Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_F19
(640 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q5M8Y1|SPCS2_XENTR Probable signal peptidase complex sub... 140 2e-33
sp|Q28250|SPCS2_CANFA Signal peptidase complex subunit 2 (M... 139 8e-33
sp|Q15005|SPCS2_HUMAN Signal peptidase complex subunit 2 (M... 139 8e-33
sp|Q5RAY6|SPCS2_PONPY Signal peptidase complex subunit 2 (M... 139 8e-33
sp|Q9CYN2|SPCS2_MOUSE Signal peptidase complex subunit 2 (M... 139 8e-33
sp|Q4R512|SPCS2_MACFA Signal peptidase complex subunit 2 (M... 139 8e-33
sp|Q5BJI9|SPCS2_BRARE Probable signal peptidase complex sub... 137 2e-32
sp|Q9VYY2|SPCS2_DROME Probable signal peptidase complex sub... 93 5e-19
sp|Q615A2|SPCS2_CAEBR Probable signal peptidase complex sub... 88 2e-17
sp|Q9XWW1|SPCS2_CAEEL Probable signal peptidase complex sub... 87 3e-17
>sp|Q5M8Y1|SPCS2_XENTR Probable signal peptidase complex subunit 2 (Microsomal signal
peptidase 25 kDa subunit) (SPase 25 kDa subunit)
Length = 201
Score = 140 bits (354), Expect = 2e-33
Identities = 69/162 (42%), Positives = 101/162 (62%)
Frame = +2
Query: 32 DTNATKNALDDGFKKILKEQYKFEEKFALIDGRLLISTVAVLCGGVAVLYDWFYPFPASK 211
D +A KN+LDD KK+L E+Y++ E F LIDGRL+I T++ + VA+++D+ +PFP SK
Sbjct: 27 DGSAVKNSLDDAAKKVLLEKYRYVENFCLIDGRLIICTISCVFAIVALVWDYLHPFPESK 86
Query: 212 LVLIVCVLLYFALTGLLTCYIMFVERSTFFLAHENDTAGLDKPIKWNFPSKMKKYQDKYE 391
VL +CV+ YF + G+LT Y + E+S F +AH D AG+D W+ S +K++ DKY
Sbjct: 87 PVLAICVISYFLMMGILTIYTSYKEKSIFLVAHRKDPAGMDPDDIWHLSSSLKRFDDKYT 146
Query: 392 LTIKREDLNAKQSHLMTESQSIAEFFDIKGNLRMDILEKYVT 517
L + K ++SIA FFD G L MD+ E V+
Sbjct: 147 LKVTYISGKTKAQRDAEFTKSIARFFDDNGTLVMDLFEPEVS 188
>sp|Q28250|SPCS2_CANFA Signal peptidase complex subunit 2 (Microsomal signal peptidase 25
kDa subunit) (SPase 25 kDa subunit)
Length = 226
Score = 139 bits (349), Expect = 8e-33
Identities = 69/162 (42%), Positives = 98/162 (60%)
Frame = +2
Query: 32 DTNATKNALDDGFKKILKEQYKFEEKFALIDGRLLISTVAVLCGGVAVLYDWFYPFPASK 211
D +A KN+LDD KK+L E+YK+ E F LIDGRL I T++ VA+++D+ +PFP SK
Sbjct: 52 DGSAVKNSLDDSAKKVLLEKYKYVENFGLIDGRLTICTISCFFAIVALIWDYMHPFPESK 111
Query: 212 LVLIVCVLLYFALTGLLTCYIMFVERSTFFLAHENDTAGLDKPIKWNFPSKMKKYQDKYE 391
VL +CV+ YF + G+LT Y + E+S F +AH D G+D W S +K++ DKY
Sbjct: 112 PVLALCVISYFVMMGILTIYTSYKEKSIFLVAHRKDPTGMDPDDIWQLSSSLKRFDDKYT 171
