Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_E12
(712 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P56377|AP1S2_HUMAN Adapter-related protein complex 1 sig... 235 1e-61
sp|Q9DB50|AP1S2_MOUSE Adapter-related protein complex 1 sig... 235 1e-61
sp|P61967|AP1S1_MOUSE Adapter-related protein complex 1 sig... 232 6e-61
sp|Q96PC3|AP1S3_HUMAN Adapter-related protein complex 1 sig... 211 2e-54
sp|P35181|AP19_YEAST Clathrin coat assembly protein AP19 (C... 156 6e-38
sp|P62743|AP2S1_MOUSE Clathrin coat assembly protein AP17 (... 138 1e-32
sp|O50016|A2S1_MAIZE Clathrin coat assembly protein AP17 (C... 132 7e-31
sp|Q9WVL1|AP4S1_MOUSE Adapter-related protein complex 4 sig... 113 6e-25
sp|Q9Y587|AP4S1_HUMAN Adapter-related protein complex 4 sig... 113 6e-25
sp|Q00381|AP17_YEAST Clathrin coat assembly protein AP17 (C... 110 4e-24
>sp|P56377|AP1S2_HUMAN Adapter-related protein complex 1 sigma 1B subunit (Sigma-adaptin
1B) (Adaptor protein complex AP-1 sigma-1B subunit)
(Golgi adaptor HA1/AP1 adaptin sigma-1B subunit)
(Clathrin assembly protein complex 1 sigma-1B small
chain) (Sigma 1B subunit of AP-1 clathrin)
Length = 157
Score = 235 bits (599), Expect = 1e-61
Identities = 109/139 (78%), Positives = 125/139 (89%)
Frame = +2
Query: 2 FSRQGKVRLQKWYVAHSDKNRKKILRELTSLVLTRKPKMCAFIEWKDLKIVYRRYASLYF 181
FSRQGK+RLQKWYV SDK +KKI REL VL RKPKMC+F+EW+DLKIVY+RYASLYF
Sbjct: 7 FSRQGKLRLQKWYVPLSDKEKKKITRELVQTVLARKPKMCSFLEWRDLKIVYKRYASLYF 66
Query: 182 CCAVEREDNELVTLEIIHRYVELLDKYFGSVCELDIIFNFEKAYFMLDEFMLAGEVQETS 361
CCA+E +DNEL+TLEIIHRYVELLDKYFGSVCELDIIFNFEKAYF+LDEF+L GEVQETS
Sbjct: 67 CCAIEDQDNELITLEIIHRYVELLDKYFGSVCELDIIFNFEKAYFILDEFLLGGEVQETS 126
Query: 362 KKNVLKAVDAQDMIQENED 418
KKNVLKA++ D++QE +
Sbjct: 127 KKNVLKAIEQADLLQEEAE 145
>sp|Q9DB50|AP1S2_MOUSE Adapter-related protein complex 1 sigma 1B subunit (Sigma-adaptin
1B) (Adaptor protein complex AP-1 sigma-1B subunit)
(Golgi adaptor HA1/AP1 adaptin sigma-1B subunit)
(Clathrin assembly protein complex 1 sigma-1B small
chain) (Sigma 1B subunit of AP-1 clathrin)
Length = 160
Score = 235 bits (599), Expect = 1e-61
Identities = 109/137 (79%), Positives = 125/137 (91%)
Frame = +2
Query: 2 FSRQGKVRLQKWYVAHSDKNRKKILRELTSLVLTRKPKMCAFIEWKDLKIVYRRYASLYF 181
FSRQGK+RLQKWYV SDK +KKI REL VL RKPKMC+F+EW+DLKIVY+RYASLYF
Sbjct: 7 FSRQGKLRLQKWYVPLSDKEKKKITRELVQTVLARKPKMCSFLEWRDLKIVYKRYASLYF 66
Query: 182 CCAVEREDNELVTLEIIHRYVELLDKYFGSVCELDIIFNFEKAYFMLDEFMLAGEVQETS 361
CCA+E +DNEL+TLEIIHRYVELLDKYFGSVCELDIIFNFEKAYF+LDEF+L GEVQETS
Sbjct: 67 CCAIEDQDNELITLEIIHRYVELLDKYFGSVCELDIIFNFEKAYFILDEFLLGGEVQETS 126
Query: 362 KKNVLKAVDAQDMIQEN 412
KKNVLKA++ D++QE+
