Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_A11
(598 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9DBI0|TMPS6_MOUSE Transmembrane protease, serine 6 (Mat... 99 8e-21
sp|P26262|KLKB1_MOUSE Plasma kallikrein precursor (Plasma p... 94 3e-19
sp|Q8IU80|TMPS6_HUMAN Transmembrane protease, serine 6 (Mat... 91 2e-18
sp|P14272|KLKB1_RAT Plasma kallikrein precursor (Plasma pre... 91 3e-18
sp|P03952|KLKB1_HUMAN Plasma kallikrein precursor (Plasma p... 87 4e-17
sp|P19221|THRB_MOUSE Prothrombin precursor (Coagulation fac... 84 4e-16
sp|Q5QSK2|DESC4_RAT Serine protease DESC4 precursor [Contai... 84 4e-16
sp|Q5K2P8|POLS2_MOUSE Polyserase-2 precursor (Polyserine pr... 82 8e-16
sp|Q5U405|TMPSD_MOUSE Transmembrane protease, serine 13 (Mo... 82 8e-16
sp|P18292|THRB_RAT Prothrombin precursor (Coagulation facto... 82 1e-15
>sp|Q9DBI0|TMPS6_MOUSE Transmembrane protease, serine 6 (Matriptase-2)
Length = 799
Score = 99.0 bits (245), Expect = 8e-21
Identities = 61/175 (34%), Positives = 95/175 (54%), Gaps = 2/175 (1%)
Frame = +2
Query: 17 SNLYDIALLKLDKPVIPSKVNINFPCVPQVKNYTLDKSAKCYAIGWGNIEQNEPYNIWSF 196
S+ YD+ALL+LD PV+ S + C+P +++ + C+ GWG + P
Sbjct: 652 SHDYDVALLQLDHPVVYS-ATVRPVCLP-ARSHFFEPGQHCWITGWGAQREGGPV----- 704
Query: 197 EFDFVLERWRKNKSQKNSNILQEVEIPLVNFELCQKRYRIFNLNKEIHICAGSRR--KDT 370
SN LQ+V++ LV +LC + YR + ++ + +CAG R+ KD
Sbjct: 705 -----------------SNTLQKVDVQLVPQDLCSEAYR-YQVSPRM-LCAGYRKGKKDA 745
Query: 371 CRGDSGGGLFCLDPSTNRWQLYGITNFGSARGCGTSYGVYTRVPEVSNWIKEIIS 535
C+GDSGG L C +PS RW L G+ ++G G +GVYTRV V NWI+++++
Sbjct: 746 CQGDSGGPLVCREPS-GRWFLAGLVSWGLGCGRPNFFGVYTRVTRVINWIQQVLT 799
>sp|P26262|KLKB1_MOUSE Plasma kallikrein precursor (Plasma prekallikrein) (Kininogenin)
(Fletcher factor) [Contains: Plasma kallikrein heavy
chain; Plasma kallikrein light chain]
Length = 638
Score = 93.6 bits (231), Expect = 3e-19
Identities = 64/173 (36%), Positives = 86/173 (49%), Gaps = 2/173 (1%)
Frame = +2
Query: 14 SSNLYDIALLKLDKPVIPSKVNINFPCVPQVKNYTLDKSAKCYAIGWGNIEQNEPYNIWS 193
S YDIAL+KL P+ ++ C+P K T C+ GWG ++
Sbjct: 478 SEGNYDIALIKLQTPLNYTEFQKPI-CLPS-KADTNTIYTNCWVTGWGYTKEQG------ 529
Query: 194 FEFDFVLERWRKNKSQKNSNILQEVEIPLVNFELCQKRYRIFNLNKEIHICAGSRR--KD 367
+ NILQ+ IPLV E CQK+YR + +NK++ ICAG + D
Sbjct: 530 ----------------ETQNILQKATIPLVPNEECQKKYRDYVINKQM-ICAGYKEGGTD 572
Query: 368 TCRGDSGGGLFCLDPSTNRWQLYGITNFGSARGCGTSYGVYTRVPEVSNWIKE 526
C+GDSGG L C + RWQL GIT++G G GVYT+V E +WI E
