Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_H04-2
(302 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O55236|MCE1_MOUSE mRNA capping enzyme (HCE) (MCE1) [Incl... 75 4e-14
sp|O60942|MCE1_HUMAN mRNA capping enzyme (HCE) (HCAP1) [Inc... 74 1e-13
sp|Q17607|MCE1_CAEEL mRNA capping enzyme [Includes: Polynuc... 57 1e-08
sp|P40997|MCE1_SCHPO mRNA capping enzyme alpha subunit (mRN... 40 0.002
sp|Q7SB53|MCE1_NEUCR mRNA capping enzyme alpha subunit (mRN... 36 0.024
sp|Q6BT58|MCE1_DEBHA mRNA capping enzyme alpha subunit (mRN... 32 0.60
sp|Q01159|MCE1_YEAST mRNA capping enzyme alpha subunit (mRN... 30 2.3
sp|P78587|MCE1_CANAL mRNA capping enzyme alpha subunit (mRN... 29 3.9
sp|Q59940|IDH_STRMU Isocitrate dehydrogenase [NADP] (Oxalos... 28 5.1
sp|Q9Z1G4|VPP1_MOUSE Vacuolar proton translocating ATPase 1... 28 8.7
>sp|O55236|MCE1_MOUSE mRNA capping enzyme (HCE) (MCE1) [Includes: Polynucleotide
5'-triphosphatase (mRNA 5'-triphosphatase) (TPase); mRNA
guanylyltransferase (GTP--RNA guanylyltransferase)
(GTase)]
Length = 597
Score = 75.5 bits (184), Expect = 4e-14
Identities = 41/94 (43%), Positives = 55/94 (58%), Gaps = 2/94 (2%)
Frame = +3
Query: 21 HYTNTLLDGEMVIDTYRGQKFPRFLIYDIMNINGDYVGDKLFTLRHKLIKTEIIDTRTSF 200
H +NTLLDGEM+ID GQ PR+LIYDI+ N VGD F +R + I+ EII R
Sbjct: 336 HLSNTLLDGEMIIDKVNGQAVPRYLIYDIIKFNAQPVGDCDFNIRLQCIEREIISPRHEK 395
Query: 201 VTPEM--RKNESFGIRIKDFWPIEKTRDIWEGKF 296
+ + + E F +R K F+ I +R + EG F
Sbjct: 396 MKTGLIDKTQEPFSVRPKQFFDINISRKLLEGNF 429
>sp|O60942|MCE1_HUMAN mRNA capping enzyme (HCE) (HCAP1) [Includes: Polynucleotide
5'-triphosphatase (mRNA 5'-triphosphatase) (TPase); mRNA
guanylyltransferase (GTP--RNA guanylyltransferase)
(GTase)]
Length = 597
Score = 73.9 bits (180), Expect = 1e-13
Identities = 41/94 (43%), Positives = 55/94 (58%), Gaps = 2/94 (2%)
Frame = +3
Query: 21 HYTNTLLDGEMVIDTYRGQKFPRFLIYDIMNINGDYVGDKLFTLRHKLIKTEIIDTRTSF 200
H +NTLLDGEM+ID GQ PR+LIYDI+ N VGD F +R + I+ EII R
Sbjct: 336 HLSNTLLDGEMIIDRVNGQAVPRYLIYDIIKFNSQPVGDCDFNVRLQCIEREIISPRHEK 395
Query: 201 VTPEM--RKNESFGIRIKDFWPIEKTRDIWEGKF 296
+ + + E F +R K F+ I +R + EG F
Sbjct: 396 MKTGLIDKTQEPFSVRNKPFFDICTSRKLLEGNF 429
>sp|Q17607|MCE1_CAEEL mRNA capping enzyme [Includes: Polynucleotide 5'-triphosphatase
(mRNA 5'-triphosphatase) (TPase); mRNA
guanylyltransferase (GTP--RNA guanylyltransferase)
(GTase)]
Length = 623
Score = 57.4 bits (137), Expect = 1e-08
Identities = 38/94 (40%), Positives = 54/94 (57%), Gaps = 6/94 (6%)
Frame = +3
Query: 33 TLLDGEMVIDTYRGQKF----PRFLIYDIMNINGDYVGDKLFTLRHKLIKTEIIDTRT-S 197
TL+D E++ID PR LIYDIM N V + F R ++IKTEIID RT +
Sbjct: 354 TLVDTEVIIDKVEINGAMCDQPRMLIYDIMRFNSVNVMKEPFYKRFEIIKTEIIDMRTAA 413
Query: 198 FVTPEMR-KNESFGIRIKDFWPIEKTRDIWEGKF 296
F T ++ +N+ +R KDF+ +E T ++ KF
Sbjct: 414 FKTGRLKHENQIMSVRRKDFYDLEATAKLFGPKF 447
>sp|P40997|MCE1_SCHPO mRNA capping enzyme alpha subunit (mRNA guanylyltransferase)
(GTP--RNA guanylyltransferase) (GTase)
Length = 402
Score = 39.7 bits (91), Expect = 0.002
Identities = 23/77 (29%), Positives = 40/77 (51%), Gaps = 1/77 (1%)
Frame = +3
Query: 6 KSPEEHYTNTLLDGEMVIDTY-RGQKFPRFLIYDIMNINGDYVGDKLFTLRHKLIKTEII 182
KS ++++ +TLLDGE+V+D Y G+K R+L++D + +G +L R + I
Sbjct: 112 KSGKKYHVDTLLDGELVLDIYPGGKKQLRYLVFDCLACDGIVYMSRLLDKRLGIFAKSIQ 171
Query: 183 DTRTSFVTPEMRKNESF 233
