Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_A20
(373 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P37167|ACTP_ACACA Actophorin 53 2e-07
sp|Q9LQ81|ADFX_ARATH Actin-depolymerizing factor like At1g0... 52 6e-07
sp|P18359|DEST_CHICK Destrin (Actin-depolymerizing factor) ... 51 9e-07
sp|Q39250|ADF1_ARATH Actin-depolymerizing factor 1 (ADF-1) ... 50 2e-06
sp|Q9FVI1|ADF2_PETHY Actin-depolymerizing factor 2 (ADF 2) 49 3e-06
sp|Q39251|ADF2_ARATH Actin-depolymerizing factor 2 (ADF-2) ... 49 4e-06
sp|Q43694|ADF2_MAIZE Actin-depolymerizing factor 2 (ADF 2) ... 49 4e-06
sp|P30174|ADF_BRANA Actin depolymerizing factor (ADF) 49 5e-06
sp|P23528|COF1_HUMAN Cofilin-1 (Cofilin, non-muscle isoform... 49 5e-06
sp|P45592|COF1_RAT Cofilin-1 (Cofilin, non-muscle isoform) 49 5e-06
>sp|P37167|ACTP_ACACA Actophorin
Length = 138
Score = 53.1 bits (126), Expect = 2e-07
Identities = 27/92 (29%), Positives = 47/92 (51%), Gaps = 8/92 (8%)
Frame = +2
Query: 17 YEKFKQHIIETYAQEGCYALYDYE--------GTLAFISWIPETMKINSRMIMAASKSEI 172
YE FK + E + YA++DYE + FI W P++ I S+M+ ++K I
Sbjct: 49 YEDFKSQLPERDCR---YAIFDYEFQVDGGQRNKITFILWAPDSAPIKSKMMYTSTKDSI 105
Query: 173 ATRMVGVKAKIEANSEDEIDESVIKEKTASKV 268
++VG++ +++A EI E + E+ V
Sbjct: 106 KKKLVGIQVEVQATDAAEISEDAVSERAKKDV 137
>sp|Q9LQ81|ADFX_ARATH Actin-depolymerizing factor like At1g01750 (ADF-like)
Length = 140
Score = 51.6 bits (122), Expect = 6e-07
Identities = 26/90 (28%), Positives = 46/90 (51%), Gaps = 9/90 (10%)
Frame = +2
Query: 5 LGTPYEKFKQHIIETYAQEGCYALYDYEGT---------LAFISWIPETMKINSRMIMAA 157
LG P E ++ E YA+YDY+ T + FI+W P+T ++ S+M+ A+
Sbjct: 46 LGNPEETYEDFTRSIPEDECRYAVYDYDFTTPENCQKSKIFFIAWSPDTSRVRSKMLYAS 105
Query: 158 SKSEIATRMVGVKAKIEANSEDEIDESVIK 247
SK + G++ +++A E+ +IK
Sbjct: 106 SKDRFKRELDGIQVELQATDPSEMSLDIIK 135
>sp|P18359|DEST_CHICK Destrin (Actin-depolymerizing factor) (ADF)
Length = 165
Score = 50.8 bits (120), Expect = 9e-07
Identities = 29/93 (31%), Positives = 47/93 (50%), Gaps = 9/93 (9%)
Frame = +2
Query: 2 DLGTPYEKFKQHIIETYAQEGC-YALYDY--------EGTLAFISWIPETMKINSRMIMA 154
D+G +H +E ++ C YALYD + L F W PE + S+MI A
Sbjct: 59 DVGVTVTDPFKHFVEMLPEKDCRYALYDASFETKESKKEELMFFLWAPEQAPLKSKMIYA 118
Query: 155 ASKSEIATRMVGVKAKIEANSEDEIDESVIKEK 253
+SK I + G+K + +AN ++++ + I EK
Sbjct: 119 SSKDAIKKKFQGIKHECQANGPEDLNRACIAEK 151
>sp|Q39250|ADF1_ARATH Actin-depolymerizing factor 1 (ADF-1) (AtADF1)
Length = 139
Score = 49.7 bits (117), Expect = 2e-06
Identities = 25/92 (27%), Positives = 47/92 (51%), Gaps = 9/92 (9%)
Frame = +2
Query: 5 LGTPYEKFKQHIIETYAQEGCYALYDYEGTLA---------FISWIPETMKINSRMIMAA 157
+G P + +++ A E YA+YD++ A FI+W P+ K+ S+MI A+
Sbjct: 46 VGQPIQTYEEFAACLPADECRYAIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYAS 105
Query: 158 SKSEIATRMVGVKAKIEANSEDEIDESVIKEK 253
SK + G++ +++A E+D V + +
Sbjct: 106 SKDRFKRELDGIQVELQATDPTEMDLDVFRSR 137
>sp|Q9FVI1|ADF2_PETHY Actin-depolymerizing factor 2 (ADF 2)
Length = 143
Score = 49.3 bits (116), Expect = 3e-06
Identities = 25/92 (27%), Positives = 46/92 (50%), Gaps = 9/92 (9%)
Frame = +2
Query: 5 LGTPYEKFKQHIIETYAQEGCYALYDYE---------GTLAFISWIPETMKINSRMIMAA 157
LG P E ++ A E YA+YD++ + FI+W P+T ++ S+MI A+
Sbjct: 46 LGEPTESYEDFTAGLPADECRYAVYDFDFMTKENHQKSRIFFIAWSPDTARVRSKMIYAS 105
