ R P + Q ++ S++ P+ KR A + + GKKC++E+CF
Sbjct: 52 DHFWPRFPDALRPFFPWDQLENEDSSVHISPRQKRDANSSIYKGKKCRMESCF 104
>sp|Q16394|EXT1_HUMAN Exostosin-1 (Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-
acetylglucosaminyl-proteoglycan
4-alpha-N-acetylglucosaminyltransferase) (Putative tumor
suppressor protein EXT1) (Multiple exostoses protein 1)
Length = 746
Score = 30.8 bits (68), Expect = 2.2
Identities = 17/53 (32%), Positives = 29/53 (54%), Gaps = 1/53 (1%)
Frame = +1
Query: 289 DHYIQRIPKIGSIYSDCLQFDSFQSNLIKIPK-KRKAITEVCIGKKCKLENCF 444
DH+ R P + Q ++ S++ P+ KR A + + GKKC++E+CF
Sbjct: 52 DHFWPRFPDALRPFVPWDQLENEDSSVHISPRQKRDANSSIYKGKKCRMESCF 104
>sp|Q8K4Q0|RPTOR_MOUSE Regulatory associated protein of mTOR (Raptor) (P150 target of
rapamycin (TOR)-scaffold protein)
Length = 1335
Score = 30.0 bits (66), Expect = 3.8
Identities = 22/74 (29%), Positives = 34/74 (45%), Gaps = 4/74 (5%)
Frame = +1
Query: 295 YIQRIPK----IGSIYSDCLQFDSFQSNLIKIPKKRKAITEVCIGKKCKLENCFYSLFFT 462
Y+Q+ P+ S+ D FD + + + K +T + I + L C FT
Sbjct: 1218 YLQKHPEGHIVSVSVNGDVRFFDPRMPESVNVMQIVKGLTALDIHPQANLIACGSMNQFT 1277
Query: 463 NLYNTNKELLINIK 504
+YN N EL+ NIK
Sbjct: 1278 AIYNGNGELINNIK 1291
>sp|Q58365|HIS8_METJA Histidinol-phosphate aminotransferase (Imidazole acetol-phosphate
transaminase)
Length = 373
Score = 29.6 bits (65), Expect = 5.0
Identities = 19/70 (27%), Positives = 33/70 (47%), Gaps = 6/70 (8%)
Frame = +1
Query: 187 VHDIRVTADPLYNSLEN--DSKFLLSDS----IEVPFVKCDHYIQRIPKIGSIYSDCLQF 348
V D + + LYN L+ D K S++ +E+ +K + + + K G I DC F
Sbjct: 284 VRDGIKSREMLYNGLKKFKDIKVYPSEANYLLVELKTMKAKEFCEELLKRGVIVRDCTSF 343
Query: 349 DSFQSNLIKI 378
D N +++
Sbjct: 344 DGLGDNYVRV 353
>sp|P28976|RRPL_TSWV1 RNA-directed RNA polymerase (L protein)
Length = 2875
Score = 29.3 bits (64), Expect = 6.5
Identities = 24/130 (18%), Positives = 51/130 (39%)
Frame = +1
Query: 142 NDTFNKIEHHSQLFRVHDIRVTADPLYNSLENDSKFLLSDSIEVPFVKCDHYIQRIPKIG 321
N K+ S+ R+ ++++ Y ++ +D ++ + + R+P++G
Sbjct: 2321 NKMIVKVNATSRQIRLENVKLVVKIKYENVNSDVWDIIESQKSL--------VLRLPEVG 2372
Query: 322 SIYSDCLQFDSFQSNLIKIPKKRKAITEVCIGKKCKLENCFYSLFFTNLYNTNKELLINI 501
+SD + ++ IK K R + I L + ++ T E L+NI
Sbjct: 2373 EFFSDMYKTADSETETIKTIKNRLMTSLTFIEAFGNLSQQIKEIVDDDIRETMDEFLMNI 2432
Query: 502 KCEFSEDFNN 531
+ E N
Sbjct: 2433 RDTCLEGLEN 2442
>sp|P70186|PGLB_MOUSE Dermatan sulfate proteoglycan 3 precursor (Epiphycan) (Small
chondroitin/dermatan sulfate proteoglycan)
(Proteoglycan-Lb) (PG-Lb)
Length = 322
Score = 29.3 bits (64), Expect = 6.5
Identities = 17/58 (29%), Positives = 30/58 (51%), Gaps = 1/58 (1%)
Frame = +1
Query: 49 IFSSSLKHIKIPTPEEFIKLFCQIPTTQPVFNDTFNKIEHHSQLFR-VHDIRVTADPL 219
I +SL HI +P PE L Q + DTF +++ + + + + DIR+ +P+
Sbjct: 244 ITDNSLDHIPLPLPESLRALHLQNNDILEMHEDTFCNVKNLTYVRKALEDIRLDGNPI 301
>sp|P30619|SEC1_YEAST Protein transport protein SEC1
Length = 724
Score = 29.3 bits (64), Expect = 6.5
Identities = 33/115 (28%), Positives = 54/115 (46%), Gaps = 8/115 (6%)
Frame = +2
Query: 86 LQKNLSNYFVKFQQRNQFSMI------LSIKSSTTVNYLEYMIYESLLIHCIIL*KTIRN 247
+++NLS + + F +I L++K T ++ L Y+I +L II I+
Sbjct: 393 IEQNLSGFGMDFSGEKIKHIIDDLLPALAMKEPTILDKLRYIIAYALFRGGIIELDFIK- 451
Query: 248 FC*VIVSKCHLLNAIIIFNEYRKSVQFIPIV--YNLIVFNLI**KSRKKEKLLQK 406
LLN I + +E+ Q++ I Y+LI F LI K + K+K QK
Sbjct: 452 ----------LLNFIGVTHEHENFQQYLKIFRNYDLIDFKLI--KDKPKDKPFQK 494
>sp|Q02224|CENPE_HUMAN Centromeric protein E (CENP-E protein)
Length = 2663
Score = 28.9 bits (63), Expect = 8.5
Identities = 18/60 (30%), Positives = 31/60 (51%), Gaps = 3/60 (5%)
Frame = +1
Query: 373 KIPKKRKAITEVCIGKKCKLENCFYSLFF---TNLYNTNKELLINIKCEFSEDFNNKSEI 543
++ KRK C+GK K++N Y+ F TN+ +L IN+ E E ++S++
Sbjct: 412 ELKAKRKRRVTWCLGKINKMKNSNYADQFNIPTNITTKTHKLSINLLREIDESVCSESDV 471
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 56,326,533
Number of Sequences: 369166
Number of extensions: 1071520
Number of successful extensions: 2894
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2820
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2894
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 3783975580
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)