Query: 392 LTIKREDLNAKQSHLMTESQSIAEFFDIKGNLRMDILEKYVT 517
L + KQ ++SIA+FFD G L MD E ++
Sbjct: 172 LKLTFISGRTKQQREAEFTKSIAKFFDHSGTLVMDAYEPEIS 213
>sp|Q15005|SPCS2_HUMAN Signal peptidase complex subunit 2 (Microsomal signal peptidase 25
kDa subunit) (SPase 25 kDa subunit)
Length = 226
Score = 139 bits (349), Expect = 8e-33
Identities = 69/162 (42%), Positives = 98/162 (60%)
Frame = +2
Query: 32 DTNATKNALDDGFKKILKEQYKFEEKFALIDGRLLISTVAVLCGGVAVLYDWFYPFPASK 211
D +A KN+LDD KK+L E+YK+ E F LIDGRL I T++ VA+++D+ +PFP SK
Sbjct: 52 DGSAVKNSLDDSAKKVLLEKYKYVENFGLIDGRLTICTISCFFAIVALIWDYMHPFPESK 111
Query: 212 LVLIVCVLLYFALTGLLTCYIMFVERSTFFLAHENDTAGLDKPIKWNFPSKMKKYQDKYE 391
VL +CV+ YF + G+LT Y + E+S F +AH D G+D W S +K++ DKY
Sbjct: 112 PVLALCVISYFVMMGILTIYTSYKEKSIFLVAHRKDPTGMDPDDIWQLSSSLKRFDDKYT 171
Query: 392 LTIKREDLNAKQSHLMTESQSIAEFFDIKGNLRMDILEKYVT 517
L + KQ ++SIA+FFD G L MD E ++
Sbjct: 172 LKLTFISGRTKQQREAEFTKSIAKFFDHSGTLVMDAYEPEIS 213
>sp|Q5RAY6|SPCS2_PONPY Signal peptidase complex subunit 2 (Microsomal signal peptidase 25
kDa subunit) (SPase 25 kDa subunit)
Length = 226
Score = 139 bits (349), Expect = 8e-33
Identities = 69/162 (42%), Positives = 98/162 (60%)
Frame = +2
Query: 32 DTNATKNALDDGFKKILKEQYKFEEKFALIDGRLLISTVAVLCGGVAVLYDWFYPFPASK 211
D +A KN+LDD KK+L E+YK+ E F LIDGRL I T++ VA+++D+ +PFP SK
Sbjct: 52 DGSAVKNSLDDSAKKVLLEKYKYVENFGLIDGRLTICTISCFFAIVALIWDYMHPFPESK 111
Query: 212 LVLIVCVLLYFALTGLLTCYIMFVERSTFFLAHENDTAGLDKPIKWNFPSKMKKYQDKYE 391
VL +CV+ YF + G+LT Y + E+S F +AH D G+D W S +K++ DKY
Sbjct: 112 PVLALCVISYFVMMGILTIYTSYKEKSIFLVAHRKDPTGMDPDDIWQLSSSLKRFDDKYT 171
Query: 392 LTIKREDLNAKQSHLMTESQSIAEFFDIKGNLRMDILEKYVT 517
L + KQ ++SIA+FFD G L MD E ++
Sbjct: 172 LKLTFISGRTKQQREAEFTKSIAKFFDHSGTLVMDAYEPEIS 213
>sp|Q9CYN2|SPCS2_MOUSE Signal peptidase complex subunit 2 (Microsomal signal peptidase 25
kDa subunit) (SPase 25 kDa subunit)
Length = 226
Score = 139 bits (349), Expect = 8e-33
Identities = 69/162 (42%), Positives = 98/162 (60%)
Frame = +2
Query: 32 DTNATKNALDDGFKKILKEQYKFEEKFALIDGRLLISTVAVLCGGVAVLYDWFYPFPASK 211
D +A KN+LDD KK+L E+YK+ E F LIDGRL I T++ VA+++D+ +PFP SK
Sbjct: 52 DGSAVKNSLDDSAKKVLLEKYKYVENFGLIDGRLTICTISCFFAIVALIWDYMHPFPESK 111
Query: 212 LVLIVCVLLYFALTGLLTCYIMFVERSTFFLAHENDTAGLDKPIKWNFPSKMKKYQDKYE 391
VL +CV+ YF + G+LT Y + E+S F +AH D G+D W S +K++ DKY