Sbjct: 127 KKNVLKAIEQADLLQED 143
>sp|P61967|AP1S1_MOUSE Adapter-related protein complex 1 sigma 1A subunit (Sigma-adaptin
1A) (Adaptor protein complex AP-1 sigma-1A subunit)
(Golgi adaptor HA1/AP1 adaptin sigma-1A subunit)
(Clathrin assembly protein complex 1 sigma-1A small
chain) (Clathrin coat assembly protein AP19) (HA1 19 kDa
subunit) (Sigma 1a subunit of AP-1 clathrin)
sp|P61966|AP1S1_HUMAN Adapter-related protein complex 1 sigma 1A subunit (Sigma-adaptin
1A) (Adaptor protein complex AP-1 sigma-1A subunit)
(Golgi adaptor HA1/AP1 adaptin sigma-1A subunit)
(Clathrin assembly protein complex 1 sigma-1A small
chain) (Clathrin coat assembly protein AP19) (HA1 19 kDa
subunit) (Sigma 1a subunit of AP-1 clathrin)
Length = 158
Score = 232 bits (592), Expect = 6e-61
Identities = 103/139 (74%), Positives = 129/139 (92%)
Frame = +2
Query: 2 FSRQGKVRLQKWYVAHSDKNRKKILRELTSLVLTRKPKMCAFIEWKDLKIVYRRYASLYF 181
FSRQGK+RLQKWY+A SDK RKK++REL +VL RKPKMC+F+EW+DLK+VY+RYASLYF
Sbjct: 8 FSRQGKLRLQKWYLATSDKERKKMVRELMQVVLARKPKMCSFLEWRDLKVVYKRYASLYF 67
Query: 182 CCAVEREDNELVTLEIIHRYVELLDKYFGSVCELDIIFNFEKAYFMLDEFMLAGEVQETS 361
CCA+E +DNEL+TLE+IHRYVELLDKYFGSVCELDIIFNFEKAYF+LDEF++ G+VQ+TS
Sbjct: 68 CCAIEGQDNELITLELIHRYVELLDKYFGSVCELDIIFNFEKAYFILDEFLMGGDVQDTS 127
Query: 362 KKNVLKAVDAQDMIQENED 418
KK+VLKA++ D++QE ++
Sbjct: 128 KKSVLKAIEQADLLQEEDE 146
>sp|Q96PC3|AP1S3_HUMAN Adapter-related protein complex 1 sigma 1C subunit (Sigma-adaptin
1C) (Adaptor protein complex AP-1 sigma-1C subunit)
(Golgi adaptor HA1/AP1 adaptin sigma-1C subunit)
(Clathrin assembly protein complex 1 sigma-1C small
chain) (Sigma 1C subunit of AP-1 clathrin)
Length = 154
Score = 211 bits (536), Expect = 2e-54
Identities = 93/136 (68%), Positives = 119/136 (87%)
Frame = +2
Query: 2 FSRQGKVRLQKWYVAHSDKNRKKILRELTSLVLTRKPKMCAFIEWKDLKIVYRRYASLYF 181
FSRQGK+RLQKWY+ DK RKKI RE+ ++L+R + +F++WK+LK+VY+RYASLYF
Sbjct: 8 FSRQGKLRLQKWYITLPDKERKKITREIVQIILSRGHRTSSFVDWKELKLVYKRYASLYF 67
Query: 182 CCAVEREDNELVTLEIIHRYVELLDKYFGSVCELDIIFNFEKAYFMLDEFMLAGEVQETS 361
CCA+E +DNEL+TLEI+HRYVELLDKYFG+VCELDIIFNFEKAYF+LDEF++ GE+QETS
Sbjct: 68 CCAIENQDNELLTLEIVHRYVELLDKYFGNVCELDIIFNFEKAYFILDEFIIGGEIQETS 127
Query: 362 KKNVLKAVDAQDMIQE 409
KK +KA++ DM+QE
Sbjct: 128 KKIAVKAIEDSDMLQE 143
>sp|P35181|AP19_YEAST Clathrin coat assembly protein AP19 (Clathrin coat-associated
protein AP19) (Golgi adaptor AP-1 19 kDa adaptin) (HA1
19 kDa subunit) (Clathrin assembly protein complex 1
small chain)
Length = 156
Score = 156 bits (394), Expect = 6e-38
Identities = 76/140 (54%), Positives = 103/140 (73%), Gaps = 2/140 (1%)
Frame = +2
Query: 5 SRQGKVRLQKWYVAHSDKNRKKILRELTSLVLTRKPKMCAFIEWKDLKIVYRRYASLYFC 184
