Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_A15
(649 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9BW30|CG38_HUMAN Protein CGI-38 90 6e-18
sp|Q9CRB6|CG38_MOUSE Protein CGI-38 homolog 88 2e-17
sp|P59282|P25B_HUMAN Protein p25-beta 87 5e-17
sp|Q7TQD2|P25A_MOUSE Tubulin polymerization-promoting prote... 84 2e-16
sp|O94811|P25A_HUMAN Tubulin polymerization-promoting prote... 81 3e-15
sp|Q27957|P25A_BOVIN Tubulin polymerization-promoting prote... 79 8e-15
sp|P91127|YBYK_CAEEL Hypothetical protein C32E8.3 in chromo... 67 5e-11
sp|Q9VV43|Y4893_DROME Hypothetical protein CG4893 67 5e-11
sp|Q4L5U2|TRMD_STAHJ tRNA (Guanine-N(1)-)-methyltransferase... 33 0.83
sp|P16342|R1AB_CVMA5 Replicase polyprotein 1ab (pp1ab) (ORF... 32 1.9
>sp|Q9BW30|CG38_HUMAN Protein CGI-38
Length = 176
Score = 89.7 bits (221), Expect = 6e-18
Identities = 56/148 (37%), Positives = 76/148 (51%), Gaps = 1/148 (0%)
Frame = +2
Query: 71 KTLKKICTDCKIYC-KGFDQNRCDIQFRKHIGNAKKDVDYNDFVRFVEGPMAEAYMSARN 247
K K+C DCK+ K DI F K G + + ++Y +F + +E + +
Sbjct: 33 KNWAKLCKDCKVADGKSVTGTDVDIVFSKVKGKSARVINYEEFKKALEELATKRFKGKSK 92
Query: 248 LAYEDAVQELKTKICQGSPAGHGTTGVSADAATARLTDVKGYTGAHKERFDAETGKGKGI 427
DA+ +L + PA G T A RLTD YTG+HKERFD E+GKGKGI
Sbjct: 93 EEAFDAICQL---VAGKEPANVGVTKAKTGGAVDRLTDTSRYTGSHKERFD-ESGKGKGI 148
Query: 428 DGREYLAGENAQQGYVGGYKGAGTYDKK 511
GR+ + ++ GYV YK AGTYD K
Sbjct: 149 AGRQDILDDS---GYVSAYKNAGTYDAK 173
>sp|Q9CRB6|CG38_MOUSE Protein CGI-38 homolog
Length = 176
Score = 87.8 bits (216), Expect = 2e-17
Identities = 56/148 (37%), Positives = 75/148 (50%), Gaps = 1/148 (0%)
Frame = +2
Query: 71 KTLKKICTDCKIYC-KGFDQNRCDIQFRKHIGNAKKDVDYNDFVRFVEGPMAEAYMSARN 247
K K+C DCK+ K DI F K + + ++Y +F + +E + +
Sbjct: 33 KNWAKLCKDCKVADGKAVTGTDVDIVFSKVKAKSARVINYEEFKKALEELATKRFKGKSK 92
Query: 248 LAYEDAVQELKTKICQGSPAGHGTTGVSADAATARLTDVKGYTGAHKERFDAETGKGKGI 427
DA+ +L I PA G T A RLTD YTG+HKERFD E+GKGKGI
Sbjct: 93 EEAFDAICQL---IAGKEPANIGVTKAKTGGAVDRLTDTSKYTGSHKERFD-ESGKGKGI 148
Query: 428 DGREYLAGENAQQGYVGGYKGAGTYDKK 511
GR+ + ++ GYV YK AGTYD K
Sbjct: 149 AGRQDILDDS---GYVSAYKNAGTYDAK 173
>sp|P59282|P25B_HUMAN Protein p25-beta
Length = 170
Score = 86.7 bits (213), Expect = 5e-17
Identities = 63/166 (37%), Positives = 81/166 (48%), Gaps = 2/166 (1%)
Frame = +2
Query: 20 FAAFCDAVKKGSTTCTDKTLKKICTDCKIYC-KGFDQNRCDIQFRKHIGNAKKDVDYNDF 196
FAAF ++ G T +K K+C DC I K DI F K + + + F
Sbjct: 12 FAAFGESSSSG-TEMNNKNFSKLCKDCGIMDGKTVTSTDVDIVFSKVKAKNARTITFQQF 70
Query: 197 VRFVEGPMAEAYMSARNLAYEDAVQELKTKICQGS-PAGHGTTGVSADAATARLTDVKGY 373
V+ + + ++ D V E + +G PA G T + A RLTD Y
Sbjct: 71 KEAVK-ELGQKRFKGKS---PDEVLENIYGLMEGKDPATTGATKATTVGAVDRLTDTSKY 126
Query: 374 TGAHKERFDAETGKGKGIDGREYLAGENAQQGYVGGYKGAGTYDKK 511
