Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_D14
(950 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q10580|SWAP_CAEEL SWAP protein (Suppressor of white apri... 79 2e-14
sp|Q12872|SFRS8_HUMAN Splicing factor, arginine/serine-rich... 74 8e-13
sp|P12297|SUWA_DROME Suppressor of white apricot protein 73 1e-12
sp|Q8K4Z5|SF3A1_MOUSE Splicing factor 3 subunit 1 (SF3a120) 49 2e-05
sp|Q15459|SF3A1_HUMAN Splicing factor 3 subunit 1 (Spliceos... 49 3e-05
sp|Q8CFC7|SFR16_MOUSE Splicing factor, arginine/serine-rich... 47 1e-04
sp|Q8N2M8|SFR16_HUMAN Splicing factor, arginine/serine-rich... 47 1e-04
sp|Q8IWZ8|SF04_HUMAN Splicing factor 4 (RNA-binding protein... 37 0.11
sp|Q8CH02|SF04_MOUSE Splicing factor 4 36 0.14
sp|Q8RXF1|SF3A1_ARATH Probable splicing factor 3 subunit 1 35 0.31
>sp|Q10580|SWAP_CAEEL SWAP protein (Suppressor of white apricot protein homolog)
Length = 749
Score = 79.0 bits (193), Expect = 2e-14
Identities = 48/140 (34%), Positives = 75/140 (53%), Gaps = 1/140 (0%)
Frame = +3
Query: 435 IDLQSACESPGNEDEYNSKRYDQSRYSAIHFDYDNNQEKNESQESKYEKEVEDNGTDAAY 614
+D+ + ++E +R DQ +AI FDY + K +S+ D +
Sbjct: 96 LDMYKDIQREQEKEEEEKRRNDQR--NAIGFDYGTGKVKARESDSE----------DEPF 143
Query: 615 LCPPNLKLLPGMITPATEREAAIIEQTAKFVADKGP*LEILIKAKQSNN-PKFVFLNFDH 791
P +K G+ P+ + IIE+TA F+ G +EI+IKAKQ NN +F FL FDH
Sbjct: 144 EPPEGIKFPVGLELPSNMKLHHIIEKTASFIVANGTQMEIVIKAKQRNNAEQFGFLEFDH 203
Query: 792 KLYPFYRHVTQLIKTAQYIP 851
+L PFY+++ +LI+ +YIP
Sbjct: 204 RLNPFYKYLQKLIREKKYIP 223
Score = 38.5 bits (88), Expect = 0.028
Identities = 25/77 (32%), Positives = 36/77 (46%), Gaps = 4/77 (5%)
Frame = +3
Query: 9 TLFQDDIKSKEINRGALLVPYNGDKSIMIDRFDCRGHLND----LQSHGGPFNELQLSHE 176
T+F +D +S+ I VP GD +DR+DCR L ++ +G P +
Sbjct: 26 TIFPNDYQSEHIAEERHTVPCLGDPENRVDRYDCRLLLPSIDVAIKRNGSPSEQCPTEAM 85
Query: 177 EWEVEQSCNFERYNDLY 227
E E C ERY D+Y
Sbjct: 86 E---EDMCEEERYLDMY 99
Score = 30.8 bits (68), Expect = 5.9
Identities = 34/135 (25%), Positives = 58/135 (42%), Gaps = 10/135 (7%)
Frame = +3
Query: 549 KNESQESKYEKEVEDNGTDAAYL----------CPPNLKLLPGMITPATEREAAIIEQTA 698
K +E+K EKE D+ D Y CP + P + PAT I+ A
Sbjct: 341 KMNVEEAKKEKE-NDHLDDPEYREWYENFYGRPCP---WIGPRPMIPATPDLEPILNSYA 396
Query: 699 KFVADKGP*LEILIKAKQSNNPKFVFLNFDHKLYPFYRHVTQLIKTAQYIPGKRHISHLV 878
+ VA +G E + A++ + + F+ Y +Y H ++ + Y P ++++S LV
Sbjct: 397 EHVAQRGLEAEASLAARE--DLQLHFMEPKSPYYSYYHHKVRMHQWRMYQPIEQNLSPLV 454
Query: 879 ESNGDQSQMGSFPQP 923