SRQGK+RL+KWY A S + KI+++LT +L RKPKMC IE+ D K+VY+RYASLYF
Sbjct: 11 SRQGKIRLKKWYTAMSAGEKAKIVKDLTPTILARKPKMCNIIEYNDHKVVYKRYASLYFI 70
Query: 185 CAVERE-DNELVTLEIIHRYVELLDKYFGSVCELDIIFNFEKAYFMLDEFMLA-GEVQET 358
+ + DNEL+TLEIIHR+VE +D YFG+VCELDIIFNF K Y +L+E ++ G + E+
Sbjct: 71 VGMTPDVDNELLTLEIIHRFVETMDTYFGNVCELDIIFNFSKVYDILNEMIMCDGSIAES 130
Query: 359 SKKNVLKAVDAQDMIQENED 418
S+K VL V D ++ N++
Sbjct: 131 SRKEVLHHVTVMDTMESNDN 150
>sp|P62743|AP2S1_MOUSE Clathrin coat assembly protein AP17 (Clathrin coat-associated
protein AP17) (Plasma membrane adaptor AP-2 17 kDa
protein) (Clathrin assembly protein 2 small chain)
sp|P62744|AP2S1_RAT Clathrin coat assembly protein AP17 (Clathrin coat-associated
protein AP17) (Plasma membrane adaptor AP-2 17 kDa
protein) (Clathrin assembly protein 2 small chain)
sp|Q5R940|AP2S1_PONPY Clathrin coat assembly protein AP17 (Clathrin coat-associated
protein AP17) (Plasma membrane adaptor AP-2 17 kDa
protein) (Clathrin assembly protein 2 small chain)
sp|P53680|AP2S1_HUMAN Clathrin coat assembly protein AP17 (Clathrin coat-associated
protein AP17) (Plasma membrane adaptor AP-2 17 kDa
protein) (HA2 17 kDa subunit) (Clathrin assembly protein
2 small chain)
Length = 142
Score = 138 bits (348), Expect = 1e-32
Identities = 62/125 (49%), Positives = 89/125 (71%)
Frame = +2
Query: 5 SRQGKVRLQKWYVAHSDKNRKKILRELTSLVLTRKPKMCAFIEWKDLKIVYRRYASLYFC 184
+R GK RL KWY+ D ++K++ E+ ++V R K F+E+++ KI+YRRYA LYFC
Sbjct: 9 NRAGKTRLAKWYMQFDDDEKQKLIEEVHAVVTVRDAKHTNFVEFRNFKIIYRRYAGLYFC 68
Query: 185 CAVEREDNELVTLEIIHRYVELLDKYFGSVCELDIIFNFEKAYFMLDEFMLAGEVQETSK 364
V+ DN L LE IH +VE+L++YF +VCELD++FNF K Y ++DE LAGE++ETS+
Sbjct: 69 ICVDVNDNNLAYLEAIHNFVEVLNEYFHNVCELDLVFNFYKVYTVVDEMFLAGEIRETSQ 128
Query: 365 KNVLK 379
VLK
Sbjct: 129 TKVLK 133
>sp|O50016|A2S1_MAIZE Clathrin coat assembly protein AP17 (Clathrin coat-associated
protein AP17) (Plasma membrane adaptor AP-2 17 kDa
protein) (Clathrin assembly protein 2 small chain)
Length = 132
Score = 132 bits (333), Expect = 7e-31
Identities = 64/123 (52%), Positives = 87/123 (70%), Gaps = 2/123 (1%)
Frame = +2
Query: 5 SRQGKVRLQKWYVAHSDKNRKKILRELTSLVLTRKPKMCAFIEWKDLKIVYRRYASLYFC 184
+RQGK RL K+YV D + K+ E+ LV+ R PK F+E++ K++YRRYA L+F
Sbjct: 9 NRQGKTRLAKYYVPLEDSEKHKVEYEVHRLVVNRDPKFTNFVEFRTHKVIYRRYAGLFFS 68
Query: 185 CAVEREDNELVTLEIIHRYVELLDKYFGSVCELDIIFNFEKA--YFMLDEFMLAGEVQET 358
V+ DNEL LE IH +VE+LD +F +VCELD++FNF K Y +LDEF+LAGE+QET
Sbjct: 69 ICVDITDNELAYLECIHLFVEILDHFFSNVCELDLVFNFHKVYRYLILDEFILAGELQET 128
Query: 359 SKK 367
SK+
Sbjct: 129 SKR 131
>sp|Q9WVL1|AP4S1_MOUSE Adapter-related protein complex 4 sigma 1 subunit (Sigma subunit of