TG HKE FD E+GKGKGI GRE + GYV GYKG+GTYDKK
Sbjct: 127 TGTHKELFD-ESGKGKGIAGREEMTDNT---GYVSGYKGSGTYDKK 168
>sp|Q7TQD2|P25A_MOUSE Tubulin polymerization-promoting protein (TPPP)
Length = 218
Score = 84.3 bits (207), Expect = 2e-16
Identities = 61/175 (34%), Positives = 87/175 (49%), Gaps = 6/175 (3%)
Frame = +2
Query: 5 DLRAAFAAFCDAVKKGSTTCTDKTL-----KKICTDCKIYC-KGFDQNRCDIQFRKHIGN 166
+L A AF G T T K + K+C DC + K DI F K G
Sbjct: 46 ELSALEEAFRRFAVHGDTRATGKEMHGKNWSKLCKDCHVIDGKNVTVTDVDIVFSKIKGK 105
Query: 167 AKKDVDYNDFVRFVEGPMAEAYMSARNLAYEDAVQELKTKICQGSPAGHGTTGVSADAAT 346
+ + + + F +E + + ++ + E+AV+E+ I +P G T +
Sbjct: 106 SCRTITFEQFQEALEELAKKRF---KDKSSEEAVREVHRLIEGRAPVISGVTKAVSSPTV 162
Query: 347 ARLTDVKGYTGAHKERFDAETGKGKGIDGREYLAGENAQQGYVGGYKGAGTYDKK 511
+RLTD +TG+HKERFD ++GKGKG GR L E+ GYV GYK AGTYD+K
Sbjct: 163 SRLTDTSKFTGSHKERFD-QSGKGKGKAGRVDLVDES---GYVPGYKHAGTYDQK 213
>sp|O94811|P25A_HUMAN Tubulin polymerization-promoting protein (TPPP) (25 kDa
brain-specific protein) (p25-alpha) (p24) (p25)
Length = 219
Score = 80.9 bits (198), Expect = 3e-15
Identities = 53/148 (35%), Positives = 78/148 (52%), Gaps = 1/148 (0%)
Frame = +2
Query: 71 KTLKKICTDCKIYC-KGFDQNRCDIQFRKHIGNAKKDVDYNDFVRFVEGPMAEAYMSARN 247
K K+C DC++ + DI F K G + + + + F +E + + ++
Sbjct: 74 KNWSKLCKDCQVIDGRNVTVTDVDIVFSKIKGKSCRTITFEQFQEALEELAKKRF---KD 130
Query: 248 LAYEDAVQELKTKICQGSPAGHGTTGVSADAATARLTDVKGYTGAHKERFDAETGKGKGI 427
+ E+AV+E+ I +P G T + +RLTD +TG+HKERFD +GKGKG
Sbjct: 131 KSSEEAVREVHRLIEGKAPIISGVTKAISSPTVSRLTDTTKFTGSHKERFD-PSGKGKGK 189
Query: 428 DGREYLAGENAQQGYVGGYKGAGTYDKK 511
GR L E+ GYV GYK AGTYD+K
Sbjct: 190 AGRVDLVDES---GYVSGYKHAGTYDQK 214
>sp|Q27957|P25A_BOVIN Tubulin polymerization-promoting protein (TPPP) (25 kDa
brain-specific protein) (p25-alpha)
Length = 218
Score = 79.3 bits (194), Expect = 8e-15
Identities = 52/148 (35%), Positives = 78/148 (52%), Gaps = 1/148 (0%)
Frame = +2
Query: 71 KTLKKICTDCKIYC-KGFDQNRCDIQFRKHIGNAKKDVDYNDFVRFVEGPMAEAYMSARN 247
K K+C DC++ + DI F K G + + + + F +E + + ++
Sbjct: 73 KNWSKLCRDCQVIDGRSVTVTDVDIVFSKIKGKSCRTITFEQFKEALEELAKKRF---KD 129
Query: 248 LAYEDAVQELKTKICQGSPAGHGTTGVSADAATARLTDVKGYTGAHKERFDAETGKGKGI 427
+ E+AV+E+ I +P G T + +RLTD +TG+HKERFD +G+GKG
Sbjct: 130 KSAEEAVREVHKLIEGKAPIISGVTKAISSPTVSRLTDTSKFTGSHKERFD-PSGRGKGR 188
Query: 428 DGREYLAGENAQQGYVGGYKGAGTYDKK 511
GR L E+ GYV GYK AGTYD+K
Sbjct: 189 AGRVDLVDES---GYVPGYKHAGTYDQK 213
>sp|P91127|YBYK_CAEEL Hypothetical protein C32E8.3 in chromosome I
Length = 180
Score = 66.6 bits (161), Expect = 5e-11
Identities = 57/170 (33%), Positives = 74/170 (43%), Gaps = 2/170 (1%)
Frame = +2
Query: 5 DLRAAFAAFCDAVKKGSTTCTDKTLKKICTDCKIYC-KGFDQNRCDIQFRKHIGNAKKDV 181
D++ + AF +T T K K D + K I F K G KK
