Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_F03
(835 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9EQH3|VPS35_MOUSE Vacuolar protein sorting 35 (Vesicle ... 284 2e-76
sp|Q96QK1|VPS35_HUMAN Vacuolar protein sorting 35 (Vesicle ... 284 2e-76
sp|P34110|VPS35_YEAST Vacuolar protein sorting-associated p... 136 8e-32
sp|O74552|VPS35_SCHPO Vacuolar protein sorting-associated p... 132 9e-31
sp|Q58611|YC14_METJA Hypothetical protein MJ1214 34 0.43
sp|Q8TDY2|RBCC1_HUMAN RB1-inducible coiled-coil protein 1 34 0.57
sp|Q859W7|YCF2_ANTFO Protein ycf2 32 1.7
sp|O60566|BUB1B_HUMAN Mitotic checkpoint serine/threonine-p... 32 2.8
sp|Q61098|IL18R_MOUSE Interleukin-18 receptor 1 precursor (... 31 4.8
sp|Q8TMT1|DNLI_METAC DNA ligase (Polydeoxyribonucleotide sy... 30 6.3
>sp|Q9EQH3|VPS35_MOUSE Vacuolar protein sorting 35 (Vesicle protein sorting 35)
(Maternal-embryonic 3)
Length = 796
Score = 284 bits (727), Expect = 2e-76
Identities = 137/278 (49%), Positives = 198/278 (71%)
Frame = +2
Query: 2 FL*MNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVERYKTTVLPAV 181
F MNKLWVRMQHQGH+ VGTNL RLSQLE ++VERYK VL +
Sbjct: 187 FAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGI 246
Query: 182 LEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELEPNVHIKSIIGALIERL 361
LEQ+VNC DA+AQEYLM+ +IQVFPDEFHL+TL LRA +EL NV++K+II ALI+RL
Sbjct: 247 LEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRL 306
Query: 362 SQYATEESGTGIPDSIPLFDIFSREVNSMVERRKDLPPEDIMSLEASLLHLALKVYPNKL 541
+ +A E G GIP I LFDIFS++V ++++ R+D+P ED++SL+ SL++LA+K YP+++
Sbjct: 307 ALFAHREDGPGIPAEIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRV 366
Query: 542 ELVDNVVKGATEALRNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILKLENYSDLIQ 721
+ VD V++ E +N+ I+ SS +S+EL RLLK+P+D Y +I +LKL+++ L +
Sbjct: 367 DYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFE 426
Query: 722 NLEYNGRKLISCHLLTNTLDQNHVLNTEEQLDSLFKCI 835
+Y RK +SC++L+N LD N + +++Q+DS+ +
Sbjct: 427 YFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLV 464
>sp|Q96QK1|VPS35_HUMAN Vacuolar protein sorting 35 (Vesicle protein sorting 35) (hVPS35)
(Maternal-embryonic 3)
Length = 796
Score = 284 bits (727), Expect = 2e-76
Identities = 137/278 (49%), Positives = 198/278 (71%)
Frame = +2
Query: 2 FL*MNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVERYKTTVLPAV 181
F MNKLWVRMQHQGH+ VGTNL RLSQLE ++VERYK VL +
Sbjct: 187 FAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGI 246
Query: 182 LEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELEPNVHIKSIIGALIERL 361
LEQ+VNC DA+AQEYLM+ +IQVFPDEFHL+TL LRA +EL NV++K+II ALI+RL
Sbjct: 247 LEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRL 306
Query: 362 SQYATEESGTGIPDSIPLFDIFSREVNSMVERRKDLPPEDIMSLEASLLHLALKVYPNKL 541
+ +A E G GIP I LFDIFS++V ++++ R+D+P ED++SL+ SL++LA+K YP+++
Sbjct: 307 ALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRV 366
Query: 542 ELVDNVVKGATEALRNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILKLENYSDLIQ 721
+ VD V++ E +N+ I+ SS +S+EL RLLK+P+D Y +I +LKL+++ L +
Sbjct: 367 DYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFE 426