AP-4) (AP-4 adapter complex sigma subunit)
Length = 144
Score = 113 bits (282), Expect = 6e-25
Identities = 55/137 (40%), Positives = 89/137 (64%), Gaps = 1/137 (0%)
Frame = +2
Query: 5 SRQGKVRLQKWYVAHSDKNRKKILR-ELTSLVLTRKPKMCAFIEWKDLKIVYRRYASLYF 181
++QG+ RL K+Y H D N++ +L E++ L+R + C+FIE+KD K++YR+YA+L+
Sbjct: 9 NKQGQTRLSKYY-EHVDINKRALLETEVSKSCLSRSSEQCSFIEYKDFKLIYRQYAALFV 67
Query: 182 CCAVEREDNELVTLEIIHRYVELLDKYFGSVCELDIIFNFEKAYFMLDEFMLAGEVQETS 361
V +NE+ E IH +VE+LD YF V ELDI+FN +K + +LDE +L G + ET+
Sbjct: 68 VVGVNDTENEMAIYEFIHNFVEVLDGYFSRVSELDIMFNLDKVHIILDEMVLNGCIVETN 127
Query: 362 KKNVLKAVDAQDMIQEN 412
+ +L + D + E+
Sbjct: 128 RARILAPLLILDKLSES 144
>sp|Q9Y587|AP4S1_HUMAN Adapter-related protein complex 4 sigma 1 subunit (Sigma subunit of
AP-4) (AP-4 adapter complex sigma subunit)
Length = 144
Score = 113 bits (282), Expect = 6e-25
Identities = 55/137 (40%), Positives = 89/137 (64%), Gaps = 1/137 (0%)
Frame = +2
Query: 5 SRQGKVRLQKWYVAHSDKNRKKILR-ELTSLVLTRKPKMCAFIEWKDLKIVYRRYASLYF 181
++QG+ RL K+Y H D N++ +L E+ L+R + C+FIE+KD K++YR+YA+L+
Sbjct: 9 NKQGQTRLSKYY-EHVDINKRTLLETEVIKSCLSRSNEQCSFIEYKDFKLIYRQYAALFI 67
Query: 182 CCAVEREDNELVTLEIIHRYVELLDKYFGSVCELDIIFNFEKAYFMLDEFMLAGEVQETS 361
V +NE+ E IH +VE+LD+YF V ELDI+FN +K + +LDE +L G + ET+
Sbjct: 68 VVGVNDTENEMAIYEFIHNFVEVLDEYFSRVSELDIMFNLDKVHIILDEMVLNGCIVETN 127
Query: 362 KKNVLKAVDAQDMIQEN 412
+ +L + D + E+
Sbjct: 128 RARILAPLLILDKMSES 144
>sp|Q00381|AP17_YEAST Clathrin coat assembly protein AP17 (Clathrin coat-associated
protein AP17) (Plasma membrane adaptor AP-2 17 kDa
protein) (Clathrin assembly protein 2 small chain)
Length = 147
Score = 110 bits (275), Expect = 4e-24
Identities = 57/138 (41%), Positives = 88/138 (63%), Gaps = 4/138 (2%)
Frame = +2
Query: 2 FSRQGKVRLQKWYVAHSDKNRKK--ILRELTSLVLTRKPK-MCAFIEWKD-LKIVYRRYA 169
F++QG VRL +W+ HS ++ + ++ L+ +R K F+E+ D K++YRRYA
Sbjct: 9 FNKQGVVRLVRWFDVHSSDPQRSQDAIAQIYRLISSRDHKHQSNFVEFSDSTKLIYRRYA 68
Query: 170 SLYFCCAVEREDNELVTLEIIHRYVELLDKYFGSVCELDIIFNFEKAYFMLDEFMLAGEV 349
LYF V+ D+E + L IH +VE+LD +FG+VCELDI+FNF K Y ++DE + GE+
Sbjct: 69 GLYFVMGVDLLDDEPIYLCHIHLFVEVLDAFFGNVCELDIVFNFYKVYMIMDEMFIGGEI 128
Query: 350 QETSKKNVLKAVDAQDMI 403
QE SK +L+ + D +
Sbjct: 129 QEISKDMLLERLSILDRL 146
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 69,495,895
Number of Sequences: 369166
Number of extensions: 1300318
Number of successful extensions: 3328
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3216
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3322
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6267895215
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)