Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_E20
(767 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q5M8Y1|SPCS2_XENTR Probable signal peptidase complex sub... 157 3e-38
sp|Q28250|SPCS2_CANFA Signal peptidase complex subunit 2 (M... 155 9e-38
sp|Q15005|SPCS2_HUMAN Signal peptidase complex subunit 2 (M... 155 9e-38
sp|Q5RAY6|SPCS2_PONPY Signal peptidase complex subunit 2 (M... 155 9e-38
sp|Q9CYN2|SPCS2_MOUSE Signal peptidase complex subunit 2 (M... 155 9e-38
sp|Q4R512|SPCS2_MACFA Signal peptidase complex subunit 2 (M... 155 9e-38
sp|Q5BJI9|SPCS2_BRARE Probable signal peptidase complex sub... 152 7e-37
sp|Q9VYY2|SPCS2_DROME Probable signal peptidase complex sub... 109 9e-24
sp|Q615A2|SPCS2_CAEBR Probable signal peptidase complex sub... 99 1e-20
sp|Q9XWW1|SPCS2_CAEEL Probable signal peptidase complex sub... 98 2e-20
>sp|Q5M8Y1|SPCS2_XENTR Probable signal peptidase complex subunit 2 (Microsomal signal
peptidase 25 kDa subunit) (SPase 25 kDa subunit)
Length = 201
Score = 157 bits (397), Expect = 3e-38
Identities = 74/170 (43%), Positives = 109/170 (64%)
Frame = +3
Query: 12 QMPEKAIKVDKWDTNATKNALDDGFKKILKEQYKFEEKFALIDGRLLISTVAVLCGGVAV 191
++ +K +K+DKWD +A KN+LDD KK+L E+Y++ E F LIDGRL+I T++ + VA+
Sbjct: 15 KIDDKPVKIDKWDGSAVKNSLDDAAKKVLLEKYRYVENFCLIDGRLIICTISCVFAIVAL 74
Query: 192 LYDWFYPFPASKLVLIVCVLLYFALTGLLTCYIMFVERSTFFLAHENDTAGLDKPIKWNF 371
++D+ +PFP SK VL +CV+ YF + G+LT Y + E+S F +AH D AG+D W+
Sbjct: 75 VWDYLHPFPESKPVLAICVISYFLMMGILTIYTSYKEKSIFLVAHRKDPAGMDPDDIWHL 134
Query: 372 QSKMKKYQDKYELTIKREDLNAKQSNLMTESKSIAEFFDIKGNLRMDILE 521
S +K++ DKY L + K +KSIA FFD G L MD+ E
Sbjct: 135 SSSLKRFDDKYTLKVTYISGKTKAQRDAEFTKSIARFFDDNGTLVMDLFE 184
>sp|Q28250|SPCS2_CANFA Signal peptidase complex subunit 2 (Microsomal signal peptidase 25
kDa subunit) (SPase 25 kDa subunit)
Length = 226
Score = 155 bits (393), Expect = 9e-38
Identities = 75/170 (44%), Positives = 106/170 (62%)
Frame = +3
Query: 12 QMPEKAIKVDKWDTNATKNALDDGFKKILKEQYKFEEKFALIDGRLLISTVAVLCGGVAV 191
++ +K +K+DKWD +A KN+LDD KK+L E+YK+ E F LIDGRL I T++ VA+
Sbjct: 40 KIDDKPVKIDKWDGSAVKNSLDDSAKKVLLEKYKYVENFGLIDGRLTICTISCFFAIVAL 99
Query: 192 LYDWFYPFPASKLVLIVCVLLYFALTGLLTCYIMFVERSTFFLAHENDTAGLDKPIKWNF 371
++D+ +PFP SK VL +CV+ YF + G+LT Y + E+S F +AH D G+D W
Sbjct: 100 IWDYMHPFPESKPVLALCVISYFVMMGILTIYTSYKEKSIFLVAHRKDPTGMDPDDIWQL 159
Query: 372 QSKMKKYQDKYELTIKREDLNAKQSNLMTESKSIAEFFDIKGNLRMDILE 521