Query: 722 NLEYNGRKLISCHLLTNTLDQNHVLNTEEQLDSLFKCI 835
+Y RK +SC++L+N LD N + +++Q+DS+ +
Sbjct: 427 YFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLV 464
>sp|P34110|VPS35_YEAST Vacuolar protein sorting-associated protein VPS35
Length = 944
Score = 136 bits (342), Expect = 8e-32
Identities = 75/213 (35%), Positives = 116/213 (54%), Gaps = 7/213 (3%)
Frame = +2
Query: 2 FL*MNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVERYKTTVLPAV 181
F+ MNKLWVR+QHQG VG+ L RLSQ+ + + YK +LP +
Sbjct: 168 FIEMNKLWVRLQHQGPLRERETRTRERKELQILVGSQLVRLSQIIDDNFQMYKQDILPTI 227
Query: 182 LEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELEPNVHIKSIIGALIERL 361
LEQ++ C D ++QEYL+DV+ QVF DEFHL+TL+TLL+ T L P+V I I+ L++RL
Sbjct: 228 LEQVIQCRDLVSQEYLLDVICQVFADEFHLKTLDTLLQTTLHLNPDVSINKIVLTLVDRL 287
Query: 362 SQYATEE-------SGTGIPDSIPLFDIFSREVNSMVERRKDLPPEDIMSLEASLLHLAL 520
+ Y T + + T + +F F + + R DL + + L S++ L+L
Sbjct: 288 NDYVTRQLEDDPNATSTNAYLDMDVFGTFWDYLTVLNHERPDLSLQQFIPLVESVIVLSL 347
Query: 521 KVYPNKLELVDNVVKGATEALRNMNISKISYSS 619
K YPN + ++ + + + ++ IS S
Sbjct: 348 KWYPNNFDNLNKLFELVLQKTKDYGQKNISLES 380
>sp|O74552|VPS35_SCHPO Vacuolar protein sorting-associated protein vps35
Length = 785
Score = 132 bits (333), Expect = 9e-31
Identities = 88/309 (28%), Positives = 150/309 (48%), Gaps = 39/309 (12%)
Frame = +2
Query: 2 FL*MNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVERYKTTVLPAV 181
F MNKLWVR+QH G VG NL RLSQL ++D++ Y+ VLPA+
Sbjct: 137 FTEMNKLWVRIQHLGPIKEFSKRTQERNELKVLVGLNLVRLSQL-NLDIDTYRDHVLPAI 195
Query: 182 LEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELEPNVHIKSIIGALIERL 361
+EQI+ C D++AQEYL++V+ Q F D HL+TL+T +L P+V++ ++ A++ RL
Sbjct: 196 IEQIIECRDSLAQEYLVEVICQAFSDNMHLQTLDTYFGTVIKLSPSVNVTQLVVAMLNRL 255
Query: 362 SQY-----------------ATEESG-------------------TGIPDSIPLFDIFSR 433
+ Y TE+ G IP + ++
Sbjct: 256 TDYVQREYESDSSNEDESETVTEKLGDIKINEEVQQKDEQECPGDKVIPPEYAIQEVLWS 315
Query: 434 EVNSMVERRKDLPPEDIMSLEASLLHLALKVYPNKLELVDNVVKGATEALRNM-NISKIS 610
V +++ R LP + I+S+ +S+L+ L+ YP K + D V + E + N ++
Sbjct: 316 HVVEVIQSRSGLPLDCIVSILSSILNFFLRCYPYKPQYADRVFQYINEHIINQPSLRSAL 375
Query: 611 YSSCLSRELIRLLKMPIDVYKDIRIILKLENYSDLIQNLEYNGRKLISCHLLTNTLDQNH 790
+ L + L +L +P+ + L+L+N+ + + N R I+ ++ +++ H
Sbjct: 376 HERPLQKSLCAILLLPLTYFPSFSYCLELQNFLPVFNAQDPNLRYDIARMIVQKIIEKGH 435
Query: 791 VLN--TEEQ 811
L+ TE Q
Sbjct: 436 SLSELTEAQ 444
>sp|Q58611|YC14_METJA Hypothetical protein MJ1214
Length = 1018
Score = 34.3 bits (77), Expect = 0.43
Identities = 30/132 (22%), Positives = 62/132 (46%), Gaps = 1/132 (0%)
Frame = +2
Query: 419 DIFSREVN-SMVERRKDLPPEDIMSLEASLLHLALKVYPNKLELVDNVVKGATEALRNMN 595
DI S +N ++E K+ ++I + A +++ AL NK E + ++ L+N
Sbjct: 846 DIASTSINPQLLENLKN--KDEINVIVADMIYYALDTLQNKKEPIYRMIYDRINELKNAY 903
Query: 596 ISKISYSSCLSRELIRLLKMPIDVYKDIRIILKLENYSDLIQNLEYNGRKLISCHLLTNT 775
ISK + + ELI L ++ + + K E I+N+ + + + +C++
Sbjct: 904 ISKTKKNEYVINELINCLNALKTYEEEEKTLSKSEK---AIKNMLFYLKNVENCNIKKLP 960
Query: 776 LDQNHVLNTEEQ 811
L + + N E++
Sbjct: 961 LTEKTLKNLEDK 972
>sp|Q8TDY2|RBCC1_HUMAN RB1-inducible coiled-coil protein 1
Length = 1594
Score = 33.9 bits (76), Expect = 0.57
