Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_G09
(920 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O00418|EF2K_HUMAN Elongation factor 2 kinase (eEF-2 kina... 83 1e-15
sp|P70531|EF2K_RAT Elongation factor 2 kinase (eEF-2 kinase... 80 1e-14
sp|O08796|EF2K_MOUSE Elongation factor 2 kinase (eEF-2 kina... 79 1e-14
sp|O01991|EF2K_CAEEL Elongation factor 2 kinase (eEF-2 kina... 51 5e-06
sp|Q9DCV6|K0141_MOUSE Protein KIAA0141 31 5.6
>sp|O00418|EF2K_HUMAN Elongation factor 2 kinase (eEF-2 kinase) (eEF-2K)
(Calcium/calmodulin-dependent eukaryotic elongation
factor 2 kinase)
Length = 725
Score = 82.8 bits (203), Expect = 1e-15
Identities = 54/181 (29%), Positives = 81/181 (44%)
Frame = +3
Query: 324 NLDQEIGHSILAQVHHELARLHESGRFIKSKQLAAGCLHHQFWSHLLNENDSPEAIVKET 503
+L+++IG SIL +VH + R HE GRF C + W
Sbjct: 513 DLEKKIGKSILGKVHLAMVRYHEGGRF---------CEKGEEWDQ--------------- 548
Query: 504 SNDINWESCLFHERHAAQLGCLEAVIVMARYCLNMSGHMLQDCPFPPTQNDLDCGLDYLW 683
ES +FH HAA LG LEA++ + + H+L D T+ + G DYL
Sbjct: 549 ------ESAVFHLEHAANLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLL 602
Query: 684 RAADSGDRRSMIELANLYRKTENTEEKTYEGNIMKTLLDVNWYEAVKWYKRAFEAANKPD 863
+AA++GDR+SMI +A + +N + +W EA+ WY A E + +
Sbjct: 603 KAAEAGDRQSMILVARAFDSGQNLSPDRCQ----------DWLEALHWYNTALEMTDCDE 652
Query: 864 G 866
G
Sbjct: 653 G 653
>sp|P70531|EF2K_RAT Elongation factor 2 kinase (eEF-2 kinase) (eEF-2K)
(Calcium/calmodulin-dependent eukaryotic elongation
factor 2 kinase)
Length = 724
Score = 79.7 bits (195), Expect = 1e-14
Identities = 53/181 (29%), Positives = 79/181 (43%)
Frame = +3
Query: 324 NLDQEIGHSILAQVHHELARLHESGRFIKSKQLAAGCLHHQFWSHLLNENDSPEAIVKET 503
+L ++IG S+L +VH + R HE GRF C + W
Sbjct: 512 DLGRKIGKSVLGKVHLAMVRYHEGGRF---------CEKDEEWDQ--------------- 547
Query: 504 SNDINWESCLFHERHAAQLGCLEAVIVMARYCLNMSGHMLQDCPFPPTQNDLDCGLDYLW 683
ES +FH HAA LG LEA++ + + H+L D T+ + G DYL
Sbjct: 548 ------ESAIFHLEHAADLGELEAIVGLGLMYSQLPHHILADVSLEETEENKTKGFDYLL 601
Query: 684 RAADSGDRRSMIELANLYRKTENTEEKTYEGNIMKTLLDVNWYEAVKWYKRAFEAANKPD 863
+AA++GDR+SMI +A + N + +W EA+ WY A E + +
Sbjct: 602 KAAEAGDRQSMILVARAFDTGLNLSPDRCQ----------DWSEALHWYNTALETTDCDE 651
Query: 864 G 866
G
Sbjct: 652 G 652
>sp|O08796|EF2K_MOUSE Elongation factor 2 kinase (eEF-2 kinase) (eEF-2K)
(Calcium/calmodulin-dependent eukaryotic elongation
factor 2 kinase)
Length = 724
Score = 79.3 bits (194), Expect = 1e-14
