Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_B14-2
(517 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9Y4K3|TRAF6_HUMAN TNF receptor-associated factor 6 (Int... 59 7e-09
sp|P70196|TRAF6_MOUSE TNF receptor-associated factor 6 55 1e-07
sp|P70191|TRAF5_MOUSE TNF receptor-associated factor 5 52 8e-07
sp|O00463|TRAF5_HUMAN TNF receptor-associated factor 5 (RIN... 47 3e-05
sp|Q9BUZ4|TRAF4_HUMAN TNF receptor-associated factor 4 (Cys... 46 5e-05
sp|Q61382|TRAF4_MOUSE TNF receptor-associated factor 4 (Cys... 44 2e-04
sp|O70263|LNX1_MOUSE Ubiquitin ligase LNX (Numb-binding pro... 44 2e-04
sp|Q8TBB1|LNX1_HUMAN Ubiquitin ligase LNX (Numb-binding pro... 43 5e-04
sp|O76064|RNF8_HUMAN Ubiquitin ligase protein RNF8 (RING fi... 43 5e-04
sp|P39429|TRAF2_MOUSE TNF receptor-associated factor 2 42 9e-04
>sp|Q9Y4K3|TRAF6_HUMAN TNF receptor-associated factor 6 (Interleukin 1 signal transducer)
(RING finger protein 85)
Length = 522
Score = 58.9 bits (141), Expect = 7e-09
Identities = 36/117 (30%), Positives = 55/117 (47%), Gaps = 1/117 (0%)
Frame = -2
Query: 429 KCIICKEILVNAQQSLCGCHFCLVCI-NNYKNNGYKHCPGNTPECKSEDLLTISFDPIIN 253
+C IC L A Q+ CG FC CI + ++ G+K CP + L +F
Sbjct: 69 ECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHK-CPVDNEILLENQLFPDNF---AK 124
Query: 252 KRISNLIARCPHERCFHECRLIEMEDHMRFCSTGNVNCPFNNVGCEHEEMNIDKLND 82
+ I +L+ +CP+E C H+ L +EDH C ++CP + +NI L D
Sbjct: 125 REILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALMDCPQCQRPFQKFHINIHILKD 181
Score = 28.9 bits (63), Expect = 7.6
Identities = 14/47 (29%), Positives = 23/47 (48%)
Frame = -2
Query: 162 CSTGNVNCPFNNVGCEHEEMNIDKLNDHMLLEIVNHNKILIDIIGHL 22
C T + C F+ GC HE+M + L H+ +H ++L + L
Sbjct: 235 CPTAPIPCTFSTFGC-HEKMQRNHLARHLQENTQSHMRMLAQAVHSL 280
>sp|P70196|TRAF6_MOUSE TNF receptor-associated factor 6
Length = 530
Score = 55.1 bits (131), Expect = 1e-07
Identities = 32/99 (32%), Positives = 47/99 (47%), Gaps = 1/99 (1%)
Frame = -2
Query: 429 KCIICKEILVNAQQSLCGCHFCLVCI-NNYKNNGYKHCPGNTPECKSEDLLTISFDPIIN 253
+C IC L A Q+ CG FC CI + ++ G+K CP + L +F
Sbjct: 69 ECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHK-CPVDNEILLENQLFPDNF---AK 124
Query: 252 KRISNLIARCPHERCFHECRLIEMEDHMRFCSTGNVNCP 136
+ I +L +CP++ C + L +EDH C VNCP
Sbjct: 125 REILSLTVKCPNKGCLQKMELRHLEDHQVHCEFALVNCP 163
