Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_C15
(882 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P32783|ABD1_YEAST mRNA (guanine-N(7)-)-methyltransferase 114 3e-25
sp|Q8SR66|MCES_ENCCU mRNA cap guanine-N7 methyltransferase ... 110 5e-24
sp|Q9JFA8|MCEL_VACCT mRNA capping enzyme large subunit [Inc... 50 6e-06
sp|P04298|MCEL_VACCV mRNA capping enzyme large subunit [Inc... 50 8e-06
sp|P20979|MCEL_VACCC mRNA capping enzyme large subunit [Inc... 50 8e-06
sp|Q80DX6|MCEL_CWPXG mRNA capping enzyme large subunit [Inc... 50 8e-06
sp|O57209|MCEL_VACCA mRNA capping enzyme large subunit [Inc... 50 8e-06
sp|Q8QMV9|MCEL_CWPXB mRNA capping enzyme large subunit [Inc... 50 8e-06
sp|P25950|MCEL_SFVKA mRNA capping enzyme large subunit [Inc... 50 1e-05
sp|Q8V2R8|MCEL_CAMPM mRNA capping enzyme large subunit [Inc... 49 2e-05
>sp|P32783|ABD1_YEAST mRNA (guanine-N(7)-)-methyltransferase
Length = 436
Score = 114 bits (286), Expect = 3e-25
Identities = 82/254 (32%), Positives = 128/254 (50%), Gaps = 19/254 (7%)
Frame = +3
Query: 27 AARNESNIIYLRNFNNWIKSVLIENVVNDASKECKGISVLDMGSGKGGDQLKWTQHGVSH 206
A RN S II LRNFNN IK +LI+ G VL++G GKGGD K+ G+S
Sbjct: 136 AKRNLSPIIKLRNFNNAIKYMLIDKYTKP------GDVVLELGCGKGGDLRKYGAAGISQ 189
Query: 207 VTFLDIAEESVNQSQERFKKRKFSYTADFHVADFTKEILKSILSRSYE----------II 356
+DI+ S+ ++ +R++ + D+ V T + L + E I+
Sbjct: 190 FIGIDISNASIQEAHKRYRSMR---NLDYQVVLITGDCFGESLGVAVEPFPDCRFPCDIV 246
Query: 357 SCQFAFHYSFESYEQANLTFKNVSDQLSEHGYFMGTTVNG----FELIKRLKRSDKNSFG 524
S QF HY+FE+ E+A NV+ L G+F GT + ++L K K +K S+G
Sbjct: 247 STQFCLHYAFETEEKARRALLNVAKSLKIGGHFFGTIPDSEFIRYKLNKFPKEVEKPSWG 306
Query: 525 NDIYKVSFPSEKNVDN----ISLFGSKYNFYLDGSVD-CPEFVVYPPLMDHLALQNKMKL 689
N IYKV+F + N S +G Y ++L+ ++D PE+VV + LA + ++L
Sbjct: 307 NSIYKVTFENNSYQKNDYEFTSPYGQMYTYWLEDAIDNVPEYVVPFETLRSLADEYGLEL 366
Query: 690 ISRRSLPEFYEDNI 731
+S+ +F+ I
Sbjct: 367 VSQMPFNKFFVQEI 380
>sp|Q8SR66|MCES_ENCCU mRNA cap guanine-N7 methyltransferase (mRNA
(guanine-N(7)-)-methyltransferase)
Length = 298
Score = 110 bits (275), Expect = 5e-24
Identities = 76/238 (31%), Positives = 122/238 (51%), Gaps = 1/238 (0%)
Frame = +3
Query: 15 EVGVAARNESNIIYLRNFNNWIKSVLIENVVNDASKECKGISVLDMGSGKGGDQLKWTQH 194
E G +R S I +RN NN+IK+ LI +G SVLD+G GKGGD LK+ +
Sbjct: 32 ERGRESRQRSKTINIRNANNFIKACLIRLYTK------RGDSVLDLGCGKGGDLLKYERA 85
Query: 195 GVSHVTFLDIAEESVNQSQERFKKRKFSYTADFHVADFTKEILKSILSRSYEIISCQFAF 374
G+ +DIAE S+N ++ R + K + F D + L + +++IS QF+F
Sbjct: 86 GIGEYYGVDIAEVSINDARVRARNMKRRFKVFFRAQDSYGRHMD--LGKEFDVISSQFSF 143
Query: 375 HYSFESYEQANLTFKNVSDQLSEHGYFMGTTVNGFELIKRLKRSDKNSFGNDIYKVSFPS 554
HY+F + E ++ +N++ L GYF+ T + +++R K + ND YK+
Sbjct: 144 HYAFSTSESLDIAQRNIARHLRPGGYFIMTVPSRDVILERYK---QGRMSNDFYKIELEK 200
