Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_H23
(925 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P14882|PCCA_RAT Propionyl-CoA carboxylase alpha chain, m... 269 9e-72
sp|P05165|PCCA_HUMAN Propionyl-CoA carboxylase alpha chain,... 266 6e-71
sp|Q91ZA3|PCCA_MOUSE Propionyl-CoA carboxylase alpha chain,... 266 8e-71
sp|Q58626|PYCA_METJA Pyruvate carboxylase subunit A (Pyruvi... 147 3e-35
sp|P49787|ACCC_BACSU Biotin carboxylase (A subunit of acety... 134 5e-31
sp|Q96RQ3|MCCA_HUMAN Methylcrotonoyl-CoA carboxylase alpha ... 133 6e-31
sp|Q42523|MCCA_ARATH Methylcrotonoyl-CoA carboxylase alpha ... 129 2e-29
sp|O30019|PYCA_ARCFU Pyruvate carboxylase subunit A (Pyruvi... 129 2e-29
sp|P24182|ACCC_ECOLI Biotin carboxylase (A subunit of acety... 126 1e-28
sp|Q99MR8|MCCA_MOUSE Methylcrotonoyl-CoA carboxylase alpha ... 126 1e-28
>sp|P14882|PCCA_RAT Propionyl-CoA carboxylase alpha chain, mitochondrial precursor
(PCCase alpha subunit) (Propanoyl-CoA:carbon dioxide
ligase alpha subunit)
Length = 704
Score = 269 bits (687), Expect = 9e-72
Identities = 139/277 (50%), Positives = 191/277 (68%)
Frame = +1
Query: 4 SARGQVEHPISELITGVDIVHQMIRVGKGHPLLLKQSDISVNGWAVECRVYAEDPYKSFG 183
+ R QVEHP++E ITG+D+V +MI V KG+PL KQ DI ++GWAVECRVYAEDPYKSFG
Sbjct: 327 NTRLQVEHPVTECITGLDLVQEMILVAKGYPLRHKQEDIPISGWAVECRVYAEDPYKSFG 386
Query: 184 LPSVGRLTSYKEPLHIPNVRCDSGIQEGSEISIYYDPMICKLVTYGENRDYALATMVKAL 363
LPS+GRL+ Y+EP+H+P VR DSGIQ GS+ISIY+DPMI KLVTYG +R AL M AL
Sbjct: 387 LPSIGRLSQYQEPIHLPGVRVDSGIQPGSDISIYHDPMISKLVTYGSDRAEALKRMEDAL 446
Query: 364 DSYVIRGVTHNIPLLRDIVTEPRFVSGNITTNYLPEVYPDGFKGKQLSPKETGLLEAMSA 543
DSYVIRGVTHNIPLLR+++ RFV G+I+T +L +VYPDGFKG L+P E L A+++
Sbjct: 447 DSYVIRGVTHNIPLLREVIINTRFVKGDISTKFLSDVYPDGFKGHMLTPSERDQLLAIAS 506
Query: 544 VVFVKNEIRNKTFLKRDRTSIGQNPESFRGDLSHPEEWNLSISHGLDSKDKTQNLHVTVS 723
+FV +++R + F + R + R D++ +W LS+ L +D T V S
Sbjct: 507 SLFVASQLRAQRFQEHSRVPV------IRPDVA---KWELSVK--LHDEDHT----VVAS 551
Query: 724 DDRSVFHVAFENGHSFEIKNNFHLADKIIVQNIDNEE 834
++ F+V + G + + ++LA ++ N+D +
Sbjct: 552 NNGPTFNVEVD-GSKLNVTSTWNLASPLLSVNVDGTQ 587
>sp|P05165|PCCA_HUMAN Propionyl-CoA carboxylase alpha chain, mitochondrial precursor
(PCCase alpha subunit) (Propanoyl-CoA:carbon dioxide
ligase alpha subunit)
Length = 703
Score = 266 bits (680), Expect = 6e-71