Identities = 51/208 (24%), Positives = 88/208 (42%), Gaps = 16/208 (7%)
Frame = +2
Query: 179 VLEQIVNCHDAIAQE---YLMDVVIQV---------FPDEFHLETLET----LLRATSEL 310
++ QI H I QE L ++ ++V E L+ ET +L S
Sbjct: 1024 IINQIQESHAEIIQEKEKQLQELKLKVSDLSDTRCKLEVELALKEAETDEIKILLEESRA 1083
Query: 311 EPNVHIKSIIGALIERLSQYATEESGTGIPDSIPLFDIFSREVNSMVERRKDLPPEDIMS 490
+ +KS++ E L + + I D+ + + E+ +++ KD +++S
Sbjct: 1084 QQKETLKSLLEQETENL-RTEISKLNQKIQDNNENYQVGLAELRTLMTIEKDQCISELIS 1142
Query: 491 LEASLLHLALKVYPNKLELVDNVVKGATEALRNMNISKISYSSCLSRELIRLLKMPIDVY 670
++ LK NK+ + N A E +N+ I S L EL L +
Sbjct: 1143 RHEEESNI-LKAELNKVTSLHNQ---AFEIEKNLKEQIIELQSKLDSELSALERQ----- 1193
Query: 671 KDIRIILKLENYSDLIQNLEYNGRKLIS 754
KD +I + E Y +IQNLE + +KL+S
Sbjct: 1194 KDEKITQQEEKYEAIIQNLEKDRQKLVS 1221
>sp|Q859W7|YCF2_ANTFO Protein ycf2
Length = 2392
Score = 32.3 bits (72), Expect = 1.7
Identities = 16/63 (25%), Positives = 34/63 (53%)
Frame = +3
Query: 351 LNDFLNMQLKKVEQEFLTVFLYSTYFQERLIQWWNAVKICPLKISCLLKHHYFIWH*KFI 530
LN+ + +++ +E ++Y T +++L W+ K + + ++K +Y I+ KF
Sbjct: 779 LNEMFHKKVEAHSEENFETYIYPTNHRKKLKLWYELNKGSYICLDNVVKKYYSIYKLKFY 838
Query: 531 LIN 539
LIN
Sbjct: 839 LIN 841
>sp|O60566|BUB1B_HUMAN Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (hBUBR1)
(MAD3/BUB1-related protein kinase) (Mitotic checkpoint
kinase MAD3L) (SSK1)
Length = 1050
Score = 31.6 bits (70), Expect = 2.8
Identities = 26/94 (27%), Positives = 48/94 (51%), Gaps = 1/94 (1%)
Frame = +3
Query: 282 KLFYEPPQNWSQMYI*NL*LEH*LNDF-LNMQLKKVEQEFLTVFLYSTYFQERLIQWWNA 458
KLF+ P+N +++ + + + DF +N++LK+ E F +Q+ I W
Sbjct: 779 KLFWVAPRNSAELTVIKVSSQPVPWDFYINLKLKERLNEDFDHFCSCYQYQDGCIVWHQY 838
Query: 459 VKICPLKISCLLKHHYFIWH*KFILINWNLSTML 560
+ L+ LL+H +I H +LI +NL T++
Sbjct: 839 INCFTLQD--LLQHSEYITHEITVLIIYNLLTIV 870
>sp|Q61098|IL18R_MOUSE Interleukin-18 receptor 1 precursor (IL1 receptor-related protein)
(IL-1Rrp)
Length = 537
Score = 30.8 bits (68), Expect = 4.8
Identities = 20/71 (28%), Positives = 36/71 (50%), Gaps = 2/71 (2%)
Frame = +2
Query: 518 LKVYPNKLELVDNVVKGATEALRN--MNISKISYSSCLSRELIRLLKMPIDVYKDIRIIL 691
L+ +P ++E + RN +N++K + SC S +L+ ++V K + I
Sbjct: 84 LEFWPVEMEDEGTYISQVGNDRRNWTLNVTKRNKHSCFSDKLVT--SRDVEVNKSLHITC 141
Query: 692 KLENYSDLIQN 724
K NY +LIQ+
Sbjct: 142 KNPNYEELIQD 152
>sp|Q8TMT1|DNLI_METAC DNA ligase (Polydeoxyribonucleotide synthase [ATP])
Length = 568
Score = 30.4 bits (67), Expect = 6.3
Identities = 26/78 (33%), Positives = 37/78 (47%), Gaps = 6/78 (7%)
Frame = +2
Query: 365 QYATEESGTGIPDSIPLFDIFSREVNS-----MVERRKDLPPEDIMSLEAS-LLHLALKV 526
+Y EE GIP + LFDI + +VERRK L S+E S + + +V
Sbjct: 329 KYDVEEKALGIPIQLNLFDIMYLNGRTLIDLPLVERRKALESCVESSVEDSKSISVDEQV 388
Query: 527 YPNKLELVDNVVKGATEA 580
LELV+ + + A A
Sbjct: 389 ITGDLELVEKIYREALNA 406
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86,937,718
Number of Sequences: 369166
Number of extensions: 1668804
Number of successful extensions: 4613
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4483
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4604
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8100769320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)