Sbjct: 573 ACKGDSGGPLVC--KHSGRWQLVGITSWGEGCGRKDQPGVYTKVSEYMDWILE 623
>sp|Q8IU80|TMPS6_HUMAN Transmembrane protease, serine 6 (Matriptase-2)
Length = 802
Score = 91.3 bits (225), Expect = 2e-18
Identities = 57/175 (32%), Positives = 93/175 (53%), Gaps = 2/175 (1%)
Frame = +2
Query: 17 SNLYDIALLKLDKPVIPSKVNINFPCVPQVKNYTLDKSAKCYAIGWGNIEQNEPYNIWSF 196
S+ YD+ALL+LD PV+ S + C+P +++ + C+ GWG + + P
Sbjct: 655 SHDYDVALLQLDHPVVRSAA-VRPVCLP-ARSHFFEPGLHCWITGWGALREGGPI----- 707
Query: 197 EFDFVLERWRKNKSQKNSNILQEVEIPLVNFELCQKRYRIFNLNKEIHICAGSRR--KDT 370
SN LQ+V++ L+ +LC + YR + + + +CAG R+ KD
Sbjct: 708 -----------------SNALQKVDVQLIPQDLCSEVYR-YQVTPRM-LCAGYRKGKKDA 748
Query: 371 CRGDSGGGLFCLDPSTNRWQLYGITNFGSARGCGTSYGVYTRVPEVSNWIKEIIS 535
C+GDSGG L C S RW L G+ ++G G +GVYTR+ V +WI+++++
Sbjct: 749 CQGDSGGPLVCKALS-GRWFLAGLVSWGLGCGRPNYFGVYTRITGVISWIQQVVT 802
>sp|P14272|KLKB1_RAT Plasma kallikrein precursor (Plasma prekallikrein) (Kininogenin)
(Fletcher factor) [Contains: Plasma kallikrein heavy
chain; Plasma kallikrein light chain]
Length = 638
Score = 90.5 bits (223), Expect = 3e-18
Identities = 64/178 (35%), Positives = 86/178 (48%), Gaps = 2/178 (1%)
Frame = +2
Query: 14 SSNLYDIALLKLDKPVIPSKVNINFPCVPQVKNYTLDKSAKCYAIGWGNIEQNEPYNIWS 193
S YDIAL+KL P+ ++ C+P K T C+ GWG ++
Sbjct: 478 SEGSYDIALIKLQTPLNYTEFQKPI-CLPS-KADTNTIYTNCWVTGWGYTKERG------ 529
Query: 194 FEFDFVLERWRKNKSQKNSNILQEVEIPLVNFELCQKRYRIFNLNKEIHICAGSRRK--D 367
+ NILQ+ IPLV E CQK+YR + + K++ ICAG + D
Sbjct: 530 ----------------ETQNILQKATIPLVPNEECQKKYRDYVITKQM-ICAGYKEGGID 572
Query: 368 TCRGDSGGGLFCLDPSTNRWQLYGITNFGSARGCGTSYGVYTRVPEVSNWIKEIISKS 541
C+GDSGG L C + RWQL GIT++G GVYT+V E +WI E I S
Sbjct: 573 ACKGDSGGPLVC--KHSGRWQLVGITSWGEGCARKEQPGVYTKVAEYIDWILEKIQSS 628
>sp|P03952|KLKB1_HUMAN Plasma kallikrein precursor (Plasma prekallikrein) (Kininogenin)
(Fletcher factor) [Contains: Plasma kallikrein heavy
chain; Plasma kallikrein light chain]
Length = 638
Score = 86.7 bits (213), Expect = 4e-17
Identities = 61/173 (35%), Positives = 84/173 (48%), Gaps = 2/173 (1%)
Frame = +2
Query: 14 SSNLYDIALLKLDKPVIPSKVNINFPCVPQVKNYTLDKSAKCYAIGWGNIEQNEPYNIWS 193
S +DIAL+KL P+ ++ C+P K T C+ GWG
Sbjct: 478 SEGNHDIALIKLQAPLNYTEFQKPI-CLPS-KGDTSTIYTNCWVTGWG------------ 523
Query: 194 FEFDFVLERWRKNKSQKNSNILQEVEIPLVNFELCQKRYRIFNLNKEIHICAGSRR--KD 367
+ K K + NILQ+V IPLV E CQKRY+ + + + + +CAG + KD
Sbjct: 524 ---------FSKEKGEIQ-NILQKVNIPLVTNEECQKRYQDYKITQRM-VCAGYKEGGKD 572