S +K++ DKY L + KQ +KSIA+FFD G L MD E
Sbjct: 160 SSSLKRFDDKYTLKLTFISGRTKQQREAEFTKSIAKFFDHSGTLVMDAYE 209
>sp|Q15005|SPCS2_HUMAN Signal peptidase complex subunit 2 (Microsomal signal peptidase 25
kDa subunit) (SPase 25 kDa subunit)
Length = 226
Score = 155 bits (393), Expect = 9e-38
Identities = 75/170 (44%), Positives = 106/170 (62%)
Frame = +3
Query: 12 QMPEKAIKVDKWDTNATKNALDDGFKKILKEQYKFEEKFALIDGRLLISTVAVLCGGVAV 191
++ +K +K+DKWD +A KN+LDD KK+L E+YK+ E F LIDGRL I T++ VA+
Sbjct: 40 KIDDKPVKIDKWDGSAVKNSLDDSAKKVLLEKYKYVENFGLIDGRLTICTISCFFAIVAL 99
Query: 192 LYDWFYPFPASKLVLIVCVLLYFALTGLLTCYIMFVERSTFFLAHENDTAGLDKPIKWNF 371
++D+ +PFP SK VL +CV+ YF + G+LT Y + E+S F +AH D G+D W
Sbjct: 100 IWDYMHPFPESKPVLALCVISYFVMMGILTIYTSYKEKSIFLVAHRKDPTGMDPDDIWQL 159
Query: 372 QSKMKKYQDKYELTIKREDLNAKQSNLMTESKSIAEFFDIKGNLRMDILE 521
S +K++ DKY L + KQ +KSIA+FFD G L MD E
Sbjct: 160 SSSLKRFDDKYTLKLTFISGRTKQQREAEFTKSIAKFFDHSGTLVMDAYE 209
>sp|Q5RAY6|SPCS2_PONPY Signal peptidase complex subunit 2 (Microsomal signal peptidase 25
kDa subunit) (SPase 25 kDa subunit)
Length = 226
Score = 155 bits (393), Expect = 9e-38
Identities = 75/170 (44%), Positives = 106/170 (62%)
Frame = +3
Query: 12 QMPEKAIKVDKWDTNATKNALDDGFKKILKEQYKFEEKFALIDGRLLISTVAVLCGGVAV 191
++ +K +K+DKWD +A KN+LDD KK+L E+YK+ E F LIDGRL I T++ VA+
Sbjct: 40 KIDDKPVKIDKWDGSAVKNSLDDSAKKVLLEKYKYVENFGLIDGRLTICTISCFFAIVAL 99
Query: 192 LYDWFYPFPASKLVLIVCVLLYFALTGLLTCYIMFVERSTFFLAHENDTAGLDKPIKWNF 371
++D+ +PFP SK VL +CV+ YF + G+LT Y + E+S F +AH D G+D W
Sbjct: 100 IWDYMHPFPESKPVLALCVISYFVMMGILTIYTSYKEKSIFLVAHRKDPTGMDPDDIWQL 159
Query: 372 QSKMKKYQDKYELTIKREDLNAKQSNLMTESKSIAEFFDIKGNLRMDILE 521
S +K++ DKY L + KQ +KSIA+FFD G L MD E
Sbjct: 160 SSSLKRFDDKYTLKLTFISGRTKQQREAEFTKSIAKFFDHSGTLVMDAYE 209
>sp|Q9CYN2|SPCS2_MOUSE Signal peptidase complex subunit 2 (Microsomal signal peptidase 25
kDa subunit) (SPase 25 kDa subunit)
Length = 226
Score = 155 bits (393), Expect = 9e-38
Identities = 75/170 (44%), Positives = 106/170 (62%)
Frame = +3
Query: 12 QMPEKAIKVDKWDTNATKNALDDGFKKILKEQYKFEEKFALIDGRLLISTVAVLCGGVAV 191
++ +K +K+DKWD +A KN+LDD KK+L E+YK+ E F LIDGRL I T++ VA+
Sbjct: 40 KIDDKPVKIDKWDGSAVKNSLDDSAKKVLLEKYKYVENFGLIDGRLTICTISCFFAIVAL 99
Query: 192 LYDWFYPFPASKLVLIVCVLLYFALTGLLTCYIMFVERSTFFLAHENDTAGLDKPIKWNF 371
++D+ +PFP SK VL +CV+ YF + G+LT Y + E+S F +AH D G+D W