Query: 555 EKNVDNISLFGSKYNFYLDGSV-DCPEFVVYPPLMDHLALQNKMKLISRRSLPEFYED 725
++V S+ +Y F L SV +C E+ V M + + L+ R+ +FYED
Sbjct: 201 MEDVPMESV--REYRFTLLDSVNNCIEYFVDFTRMVDGFKRLGLSLVERKGFIDFYED 256
>sp|Q9JFA8|MCEL_VACCT mRNA capping enzyme large subunit [Includes: Polynucleotide
5'-triphosphatase (mRNA 5'-triphosphatase) (TPase); mRNA
guanylyltransferase (GTP--RNA guanylyltransferase)
(GTase)]
Length = 844
Score = 50.4 bits (119), Expect = 6e-06
Identities = 44/180 (24%), Positives = 84/180 (46%), Gaps = 15/180 (8%)
Frame = +3
Query: 69 NNWIKSVLI-----ENVVNDASKECKGISVLDMGSGKGGDQLKWTQHGVSHVTFLDIAEE 233
+N++K++LI + ++D++K VL + G G D K+ ++ + D +
Sbjct: 569 SNYVKTLLISMYCSKTFLDDSNKR----KVLAIDFGNGADLEKYFYGEIALLVATDPDAD 624
Query: 234 SVNQSQERFKKRKFSYTADFHVADFTKEILKS----------ILSRSYEIISCQFAFHYS 383
++ + ER+ K ++ D+ +E ++S ++II QFA HYS
Sbjct: 625 AIARGNERYNKLNSGIKTKYYKFDYIQETIRSDTFVSSVREVFYFGKFKIIDWQFAIHYS 684
Query: 384 FESYEQANLTFKNVSDQLSEHGYFMGTTVNGFELIKRLKRSDKNSFGNDIYKVSFPSEKN 563
F A + N+S+ + G + TT++G +L K +DK +F I + PS +N
Sbjct: 685 FHPRHYATV-MNNLSELTASGGKVLITTMDGDKL---SKLTDKKTF---IIHKNLPSSEN 737
>sp|P04298|MCEL_VACCV mRNA capping enzyme large subunit [Includes: Polynucleotide
5'-triphosphatase (mRNA 5'-triphosphatase) (TPase); mRNA
guanylyltransferase (GTP--RNA guanylyltransferase)
(GTase)]
Length = 844
Score = 50.1 bits (118), Expect = 8e-06
Identities = 44/180 (24%), Positives = 83/180 (46%), Gaps = 15/180 (8%)
Frame = +3
Query: 69 NNWIKSVLI-----ENVVNDASKECKGISVLDMGSGKGGDQLKWTQHGVSHVTFLDIAEE 233
+N++K++LI + ++D++K VL + G G D K+ ++ + D +
Sbjct: 569 SNYVKTLLISMYCSKTFLDDSNKR----KVLAIDFGNGADLEKYFYGEIALLVATDPDAD 624
Query: 234 SVNQSQERFKKRKFSYTADFHVADFTKEILKS----------ILSRSYEIISCQFAFHYS 383
++ + ER+ K ++ D+ +E ++S + II QFA HYS
Sbjct: 625 AIARGNERYNKLNSGIKTKYYKFDYIQETIRSDTFVSSVREVFYFGKFNIIDWQFAIHYS 684
Query: 384 FESYEQANLTFKNVSDQLSEHGYFMGTTVNGFELIKRLKRSDKNSFGNDIYKVSFPSEKN 563
F A + N+S+ + G + TT++G +L K +DK +F I + PS +N
Sbjct: 685 FHPRHYATV-MNNLSELTASGGKVLITTMDGDKL---SKLTDKKTF---IIHKNLPSSEN 737
>sp|P20979|MCEL_VACCC mRNA capping enzyme large subunit [Includes: Polynucleotide
5'-triphosphatase (mRNA 5'-triphosphatase) (TPase); mRNA
guanylyltransferase (GTP--RNA guanylyltransferase)
(GTase)]
Length = 844
Score = 50.1 bits (118), Expect = 8e-06
Identities = 44/180 (24%), Positives = 83/180 (46%), Gaps = 15/180 (8%)
Frame = +3
Query: 69 NNWIKSVLI-----ENVVNDASKECKGISVLDMGSGKGGDQLKWTQHGVSHVTFLDIAEE 233
+N++K++LI + ++D++K VL + G G D K+ ++ + D +
Sbjct: 569 SNYVKTLLISMYCSKTFLDDSNKR----KVLAIDFGNGADLEKYFYGEIALLVATDPDAD 624
Query: 234 SVNQSQERFKKRKFSYTADFHVADFTKEILKS----------ILSRSYEIISCQFAFHYS 383
++ + ER+ K ++ D+ +E ++S + II QFA HYS
Sbjct: 625 AIARGNERYNKLNSGIKTKYYKFDYIQETIRSDTFVSSVREVFYFGKFNIIDWQFAIHYS 684