Query: 368 TCRGDSGGGLFCLDPSTNRWQLYGITNFGSARGCGTSYGVYTRVPEVSNWIKE 526
C+GDSGG L C W+L GIT++G GVYT+V E +WI E
Sbjct: 573 ACKGDSGGPLVC--KHNGMWRLVGITSWGEGCARREQPGVYTKVAEYMDWILE 623
>sp|P19221|THRB_MOUSE Prothrombin precursor (Coagulation factor II) [Contains: Activation
peptide fragment 1; Activation peptide fragment 2;
Thrombin light chain; Thrombin heavy chain]
Length = 618
Score = 83.6 bits (205), Expect = 4e-16
Identities = 61/187 (32%), Positives = 85/187 (45%), Gaps = 8/187 (4%)
Frame = +2
Query: 2 RADYSSNL-YDIALLKLDKPVIPSKVNINFPCVPQVKNYT--LDKSAKCYAIGWGNIEQN 172
R ++ NL DIALLKL KPV P I+ C+P + T L K GWGN+
Sbjct: 449 RYNWRENLDRDIALLKLKKPV-PFSDYIHPVCLPDKQTVTSLLRAGYKGRVTGWGNLR-- 505
Query: 173 EPYNIWSFEFDFVLERWRKNKSQKNSNILQEVEIPLVNFELCQKRYRIFNLNKEIHICAG 352
E W N ++ ++LQ V +P+V +C+ RI + CAG
Sbjct: 506 --------------ETWTTNINEIQPSVLQVVNLPIVERPVCKASTRIRITDNMF--CAG 549
Query: 353 -----SRRKDTCRGDSGGGLFCLDPSTNRWQLYGITNFGSARGCGTSYGVYTRVPEVSNW 517
++R D C GDSGG P NRW GI ++G YG YT V + W
Sbjct: 550 FKVNDTKRGDACEGDSGGPFVMKSPFNNRWYQMGIVSWGEGCDRKGKYGFYTHVFRLKRW 609
Query: 518 IKEIISK 538
I+++I +
Sbjct: 610 IQKVIDQ 616
>sp|Q5QSK2|DESC4_RAT Serine protease DESC4 precursor [Contains: Serine protease DESC4
non-catalytic chain; Serine protease DESC4 catalytic
chain]
Length = 417
Score = 83.6 bits (205), Expect = 4e-16
Identities = 57/167 (34%), Positives = 81/167 (48%), Gaps = 2/167 (1%)
Frame = +2
Query: 29 DIALLKLDKPVIPSKVNINFPCVPQVKNYTLDKSAKCYAIGWGNIEQNEPYNIWSFEFDF 208
DIA++KL PV+ S+ N+ C+P+ L KS K + GWG ++ N P+
Sbjct: 271 DIAVVKLSSPVLFSE-NLRTVCLPEATFQVLPKS-KVFVTGWGALKANGPF--------- 319
Query: 209 VLERWRKNKSQKNSNILQEVEIPLVNFELCQKRYRIFNLNKEIHICAG--SRRKDTCRGD 382
N LQEVEI +++ ++C + ICAG + + D C GD
Sbjct: 320 -------------PNSLQEVEIEIISNDVCNQVNVYGGAISSGMICAGFLTGKLDACEGD 366
Query: 383 SGGGLFCLDPSTNRWQLYGITNFGSARGCGTSYGVYTRVPEVSNWIK 523
SGG L D + N+W L GI ++G G G+YTRV NWIK
Sbjct: 367 SGGPLVISD-NRNKWYLLGIVSWGIDCGKENKPGIYTRVTHYRNWIK 412
>sp|Q5K2P8|POLS2_MOUSE Polyserase-2 precursor (Polyserine protease-2) (Protease serine 36)
Length = 849
Score = 82.4 bits (202), Expect = 8e-16
Identities = 60/179 (33%), Positives = 89/179 (49%), Gaps = 8/179 (4%)
Frame = +2
Query: 29 DIALLKLDKPVIPSKVNINFPCVPQVKNYTLDKSAKCYAIGWGNIEQNEPYNIWSFEFDF 208
D+ALL+L P ++ C+P+ + +A C+A GWG++++++P +
Sbjct: 139 DLALLRLASPAKLGP-SVKPVCLPRASHLFAHGTA-CWATGWGDVQESDPLPV------- 189
Query: 209 VLERWRKNKSQKNSNILQEVEIPLVNFELCQKRYRI---FNLNKEI---HICAG--SRRK 364