Sbjct: 112 PVLALCVISYFVMMGILTIYTSYKEKSIFLVAHRKDPTGMDPDDIWQLSSSLKRFDDKYT 171
Query: 392 LTIKREDLNAKQSHLMTESQSIAEFFDIKGNLRMDILEKYVT 517
L + KQ ++SIA+FFD G L MD E ++
Sbjct: 172 LKLTFISGRTKQQREAEFTKSIAKFFDHSGTLVMDAYEPEIS 213
>sp|Q4R512|SPCS2_MACFA Signal peptidase complex subunit 2 (Microsomal signal peptidase 25
kDa subunit) (SPase 25 kDa subunit)
Length = 226
Score = 139 bits (349), Expect = 8e-33
Identities = 69/162 (42%), Positives = 98/162 (60%)
Frame = +2
Query: 32 DTNATKNALDDGFKKILKEQYKFEEKFALIDGRLLISTVAVLCGGVAVLYDWFYPFPASK 211
D +A KN+LDD KK+L E+YK+ E F LIDGRL I T++ VA+++D+ +PFP SK
Sbjct: 52 DGSAVKNSLDDSAKKVLLEKYKYVENFGLIDGRLTICTISCFFAIVALIWDYMHPFPESK 111
Query: 212 LVLIVCVLLYFALTGLLTCYIMFVERSTFFLAHENDTAGLDKPIKWNFPSKMKKYQDKYE 391
VL +CV+ YF + G+LT Y + E+S F +AH D G+D W S +K++ DKY
Sbjct: 112 PVLALCVISYFVMMGILTIYTSYKEKSIFLVAHRKDPTGMDPDDIWQLSSSLKRFDDKYT 171
Query: 392 LTIKREDLNAKQSHLMTESQSIAEFFDIKGNLRMDILEKYVT 517
L + KQ ++SIA+FFD G L MD E ++
Sbjct: 172 LKLTFISGRTKQQREAEFTKSIAKFFDHSGTLVMDAYEPEIS 213
>sp|Q5BJI9|SPCS2_BRARE Probable signal peptidase complex subunit 2 (Microsomal signal
peptidase 25 kDa subunit) (SPase 25 kDa subunit)
Length = 201
Score = 137 bits (346), Expect = 2e-32
Identities = 72/168 (42%), Positives = 102/168 (60%)
Frame = +2
Query: 32 DTNATKNALDDGFKKILKEQYKFEEKFALIDGRLLISTVAVLCGGVAVLYDWFYPFPASK 211
D A KN+LDD KK+L E+Y + E F L+DGRL I TV+ L VA+++D+ +PFP SK
Sbjct: 27 DGAAVKNSLDDAAKKVLIEKYGYLESFNLVDGRLFICTVSCLFTIVALIWDYLHPFPESK 86
Query: 212 LVLIVCVLLYFALTGLLTCYIMFVERSTFFLAHENDTAGLDKPIKWNFPSKMKKYQDKYE 391
VL CV+ YF + G+LT Y + E++ F +A + D AG+D W S +K++ D+Y
Sbjct: 87 PVLACCVVSYFIMMGILTLYTSYKEKNIFLVAMQKDPAGMDPDHSWCLSSSLKRFDDQYT 146
Query: 392 LTIKREDLNAKQSHLMTESQSIAEFFDIKGNLRMDILEKYVTNPKTTL 535
L + D KQS ++S++ FFD G L MD EKYV+ TL
Sbjct: 147 LRMSFTDGKTKQSRETEFTKSVSVFFDENGTLVMDQYEKYVSKLHDTL 194
>sp|Q9VYY2|SPCS2_DROME Probable signal peptidase complex subunit 2 (Microsomal signal
peptidase 25 kDa subunit) (SPase 25 kDa subunit)
Length = 199
Score = 93.2 bits (230), Expect = 5e-19
Identities = 55/151 (36%), Positives = 86/151 (56%), Gaps = 1/151 (0%)
Frame = +2
Query: 32 DTNATKNALDDGFKK-ILKEQYKFEEKFALIDGRLLISTVAVLCGGVAVLYDWFYPFPAS 208
D +A K+ALDD K +L ++ + +E+F L++ RL + +AV +A +D+ +PFP S
Sbjct: 21 DGSAVKHALDDAVKTCLLGDRPQLKEQFGLVNTRLALCALAVSVAIMAHAWDFTHPFPES 80