Identities = 139/277 (50%), Positives = 190/277 (68%)
Frame = +1
Query: 4 SARGQVEHPISELITGVDIVHQMIRVGKGHPLLLKQSDISVNGWAVECRVYAEDPYKSFG 183
+ R QVEHP++E ITG+D+V +MIRV KG+PL KQ+DI +NGWAVECRVYAEDPYKSFG
Sbjct: 326 NTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFG 385
Query: 184 LPSVGRLTSYKEPLHIPNVRCDSGIQEGSEISIYYDPMICKLVTYGENRDYALATMVKAL 363
LPS+GRL+ Y+EPLH+P VR DSGIQ GS+ISIYYDPMI KL+TYG +R AL M AL
Sbjct: 386 LPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADAL 445
Query: 364 DSYVIRGVTHNIPLLRDIVTEPRFVSGNITTNYLPEVYPDGFKGKQLSPKETGLLEAMSA 543
D+YVIRGVTHNI LLR+++ RFV G+I+T +L +VYPDGFKG L+ E L A+++
Sbjct: 446 DNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIAS 505
Query: 544 VVFVKNEIRNKTFLKRDRTSIGQNPESFRGDLSHPEEWNLSISHGLDSKDKTQNLHVTVS 723
+FV ++R + F + R + + D+++ W LS+ L K T V S
Sbjct: 506 SLFVAFQLRAQHFQENSRMPV------IKPDIAN---WELSVK--LHDKVHT----VVAS 550
Query: 724 DDRSVFHVAFENGHSFEIKNNFHLADKIIVQNIDNEE 834
++ SVF V + G + + ++LA ++ ++D +
Sbjct: 551 NNGSVFSVEVD-GSKLNVTSTWNLASPLLSVSVDGTQ 586
>sp|Q91ZA3|PCCA_MOUSE Propionyl-CoA carboxylase alpha chain, mitochondrial precursor
(PCCase alpha subunit) (Propanoyl-CoA:carbon dioxide
ligase alpha subunit)
Length = 724
Score = 266 bits (679), Expect = 8e-71
Identities = 139/277 (50%), Positives = 189/277 (68%)
Frame = +1
Query: 4 SARGQVEHPISELITGVDIVHQMIRVGKGHPLLLKQSDISVNGWAVECRVYAEDPYKSFG 183
+ R QVEHP++E ITG+D+V +MI V KG+PL KQ DI ++GWAVECRVYAEDPYKSFG
Sbjct: 347 NTRLQVEHPVTECITGLDLVQEMILVAKGYPLRHKQEDIPISGWAVECRVYAEDPYKSFG 406
Query: 184 LPSVGRLTSYKEPLHIPNVRCDSGIQEGSEISIYYDPMICKLVTYGENRDYALATMVKAL 363
LPS+GRL+ Y+EP+H+P VR DSGIQ GS+ISIYYDPMI KLVTYG +R AL M AL
Sbjct: 407 LPSIGRLSQYQEPIHLPGVRVDSGIQPGSDISIYYDPMISKLVTYGSDRAEALKRMEDAL 466
Query: 364 DSYVIRGVTHNIPLLRDIVTEPRFVSGNITTNYLPEVYPDGFKGKQLSPKETGLLEAMSA 543
D+YVIRGVTHNIPLLR+++ RFV G+I+T +L +VYPDGFKG L+ E L A+++
Sbjct: 467 DNYVIRGVTHNIPLLREVIINTRFVKGDISTKFLSDVYPDGFKGHTLTLSERNQLLAIAS 526
Query: 544 VVFVKNEIRNKTFLKRDRTSIGQNPESFRGDLSHPEEWNLSISHGLDSKDKTQNLHVTVS 723
VFV +++R + F + R + R D++ +W LS+ L +D T V S
Sbjct: 527 SVFVASQLRAQRFQEHSRVPV------IRPDVA---KWELSVK--LHDEDHT----VVAS 571
Query: 724 DDRSVFHVAFENGHSFEIKNNFHLADKIIVQNIDNEE 834
++ F V + G + + ++LA ++ N+D +
Sbjct: 572 NNGPAFTVEVD-GSKLNVTSTWNLASPLLSVNVDGTQ 607
>sp|Q58626|PYCA_METJA Pyruvate carboxylase subunit A (Pyruvic carboxylase A)