++ S P P
Sbjct: 455 LNSPAPPSAVSSPGP 469
>sp|Q12872|SFRS8_HUMAN Splicing factor, arginine/serine-rich 8 (Suppressor of white
apricot protein homolog)
Length = 951
Score = 73.6 bits (179), Expect = 8e-13
Identities = 48/145 (33%), Positives = 78/145 (53%), Gaps = 15/145 (10%)
Frame = +3
Query: 456 ESPGNEDEYNSKRYDQS-----RYSAIHF----DYDNNQEKNESQESKYEKE------VE 590
E E+EY KR+ ++ Y+A+ F DY + E E +E ++E +E
Sbjct: 125 EEARQEEEY--KRFSEALAEDGSYNAVGFTYGSDYYDPSEPTEEEEPSKQREKNEAENLE 182
Query: 591 DNGTDAAYLCPPNLKLLPGMITPATEREAAIIEQTAKFVADKGP*LEILIKAKQSNNPKF 770
+N + ++ P L + + P T + AIIE+TA FV +G EI++KAKQ+ N +F
Sbjct: 183 EN--EEPFVAPLGLSVPSDVELPPTAKMHAIIERTASFVCRQGAQFEIMLKAKQAPNSQF 240
Query: 771 VFLNFDHKLYPFYRHVTQLIKTAQY 845
FL FDH L P+Y+ + + +K +Y
Sbjct: 241 DFLRFDHYLNPYYKFIQKAMKEGRY 265
Score = 65.5 bits (158), Expect = 2e-10
Identities = 32/79 (40%), Positives = 50/79 (63%), Gaps = 4/79 (5%)
Frame = +3
Query: 3 SCTLFQDDIKSKEINRGALLVPYNGDKSIMIDRFDCRGHLNDLQSHGGPFN----ELQLS 170
+C LF+DD +++ +G L+P+ GD I+IDR+D RGHL+DL + ++ + QLS
Sbjct: 41 ACKLFRDDERAQAQEQGQHLIPWMGDHKILIDRYDGRGHLHDLSEYDAEYSTWNRDYQLS 100
Query: 171 HEEWEVEQSCNFERYNDLY 227
EE +E C+ ERY L+
Sbjct: 101 EEEARIEALCDEERYLALH 119
>sp|P12297|SUWA_DROME Suppressor of white apricot protein
Length = 963
Score = 72.8 bits (177), Expect = 1e-12
Identities = 44/120 (36%), Positives = 67/120 (55%), Gaps = 6/120 (5%)
Frame = +3
Query: 510 YSAIHFDYDNNQEKNES--QESKYEKEVEDNGTDAA--YLCPPNLKLLP--GMITPATER 671
+S + F YD + S S ++ N ++ ++ P L + P M P T +
Sbjct: 171 FSQVGFQYDGQSAASTSIGGSSTATSQLSPNSEESELPFVLPYTLMMAPPLDMQLPETMK 230
Query: 672 EAAIIEQTAKFVADKGP*LEILIKAKQSNNPKFVFLNFDHKLYPFYRHVTQLIKTAQYIP 851
+ AIIE+TA+F+A +G +EILIKAKQ+NN +F FL L P+YRH+ IK A++ P
Sbjct: 231 QHAIIEKTARFIATQGAQMEILIKAKQANNTQFDFLTQGGHLQPYYRHLLAAIKAAKFPP 290
Score = 66.2 bits (160), Expect = 1e-10
Identities = 36/84 (42%), Positives = 52/84 (61%), Gaps = 5/84 (5%)
Frame = +3
Query: 3 SCTLFQDDIKSKEINRGALLVPYNGDKSIMIDRFDCRGHLNDLQSH----GGPFNELQ-L 167
+C +F+DD K++E++ G L+P+ GD ++ IDR+D RG L +L H GG N L+ L
Sbjct: 66 ACKIFRDDEKAREMDHGKQLIPWMGDVNLKIDRYDVRGALCELAPHEAPPGGYGNRLEYL 125
Query: 168 SHEEWEVEQSCNFERYNDLYHTND 239
S EE EQ C ERY LY+ +
Sbjct: 126 SAEEQRAEQLCEEERYLFLYNNEE 149
>sp|Q8K4Z5|SF3A1_MOUSE Splicing factor 3 subunit 1 (SF3a120)
Length = 791
Score = 49.3 bits (116), Expect = 2e-05
Identities = 33/106 (31%), Positives = 50/106 (47%)
Frame = +3