Query: 158 SKSEIATRMVGVKAKIEANSEDEIDESVIKEK 253
SK + G++ +++A E+ V + +
Sbjct: 106 SKDRFKRELDGIQVELQATDPTEMGLDVFRSR 137
>sp|Q39251|ADF2_ARATH Actin-depolymerizing factor 2 (ADF-2) (AtADF2)
Length = 137
Score = 48.9 bits (115), Expect = 4e-06
Identities = 25/93 (26%), Positives = 44/93 (47%), Gaps = 9/93 (9%)
Frame = +2
Query: 5 LGTPYEKFKQHIIETYAQEGCYALYDYEGTLA---------FISWIPETMKINSRMIMAA 157
LG P + + A + Y +YD++ A FI+W P+T K+ +MI A+
Sbjct: 44 LGEPEQSYDDFAASLPADDCRYCIYDFDFVTAENCQKSKIFFIAWSPDTAKVRDKMIYAS 103
Query: 158 SKSEIATRMVGVKAKIEANSEDEIDESVIKEKT 256
SK + G++ +++A E+ V K +T
Sbjct: 104 SKDRFKRELDGIQVELQATDPTEMGLDVFKSRT 136
>sp|Q43694|ADF2_MAIZE Actin-depolymerizing factor 2 (ADF 2) (ZmABP2) (ZmADF2)
Length = 139
Score = 48.9 bits (115), Expect = 4e-06
Identities = 26/93 (27%), Positives = 45/93 (48%), Gaps = 9/93 (9%)
Frame = +2
Query: 5 LGTPYEKFKQHIIETYAQEGCYALYDYEGT---------LAFISWIPETMKINSRMIMAA 157
LG P + + A E YA+YD + T + F SW P+T + S+M+ A+
Sbjct: 46 LGEPNQGYGDFTDSLPADECRYAIYDLDFTTVENCQKSKIFFFSWSPDTARTRSKMLYAS 105
Query: 158 SKSEIATRMVGVKAKIEANSEDEIDESVIKEKT 256
SK + G++ +I+A E+ ++K +T
Sbjct: 106 SKDRFRRELDGIQCEIQATDPSEMSLDIVKSRT 138
>sp|P30174|ADF_BRANA Actin depolymerizing factor (ADF)
Length = 126
Score = 48.5 bits (114), Expect = 5e-06
Identities = 23/92 (25%), Positives = 46/92 (50%), Gaps = 9/92 (9%)
Frame = +2
Query: 5 LGTPYEKFKQHIIETYAQEGCYALYDYEGT---------LAFISWIPETMKINSRMIMAA 157
LG P E + A E YA++D++ T + FI+W P++ ++ +M+ A+
Sbjct: 33 LGNPQETYDDFTASLPADECRYAVFDFDFTTNENCQKSKIFFIAWSPDSSRVRMKMVYAS 92
Query: 158 SKSEIATRMVGVKAKIEANSEDEIDESVIKEK 253
SK + G++ +++A E+ +IK +
Sbjct: 93 SKDRFKRELDGIQVELQATDPSEMSFDIIKSR 124
>sp|P23528|COF1_HUMAN Cofilin-1 (Cofilin, non-muscle isoform) (18 kDa phosphoprotein)
(p18)
Length = 166
Score = 48.5 bits (114), Expect = 5e-06
Identities = 32/90 (35%), Positives = 48/90 (53%), Gaps = 10/90 (11%)
Frame = +2
Query: 14 PYEKFKQHIIETYAQEGC-YALYD--YEGT------LAFISWIPETMKINSRMIMAASKS 166
PY F ++ + C YALYD YE L FI W PE+ + S+MI A+SK
Sbjct: 67 PYATF----VKMLPDKDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKD 122
Query: 167 EIATRMVGVKAKIEANSEDEI-DESVIKEK 253
I ++ G+K +++AN +E+ D + EK
Sbjct: 123 AIKKKLTGIKHELQANCYEEVKDRCTLAEK 152
>sp|P45592|COF1_RAT Cofilin-1 (Cofilin, non-muscle isoform)
Length = 166
Score = 48.5 bits (114), Expect = 5e-06
Identities = 32/90 (35%), Positives = 48/90 (53%), Gaps = 10/90 (11%)
Frame = +2
Query: 14 PYEKFKQHIIETYAQEGC-YALYD--YEGT------LAFISWIPETMKINSRMIMAASKS 166
PY F ++ + C YALYD YE L FI W PE+ + S+MI A+SK
Sbjct: 67 PYTTF----VKMLPDKDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKD 122
Query: 167 EIATRMVGVKAKIEANSEDEI-DESVIKEK 253
I ++ G+K +++AN +E+ D + EK
Sbjct: 123 AIKKKLTGIKHELQANCYEEVKDRCTLAEK 152
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 36,703,680
Number of Sequences: 369166
Number of extensions: 545355
Number of successful extensions: 1359
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1341
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1356
length of database: 68,354,980
effective HSP length: 90
effective length of database: 51,728,830
effective search space used: 1707051390
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)