>sp|P70191|TRAF5_MOUSE TNF receptor-associated factor 5
Length = 558
Score = 52.0 bits (123), Expect = 8e-07
Identities = 38/135 (28%), Positives = 58/135 (42%), Gaps = 2/135 (1%)
Frame = -2
Query: 474 SEYKFTTVNDSSEDLKCIICKEILVNAQQSLCGCHFCLVCINNYKN-NGYKHCPGNTPEC 298
+EY+F V E KC C +L N Q+ CG FC CI + + N CP +
Sbjct: 31 TEYQF--VEQLEERYKCAFCHSVLHNPHQTGCGHRFCQQCIRSLRELNSVPICPVDKEVI 88
Query: 297 KSEDLLTISFDPIINKRISNLIARCPH-ERCFHECRLIEMEDHMRFCSTGNVNCPFNNVG 121
K +++ D + + NL C + C L +DH++ CS V CP N
Sbjct: 89 KPQEVFK---DNCCKREVLNLHVYCKNAPGCNARIILGRFQDHLQHCSFQAVPCP--NES 143
Query: 120 CEHEEMNIDKLNDHM 76
C + D + +H+
Sbjct: 144 CREAMLRKD-VKEHL 157
Score = 34.3 bits (77), Expect = 0.18
Identities = 20/62 (32%), Positives = 26/62 (41%), Gaps = 10/62 (16%)
Frame = -2
Query: 225 CPHERCFHECRLIEMEDHMRFCSTGNVNCPFNNVGC----------EHEEMNIDKLNDHM 76
CP+ RC + +H+ C +CPF + GC EHE L DHM
Sbjct: 195 CPN-RCVQTIPRARVNEHLTVCPEAEQDCPFKHYGCTVKGKRGNLLEHERA---ALQDHM 250
Query: 75 LL 70
LL
Sbjct: 251 LL 252
>sp|O00463|TRAF5_HUMAN TNF receptor-associated factor 5 (RING finger protein 84)
Length = 557
Score = 47.0 bits (110), Expect = 3e-05
Identities = 38/134 (28%), Positives = 58/134 (43%), Gaps = 2/134 (1%)
Frame = -2
Query: 471 EYKFTTVNDSSEDLKCIICKEILVNAQQSLCGCHFCLVCINNYKN-NGYKHCPGNTPECK 295
EY+F V E KC C +L N Q+ CG FC CI + + N CP + K
Sbjct: 32 EYQF--VERLEERYKCAFCHSVLHNPHQTGCGHRFCQHCILSLRELNTVPICPVDKEVIK 89
Query: 294 SEDLLTISFDPIINKRISNLIARCPH-ERCFHECRLIEMEDHMRFCSTGNVNCPFNNVGC 118
S+++ D + + NL C + C + L +DH++ C V C +N C
Sbjct: 90 SQEVFK---DNCCKREVLNLYVYCSNAPGCNAKVILGRYQDHLQQCLFQPVQC--SNEKC 144
Query: 117 EHEEMNIDKLNDHM 76
+ D L +H+
Sbjct: 145 REPVLRKD-LKEHL 157
>sp|Q9BUZ4|TRAF4_HUMAN TNF receptor-associated factor 4 (Cysteine-rich domain associated
with RING and Traf domains protein 1) (Malignant 62)
(RING finger protein 83)
Length = 470
Score = 46.2 bits (108), Expect = 5e-05
Identities = 34/120 (28%), Positives = 50/120 (41%), Gaps = 8/120 (6%)
Frame = -2
Query: 471 EYKFTTVNDSSEDLKCIIC-KEILVNAQQSLCGCHFCLVCINNYKNNGYKHCPGNTPECK 295
+YKF + L C +C K + Q S CG FC C+ + + G CP
Sbjct: 5 DYKF--LEKPKRRLLCPLCGKPMREPVQVSTCGHRFCDTCLQEFLSEGVFKCP------- 55
Query: 294 SEDLLTISF-----DPIINKRISNLIARCPH--ERCFHECRLIEMEDHMRFCSTGNVNCP 136