Query: 209 KLVLIVCVLLYFALTGLLTCYIMFVERSTFFLAHENDTAGLDKPIKWNFPSKMKKYQDKY 388
+ VL+ VL YFAL G+LT + F E+ TF +A + D ++ W S M+KY DKY
Sbjct: 81 RPVLLFSVLAYFALLGILTLHSSFREKGTFAVALQKDK---ERERLWEASSDMRKYDDKY 137
Query: 389 ELTIKREDLNAKQSHLMTESQSIAEFFDIKG 481
LT+ D + + ++S A F D G
Sbjct: 138 LLTLSVRDTKNGKRREQSSNKSCAAFIDQNG 168
>sp|Q615A2|SPCS2_CAEBR Probable signal peptidase complex subunit 2 (Microsomal signal
peptidase 25 kDa subunit) (SPase 25 kDa subunit)
Length = 180
Score = 88.2 bits (217), Expect = 2e-17
Identities = 53/162 (32%), Positives = 93/162 (57%), Gaps = 1/162 (0%)
Frame = +2
Query: 32 DTNATKNALDDGFKKILKEQYKFEEKFALIDGRLLISTVAVLCGGVAVLYDWFYPFPASK 211
D KN LD+ KKIL ++ + E+ L++ RLLIS + V A YD++ PFP SK
Sbjct: 13 DGPTVKNGLDEVVKKILNDKVGWTEQHNLMNLRLLISFIGVAFSAFACGYDFYAPFPKSK 72
Query: 212 LVLIVCVLLYFALTGLLTCYIMFVERSTFFLAHENDTAGLDKPIKWNFPSKMKKYQDKYE 391
+VL+VC + YF G+L + +VE+ F+ A+E D + KW + S++K + DKY
Sbjct: 73 IVLLVCSVSYFICMGVLQLFQWYVEKDCFYEANEVDG---KQTRKWAWSSEIKAHDDKYV 129
Query: 392 LTIK-REDLNAKQSHLMTESQSIAEFFDIKGNLRMDILEKYV 514
L+ + +++ + Q ++ +SI + D G + + ++++ V
Sbjct: 130 LSAEFKKEGRSGQGKII---KSIGAYIDNDGEIMIPLVQREV 168
>sp|Q9XWW1|SPCS2_CAEEL Probable signal peptidase complex subunit 2 (Microsomal signal
peptidase 25 kDa subunit) (SPase 25 kDa subunit)
Length = 180
Score = 87.4 bits (215), Expect = 3e-17
Identities = 56/161 (34%), Positives = 86/161 (53%)
Frame = +2
Query: 32 DTNATKNALDDGFKKILKEQYKFEEKFALIDGRLLISTVAVLCGGVAVLYDWFYPFPASK 211
D KNALD+ KKIL ++ + E L++ RLLIS + V A YD++ PFP SK
Sbjct: 13 DGPTVKNALDEVVKKILNDKVGWTESHNLMNLRLLISFIGVAFSAFACGYDYYEPFPKSK 72
Query: 212 LVLIVCVLLYFALTGLLTCYIMFVERSTFFLAHENDTAGLDKPIKWNFPSKMKKYQDKYE 391
+VL VC + YF G+L Y +VE+ + A E D + KW + S++K + DKY
Sbjct: 73 IVLAVCSVSYFICMGILQMYQWYVEKDCIYEATEVDG---KQSRKWAWSSEIKAHDDKY- 128
Query: 392 LTIKREDLNAKQSHLMTESQSIAEFFDIKGNLRMDILEKYV 514
T+ E +S ++SI + D G + + +++K V
Sbjct: 129 -TLSAEFKKEGRSGQGKITKSIGAYIDNDGEIIVPLVKKEV 168
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 67,114,275
Number of Sequences: 369166
Number of extensions: 1224412
Number of successful extensions: 3740
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3625
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3732
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 5169945420
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)