Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_E13
(761 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P28648|CD63_RAT CD63 antigen (AD1 antigen) 53 8e-07
sp|P41731|CD63_MOUSE CD63 antigen 51 4e-06
sp|P08962|CD63_HUMAN CD63 antigen (Melanoma-associated anti... 44 5e-04
sp|O60637|TSN3_HUMAN Tetraspanin-3 (Tspan-3) (Transmembrane... 44 6e-04
sp|P40237|CD82_MOUSE CD82 antigen (Inducible membrane prote... 43 0.001
sp|Q9QY33|TSN3_MOUSE Tetraspanin-3 (Tspan-3) (Transmembrane... 42 0.001
sp|Q80WR1|TSN18_MOUSE Tetraspanin-18 (Tspan-18) 42 0.002
sp|O43657|TSN6_HUMAN Tetraspanin-6 (Tspan-6) (Transmembrane... 42 0.002
sp|Q96SJ8|TSN18_HUMAN Tetraspanin-18 (Tspan-18) 42 0.002
sp|Q58CY8|TSN18_BOVIN Tetraspanin-18 (Tspan-18) 41 0.003
>sp|P28648|CD63_RAT CD63 antigen (AD1 antigen)
Length = 238
Score = 53.1 bits (126), Expect = 8e-07
Identities = 46/193 (23%), Positives = 74/193 (38%), Gaps = 4/193 (2%)
Frame = +3
Query: 141 AIKENKCLLITY----SILXXXXXXXXXXXXXXXXXEKNKFQKNLGKSLTNVLEKDFNGI 308
A KEN CL+IT+ S++ K++F K+ K + N L
Sbjct: 75 ACKENYCLMITFAIFLSLIMLVEVAVAIAGYVFRDQVKSEFSKSFQKQMQNYLTD----- 129
Query: 309 LNTNATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWKSTSSSGTKQIYPVGCCKLKEKYQ 488
N AT+ K+ E KCCG +N TD++ + K P CC
Sbjct: 130 -NKTATILDKL----QKENKCCGASNYTDWERIPGM-------AKDRVPDSCC------- 170
Query: 489 YDPNKKFEYNDAKKNFENDNCPFDNDKSTININGCIPSLIDLIEKNMXXXXXXXXXXXXF 668
N C D +STI+ GC+ ++ + KN+
Sbjct: 171 -------------INI-TVGCGNDFKESTIHTQGCVETIAAWLRKNVLLVAGAALGIAFV 216
Query: 669 EIMCIVFACCVMR 707
E++ I+F+CC+++
Sbjct: 217 EVLGIIFSCCLVK 229
>sp|P41731|CD63_MOUSE CD63 antigen
Length = 238
Score = 50.8 bits (120), Expect = 4e-06
Identities = 44/193 (22%), Positives = 75/193 (38%), Gaps = 4/193 (2%)
Frame = +3
Query: 141 AIKENKCLLITY----SILXXXXXXXXXXXXXXXXXEKNKFQKNLGKSLTNVLEKDFNGI 308
A KEN CL+IT+ S++ K++F K+ + + N L+
Sbjct: 75 ACKENYCLMITFAIFLSLIMLVEVAVAIAGYVFRDQVKSEFNKSFQQQMQNYLKD----- 129
Query: 309 LNTNATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWKSTSSSGTKQIYPVGCCKLKEKYQ 488
N AT+ K+ E CCG +N TD++++ K P CC
Sbjct: 130 -NKTATILDKL----QKENNCCGASNYTDWENIPGM-------AKDRVPDSCC------- 170
Query: 489 YDPNKKFEYNDAKKNFENDNCPFDNDKSTININGCIPSLIDLIEKNMXXXXXXXXXXXXF 668
N C D +STI+ GC+ ++ + KN+
Sbjct: 171 -------------INI-TVGCGNDFKESTIHTQGCVETIAIWLRKNILLVAAAALGIAFV 216
Query: 669 EIMCIVFACCVMR 707