ED L + + DP + ++ L RC H E C L ++ H+ CS + CP
Sbjct: 56 -EDQLPLDYAKIYPDPELEVQVLGLPIRCIHSEEGCRWSGPLRHLQGHLNTCSFNVIPCP 114
Score = 32.0 bits (71), Expect = 0.89
Identities = 25/110 (22%), Positives = 48/110 (43%), Gaps = 3/110 (2%)
Frame = -2
Query: 321 CPGNTPECKSEDLLTISFDPIINKRIS--NLIARCPHERCFHECRLIEMEDHMR-FCSTG 151
CP T C + FD I + + L CP++ ++ H++ C+T
Sbjct: 188 CPKRTQPC-TYCTKEFVFDTIQSHQYQCPRLPVACPNQCGVGTVAREDLPGHLKDSCNTA 246
Query: 150 NVNCPFNNVGCEHEEMNIDKLNDHMLLEIVNHNKILIDIIGHLRNDVREL 1
V CPF + GC+H + + H+ + H ++ ++ R +++EL
Sbjct: 247 LVLCPFKDSGCKHRCPKL-AMARHVEESVKPHLAMMCALVSRQRQELQEL 295
>sp|Q61382|TRAF4_MOUSE TNF receptor-associated factor 4 (Cysteine-rich motif associated to
RING and Traf domains protein 1)
Length = 470
Score = 43.9 bits (102), Expect = 2e-04
Identities = 33/120 (27%), Positives = 50/120 (41%), Gaps = 8/120 (6%)
Frame = -2
Query: 471 EYKFTTVNDSSEDLKCIIC-KEILVNAQQSLCGCHFCLVCINNYKNNGYKHCPGNTPECK 295
+YKF L C +C K + + + S CG +C C+ + + G CP
Sbjct: 5 DYKFP--EKPKRRLLCPLCGKPMRDSVEVSTCGHRYCDNCLQEFLSEGVFKCP------- 55
Query: 294 SEDLLTISF-----DPIINKRISNLIARCPH--ERCFHECRLIEMEDHMRFCSTGNVNCP 136
ED L + + DP + ++ L RC H E C L ++ H+ CS V CP
Sbjct: 56 -EDQLPLDYAKIYPDPELEVQVLGLAIRCIHSEEGCRWSGPLRHLQGHLNTCSFNVVPCP 114
Score = 30.4 bits (67), Expect = 2.6
Identities = 17/76 (22%), Positives = 35/76 (46%), Gaps = 1/76 (1%)
Frame = -2
Query: 225 CPHERCFHECRLIEMEDHMR-FCSTGNVNCPFNNVGCEHEEMNIDKLNDHMLLEIVNHNK 49
CP++ ++ H++ C T V CPF GC+H + + H+ + H
Sbjct: 221 CPNQCGVGTVAREDLPTHLKDSCRTAFVLCPFKESGCKHRCPKL-AMGRHVEESVKPHLA 279
Query: 48 ILIDIIGHLRNDVREL 1
++ ++ R +++EL
Sbjct: 280 MMCALVSRQRQELQEL 295
>sp|O70263|LNX1_MOUSE Ubiquitin ligase LNX (Numb-binding protein 1) (Ligand of
Numb-binding protein 1) (Ligand of Numb-protein X 1)
Length = 728
Score = 43.9 bits (102), Expect = 2e-04
Identities = 27/110 (24%), Positives = 48/110 (43%), Gaps = 6/110 (5%)
Frame = -2
Query: 462 FTTVNDSSEDLKCIICKEILVNAQQSLCGCHFCLVCINNYKNNGYKHCPGN-----TPEC 298
+T D +DL C IC + L++ + CG +C +C+ N+ CP + C
Sbjct: 33 YTYTEDVDDDLICHICLQALLDPLDTPCGHTYCTLCLTNFLVE-KDFCPVDRKPVVLQHC 91
Query: 297 KSEDLLTISFDPIINKRISNLIARCPH-ERCFHECRLIEMEDHMRFCSTG 151
K +L +NK ++ L+ CP E C + +++ H + G
Sbjct: 92 KKSSIL-------VNKLLNKLLVTCPFTEHCTEVLQRCDLQHHFQTSCKG 134
