Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_G23
(581 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q15629|TRAM1_HUMAN Translocation associated membrane pro... 175 6e-44
sp|Q91V04|TRAM1_MOUSE Translocation associated membrane pro... 174 1e-43
sp|Q01685|TRAM1_CANFA Translocation associated membrane pro... 173 3e-43
sp|Q9GKZ4|TRAM1_BOVIN Translocation associated membrane pro... 173 3e-43
sp|Q15035|TRAM2_HUMAN Translocation associated membrane pro... 150 2e-36
sp|Q924Z5|TRAM2_MOUSE Translocation associated membrane pro... 148 8e-36
sp|Q6ZMG9|LASS6_HUMAN LAG1 longevity assurance homolog 6 50 4e-06
sp|Q8C172|LASS6_MOUSE LAG1 longevity assurance homolog 6 48 2e-05
sp|Q8N5B7|LASS5_HUMAN LAG1 longevity assurance homolog 5 47 3e-05
sp|Q9HA82|LASS4_HUMAN LAG1 longevity assurance homolog 4 43 7e-04
>sp|Q15629|TRAM1_HUMAN Translocation associated membrane protein 1
Length = 374
Score = 175 bits (444), Expect = 6e-44
Identities = 85/179 (47%), Positives = 120/179 (67%)
Frame = +2
Query: 14 LWESYPNIPLTFWVKFYFIFQICYWLHNYPELYFQKVKRDEMPARVLYTTLYLFAVLMPY 193
LW +YP+ +TF +KF++I Q+ YWLH +PELYFQK K++++P +++Y LYLF + Y
Sbjct: 152 LWRAYPHNLMTFQMKFFYISQLAYWLHAFPELYFQKTKKEDIPRQLVYIGLYLFHIAGAY 211
Query: 194 VLNMTRLGLVLLVANYIVEFIFHISRLVYFTGKVKYAKIGFLLWDGLFLATRVLCTALAF 373
+LN+ LGLVLLV +Y VEF+FHISRL YF+ + KY K GF LW LF+ R+L L+
Sbjct: 212 LLNLNHLGLVLLVLHYFVEFLFHISRLFYFSNE-KYQK-GFSLWAVLFVLGRLLTLILSV 269
Query: 374 LYFHVGLEQKNQPTIKIAEGNFNTKIVRLNGLVFIFVTQLYAMWNFLNFHMKRWREKAS 550
L GL + + + GNFN VR+ L I VTQ + MW F+NF ++RWRE ++
Sbjct: 270 LTVGFGLARAENQKLDFSTGNFNVLAVRIAVLASICVTQAFMMWKFINFQLRRWREHSA 328
>sp|Q91V04|TRAM1_MOUSE Translocation associated membrane protein 1
Length = 374
Score = 174 bits (441), Expect = 1e-43
Identities = 84/179 (46%), Positives = 119/179 (66%)
Frame = +2
Query: 14 LWESYPNIPLTFWVKFYFIFQICYWLHNYPELYFQKVKRDEMPARVLYTTLYLFAVLMPY 193
LW +YP+ +TF KF++I Q+ YWLH +PELYFQK K++++P +++Y LYLF + Y
Sbjct: 152 LWRAYPHNLMTFQTKFFYISQLAYWLHAFPELYFQKTKKEDIPRQLVYIGLYLFHIAGAY 211
Query: 194 VLNMTRLGLVLLVANYIVEFIFHISRLVYFTGKVKYAKIGFLLWDGLFLATRVLCTALAF 373
+LN+ LGLVLLV +Y VEF+FHISRL YF+ + KY K GF LW LF+ R+L L+
Sbjct: 212 LLNLNHLGLVLLVLHYFVEFLFHISRLFYFSDE-KYQK-GFSLWAVLFVLGRLLTLILSV 269
Query: 374 LYFHVGLEQKNQPTIKIAEGNFNTKIVRLNGLVFIFVTQLYAMWNFLNFHMKRWREKAS 550
L GL + + + GNFN VR+ L I +TQ + MW F+NF ++RWRE ++
Sbjct: 270 LTVGFGLARAENQKLDFSTGNFNVLAVRIAVLASICITQAFMMWKFINFQLRRWREHSA 328
>sp|Q01685|TRAM1_CANFA Translocation associated membrane protein 1
Length = 374
Score = 173 bits (438), Expect = 3e-43
Identities = 83/179 (46%), Positives = 119/179 (66%)
Frame = +2
Query: 14 LWESYPNIPLTFWVKFYFIFQICYWLHNYPELYFQKVKRDEMPARVLYTTLYLFAVLMPY 193
LW +YP+ +TF +KF++I Q+ YW H +PELYFQK K++++P +++Y LYLF + Y