E++ I+F+CC+++
Sbjct: 217 EVLGIIFSCCLVK 229
>sp|P08962|CD63_HUMAN CD63 antigen (Melanoma-associated antigen ME491)
(Lysosome-associated membrane glycoprotein 3) (LAMP-3)
(Ocular melanoma-associated antigen) (OMA81H)
(Granulophysin) (Tetraspanin-30) (Tspan-30)
Length = 238
Score = 43.9 bits (102), Expect = 5e-04
Identities = 39/189 (20%), Positives = 65/189 (34%)
Frame = +3
Query: 141 AIKENKCLLITYSILXXXXXXXXXXXXXXXXXEKNKFQKNLGKSLTNVLEKDFNGILNTN 320
A KEN CL+IT++I ++K + +E N
Sbjct: 75 ACKENYCLMITFAIFLSLIMLVEVAAAIAGYVFRDKVMSEFNNNFRQQMEN----YPKNN 130
Query: 321 ATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWKSTSSSGTKQIYPVGCCKLKEKYQYDPN 500
T I + + KCCG N TD++ + S +K P CC
Sbjct: 131 HT--ASILDRMQADFKCCGAANYTDWEKI-------PSMSKNRVPDSCCINVTV------ 175
Query: 501 KKFEYNDAKKNFENDNCPFDNDKSTININGCIPSLIDLIEKNMXXXXXXXXXXXXFEIMC 680
C + ++ I+ GC+ + + KN+ E++
Sbjct: 176 ---------------GCGINFNEKAIHKEGCVEKIGGWLRKNVLVVAAAALGIAFVEVLG 220
Query: 681 IVFACCVMR 707
IVFACC+++
Sbjct: 221 IVFACCLVK 229
>sp|O60637|TSN3_HUMAN Tetraspanin-3 (Tspan-3) (Transmembrane 4 superfamily member 8)
(Tetraspanin TM4-A)
Length = 253
Score = 43.5 bits (101), Expect = 6e-04
Identities = 29/133 (21%), Positives = 57/133 (42%)
Frame = +3
Query: 72 VVAIVLIIXXXXXXXXXXXXXXXAIKENKCLLITYSILXXXXXXXXXXXXXXXXXEKNKF 251
+ A+V+I I+E++C L T+ I+ + K
Sbjct: 52 IPAVVIIAVGALLFIIGLIGCCATIRESRCGLATFVIILLLVFVTEVVVVVLGYVYRAKV 111
Query: 252 QKNLGKSLTNVLEKDFNGILNTNATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWKSTSS 431
+ + +S+ V K +NG TN ++ + +L CCG++N +D+++ +K T +
Sbjct: 112 ENEVDRSIQKVY-KTYNG---TNPDAASRAIDYVQRQLHCCGIHNYSDWENTDWFKETKN 167
Query: 432 SGTKQIYPVGCCK 470
Q P+ CC+
Sbjct: 168 ----QSVPLSCCR 176
>sp|P40237|CD82_MOUSE CD82 antigen (Inducible membrane protein R2) (C33 antigen) (IA4)
Length = 266
Score = 42.7 bits (99), Expect = 0.001
Identities = 47/222 (21%), Positives = 83/222 (37%), Gaps = 3/222 (1%)
Frame = +3
Query: 51 TNSTILDVVAIVLIIXXXXXXXXXXXXXXXAIKENKCLLITYSILXXXXXXXXXXXXXXX 230
T+S+ L V A V I A+ E +CLL Y +
Sbjct: 47 TSSSSLQVGAYVFIGVGAITIVMGFLGCIGAVNEVRCLLGLYFVFLLLILIAQVTVGVLF 106
Query: 231 XXEKNKFQKNLGKSLTNVLEKDFNGILNTNATLFTK-IFTAIMFELKCCGVNNVTDFDSV 407
+K +K +G ++ +++ NAT + + + ++KCCG + S
Sbjct: 107 YFNADKLKKEMGNTVMDIIRN-----YTANATSSREEAWDYVQAQVKCCG------WVSH 155