Sbjct: 100 IWDYMHPFPESKPVLALCVISYFVMMGILTIYTSYKEKSIFLVAHRKDPTGMDPDDIWQL 159
Query: 372 QSKMKKYQDKYELTIKREDLNAKQSNLMTESKSIAEFFDIKGNLRMDILE 521
S +K++ DKY L + KQ +KSIA+FFD G L MD E
Sbjct: 160 SSSLKRFDDKYTLKLTFISGRTKQQREAEFTKSIAKFFDHSGTLVMDAYE 209
>sp|Q4R512|SPCS2_MACFA Signal peptidase complex subunit 2 (Microsomal signal peptidase 25
kDa subunit) (SPase 25 kDa subunit)
Length = 226
Score = 155 bits (393), Expect = 9e-38
Identities = 75/170 (44%), Positives = 106/170 (62%)
Frame = +3
Query: 12 QMPEKAIKVDKWDTNATKNALDDGFKKILKEQYKFEEKFALIDGRLLISTVAVLCGGVAV 191
++ +K +K+DKWD +A KN+LDD KK+L E+YK+ E F LIDGRL I T++ VA+
Sbjct: 40 KIDDKPVKIDKWDGSAVKNSLDDSAKKVLLEKYKYVENFGLIDGRLTICTISCFFAIVAL 99
Query: 192 LYDWFYPFPASKLVLIVCVLLYFALTGLLTCYIMFVERSTFFLAHENDTAGLDKPIKWNF 371
++D+ +PFP SK VL +CV+ YF + G+LT Y + E+S F +AH D G+D W
Sbjct: 100 IWDYMHPFPESKPVLALCVISYFVMMGILTIYTSYKEKSIFLVAHRKDPTGMDPDDIWQL 159
Query: 372 QSKMKKYQDKYELTIKREDLNAKQSNLMTESKSIAEFFDIKGNLRMDILE 521
S +K++ DKY L + KQ +KSIA+FFD G L MD E
Sbjct: 160 SSSLKRFDDKYTLKLTFISGRTKQQREAEFTKSIAKFFDHSGTLVMDAYE 209
>sp|Q5BJI9|SPCS2_BRARE Probable signal peptidase complex subunit 2 (Microsomal signal
peptidase 25 kDa subunit) (SPase 25 kDa subunit)
Length = 201
Score = 152 bits (385), Expect = 7e-37
Identities = 76/172 (44%), Positives = 108/172 (62%)
Frame = +3
Query: 12 QMPEKAIKVDKWDTNATKNALDDGFKKILKEQYKFEEKFALIDGRLLISTVAVLCGGVAV 191
++ EK +K+DKWD A KN+LDD KK+L E+Y + E F L+DGRL I TV+ L VA+
Sbjct: 15 RIDEKPVKIDKWDGAAVKNSLDDAAKKVLIEKYGYLESFNLVDGRLFICTVSCLFTIVAL 74
Query: 192 LYDWFYPFPASKLVLIVCVLLYFALTGLLTCYIMFVERSTFFLAHENDTAGLDKPIKWNF 371
++D+ +PFP SK VL CV+ YF + G+LT Y + E++ F +A + D AG+D W
Sbjct: 75 IWDYLHPFPESKPVLACCVVSYFIMMGILTLYTSYKEKNIFLVAMQKDPAGMDPDHSWCL 134
Query: 372 QSKMKKYQDKYELTIKREDLNAKQSNLMTESKSIAEFFDIKGNLRMDILEKY 527
S +K++ D+Y L + D KQS +KS++ FFD G L MD EKY
Sbjct: 135 SSSLKRFDDQYTLRMSFTDGKTKQSRETEFTKSVSVFFDENGTLVMDQYEKY 186
>sp|Q9VYY2|SPCS2_DROME Probable signal peptidase complex subunit 2 (Microsomal signal
peptidase 25 kDa subunit) (SPase 25 kDa subunit)
Length = 199
Score = 109 bits (272), Expect = 9e-24
Identities = 62/163 (38%), Positives = 95/163 (58%), Gaps = 1/163 (0%)
Frame = +3
Query: 12 QMPEKAIKVDKWDTNATKNALDDGFKK-ILKEQYKFEEKFALIDGRLLISTVAVLCGGVA 188
Q E+ +KV+KWD +A K+ALDD K +L ++ + +E+F L++ RL + +AV +A
Sbjct: 9 QQGEELVKVNKWDGSAVKHALDDAVKTCLLGDRPQLKEQFGLVNTRLALCALAVSVAIMA 68