Length = 501
Score = 147 bits (372), Expect = 3e-35
Identities = 80/188 (42%), Positives = 117/188 (62%), Gaps = 1/188 (0%)
Frame = +1
Query: 4 SARGQVEHPISELITGVDIVHQMIRVGKGHPLLLKQSDISVNGWAVECRVYAEDPYKSFG 183
+ R QVEH ++E +TG+D+V MI++ G L LKQ D+ + G A+ECR+ AEDP F
Sbjct: 289 NTRIQVEHTVTEQVTGIDLVKAMIKIAAGEELTLKQEDVKIRGHAIECRINAEDPLNDF- 347
Query: 184 LPSVGRLTSYKEPLHIPNVRCDSGIQEGSEISIYYDPMICKLVTYGENRDYALATMVKAL 363
+P G++ Y+ P P VR DSG+ G+EI YYD MI KL+TYG +R+ A+A M +AL
Sbjct: 348 VPCPGKIKLYRSPGG-PGVRIDSGVYGGAEIPPYYDSMIAKLITYGNSREEAIARMKRAL 406
Query: 364 DSYVIRGVTHNIPLLRDIVTEPRFVSGNITTNYLPE-VYPDGFKGKQLSPKETGLLEAMS 540
YVI GV NIP R ++ E F+ GNI+T+Y+ + ++ K + + + L +S
Sbjct: 407 REYVIIGVKTNIPFHRAVLEEENFLKGNISTHYVEQNMHKLREKMVKYALESRDLYSVVS 466
Query: 541 AVVFVKNE 564
VF KN+
Sbjct: 467 EKVFEKNK 474
>sp|P49787|ACCC_BACSU Biotin carboxylase (A subunit of acetyl-CoA carboxylase) (ACC)
Length = 450
Score = 134 bits (336), Expect = 5e-31
Identities = 67/154 (43%), Positives = 94/154 (61%)
Frame = +1
Query: 4 SARGQVEHPISELITGVDIVHQMIRVGKGHPLLLKQSDISVNGWAVECRVYAEDPYKSFG 183
+ R QVEHP++E++TG D++ + I+V G L LKQ D+ GWA+ECR+ AE+P K+F
Sbjct: 291 NTRIQVEHPVTEMVTGTDLIKEQIKVASGMELSLKQEDVEFEGWAIECRINAENPSKNF- 349
Query: 184 LPSVGRLTSYKEPLHIPNVRCDSGIQEGSEISIYYDPMICKLVTYGENRDYALATMVKAL 363
+PS G + Y P + VR DS G I YYD MI K++TYG+ RD A+A M +AL
Sbjct: 350 MPSPGEIKMYLPPGGL-GVRVDSAAYPGYSIPPYYDSMIAKVITYGKTRDEAIARMKRAL 408
Query: 364 DSYVIRGVTHNIPLLRDIVTEPRFVSGNITTNYL 465
+VI G+ IP ++ FVSG T +L
Sbjct: 409 SEFVIEGIETTIPFHLKLLEHETFVSGEFNTKFL 442
>sp|Q96RQ3|MCCA_HUMAN Methylcrotonoyl-CoA carboxylase alpha chain, mitochondrial precursor
(3-Methylcrotonyl-CoA carboxylase 1) (MCCase alpha
subunit) (3-methylcrotonyl-CoA:carbon dioxide ligase
alpha subunit) (3-Methylcrotonyl-CoA carboxylase
biotin-containing subunit)
Length = 725
Score = 133 bits (335), Expect = 6e-31
Identities = 79/263 (30%), Positives = 136/263 (51%)
Frame = +1
Query: 4 SARGQVEHPISELITGVDIVHQMIRVGKGHPLLLKQSDISVNGWAVECRVYAEDPYKSFG 183
+ R QVEHP++E+ITG D+V +R+ G + L Q +I++ G A E R+YAEDP +F
Sbjct: 337 NTRLQVEHPVTEMITGTDLVEWQLRIAAGEKIPLSQEEITLQGHAFEARIYAEDPSNNF- 395
Query: 184 LPSVGRLTSYKEPLHIPNVRCDSGIQEGSEISIYYDPMICKLVTYGENRDYALATMVKAL 363
+P G L P P+ R ++G+++G E+S++YDPMI KLV + +R AL + +L
