Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_A01
(855 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q80ZK9|WDTC1_MOUSE WD and tetratricopeptide repeats prot... 162 1e-39
sp|Q8N5D0|WDTC1_HUMAN WD and tetratricopeptide repeats prot... 161 2e-39
sp|P07213|TOM70_YEAST Mitochondrial precursor proteins impo... 50 8e-06
sp|Q5U2X2|SPAG1_RAT Sperm-associated antigen 1 (Infertility... 49 2e-05
sp|Q9UNE7|STUB1_HUMAN STIP1 homology and U box-containing p... 48 3e-05
sp|Q9WUD1|STUB1_MOUSE STIP1 homology and U box-containing p... 48 4e-05
sp|P38825|YHR7_YEAST Hypothetical 71.9 kDa protein in ERP5-... 45 2e-04
sp|Q5ZHY5|STUB1_CHICK STIP1 homology and U box-containing p... 45 2e-04
sp|Q9QYI3|DNJC7_MOUSE DnaJ homolog subfamily C member 7 (Te... 45 2e-04
sp|Q8R3H9|TTC4_MOUSE Tetratricopeptide repeat protein 4 (TP... 43 0.001
>sp|Q80ZK9|WDTC1_MOUSE WD and tetratricopeptide repeats protein 1
Length = 677
Score = 162 bits (409), Expect = 1e-39
Identities = 101/292 (34%), Positives = 153/292 (52%), Gaps = 8/292 (2%)
Frame = +1
Query: 4 NNVLINLNSHIGSGCEVKCLAINPVRSDQLAVGSNDQYIRLYDRRKLSLKSYQNFDKKNK 183
+ VLI+L + G E KCL +NP ++ LAVG++ ++RLYD R + K
Sbjct: 171 SEVLIDLTEYCGPMVEAKCLTVNPQDNNCLAVGASGPFVRLYDIRMIH--------NHRK 222
Query: 184 GLYNKLSNGTTPLSPDENDMKSIPDDAVQYYVPSHIPWS--NQTDKYMQYTCTYLTFSPD 357
+ S G + K +PD A QYYV H+P + + TY+TFSP+
Sbjct: 223 SMRQSPSAGVHTFCDRQ---KPLPDGAAQYYVAGHLPVKLPDYNSRLRVLVATYVTFSPN 279
Query: 358 GNSLLVNMGSEQVYLYDVN-KSRPSFSFQSSLAKYVSTCNCDLLCDYSDARNLSNDIKSS 534
G LLVNMG EQVYL+D+ K RP V N + +SN + +
Sbjct: 280 GTELLVNMGGEQVYLFDLTYKQRPYTFLLPRKCHSVEVQNGKM-----STNGVSNGVSNG 334
Query: 535 INYTTNLSDVDKESG-----VEMLPHLNILKDQGNQMYKNSEFHSAITYYNIALQQGGNI 699
++ +N + + G VE+ P+L +K Q N+ + ++ AI Y+ A+Q+ +
Sbjct: 335 LHLHSNGFRLPESKGCISPQVELPPYLERVKQQANEAFACQQWTQAIQLYSQAVQKAPH- 393
Query: 700 HARFYANRAAAYISRNWAGDIYAALLDSIKALEIDYSHIKSHFRIAQCLFQL 855
+A Y NRAAAY+ R W GD Y AL D +KA+ ++ H+K+HFR+A+CLF+L
Sbjct: 394 NAMLYGNRAAAYMKRKWDGDHYDALRDCLKAISLNPCHLKAHFRLARCLFEL 445
>sp|Q8N5D0|WDTC1_HUMAN WD and tetratricopeptide repeats protein 1
Length = 677
Score = 161 bits (408), Expect = 2e-39
Identities = 98/291 (33%), Positives = 156/291 (53%), Gaps = 7/291 (2%)
Frame = +1
Query: 4 NNVLINLNSHIGSGCEVKCLAINPVRSDQLAVGSNDQYIRLYDRRKLSLKSYQNFDKKNK 183
+ VLI+L + G E KCL +NP ++ LAVG++ ++RLYD R + K
Sbjct: 171 SEVLIDLTEYCGQLVEAKCLTVNPQDNNCLAVGASGPFVRLYDIRMIH--------NHRK 222
Query: 184 GLYNKLSNGTTPLSPDENDMKSIPDDAVQYYVPSHIPWS--NQTDKYMQYTCTYLTFSPD 357
+ S G + K +PD A QYYV H+P + ++ TY+TFSP+
Sbjct: 223 SMKQSPSAGVHTFCDRQ---KPLPDGAAQYYVAGHLPVKLPDYNNRLRVLVATYVTFSPN 279
Query: 358 GNSLLVNMGSEQVYLYDVNKSRPSFSFQSSLAKYVSTCNCDLLCDYSDARNLSNDIKSSI 537
G LLVNMG EQVYL+D+ + ++F L + ++ +SN + + +
Sbjct: 280 GTELLVNMGGEQVYLFDLTYKQRPYTF---LLPRKCHSSGEVQNGKMSTNGVSNGVSNGL 336
Query: 538 NYTTNLSDVDKESG-----VEMLPHLNILKDQGNQMYKNSEFHSAITYYNIALQQGGNIH 702