Query: 384 FESYEQANLTFKNVSDQLSEHGYFMGTTVNGFELIKRLKRSDKNSFGNDIYKVSFPSEKN 563
F A + N+S+ + G + TT++G +L K +DK +F I + PS +N
Sbjct: 685 FHPRHYATV-MNNLSELTASGGKVLITTMDGDKL---SKLTDKKTF---IIHKNLPSSEN 737
>sp|Q80DX6|MCEL_CWPXG mRNA capping enzyme large subunit [Includes: Polynucleotide
5'-triphosphatase (mRNA 5'-triphosphatase) (TPase); mRNA
guanylyltransferase (GTP--RNA guanylyltransferase)
(GTase)]
Length = 844
Score = 50.1 bits (118), Expect = 8e-06
Identities = 44/180 (24%), Positives = 83/180 (46%), Gaps = 15/180 (8%)
Frame = +3
Query: 69 NNWIKSVLI-----ENVVNDASKECKGISVLDMGSGKGGDQLKWTQHGVSHVTFLDIAEE 233
+N++K++LI + ++D++K VL + G G D K+ ++ + D +
Sbjct: 569 SNYVKTLLISMYCSKTFLDDSNKR----KVLAIDFGNGADLEKYFYGEIALLVATDPDAD 624
Query: 234 SVNQSQERFKKRKFSYTADFHVADFTKEILKS----------ILSRSYEIISCQFAFHYS 383
++ + ER+ K ++ D+ +E ++S + II QFA HYS
Sbjct: 625 AIARGNERYNKLNSGIKTKYYKFDYIQETIRSDTFVSSVREVFYFGKFNIIDWQFAIHYS 684
Query: 384 FESYEQANLTFKNVSDQLSEHGYFMGTTVNGFELIKRLKRSDKNSFGNDIYKVSFPSEKN 563
F A + N+S+ + G + TT++G +L K +DK +F I + PS +N
Sbjct: 685 FHPRHYATV-MNNLSELTASGGKVLITTMDGDKL---SKLTDKKTF---IIHKNLPSSEN 737
>sp|O57209|MCEL_VACCA mRNA capping enzyme large subunit [Includes: Polynucleotide
5'-triphosphatase (mRNA 5'-triphosphatase) (TPase); mRNA
guanylyltransferase (GTP--RNA guanylyltransferase)
(GTase)]
Length = 844
Score = 50.1 bits (118), Expect = 8e-06
Identities = 44/180 (24%), Positives = 83/180 (46%), Gaps = 15/180 (8%)
Frame = +3
Query: 69 NNWIKSVLI-----ENVVNDASKECKGISVLDMGSGKGGDQLKWTQHGVSHVTFLDIAEE 233
+N++K++LI + ++D++K VL + G G D K+ ++ + D +
Sbjct: 569 SNYVKTLLISMYCSKTFLDDSNKR----KVLAIDFGNGADLEKYFYGEIALLVATDPDAD 624
Query: 234 SVNQSQERFKKRKFSYTADFHVADFTKEILKS----------ILSRSYEIISCQFAFHYS 383
++ + ER+ K ++ D+ +E ++S + II QFA HYS
Sbjct: 625 AIARGNERYNKLNSGIKTKYYKFDYIQETIRSDTFVSSVREVFYFGKFNIIDWQFAIHYS 684
Query: 384 FESYEQANLTFKNVSDQLSEHGYFMGTTVNGFELIKRLKRSDKNSFGNDIYKVSFPSEKN 563
F A + N+S+ + G + TT++G +L K +DK +F I + PS +N
Sbjct: 685 FHPRHYATV-MNNLSELTASGGKVLITTMDGDKL---SKLTDKKTF---IIHKNLPSSEN 737
>sp|Q8QMV9|MCEL_CWPXB mRNA capping enzyme large subunit [Includes: Polynucleotide
5'-triphosphatase (mRNA 5'-triphosphatase) (TPase); mRNA
guanylyltransferase (GTP--RNA guanylyltransferase)
(GTase)]
Length = 844
Score = 50.1 bits (118), Expect = 8e-06
Identities = 44/180 (24%), Positives = 83/180 (46%), Gaps = 15/180 (8%)
Frame = +3
Query: 69 NNWIKSVLI-----ENVVNDASKECKGISVLDMGSGKGGDQLKWTQHGVSHVTFLDIAEE 233
+N++K++LI + ++D++K VL + G G D K+ ++ + D +
Sbjct: 569 SNYVKTLLISMYCSKTFLDDSNKR----KVLAIDFGNGADLEKYFYGEIALLVATDPDAD 624
Query: 234 SVNQSQERFKKRKFSYTADFHVADFTKEILKS----------ILSRSYEIISCQFAFHYS 383
++ + ER+ K ++ D+ +E ++S + II QFA HYS