Query: 621 PPNLKLLPGMITPATEREAAIIEQTAKFVADKGP*LEILIKAKQSNNPKFVFLNFDHKLY 800
P K + G+I P E I+++TA FVA GP E I+ + NNPKF FLN + +
Sbjct: 33 PTPSKPVVGIIYPPPEVRN-IVDKTASFVARNGPEFEARIRQNEINNPKFNFLNPNDPYH 91
Query: 801 PFYRHVTQLIKTAQYIPGKRHISHLVESNGDQSQMGSFPQPVAPKI 938
+YRH K + I +++ Q+ PQ V ++
Sbjct: 92 AYYRHKVSEFKEGKAQEPSAAIPKVMQQQ-QQATQQQLPQKVQAQV 136
>sp|Q15459|SF3A1_HUMAN Splicing factor 3 subunit 1 (Spliceosome associated protein 114)
(SAP 114) (SF3a120)
Length = 793
Score = 48.5 bits (114), Expect = 3e-05
Identities = 32/102 (31%), Positives = 49/102 (48%)
Frame = +3
Query: 633 KLLPGMITPATEREAAIIEQTAKFVADKGP*LEILIKAKQSNNPKFVFLNFDHKLYPFYR 812
K + G+I P E I+++TA FVA GP E I+ + NNPKF FLN + + +YR
Sbjct: 37 KPVVGIIYPPPEVRN-IVDKTASFVARNGPEFEARIRQNEINNPKFNFLNPNDPYHAYYR 95
Query: 813 HVTQLIKTAQYIPGKRHISHLVESNGDQSQMGSFPQPVAPKI 938
H K + I +++ Q+ PQ V ++
Sbjct: 96 HKVSEFKEGKAQEPSAAIPKVMQQQ-QQTTQQQLPQKVQAQV 136
>sp|Q8CFC7|SFR16_MOUSE Splicing factor, arginine/serine-rich 16 (Suppressor of
white-apricot homolog 2) (Clk4-associating SR-related
protein)
Length = 653
Score = 46.6 bits (109), Expect = 1e-04
Identities = 25/82 (30%), Positives = 41/82 (50%)
Frame = +3
Query: 3 SCTLFQDDIKSKEINRGALLVPYNGDKSIMIDRFDCRGHLNDLQSHGGPFNELQLSHEEW 182
+C + D + ++P+ GD + MIDRFD R HL+ + + P +S E+
Sbjct: 32 ACKVHLDSAVALAAESPVNMMPWQGDTNNMIDRFDVRAHLDHIPDYTPPL-LTTISPEQE 90
Query: 183 EVEQSCNFERYNDLYHTNDSDI 248
E+ CN+ERY L + + I
Sbjct: 91 SDERKCNYERYRGLVQNDFAGI 112
>sp|Q8N2M8|SFR16_HUMAN Splicing factor, arginine/serine-rich 16 (Suppressor of
white-apricot homolog 2)
Length = 659
Score = 46.6 bits (109), Expect = 1e-04
Identities = 25/82 (30%), Positives = 41/82 (50%)
Frame = +3
Query: 3 SCTLFQDDIKSKEINRGALLVPYNGDKSIMIDRFDCRGHLNDLQSHGGPFNELQLSHEEW 182
+C + D + ++P+ GD + MIDRFD R HL+ + + P +S E+
Sbjct: 32 ACKVHLDSAVALAAESPVNMMPWQGDTNNMIDRFDVRAHLDHIPDYTPPL-LTTISPEQE 90
Query: 183 EVEQSCNFERYNDLYHTNDSDI 248
E+ CN+ERY L + + I
Sbjct: 91 SDERKCNYERYRGLVQNDFAGI 112
>sp|Q8IWZ8|SF04_HUMAN Splicing factor 4 (RNA-binding protein RBP)
Length = 645
Score = 36.6 bits (83), Expect = 0.11
Identities = 30/102 (29%), Positives = 49/102 (48%), Gaps = 2/102 (1%)
Frame = +3
Query: 543 QEKNESQESKYEKEVEDNGTDAAYLCPPNLKLLPGMITPATEREAAIIEQTAKFVADKGP 722
Q +E +E YE+ +E +K+ P P +IE+ A+FVA+ GP
Sbjct: 162 QSPDEDEEEDYEQWLE-------------IKVSP----PEGAETRKVIEKLARFVAEGGP 204
Query: 723 *LEILIKAKQSNNPKFVFL-NFDHKLYPFYR-HVTQLIKTAQ 842
LE + +NP F FL + + + + +YR V ++ K AQ