+ +N + + G VE+ P+L +K Q N+ + ++ AI Y+ A+Q+ + +
Sbjct: 337 HLHSNGFRLPESRGHVSPQVELPPYLERVKQQANEAFACQQWTQAIQLYSKAVQRAPH-N 395
Query: 703 ARFYANRAAAYISRNWAGDIYAALLDSIKALEIDYSHIKSHFRIAQCLFQL 855
A Y NRAAAY+ R W GD Y AL D +KA+ ++ H+K+HFR+A+CLF+L
Sbjct: 396 AMLYGNRAAAYMKRKWDGDHYDALRDCLKAISLNPCHLKAHFRLARCLFEL 446
>sp|P07213|TOM70_YEAST Mitochondrial precursor proteins import receptor (70 kDa
mitochondrial outer membrane protein) (Translocase of
outer membrane TOM70)
Length = 617
Score = 50.1 bits (118), Expect = 8e-06
Identities = 36/110 (32%), Positives = 54/110 (49%), Gaps = 2/110 (1%)
Frame = +1
Query: 493 YSDARNLSNDIKSSINYTTNLSDVDKESGVEMLPHLNILKDQGNQMYKNSEFHSAITYYN 672
Y + N D + N+T D + LKD+GNQ ++N ++ AI YYN
Sbjct: 74 YPVSSNGEPDFSNKANFTAEEKD----------KYALALKDKGNQFFRNKKYDDAIKYYN 123
Query: 673 IALQQGGNIHARFYANRAAAYISRNWAGDIYAALLDSIKALEI--DYSHI 816
AL+ + FY+N +A Y+S GD+ + S KALE+ DYS +
Sbjct: 124 WALELKED--PVFYSNLSACYVS---VGDLKKVVEMSTKALELKPDYSKV 168
>sp|Q5U2X2|SPAG1_RAT Sperm-associated antigen 1 (Infertility-related sperm protein
Spag-1) (HSD-3.8)
Length = 893
Score = 48.5 bits (114), Expect = 2e-05
Identities = 28/76 (36%), Positives = 42/76 (55%)
Frame = +1
Query: 607 LKDQGNQMYKNSEFHSAITYYNIALQQGGNIHARFYANRAAAYISRNWAGDIYAALLDSI 786
LK++GNQ+ K+ + AI+ YN L+ A Y NRA Y+ G A LD
Sbjct: 608 LKEEGNQLVKDKNYKDAISKYNECLKINSKACA-IYTNRALCYLK---LGQFEEAKLDCD 663
Query: 787 KALEIDYSHIKSHFRI 834
KAL+ID ++K+ +R+
Sbjct: 664 KALQIDSKNVKASYRL 679
Score = 36.2 bits (82), Expect = 0.12
Identities = 33/111 (29%), Positives = 53/111 (47%), Gaps = 7/111 (6%)
Frame = +1
Query: 526 KSSINYTTNLSDVDKESGVEMLPH------LNILKDQGNQMYKNSEFHSAITYYNIALQQ 687
K+ IN +LS ++ + L N K +GN+ + + ++ A+ YY +L
Sbjct: 183 KTVINNKAHLSKIETKIDTAGLTEKEKNFLANREKGKGNEAFYSGDYEEAVMYYTRSLSA 242
Query: 688 GGNIHARFYANRAAAYIS-RNWAGDIYAALLDSIKALEIDYSHIKSHFRIA 837
A Y NRA A I + W+ +AL D KALE++ +IK+ R A
Sbjct: 243 LPT--ATAYNNRAQAEIKLQRWS----SALEDCEKALELEPGNIKALLRRA 287
>sp|Q9UNE7|STUB1_HUMAN STIP1 homology and U box-containing protein 1 (STIP1 homology and
U-box containing protein 1) (Carboxy terminus of
Hsp70-interacting protein) (E3 ubiquitin protein ligase
CHIP) (CLL-associated antigen KW-8) (Antigen NY-CO-7)
Length = 303
Score = 48.1 bits (113), Expect = 3e-05
Identities = 28/91 (30%), Positives = 46/91 (50%)
Frame = +1
Query: 583 EMLPHLNILKDQGNQMYKNSEFHSAITYYNIALQQGGNIHARFYANRAAAYISRNWAGDI 762
E P LK+QGN+++ ++ A Y A+ + + A +Y NRA Y+
Sbjct: 21 EKSPSAQELKEQGNRLFVGRKYPEAAACYGRAITRNPLV-AVYYTNRALCYLKMQQHEQ- 78
Query: 763 YAALLDSIKALEIDYSHIKSHFRIAQCLFQL 855
AL D +ALE+D +K+HF + QC ++
Sbjct: 79 --ALADCRRALELDGQSVKAHFFLGQCQLEM 107
>sp|Q9WUD1|STUB1_MOUSE STIP1 homology and U box-containing protein 1 (STIP1 homology and
U-box containing protein 1) (Carboxy terminus of
Hsp70-interacting protein) (E3 ubiquitin protein ligase
CHIP)
Length = 304
Score = 47.8 bits (112), Expect = 4e-05