W +LQEVE+ L+ CQ Y FNL ++ +CAG R+
Sbjct: 190 ---PW----------VLQEVELKLLGETACQCLYSRPGPFNLTLQLLPGMLCAGYPEGRR 236
Query: 365 DTCRGDSGGGLFCLDPSTNRWQLYGITNFGSARGCGTSYGVYTRVPEVSNWIKEIISKS 541
DTC+GDSGG L C D RW L GIT+FG G GV+T V +WI+E + S
Sbjct: 237 DTCQGDSGGPLVCEDGG--RWFLAGITSFGFGCGRRNRPGVFTAVAHYESWIREHVMGS 293
>sp|Q5U405|TMPSD_MOUSE Transmembrane protease, serine 13 (Mosaic serine protease)
(Membrane-type mosaic serine protease)
Length = 543
Score = 82.4 bits (202), Expect = 8e-16
Identities = 54/167 (32%), Positives = 84/167 (50%), Gaps = 2/167 (1%)
Frame = +2
Query: 26 YDIALLKLDKPVIPSKVNINFPCVPQVKNYTLDKSAKCYAIGWGNIEQNEPYNIWSFEFD 205
YDIAL++L KP+ S +I+ C+P + T + C+ G+G ++ +
Sbjct: 389 YDIALIRLSKPLTLS-AHIHPACLP-MHGQTFGLNETCWITGFGKTKETD---------- 436
Query: 206 FVLERWRKNKSQKNSNILQEVEIPLVNFELCQKRYRIFNLNKEIHICAGSRR--KDTCRG 379
+K S L+EV++ L++F+ C + +CAG R +D+C+G
Sbjct: 437 -----------EKTSPFLREVQVNLIDFKKCNDYLVYDSYLTPRMMCAGDLRGGRDSCQG 485
Query: 380 DSGGGLFCLDPSTNRWQLYGITNFGSARGCGTSYGVYTRVPEVSNWI 520
DSGG L C NRW L G+T++G+ G GVYT+V EV WI
Sbjct: 486 DSGGPLVC--EQNNRWYLAGVTSWGTGCGQKNKPGVYTKVTEVLPWI 530
>sp|P18292|THRB_RAT Prothrombin precursor (Coagulation factor II) [Contains: Activation
peptide fragment 1; Activation peptide fragment 2;
Thrombin light chain; Thrombin heavy chain]
Length = 617
Score = 82.0 bits (201), Expect = 1e-15
Identities = 59/187 (31%), Positives = 85/187 (45%), Gaps = 8/187 (4%)
Frame = +2
Query: 2 RADYSSNL-YDIALLKLDKPVIPSKVNINFPCVPQVKNYT--LDKSAKCYAIGWGNIEQN 172
R ++ NL DIALLKL KPV P I+ C+P + T L K GWGN+
Sbjct: 448 RYNWRENLDRDIALLKLKKPV-PFSDYIHPVCLPDKQTVTSLLQAGYKGRVTGWGNLR-- 504
Query: 173 EPYNIWSFEFDFVLERWRKNKSQKNSNILQEVEIPLVNFELCQKRYRIFNLNKEIHICAG 352
E W N ++ ++LQ V +P+V +C+ RI + CAG
Sbjct: 505 --------------ETWTTNINEIQPSVLQVVNLPIVERPVCKASTRIRITDNMF--CAG 548
Query: 353 -----SRRKDTCRGDSGGGLFCLDPSTNRWQLYGITNFGSARGCGTSYGVYTRVPEVSNW 517
++R D C GDSGG P +RW GI ++G YG YT V + W
Sbjct: 549 FKVNDTKRGDACEGDSGGPFVMKSPYNHRWYQMGIVSWGEGCDRNGKYGFYTHVFRLKRW 608
Query: 518 IKEIISK 538
++++I +
Sbjct: 609 MQKVIDQ 615
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 71,932,205
Number of Sequences: 369166
Number of extensions: 1578118
Number of successful extensions: 5126
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4382
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4514
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4553075865
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)