Sbjct: 205 ELEKVAMEDYKDNPAFAFLHDKNSREFLYYRKKVAEIRKEAQ 246
Score = 35.0 bits (79), Expect = 0.31
Identities = 29/112 (25%), Positives = 49/112 (43%), Gaps = 7/112 (6%)
Frame = +3
Query: 528 DYDNN------QEKNESQESKYEKEVEDNGTDAAYLCPPNLKLLPGMITPATEREAAIIE 689
DY +N +KN + Y K+V + +A + K+ P P E + E
Sbjct: 213 DYKDNPAFAFLHDKNSREFLYYRKKVAEIRKEAQKSQAASQKVSP----PEDEEVKNLAE 268
Query: 690 QTAKFVADKGP*LEILIKAKQSNNPKFVFL-NFDHKLYPFYRHVTQLIKTAQ 842
+ A+F+AD GP +E + N F FL + + Y +YR + + A+
Sbjct: 269 KLARFIADGGPEVETIALQNNRENQAFSFLYEPNSQGYKYYRQKLEEFRKAK 320
>sp|Q8CH02|SF04_MOUSE Splicing factor 4
Length = 643
Score = 36.2 bits (82), Expect = 0.14
Identities = 31/103 (30%), Positives = 47/103 (45%), Gaps = 3/103 (2%)
Frame = +3
Query: 543 QEKNESQESKYEKEVEDNGTDAAYLCPPNLKLLPGMITPATEREAAIIEQTAKFVADKGP 722
Q ++ +E YE+ +E +K+ P P +IE+ A+FVA+ GP
Sbjct: 158 QSPDDDEEEDYEQWLE-------------IKVSP----PEGAETRRVIEKLARFVAEGGP 200
Query: 723 *LEILIKAKQSNNPKFVFL---NFDHKLYPFYRHVTQLIKTAQ 842
LE + +NP F FL N LY + R V ++ K AQ
Sbjct: 201 ELEKVAMEDYKDNPAFTFLHDKNSREFLY-YRRKVAEIRKEAQ 242
Score = 33.1 bits (74), Expect = 1.2
Identities = 37/153 (24%), Positives = 60/153 (39%), Gaps = 18/153 (11%)
Frame = +3
Query: 528 DYDNN------QEKNESQESKYEKEVEDNGTDAAYLCPPNLKLLPGMITPATEREAAIIE 689
DY +N +KN + Y ++V + +A K+ P P E + E
Sbjct: 209 DYKDNPAFTFLHDKNSREFLYYRRKVAEIRKEAQKPQAATQKVSP----PEDEEAKNLAE 264
Query: 690 QTAKFVADKGP*LEILIKAKQSNNPKFVFL-NFDHKLYPFYRHVTQLIKTAQY------- 845
+ A+F+AD GP +E + N F FL + + + Y +YR + A+
Sbjct: 265 KLARFIADGGPEVETIALQNNRENQAFSFLYDPNSQGYRYYRQKLDEFRKAKAGSTGSFP 324
Query: 846 ----IPGKRHISHLVESNGDQSQMGSFPQPVAP 932
P R S +G + + P PVAP
Sbjct: 325 APAPNPSLRRKSAPEALSGAVPPITACPTPVAP 357
>sp|Q8RXF1|SF3A1_ARATH Probable splicing factor 3 subunit 1
Length = 785
Score = 35.0 bits (79), Expect = 0.31
Identities = 17/49 (34%), Positives = 28/49 (57%)
Frame = +3
Query: 663 TEREAAIIEQTAKFVADKGP*LEILIKAKQSNNPKFVFLNFDHKLYPFY 809
T E II+ TA+FVA G + +++NNP+F F+ H ++ F+
Sbjct: 187 TGEELDIIKLTAQFVARNGKSFLTGLSNRENNNPQFHFMKPTHSMFTFF 235
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 97,428,784
Number of Sequences: 369166
Number of extensions: 1807331
Number of successful extensions: 5171
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4959
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5159
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9895030780
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)