Identities = 27/88 (30%), Positives = 45/88 (51%)
Frame = +1
Query: 592 PHLNILKDQGNQMYKNSEFHSAITYYNIALQQGGNIHARFYANRAAAYISRNWAGDIYAA 771
P LK+QGN+++ ++ A Y A+ + + A +Y NRA Y+ A
Sbjct: 25 PSAQELKEQGNRLFVGRKYPEAAACYGRAITRNPLV-AVYYTNRALCYLKMQQPEQ---A 80
Query: 772 LLDSIKALEIDYSHIKSHFRIAQCLFQL 855
L D +ALE+D +K+HF + QC ++
Sbjct: 81 LADCRRALELDGQSVKAHFFLGQCQLEM 108
>sp|P38825|YHR7_YEAST Hypothetical 71.9 kDa protein in ERP5-ORC6 intergenic region
Length = 639
Score = 45.4 bits (106), Expect = 2e-04
Identities = 29/77 (37%), Positives = 41/77 (53%)
Frame = +1
Query: 607 LKDQGNQMYKNSEFHSAITYYNIALQQGGNIHARFYANRAAAYISRNWAGDIYAALLDSI 786
LK++GN + F+ AI YY A++ N FY+N +A YIS GD+ + +
Sbjct: 130 LKNRGNHFFTAKNFNEAIKYYQYAIELDPN-EPVFYSNISACYIS---TGDLEKVIEFTT 185
Query: 787 KALEIDYSHIKSHFRIA 837
KALEI H K+ R A
Sbjct: 186 KALEIKPDHSKALLRRA 202
>sp|Q5ZHY5|STUB1_CHICK STIP1 homology and U box-containing protein 1 (STIP1 homology and
U-box containing protein 1)
Length = 314
Score = 45.4 bits (106), Expect = 2e-04
Identities = 25/82 (30%), Positives = 43/82 (52%)
Frame = +1
Query: 610 KDQGNQMYKNSEFHSAITYYNIALQQGGNIHARFYANRAAAYISRNWAGDIYAALLDSIK 789
K+QGN+++ ++ A Y A+ + + A +Y NRA Y+ AL D +
Sbjct: 40 KEQGNRLFGGRKYPEAAAAYGRAINRNPLV-AVYYTNRALCYLKMQQHDK---ALADCKR 95
Query: 790 ALEIDYSHIKSHFRIAQCLFQL 855
ALE+D +K+HF + QC ++
Sbjct: 96 ALELDGQSVKAHFFLGQCQMEM 117
>sp|Q9QYI3|DNJC7_MOUSE DnaJ homolog subfamily C member 7 (Tetratricopeptide repeat protein
2) (TPR repeat protein 2) (MDj11)
Length = 494
Score = 45.4 bits (106), Expect = 2e-04
Identities = 26/82 (31%), Positives = 42/82 (51%)
Frame = +1
Query: 610 KDQGNQMYKNSEFHSAITYYNIALQQGGNIHARFYANRAAAYISRNWAGDIYAALLDSIK 789
K+QGN Y +++ A YY A+ N +A +Y NRAA + G AL D+ +
Sbjct: 32 KEQGNAYYAKKDYNEAYNYYTKAIDMCPN-NASYYGNRAATLM---MLGRFREALGDAQQ 87
Query: 790 ALEIDYSHIKSHFRIAQCLFQL 855
++ +D S ++ H R +C L
Sbjct: 88 SVRLDDSFVRGHLREGKCHLSL 109
>sp|Q8R3H9|TTC4_MOUSE Tetratricopeptide repeat protein 4 (TPR repeat protein 4)
Length = 386
Score = 43.1 bits (100), Expect = 0.001
Identities = 25/85 (29%), Positives = 48/85 (56%), Gaps = 3/85 (3%)
Frame = +1
Query: 610 KDQGNQMYKNSEFHSAITYYNIALQQ---GGNIHARFYANRAAAYISRNWAGDIYAALLD 780
KD+GN +K ++ A+ Y+ L++ +++A Y NRAAA + + G++ ++L D
Sbjct: 83 KDEGNDYFKEKDYKKAVLSYSEGLKKKCADPDLNAVLYTNRAAA---QYYLGNVRSSLND 139
Query: 781 SIKALEIDYSHIKSHFRIAQCLFQL 855
+ A ++ H+K+ R A C +L
Sbjct: 140 VLAAKKLKPGHLKAIIRGALCHLEL 164
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.316 0.132 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 94,123,956
Number of Sequences: 369166
Number of extensions: 1966693
Number of successful extensions: 5439
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5181
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5416
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8438301375
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)