Sbjct: 152 LWRAYPHNLMTFQMKFFYIAQLAYWFHAFPELYFQKTKKEDIPRQLVYIGLYLFHIAGAY 211
Query: 194 VLNMTRLGLVLLVANYIVEFIFHISRLVYFTGKVKYAKIGFLLWDGLFLATRVLCTALAF 373
+LN+ LGLVLLV +Y VEF+FHISRL YF+ + KY K GF LW LF+ R+L L+
Sbjct: 212 LLNLNHLGLVLLVLHYFVEFLFHISRLFYFSDE-KYQK-GFSLWAVLFVLGRLLTLILSV 269
Query: 374 LYFHVGLEQKNQPTIKIAEGNFNTKIVRLNGLVFIFVTQLYAMWNFLNFHMKRWREKAS 550
L GL + + + GNFN VR+ L I +TQ + MW F+NF ++RWRE ++
Sbjct: 270 LTVGFGLARAENQKLDFSAGNFNVLAVRIAVLASICITQAFMMWKFINFQLRRWREHST 328
>sp|Q9GKZ4|TRAM1_BOVIN Translocation associated membrane protein 1
Length = 358
Score = 173 bits (438), Expect = 3e-43
Identities = 83/179 (46%), Positives = 119/179 (66%)
Frame = +2
Query: 14 LWESYPNIPLTFWVKFYFIFQICYWLHNYPELYFQKVKRDEMPARVLYTTLYLFAVLMPY 193
LW +YP+ +TF +KF++I Q+ YW H +PELYFQK K++++P +++Y LYLF + Y
Sbjct: 136 LWRAYPHNLMTFQMKFFYISQLAYWFHAFPELYFQKTKKEDIPRQLVYIGLYLFHIAGAY 195
Query: 194 VLNMTRLGLVLLVANYIVEFIFHISRLVYFTGKVKYAKIGFLLWDGLFLATRVLCTALAF 373
+LN+ LGLVLLV +Y VEF+FHISRL YF+ + KY K GF LW LF+ R+L L+
Sbjct: 196 LLNLNHLGLVLLVLHYFVEFLFHISRLFYFSDE-KYQK-GFSLWAVLFVLGRLLTLILSV 253
Query: 374 LYFHVGLEQKNQPTIKIAEGNFNTKIVRLNGLVFIFVTQLYAMWNFLNFHMKRWREKAS 550
L GL + + + GNFN VR+ L I +TQ + MW F+NF ++RWRE ++
Sbjct: 254 LTVGFGLARAENQKLDFSTGNFNVLAVRIAVLASICITQAFMMWKFINFQLRRWREHSA 312
>sp|Q15035|TRAM2_HUMAN Translocation associated membrane protein 2
Length = 370
Score = 150 bits (380), Expect = 2e-36
Identities = 73/176 (41%), Positives = 106/176 (60%)
Frame = +2
Query: 14 LWESYPNIPLTFWVKFYFIFQICYWLHNYPELYFQKVKRDEMPARVLYTTLYLFAVLMPY 193
LWE YP++ L F VKF+++ Q+ YWLH PELYFQKV+++E+P ++ Y LYL + Y
Sbjct: 147 LWEDYPHVHLPFQVKFFYLCQLAYWLHALPELYFQKVRKEEIPRQLQYICLYLVHIAGAY 206
Query: 194 VLNMTRLGLVLLVANYIVEFIFHISRLVYFTGKVKYAKIGFLLWDGLFLATRVLCTALAF 373
+LN++RLGL+LL+ Y EF+FH +RL YF + K+ F W +F TR+ LA
Sbjct: 207 LLNLSRLGLILLLLQYSTEFLFHTARLFYFADE-NNEKL-FSAWAAVFGVTRLFILTLAV 264
Query: 374 LYFHVGLEQKNQPTIKIAEGNFNTKIVRLNGLVFIFVTQLYAMWNFLNFHMKRWRE 541
L GL + +GNFNT RL L+ + Q + MW F++ ++ WRE
Sbjct: 265 LAIGFGLARMENQAFDPEKGNFNTLFCRLCVLLLVCAAQAWLMWRFIHSQLRHWRE 320
>sp|Q924Z5|TRAM2_MOUSE Translocation associated membrane protein 2
Length = 370
Score = 148 bits (374), Expect = 8e-36
Identities = 71/176 (40%), Positives = 107/176 (60%)
Frame = +2
Query: 14 LWESYPNIPLTFWVKFYFIFQICYWLHNYPELYFQKVKRDEMPARVLYTTLYLFAVLMPY 193
LWE YP++ L+F VKF+++ Q+ YWLH+ PELYFQKV+++E+P ++ Y LYL + Y
Sbjct: 147 LWEDYPHVYLSFQVKFFYLGQLAYWLHSLPELYFQKVRKEEVPRQLQYICLYLLHITGAY 206
Query: 194 VLNMTRLGLVLLVANYIVEFIFHISRLVYFTGKVKYAKIGFLLWDGLFLATRVLCTALAF 373
+LN++RLGL+LL+ Y E +FH++RL +F + F W +F TR+ LA
Sbjct: 207 LLNLSRLGLILLLLQYSTEALFHMARLFHFADENNERL--FNAWAAVFGVTRLFILTLAV 264
Query: 374 LYFHVGLEQKNQPTIKIAEGNFNTKIVRLNGLVFIFVTQLYAMWNFLNFHMKRWRE 541
L GL + +GNFNT RL L+ + V Q + MW F++ ++ WRE