Query: 408 RNWKSTSS--SGTKQIYPVGCCKLKEKYQYDPNKKFEYNDAKKNFENDNCPFDNDKSTIN 581
NW TK YP C K+KE+ KK + +A + ++N P D +N
Sbjct: 156 YNWTENEELMGFTKTTYPCSCEKIKEEDNQLIVKK-GFCEADNSTVSENNPED---WPVN 211
Query: 582 INGCIPSLIDLIEKNMXXXXXXXXXXXXFEIMCIVFACCVMR 707
GC+ +++N E++ + + C+ R
Sbjct: 212 TEGCMEKAQAWLQENFGILLGVCAGVAVIELLGLFLSICLCR 253
>sp|Q9QY33|TSN3_MOUSE Tetraspanin-3 (Tspan-3) (Transmembrane 4 superfamily member 8)
(Tetraspanin TM4-A) (OSP-associated protein 1) (OAP-1)
Length = 253
Score = 42.4 bits (98), Expect = 0.001
Identities = 28/131 (21%), Positives = 56/131 (42%)
Frame = +3
Query: 78 AIVLIIXXXXXXXXXXXXXXXAIKENKCLLITYSILXXXXXXXXXXXXXXXXXEKNKFQK 257
A+V+I I+E++C L T+ + + K +
Sbjct: 54 AVVIIAVGALLFIIGLIGCCATIRESRCGLATFVFILLLVFVTEVVVVVLGYVYRAKVEN 113
Query: 258 NLGKSLTNVLEKDFNGILNTNATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWKSTSSSG 437
+ +S+ V K +NG TN+ ++ + +L CCG++N +D+++ +K T +
Sbjct: 114 EVDRSIQKVY-KTYNG---TNSDAASRAIDYVQRQLHCCGIHNYSDWENTDWFKETKN-- 167
Query: 438 TKQIYPVGCCK 470
Q P+ CC+
Sbjct: 168 --QSVPLSCCR 176
>sp|Q80WR1|TSN18_MOUSE Tetraspanin-18 (Tspan-18)
Length = 248
Score = 42.0 bits (97), Expect = 0.002
Identities = 40/190 (21%), Positives = 78/190 (41%), Gaps = 1/190 (0%)
Frame = +3
Query: 141 AIKENKCLLITYSILXXXXXXXXXXXXXXXXXEKNKFQKNLGKSL-TNVLEKDFNGILNT 317
A++EN+CLL+ + + F+++L + T L K + G +T
Sbjct: 76 AVRENRCLLLFFFLFILIIFLVELSAAILAFI----FREHLTREFFTKELTKHYQGDNDT 131
Query: 318 NATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWKSTSSSGTKQIYPVGCCKLKEKYQYDP 497
+ +F+ + ++M CCGVN DF ++ + T+++ P CC+ +P
Sbjct: 132 D--VFSATWNSVMITFGCCGVNGPEDFKLASVFRLLTLD-TEEV-PKACCR------REP 181
Query: 498 NKKFEYNDAKKNFENDNCPFDNDKSTININGCIPSLIDLIEKNMXXXXXXXXXXXXFEIM 677
+ +++ + PF IN GC +++ E + E+
Sbjct: 182 QTRDGVVLSREECQLGRNPF------INKQGCYTVILNTFETYVYLAGAFAIGVLAIELF 235
Query: 678 CIVFACCVMR 707
+VFA C+ R
Sbjct: 236 LMVFAMCLFR 245
>sp|O43657|TSN6_HUMAN Tetraspanin-6 (Tspan-6) (Transmembrane 4 superfamily member 6)
(T245 protein) (Tetraspanin TM4-D) (A15 homolog)
Length = 245
Score = 42.0 bits (97), Expect = 0.002
Identities = 39/156 (25%), Positives = 58/156 (37%)
Frame = +3
Query: 240 KNKFQKNLGKSLTNVLEKDFNGILNTNATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWK 419
KN F+ N K+L K +N + + KI L CCGV TD+ R+W
Sbjct: 119 KNSFKNNYEKAL-----KQYNSTGDYRSHAVDKIQNT----LHCCGV---TDY---RDWT 163