+ MR+ F
Sbjct: 172 KPLDEYTKTHMRETAIF 188
>sp|Q7SB53|MCE1_NEUCR mRNA capping enzyme alpha subunit (mRNA guanylyltransferase)
(GTP--RNA guanylyltransferase) (GTase)
Length = 402
Score = 36.2 bits (82), Expect = 0.024
Identities = 15/42 (35%), Positives = 28/42 (66%), Gaps = 3/42 (7%)
Frame = +3
Query: 3 PKSPEEHYTNTLLDGEMVIDTY---RGQKFPRFLIYDIMNIN 119
P + + T++DGE+V+DT G+K PRFL++D++ ++
Sbjct: 108 PNDIQAFHRGTIIDGELVMDTVPGTNGRKEPRFLVFDLLALD 149
>sp|Q6BT58|MCE1_DEBHA mRNA capping enzyme alpha subunit (mRNA guanylyltransferase)
(GTP--RNA guanylyltransferase) (GTase)
Length = 458
Score = 31.6 bits (70), Expect = 0.60
Identities = 22/68 (32%), Positives = 35/68 (51%), Gaps = 1/68 (1%)
Frame = +3
Query: 21 HYTNTLLDGEMVIDTYR-GQKFPRFLIYDIMNINGDYVGDKLFTLRHKLIKTEIIDTRTS 197
++ TLLDGE+V++T + RF I+D + ING K T RH + R
Sbjct: 116 YHHGTLLDGELVLETKNVPEPVLRFCIFDALAING-----KDITKRH-------LPKRLG 163
Query: 198 FVTPEMRK 221
++T ++ K
Sbjct: 164 YITEQVMK 171
>sp|Q01159|MCE1_YEAST mRNA capping enzyme alpha subunit (mRNA guanylyltransferase)
(GTP--RNA guanylyltransferase) (GTase)
Length = 459
Score = 29.6 bits (65), Expect = 2.3
Identities = 16/32 (50%), Positives = 21/32 (65%), Gaps = 2/32 (6%)
Frame = +3
Query: 33 TLLDGEMVIDTYRGQKFP--RFLIYDIMNING 122
TLLDGE+VI T K R+L++D + ING
Sbjct: 127 TLLDGELVIQTNPMTKLQELRYLMFDCLAING 158
>sp|P78587|MCE1_CANAL mRNA capping enzyme alpha subunit (mRNA guanylyltransferase)
(GTP--RNA guanylyltransferase) (GTase)
Length = 449
Score = 28.9 bits (63), Expect = 3.9
Identities = 20/83 (24%), Positives = 41/83 (49%), Gaps = 3/83 (3%)
Frame = +3
Query: 6 KSPEEHYTNTLLDGEMVIDTYR-GQKFPRFLIYDIMNINGDYVGDKLFTLRHKLIKTEII 182
+ P H+ TLLDGE+V++ + R++I+D + I+G + D+ R I ++
Sbjct: 113 EKPTYHH-GTLLDGELVLENRNVSEPVLRYVIFDALAIHGKCIIDRPLPKRLGYITENVM 171
Query: 183 DTRTSFV--TPEMRKNESFGIRI 245
+F P++ + F ++
Sbjct: 172 KPFDNFKKHNPDIVNSPEFPFKV 194
>sp|Q59940|IDH_STRMU Isocitrate dehydrogenase [NADP] (Oxalosuccinate decarboxylase)
(IDH) (NADP(+)-specific ICDH) (IDP)
Length = 393
Score = 28.5 bits (62), Expect = 5.1
Identities = 15/51 (29%), Positives = 22/51 (43%), Gaps = 8/51 (15%)
Frame = +3
Query: 15 EEHYTNTLLDGEMVIDTYRGQKF--------PRFLIYDIMNINGDYVGDKL 143
+ Y L G++V+D F RF + + N+NGDY D L
Sbjct: 239 KREYAAELASGQLVVDDIIADNFLQQILLKPERFDVVALTNLNGDYASDAL 289
>sp|Q9Z1G4|VPP1_MOUSE Vacuolar proton translocating ATPase 116 kDa subunit a isoform 1
(V-ATPase 116-kDa isoform a1) (Clathrin-coated
vesicle/synaptic vesicle proton pump 116 kDa subunit)
(Vacuolar proton pump subunit 1) (Vacuolar adenosine
triphosphatase subunit Ac116)
Length = 839
Score = 27.7 bits (60), Expect = 8.7
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +1
Query: 55 LSTHTEDRNSHDFSFTT**ISMAITLVINCSHCVTN 162
LSTH+ED DF T + AI + C C++N
Sbjct: 703 LSTHSEDAEEFDFGDTM--VHQAIHTIEYCLGCISN 736
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39,434,351
Number of Sequences: 369166
Number of extensions: 750810
Number of successful extensions: 1794
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1769
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1792
length of database: 68,354,980
effective HSP length: 69
effective length of database: 55,608,265
effective search space used: 1723856215
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)