>sp|Q8TBB1|LNX1_HUMAN Ubiquitin ligase LNX (Numb-binding protein 1) (Ligand of
Numb-protein X 1)
Length = 728
Score = 42.7 bits (99), Expect = 5e-04
Identities = 27/118 (22%), Positives = 52/118 (44%), Gaps = 6/118 (5%)
Frame = -2
Query: 486 YNLLSEYKFTTVNDSSEDLKCIICKEILVNAQQSLCGCHFCLVCINNYKNNGYKHCPGN- 310
++L + ++ + +DL C IC + L++ + CG +C +C+ N+ CP +
Sbjct: 21 HSLEENHFYSYPEEVDDDLICHICLQALLDPLDTPCGHTYCTLCLTNFLVE-KDFCPMDR 79
Query: 309 ----TPECKSEDLLTISFDPIINKRISNLIARCP-HERCFHECRLIEMEDHMRFCSTG 151
CK +L +NK ++ L+ CP E C + ++E H + G
Sbjct: 80 KPLVLQHCKKSSIL-------VNKLLNKLLVTCPFREHCTQVLQRCDLEHHFQTSCKG 130
>sp|O76064|RNF8_HUMAN Ubiquitin ligase protein RNF8 (RING finger protein 8)
Length = 485
Score = 42.7 bits (99), Expect = 5e-04
Identities = 27/82 (32%), Positives = 40/82 (48%), Gaps = 1/82 (1%)
Frame = -2
Query: 453 VNDSSE-DLKCIICKEILVNAQQSLCGCHFCLVCINNYKNNGYKHCPGNTPECKSEDLLT 277
+ND E +L+CIIC E + A C FC CIN + + CP + KS+ +
Sbjct: 393 MNDVLENELQCIICSEYFIEAVTLNCAHSFCSYCINEWMKRKIE-CPICRKDIKSK-TYS 450
Query: 276 ISFDPIINKRISNLIARCPHER 211
+ D INK ++NL + R
Sbjct: 451 LVLDNCINKMVNNLSSEVKERR 472
>sp|P39429|TRAF2_MOUSE TNF receptor-associated factor 2
Length = 501
Score = 42.0 bits (97), Expect = 9e-04
Identities = 29/104 (27%), Positives = 47/104 (45%), Gaps = 7/104 (6%)
Frame = -2
Query: 426 CIICKEILVNAQQSLCGCHFCLVCINNYKNNGYKHCPGNTPECKSEDLLTI-----SF-D 265
C CK IL Q+ CG +C C+ + ++G ++C E E+ ++I +F D
Sbjct: 34 CSACKNILRRPFQAQCGHRYCSFCLTSILSSGPQNCAACVYEGLYEEGISILESSSAFPD 93
Query: 264 PIINKRISNLIARCPHERCFHECRLIEMED-HMRFCSTGNVNCP 136
+ + +L A CP++ C + L E E H C CP
Sbjct: 94 NAARREVESLPAVCPNDGCTWKGTLKEYESCHEGLCPFLLTECP 137
Score = 37.4 bits (85), Expect = 0.021
Identities = 18/47 (38%), Positives = 26/47 (55%)
Frame = -2
Query: 180 EDHMRFCSTGNVNCPFNNVGCEHEEMNIDKLNDHMLLEIVNHNKILI 40
+DH+R CS V C F+ VGC E + + L DH L + H +L+
Sbjct: 203 QDHVRACSKCRVLCRFHTVGCS-EMVETENLQDHELQRLREHLALLL 248
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 55,478,072
Number of Sequences: 369166
Number of extensions: 1117228
Number of successful extensions: 3013
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2907
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3004
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3354254700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)