Sbjct: 396 MPVAGPLVHLSTPRADPSTRIETGVRQGDEVSVHYDPMIAKLVVWAADRQAALTKLRYSL 455
Query: 364 DSYVIRGVTHNIPLLRDIVTEPRFVSGNITTNYLPEVYPDGFKGKQLSPKETGLLEAMSA 543
Y I G+ NI L ++ P F +GN+ T+++P+ + ++ + KE+ A+
Sbjct: 456 RQYNIVGLPTNIDFLLNLSGHPEFEAGNVHTDFIPQHHKQLLLSRKAAAKESLCQAALGL 515
Query: 544 VVFVKNEIRNKTFLKRDRTSIGQNPESFRGDLSHPEEWNLSISHGLDSKDKTQNLHVTVS 723
++ K T D+ S P S S N+S + + KD N+ + V+
Sbjct: 516 ILKEKAMTDTFTLQAHDQFS----PFS----SSSGRRLNISYTRNMTLKDGKNNVAIAVT 567
Query: 724 DDRSVFHVAFENGHSFEIKNNFH 792
+ + +F++ N +
Sbjct: 568 YNHDGSYSMQIEDKTFQVLGNLY 590
>sp|Q42523|MCCA_ARATH Methylcrotonoyl-CoA carboxylase alpha chain, mitochondrial
precursor (3-Methylcrotonyl-CoA carboxylase 1) (MCCase
alpha subunit) (3-methylcrotonyl-CoA:carbon dioxide
ligase alpha subunit)
Length = 734
Score = 129 bits (323), Expect = 2e-29
Identities = 65/162 (40%), Positives = 99/162 (61%)
Frame = +1
Query: 4 SARGQVEHPISELITGVDIVHQMIRVGKGHPLLLKQSDISVNGWAVECRVYAEDPYKSFG 183
+ R QVEHP++E+I G D+V IRV G PL L QS++ ++G A E R+YAE+ K F
Sbjct: 327 NTRLQVEHPVTEMIVGQDLVEWQIRVANGEPLPLSQSEVPMSGHAFEARIYAENVPKGF- 385
Query: 184 LPSVGRLTSYKEPLHIPNVRCDSGIQEGSEISIYYDPMICKLVTYGENRDYALATMVKAL 363
LP+ G L Y+ P+VR ++G+++G +S++YDPMI KLV +G NR AL + L
Sbjct: 386 LPATGVLNHYRPVAVSPSVRVETGVEQGDTVSMHYDPMIAKLVVWGGNRGEALVKLKDCL 445
Query: 364 DSYVIRGVTHNIPLLRDIVTEPRFVSGNITTNYLPEVYPDGF 489
++ + GV NI L+ + + F GN+ T+++ D F
Sbjct: 446 SNFQVAGVPTNINFLQKLASHKEFAVGNVETHFIEHHKSDLF 487
>sp|O30019|PYCA_ARCFU Pyruvate carboxylase subunit A (Pyruvic carboxylase A)
Length = 506
Score = 129 bits (323), Expect = 2e-29
Identities = 69/156 (44%), Positives = 95/156 (60%)
Frame = +1
Query: 4 SARGQVEHPISELITGVDIVHQMIRVGKGHPLLLKQSDISVNGWAVECRVYAEDPYKSFG 183
++R QVEH I+E++TG+DIV IR+ G L Q D+++ G A+ECR+ AEDP +
Sbjct: 288 NSRLQVEHTITEVVTGIDIVKYQIRIAYGEELRHGQEDVAIRGHAIECRINAEDPVNFY- 346
Query: 184 LPSVGRLTSYKEPLHIPNVRCDSGIQEGSEISIYYDPMICKLVTYGENRDYALATMVKAL 363
P GR+ Y+ P I +R DSGI G I YD MI KL+ YGE R+ A+A M +AL
Sbjct: 347 -PRSGRILHYRSPGGI-GIRVDSGIHMGYRIPEEYDSMISKLIAYGETREEAIARMKRAL 404
Query: 364 DSYVIRGVTHNIPLLRDIVTEPRFVSGNITTNYLPE 471
Y+I GV NIP ++ + FV GNI T ++ E
Sbjct: 405 YEYIIEGVETNIPFHFAVLNDEEFVRGNIHTKFVEE 440
>sp|P24182|ACCC_ECOLI Biotin carboxylase (A subunit of acetyl-CoA carboxylase) (ACC)
Length = 449
Score = 126 bits (316), Expect = 1e-28