Sbjct: 625 AIARGNERYNKLNSGIKTKYYKFDYIQETIRSDTFVSSVREVFYFGKFNIIDWQFAIHYS 684
Query: 384 FESYEQANLTFKNVSDQLSEHGYFMGTTVNGFELIKRLKRSDKNSFGNDIYKVSFPSEKN 563
F A + N+S+ + G + TT++G +L K +DK +F I + PS +N
Sbjct: 685 FHPRHYATV-MNNLSELTASGGKVLITTMDGDKL---SKLTDKKTF---IIHKNLPSSEN 737
>sp|P25950|MCEL_SFVKA mRNA capping enzyme large subunit [Includes: Polynucleotide
5'-triphosphatase (mRNA 5'-triphosphatase) (TPase); mRNA
guanylyltransferase (GTP--RNA guanylyltransferase)
(GTase)]
Length = 836
Score = 49.7 bits (117), Expect = 1e-05
Identities = 41/180 (22%), Positives = 89/180 (49%), Gaps = 15/180 (8%)
Frame = +3
Query: 69 NNWIKSVLI-----ENVVNDASKECKGISVLDMGSGKGGDQLKWTQHGVSHVTFLDIAEE 233
+N++K++LI + +++++K VL + G G D K+ +S + D +E
Sbjct: 561 SNYVKTLLISLYCSKTFLDNSNKR----KVLAIDFGNGADLEKYFYGEISSLVATDPDKE 616
Query: 234 SVNQSQERFKKRKFSYTADFHVADFTKEILKSILSRS----------YEIISCQFAFHYS 383
++ + ER+ + ++ D+ +E ++S+ S ++++ QFA HYS
Sbjct: 617 AIGRCIERYNSLNSGIKSKYYKFDYIQETIRSVTYVSSVREVFFFGKFDLVDWQFAIHYS 676
Query: 384 FESYEQANLTFKNVSDQLSEHGYFMGTTVNGFELIKRLKRSDKNSFGNDIYKVSFPSEKN 563
F A + N+++ + G + TT++G +L+ +L +DK +F + + PS +N
Sbjct: 677 FHPKHYATV-MNNLTELTASGGKVLITTMDG-DLLSQL--TDKKTF---VIHKNLPSSEN 729
>sp|Q8V2R8|MCEL_CAMPM mRNA capping enzyme large subunit [Includes: Polynucleotide
5'-triphosphatase (mRNA 5'-triphosphatase) (TPase); mRNA
guanylyltransferase (GTP--RNA guanylyltransferase)
(GTase)]
sp|Q775U0|MCEL_CAMPS mRNA capping enzyme large subunit [Includes: Polynucleotide
5'-triphosphatase (mRNA 5'-triphosphatase) (TPase); mRNA
guanylyltransferase (GTP--RNA guanylyltransferase)
(GTase)]
Length = 844
Score = 48.9 bits (115), Expect = 2e-05
Identities = 43/180 (23%), Positives = 83/180 (46%), Gaps = 15/180 (8%)
Frame = +3
Query: 69 NNWIKSVLI-----ENVVNDASKECKGISVLDMGSGKGGDQLKWTQHGVSHVTFLDIAEE 233
+N++K++LI + ++D++K VL + G G D K+ ++ + D +
Sbjct: 569 SNYVKTLLISMYCSKTFLDDSNKR----KVLAIDFGNGADLEKYFYGEIALLVATDPDAD 624
Query: 234 SVNQSQERFKKRKFSYTADFHVADFTKEILKS----------ILSRSYEIISCQFAFHYS 383
++ + ER+ + ++ D+ +E ++S + II QFA HYS
Sbjct: 625 AIARGNERYNRLNSGIKTKYYKFDYIQETIRSDTFVSSVREVFYFGKFNIIDWQFAIHYS 684
Query: 384 FESYEQANLTFKNVSDQLSEHGYFMGTTVNGFELIKRLKRSDKNSFGNDIYKVSFPSEKN 563
F A + N+S+ + G + TT++G +L K +DK +F I + PS +N
Sbjct: 685 FHPRHYATV-MNNLSELTASGGKVLITTMDGDKL---SKLTDKKTF---IIHKNLPSSEN 737
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 89,517,864
Number of Sequences: 369166
Number of extensions: 1741342
Number of successful extensions: 5748
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5500
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5731
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8790245790
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)