Query: 420 STSSSGTKQIYPVGCCKLKEKYQYDPNKKFEYNDAKKNFENDNCPFDNDKSTININGCIP 599
T+ K +P CCKL++ C D +N GC
Sbjct: 164 DTNYYSEKG-FPKSCCKLED-----------------------CTPQRDADKVNNEGCFI 199
Query: 600 SLIDLIEKNMXXXXXXXXXXXXFEIMCIVFACCVMR 707
++ +IE M F+++ I A C+ R
Sbjct: 200 KVMTIIESEMGVVAGISFGVACFQLIGIFLAYCLSR 235
>sp|Q96SJ8|TSN18_HUMAN Tetraspanin-18 (Tspan-18)
Length = 248
Score = 41.6 bits (96), Expect = 0.002
Identities = 39/190 (20%), Positives = 70/190 (36%), Gaps = 1/190 (0%)
Frame = +3
Query: 141 AIKENKCLLITYSILXXXXXXXXXXXXXXXXXEKNKFQKNLGKSL-TNVLEKDFNGILNT 317
A++ENKCLL+ + + F++NL + T L K + G N
Sbjct: 76 AVRENKCLLLFFFLFILIIFLAELSAAILAFI----FRENLTREFFTKELTKHYQG--NN 129
Query: 318 NATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWKSTSSSGTKQIYPVGCCKLKEKYQYDP 497
+ +F+ + ++M CCGVN DF ++ + + P CC+ +E D
Sbjct: 130 DTDVFSATWNSVMITFGCCGVNGPEDFKFASVFRLLTLDSEE--VPEACCR-REPQSRD- 185
Query: 498 NKKFEYNDAKKNFENDNCPFDNDKSTININGCIPSLIDLIEKNMXXXXXXXXXXXXFEIM 677
+ C +N GC +++ E + E+
Sbjct: 186 ---------GVLLSREECLLGR-SLFLNKQGCYTVILNTFETYVYLAGALAIGVLAIELF 235
Query: 678 CIVFACCVMR 707
++FA C+ R
Sbjct: 236 AMIFAMCLFR 245
>sp|Q58CY8|TSN18_BOVIN Tetraspanin-18 (Tspan-18)
Length = 249
Score = 41.2 bits (95), Expect = 0.003
Identities = 40/190 (21%), Positives = 71/190 (37%), Gaps = 1/190 (0%)
Frame = +3
Query: 141 AIKENKCLLITYSILXXXXXXXXXXXXXXXXXEKNKFQKNLGKSL-TNVLEKDFNGILNT 317
A++ENKCLL+ + + F+ NL + T L K + G +T
Sbjct: 76 AVRENKCLLLFFFLFILIIFLAELSAAILAFI----FRGNLTREFFTKELTKHYQGSNDT 131
Query: 318 NATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWKSTSSSGTKQIYPVGCCKLKEKYQYDP 497
+ +F+ + ++M CCGVN DF ++ + + P CC+ +E D
Sbjct: 132 D--VFSATWNSVMITFGCCGVNGPEDFKYASVFRLLTLDSDE--VPEACCR-REPQSRD- 185
Query: 498 NKKFEYNDAKKNFENDNCPFDNDKSTININGCIPSLIDLIEKNMXXXXXXXXXXXXFEIM 677
+ C D +N GC +++ E + E+
Sbjct: 186 ---------GVLLSREECLLGRD-LFLNKQGCYTVILNAFETYVYLAGALAIGVLAIELF 235
Query: 678 CIVFACCVMR 707
++FA C+ R
Sbjct: 236 AMIFAMCLFR 245
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 78,128,487
Number of Sequences: 369166
Number of extensions: 1519618
Number of successful extensions: 3842
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3683
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3818
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7018522000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)