Identities = 67/154 (43%), Positives = 94/154 (61%)
Frame = +1
Query: 4 SARGQVEHPISELITGVDIVHQMIRVGKGHPLLLKQSDISVNGWAVECRVYAEDPYKSFG 183
+ R QVEHP++E+ITGVD++ + +R+ G PL +KQ ++ V G AVECR+ AEDP +
Sbjct: 290 NTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHAVECRINAEDP--NTF 347
Query: 184 LPSVGRLTSYKEPLHIPNVRCDSGIQEGSEISIYYDPMICKLVTYGENRDYALATMVKAL 363
LPS G++T + P VR +S I G + YYD MI KL+ YGENRD A+A M AL
Sbjct: 348 LPSPGKITRFHAPGGF-GVRWESHIYAGYTVPPYYDSMIGKLICYGENRDVAIARMKNAL 406
Query: 364 DSYVIRGVTHNIPLLRDIVTEPRFVSGNITTNYL 465
+I G+ N+ L I+ + F G +YL
Sbjct: 407 QELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYL 440
>sp|Q99MR8|MCCA_MOUSE Methylcrotonoyl-CoA carboxylase alpha chain, mitochondrial precursor
(3-Methylcrotonyl-CoA carboxylase 1) (MCCase alpha
subunit) (3-methylcrotonyl-CoA:carbon dioxide ligase
alpha subunit) (3-Methylcrotonyl-CoA carboxylase
biotin-containing subunit)
Length = 717
Score = 126 bits (316), Expect = 1e-28
Identities = 77/266 (28%), Positives = 132/266 (49%)
Frame = +1
Query: 4 SARGQVEHPISELITGVDIVHQMIRVGKGHPLLLKQSDISVNGWAVECRVYAEDPYKSFG 183
+ R QVEHP++E+ITG D+V +R+ G + L Q +I + G A E R+YAEDP +F
Sbjct: 333 NTRLQVEHPVTEMITGTDLVEWQLRIAAGEKIPLSQEEIPLQGHAFEARIYAEDPDNNF- 391
Query: 184 LPSVGRLTSYKEPLHIPNVRCDSGIQEGSEISIYYDPMICKLVTYGENRDYALATMVKAL 363
+P G L P + R ++G+++G E+S++YDPMI KLV + +R AL+ + L
Sbjct: 392 MPGAGPLVHLSTPSADMSTRIETGVRQGDEVSVHYDPMIAKLVVWASDRQSALSKLRYCL 451
Query: 364 DSYVIRGVTHNIPLLRDIVTEPRFVSGNITTNYLPEVYPDGFKGKQLSPKETGLLEAMSA 543
Y I G+ N+ L + P F +GN+ T+++P+ + D KE+ A+
Sbjct: 452 HQYNIVGLRSNVDFLLRLSGHPEFEAGNVHTDFIPQHHKDLLPSHSTIAKESVCQAALGL 511
Query: 544 VVFVKNEIRNKTFLKRDRTSIGQNPESFRGDLSHPEEWNLSISHGLDSKDKTQNLHVTVS 723
++ K +D+ S P SF S N+S + + + ++ + V+
Sbjct: 512 ILKEKEMTSAFKLHTQDQFS----PFSF----SSGRRLNISYTRNMTLRSGKSDIVIAVT 563
Query: 724 DDRSVFHVAFENGHSFEIKNNFHLAD 801
+R + + SF + + D
Sbjct: 564 YNRDGSYDMQIDNKSFRVLGDLSSED 589
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 103,626,421
Number of Sequences: 369166
Number of extensions: 2094503
Number of successful extensions: 4907
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4703
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4861
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9462723610
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)