Identities = 53/181 (29%), Positives = 78/181 (43%)
Frame = +3
Query: 324 NLDQEIGHSILAQVHHELARLHESGRFIKSKQLAAGCLHHQFWSHLLNENDSPEAIVKET 503
+L ++IG S+L +VH + R HE GRF C + W
Sbjct: 512 DLGRKIGKSVLGKVHLAMVRYHEGGRF---------CEKDEEWDR--------------- 547
Query: 504 SNDINWESCLFHERHAAQLGCLEAVIVMARYCLNMSGHMLQDCPFPPTQNDLDCGLDYLW 683
ES +FH HAA LG LEA++ + + H+L D T+ + G DYL
Sbjct: 548 ------ESAIFHLEHAADLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLL 601
Query: 684 RAADSGDRRSMIELANLYRKTENTEEKTYEGNIMKTLLDVNWYEAVKWYKRAFEAANKPD 863
+AA++GDR SMI +A + N + +W EA+ WY A E + +
Sbjct: 602 KAAEAGDRHSMILVARAFDTGLNLSPDRCQ----------DWSEALHWYNTALETTDCDE 651
Query: 864 G 866
G
Sbjct: 652 G 652
>sp|O01991|EF2K_CAEEL Elongation factor 2 kinase (eEF-2 kinase) (eEF-2K)
(Calcium/calmodulin-dependent eukaryotic elongation
factor 2 kinase)
Length = 768
Score = 50.8 bits (120), Expect = 5e-06
Identities = 51/212 (24%), Positives = 95/212 (44%), Gaps = 26/212 (12%)
Frame = +3
Query: 279 SMRENQKPSSATHPTNLDQEIGH--SILAQVHHELARLHESGRFI----KSKQLAAGCLH 440
S++ Q ++ + ++ Q GH S+L Q+H +L+R HE GRF+ + K++ G
Sbjct: 500 SLQLQQMAANLENDEDVPQVTGHQFSVLGQIHIDLSRYHELGRFVEVDSEHKEMLEG--- 556
Query: 441 HQFWSHLLNENDSPEAIVKETSNDINWESCLFHERHAAQLGCLEAVIVMARYCLNMSGHM 620
+END+ I + +S +FH A + G LEAV+ A L + +
Sbjct: 557 --------SENDARVPIKYDK------QSAIFHLDIARKCGILEAVLTSAHIVLGLPHEL 602
Query: 621 LQDCP--------FPPTQNDL------------DCGLDYLWRAADSGDRRSMIELANLYR 740
L++ F +N + + G D + AA+ GD+ +M+ +A+ Y
Sbjct: 603 LKEVTVDDLFPNGFGEQENGIRADKGQKPCDLEEFGSDLMEIAAEMGDKGAMLYMAHAY- 661
Query: 741 KTENTEEKTYEGNIMKTLLDVNWYEAVKWYKR 836
E + G +T ++ +++ WY+R
Sbjct: 662 -----ETGQHLGPNRRT----DYKKSIDWYQR 684
>sp|Q9DCV6|K0141_MOUSE Protein KIAA0141
Length = 510
Score = 30.8 bits (68), Expect = 5.6
Identities = 20/62 (32%), Positives = 26/62 (41%)
Frame = +3
Query: 675 YLWRAADSGDRRSMIELANLYRKTENTEEKTYEGNIMKTLLDVNWYEAVKWYKRAFEAAN 854
YLW AA +GD +S L Y K + N EAVK Y++A N
Sbjct: 376 YLWLAASNGDSQSRFHLGICYEKGLGAQR--------------NLGEAVKCYQQAAAMGN 421
Query: 855 KP 860
+P
Sbjct: 422 EP 423
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 97,425,287
Number of Sequences: 369166
Number of extensions: 1860561
Number of successful extensions: 4484
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 4315
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4479
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9414689480
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)