Query: 189 VLYDWFYPFPASKLVLIVCVLLYFALTGLLTCYIMFVERSTFFLAHENDTAGLDKPIKWN 368
+D+ +PFP S+ VL+ VL YFAL G+LT + F E+ TF +A + D ++ W
Sbjct: 69 HAWDFTHPFPESRPVLLFSVLAYFALLGILTLHSSFREKGTFAVALQKDK---ERERLWE 125
Query: 369 FQSKMKKYQDKYELTIKREDLNAKQSNLMTESKSIAEFFDIKG 497
S M+KY DKY LT+ D + + +KS A F D G
Sbjct: 126 ASSDMRKYDDKYLLTLSVRDTKNGKRREQSSNKSCAAFIDQNG 168
>sp|Q615A2|SPCS2_CAEBR Probable signal peptidase complex subunit 2 (Microsomal signal
peptidase 25 kDa subunit) (SPase 25 kDa subunit)
Length = 180
Score = 99.0 bits (245), Expect = 1e-20
Identities = 57/169 (33%), Positives = 98/169 (57%), Gaps = 1/169 (0%)
Frame = +3
Query: 21 EKAIKVDKWDTNATKNALDDGFKKILKEQYKFEEKFALIDGRLLISTVAVLCGGVAVLYD 200
E+ V+KWD KN LD+ KKIL ++ + E+ L++ RLLIS + V A YD
Sbjct: 4 ERITVVNKWDGPTVKNGLDEVVKKILNDKVGWTEQHNLMNLRLLISFIGVAFSAFACGYD 63
Query: 201 WFYPFPASKLVLIVCVLLYFALTGLLTCYIMFVERSTFFLAHENDTAGLDKPIKWNFQSK 380
++ PFP SK+VL+VC + YF G+L + +VE+ F+ A+E D + KW + S+
Sbjct: 64 FYAPFPKSKIVLLVCSVSYFICMGVLQLFQWYVEKDCFYEANEVDG---KQTRKWAWSSE 120
Query: 381 MKKYQDKYELTIK-REDLNAKQSNLMTESKSIAEFFDIKGNLRMDILEK 524
+K + DKY L+ + +++ + Q ++ KSI + D G + + ++++
Sbjct: 121 IKAHDDKYVLSAEFKKEGRSGQGKII---KSIGAYIDNDGEIMIPLVQR 166
>sp|Q9XWW1|SPCS2_CAEEL Probable signal peptidase complex subunit 2 (Microsomal signal
peptidase 25 kDa subunit) (SPase 25 kDa subunit)
Length = 180
Score = 98.2 bits (243), Expect = 2e-20
Identities = 61/171 (35%), Positives = 94/171 (54%), Gaps = 1/171 (0%)
Frame = +3
Query: 15 MPEKAIKV-DKWDTNATKNALDDGFKKILKEQYKFEEKFALIDGRLLISTVAVLCGGVAV 191
M ++ +KV +KWD KNALD+ KKIL ++ + E L++ RLLIS + V A
Sbjct: 1 MTDEPVKVVNKWDGPTVKNALDEVVKKILNDKVGWTESHNLMNLRLLISFIGVAFSAFAC 60
Query: 192 LYDWFYPFPASKLVLIVCVLLYFALTGLLTCYIMFVERSTFFLAHENDTAGLDKPIKWNF 371
YD++ PFP SK+VL VC + YF G+L Y +VE+ + A E D + KW +
Sbjct: 61 GYDYYEPFPKSKIVLAVCSVSYFICMGILQMYQWYVEKDCIYEATEVDG---KQSRKWAW 117
Query: 372 QSKMKKYQDKYELTIKREDLNAKQSNLMTESKSIAEFFDIKGNLRMDILEK 524
S++K + DKY T+ E +S +KSI + D G + + +++K
Sbjct: 118 SSEIKAHDDKY--TLSAEFKKEGRSGQGKITKSIGAYIDNDGEIIVPLVKK 166
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 78,507,303
Number of Sequences: 369166
Number of extensions: 1428474
Number of successful extensions: 4346
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4329
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7115329200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)