Sbjct: 265 LTIGFGLARVENQVFDPEKGNFNTLPCRLGMLLLVCVAQAWLMWRFIHSQLRHWRE 320
>sp|Q6ZMG9|LASS6_HUMAN LAG1 longevity assurance homolog 6
Length = 384
Score = 50.1 bits (118), Expect = 4e-06
Identities = 27/113 (23%), Positives = 58/113 (51%), Gaps = 1/113 (0%)
Frame = +2
Query: 17 WESYPNIPLTFWVKFYFIFQIC-YWLHNYPELYFQKVKRDEMPARVLYTTLYLFAVLMPY 193
W +YP PLT + +Y+I ++ YW + + F +KR + L+ + +F + Y
Sbjct: 166 WYNYPYQPLTTDLHYYYILELSFYWSLMFSQ--FTDIKRKDFGIMFLHHLVSIFLITFSY 223
Query: 194 VLNMTRLGLVLLVANYIVEFIFHISRLVYFTGKVKYAKIGFLLWDGLFLATRV 352
V NM R+G ++L + + + +++ + K + F+++ +F+ TR+
Sbjct: 224 VNNMARVGTLVLCLHDSADALLEAAKMANYAKFQKMCDLLFVMFAVVFITTRL 276
>sp|Q8C172|LASS6_MOUSE LAG1 longevity assurance homolog 6
Length = 384
Score = 47.8 bits (112), Expect = 2e-05
Identities = 27/113 (23%), Positives = 56/113 (49%), Gaps = 1/113 (0%)
Frame = +2
Query: 17 WESYPNIPLTFWVKFYFIFQIC-YWLHNYPELYFQKVKRDEMPARVLYTTLYLFAVLMPY 193
W +YP PLT + +Y+I ++ YW + F +KR + L+ +F + Y
Sbjct: 166 WYNYPYQPLTADLHYYYILELSFYWSLMVSQ--FTDIKRKDFGIMFLHHLATIFLITFSY 223
Query: 194 VLNMTRLGLVLLVANYIVEFIFHISRLVYFTGKVKYAKIGFLLWDGLFLATRV 352
V NM R+G ++L + + + +++ + K + F+++ +F+ TR+
Sbjct: 224 VNNMARVGTLVLCLHDSADALLEAAKMANYAKFQKMCDLLFVMFAVVFITTRL 276
>sp|Q8N5B7|LASS5_HUMAN LAG1 longevity assurance homolog 5
Length = 392
Score = 47.0 bits (110), Expect = 3e-05
Identities = 23/113 (20%), Positives = 57/113 (50%), Gaps = 1/113 (0%)
Frame = +2
Query: 17 WESYPNIPLTFWVKFYFIFQIC-YWLHNYPELYFQKVKRDEMPARVLYTTLYLFAVLMPY 193
W +YP PL+ + Y+I ++ YW + + F +KR + ++ + + + Y
Sbjct: 175 WHNYPFQPLSSGLYHYYIMELAFYWSLMFSQ--FTDIKRKDFLIMFVHHLVTIGLISFSY 232
Query: 194 VLNMTRLGLVLLVANYIVEFIFHISRLVYFTGKVKYAKIGFLLWDGLFLATRV 352
+ NM R+G +++ + + +F+ ++L + + F+++ +F+ TR+
Sbjct: 233 INNMVRVGTLIMCLHDVSDFLLEAAKLANYAKYQRLCDTLFVIFSAVFMVTRL 285
>sp|Q9HA82|LASS4_HUMAN LAG1 longevity assurance homolog 4
Length = 394
Score = 42.7 bits (99), Expect = 7e-04
Identities = 25/121 (20%), Positives = 60/121 (49%)
Frame = +2
Query: 17 WESYPNIPLTFWVKFYFIFQICYWLHNYPELYFQKVKRDEMPARVLYTTLYLFAVLMPYV 196
W+ YPN L + ++++ ++ ++L L F VKR + +V++ + + + Y
Sbjct: 167 WDRYPNQTLKPSLYWWYLLELGFYLSLLIRLPFD-VKRKDFKEQVIHHFVAVILMTFSYS 225
Query: 197 LNMTRLGLVLLVANYIVEFIFHISRLVYFTGKVKYAKIGFLLWDGLFLATRVLCTALAFL 376
N+ R+G ++L+ + +++ ++V + + FL++ +F TR++ L
Sbjct: 226 ANLLRIGSLVLLLHDSSDYLLEACKMVNYMQYQQVCDALFLIFSFVFFYTRLVLFPTQIL 285
Query: 377 Y 379
Y
Sbjct: 286 Y 286
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 69,836,894
Number of Sequences: 369166
Number of extensions: 1403942
Number of successful extensions: 3578
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3471
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3564
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4308286840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)