Sbjct: 13 DVKKRWDAFTKFGAATATEMTGKNFDKWLKDAGVLDNKAITGTMTGIAFSKVTGPKKKAT 72
Query: 182 DYNDFVRFVEGPMAEAYMSARNLAYEDAVQELKTKICQ-GSPAGHGTTGVSADAATARLT 358
D + V +AE +D + + K+ + +P+ G +A +RLT
Sbjct: 73 F--DETKKVLAFVAEDRARQSKKPIQDELDAITEKLAKLEAPSVGGAAKANAAGVYSRLT 130
Query: 359 DVKGYTGAHKERFDAETGKGKGIDGREYLAGENAQQGYVGGYKGAGTYDK 508
D YTGAHKERFDAE GKGKG GR A GYVG YK +YDK
Sbjct: 131 DHTKYTGAHKERFDAE-GKGKGKSGR---ADTTENTGYVGAYKNKDSYDK 176
>sp|Q9VV43|Y4893_DROME Hypothetical protein CG4893
Length = 192
Score = 66.6 bits (161), Expect = 5e-11
Identities = 57/167 (34%), Positives = 79/167 (47%), Gaps = 4/167 (2%)
Frame = +2
Query: 17 AFAAFCDAVKKG---STTCTDKTLKKICTDCKIYCKGFDQNRCDIQFRKHIGNAKKDVDY 187
AF+ F D+ G + + +DK +K+ K+ K I F+K DY
Sbjct: 43 AFSKFGDSKSDGKLITLSQSDKWMKQ----AKVIDKKITTTDTGIHFKKFKAMKISLSDY 98
Query: 188 NDFVRFVEGPMAEAYMSARNLAYEDAVQELKTKICQ-GSPAGHGTTGVSADAATARLTDV 364
N F+ + A+ E + E+K K+ G+P + A AA RLTD
Sbjct: 99 NKFLDDL----------AKTKKVE--LSEIKQKLASCGAPGVVSVSAGKAAAAVDRLTDT 146
Query: 365 KGYTGAHKERFDAETGKGKGIDGREYLAGENAQQGYVGGYKGAGTYD 505
YTG+HKERFDA +GKGKGI GR + + GYV GY+ TYD
Sbjct: 147 SKYTGSHKERFDA-SGKGKGIAGRRNVVDGS---GYVSGYQHKDTYD 189
>sp|Q4L5U2|TRMD_STAHJ tRNA (Guanine-N(1)-)-methyltransferase (M1G-methyltransferase)
(tRNA [GM37] methyltransferase)
Length = 245
Score = 32.7 bits (73), Expect = 0.83
Identities = 21/48 (43%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
Frame = -3
Query: 362 HLSNEQLPHQQKL-RLYHGRQDYLDRF*FSILVLRLHKPNFLHSYKLQ 222
H + EQ H+QKL R YH R D LDR+ L F+ SYK Q
Sbjct: 199 HANIEQWRHEQKLIRTYHKRPDLLDRY-----PLTEDDEKFIESYKKQ 241
>sp|P16342|R1AB_CVMA5 Replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) [Includes:
Replicase polyprotein 1a (pp1a) (ORF1a)] [Contains: p28;
p65; p210 (Papain-like proteinases 1/2)
(PL1-PRO/PL2-PRO); Peptide HD2 (p44); 3C-like proteinase
(3CL-PRO) (3CLp) (M-PRO) (p27); Unknown protein 1; p10;
p22; p12; Growth factor-like peptide (GFL) (p15);
RNA-directed RNA polymerase (RdRp) (Pol) (p100); Helicase
(Hel) (p67); Unknown protein 2; p35; Unknown protein 3]
Length = 7176
Score = 31.6 bits (70), Expect = 1.9
Identities = 13/45 (28%), Positives = 25/45 (55%)
Frame = -1
Query: 343 CRISRNSGCTMAGRTTLTDFSFQFLYCVFISQISCTHISFSHWSF 209
CR++ + C M+GR +LT S+Q C + ++ + + +SF
Sbjct: 3392 CRVTSSDFCVMSGRMSLTVMSYQMQGCQLVLTVTLQNPNTPKYSF 3436
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 65,846,066
Number of Sequences: 369166
Number of extensions: 1283638
Number of successful extensions: 4245
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4091
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4228
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 5316264630
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)