Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_003_O01
(855 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q01241|NF70_LOLPE 70 kDa neurofilament protein (NF70) 164 4e-40
sp|Q01240|NF60_LOLPE 60 kDa neurofilament protein (NF60) 164 4e-40
sp|P90901|IFA1_CAEEL Intermediate filament protein ifa-1 (I... 136 6e-32
sp|P31732|OV71_ONCVO Muscle cell intermediate filament prot... 134 2e-31
sp|Q17107|AV71_ACAVI Muscle cell intermediate filament prot... 132 1e-30
sp|O02365|IFA2_CAEEL Intermediate filament protein ifa-2 (I... 126 9e-29
sp|P90900|IFA4_CAEEL Intermediate filament protein ifa-4 (I... 117 3e-26
sp|P23731|IFEB_ASCSU Intermediate filament protein B (IF-B) 114 3e-25
sp|Q21065|IFA3_CAEEL Intermediate filament protein ifa-3 (I... 113 8e-25
sp|Q19289|IFB1_CAEEL Intermediate filament protein ifb-1 (I... 111 2e-24
>sp|Q01241|NF70_LOLPE 70 kDa neurofilament protein (NF70)
Length = 615
Score = 164 bits (414), Expect = 4e-40
Identities = 84/187 (44%), Positives = 119/187 (63%)
Frame = +3
Query: 3 QNLRETMDFEKQIHETEVKELTTVAYRDPTKENMMYWQSEMGRALRDIQTEYENKLSGIK 182
Q L E ++F+K +H E+KEL +AYRD T EN +W++E+ +A+RDIQ EY+ K ++
Sbjct: 263 QTLEEELEFQKDVHAQELKELAALAYRDTTAENREFWRNELAQAIRDIQQEYDAKCDQMR 322
Query: 183 QEMEASYGLQMQEIRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEYEHNL 362
++EA Y L++QE RT T+ N+E T KEEN+++KS + +R+R+ADLE +NA+ E
Sbjct: 323 GDIEAYYNLKVQEFRTGATKQNMEVTRNKEENTKLKSNMTEIRNRLADLEARNAQLERTN 382
Query: 363 NMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTELQSLLDAKLGLELEIAAYAKLL 542
+ + EE R EL+ + L ELQ L+D KL LELEIAAY KLL
Sbjct: 383 QDLLRDLEEKDRQNELESCQYKEEITKLRGEMESILKELQDLMDIKLSLELEIAAYRKLL 442
Query: 543 EFEESRV 563
E EESRV
Sbjct: 443 EGEESRV 449
>sp|Q01240|NF60_LOLPE 60 kDa neurofilament protein (NF60)
Length = 511
Score = 164 bits (414), Expect = 4e-40
Identities = 84/187 (44%), Positives = 119/187 (63%)
Frame = +3
Query: 3 QNLRETMDFEKQIHETEVKELTTVAYRDPTKENMMYWQSEMGRALRDIQTEYENKLSGIK 182
Q L E ++F+K +H E+KEL +AYRD T EN +W++E+ +A+RDIQ EY+ K ++
Sbjct: 263 QTLEEELEFQKDVHAQELKELAALAYRDTTAENREFWRNELAQAIRDIQQEYDAKCDQMR 322
Query: 183 QEMEASYGLQMQEIRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEYEHNL 362
++EA Y L++QE RT T+ N+E T KEEN+++KS + +R+R+ADLE +NA+ E
Sbjct: 323 GDIEAYYNLKVQEFRTGATKQNMEVTRNKEENTKLKSNMTEIRNRLADLEARNAQLERTN 382
Query: 363 NMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTELQSLLDAKLGLELEIAAYAKLL 542
+ + EE R EL+ + L ELQ L+D KL LELEIAAY KLL
Sbjct: 383 QDLLRDLEEKDRQNELESCQYKEEITKLRGEMESILKELQDLMDIKLSLELEIAAYRKLL 442
Query: 543 EFEESRV 563
E EESRV
Sbjct: 443 EGEESRV 449
>sp|P90901|IFA1_CAEEL Intermediate filament protein ifa-1 (Intermediate filament protein
A1) (IF-A1) (Cel IF A1)
Length = 575
Score = 136 bits (343), Expect = 6e-32
Identities = 72/186 (38%), Positives = 112/186 (60%)
Frame = +3
Query: 3 QNLRETMDFEKQIHETEVKELTTVAYRDPTKENMMYWQSEMGRALRDIQTEYENKLSGIK 182
Q L E +DF +++H+ E+KEL T+A RD T EN ++++E+ A+RDI+ EY+ + +
Sbjct: 236 QTLLEEIDFLRRVHDNEIKELQTLASRDTTPENREFFKNELSSAIRDIREEYDQVNNVHR 295
Query: 183 QEMEASYGLQMQEIRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEYEHNL 362
+ME+ Y L++QEI+T R N+E + KEE R+++Q+ +R ++ADLE +N+ E +
Sbjct: 296 NDMESWYRLKVQEIQTQSARQNMEQGYAKEEVKRLRTQLSDLRGKLADLESRNSLLEKQI 355
Query: 363 NMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTELQSLLDAKLGLELEIAAYAKLL 542
+ E+ QRS E L A + ELQ LLD K L+ EIA Y K+L
Sbjct: 356 QELNYQLEDDQRSYEAALNDRDSQIRKMREECQALMVELQMLLDTKQTLDAEIAIYRKML 415
Query: 543 EFEESR 560
E EE+R
Sbjct: 416 EGEENR 421
Score = 34.7 bits (78), Expect = 0.35
Identities = 16/39 (41%), Positives = 22/39 (56%)
Frame = +1
Query: 736 TERETTDYQSKRMKFRRTTKGNISIAETDLTGSFIILEN 852
T R + R F+R+ KGN+SI E G FI+L+N
Sbjct: 445 TMRVVKGETASRQSFQRSAKGNVSIHEASPDGKFIVLQN 483
>sp|P31732|OV71_ONCVO Muscle cell intermediate filament protein OV71
Length = 432
Score = 134 bits (338), Expect = 2e-31
Identities = 72/186 (38%), Positives = 110/186 (59%)
Frame = +3
Query: 3 QNLRETMDFEKQIHETEVKELTTVAYRDPTKENMMYWQSEMGRALRDIQTEYENKLSGIK 182
Q L E +DF +++H+ E+ EL +A RD T EN Y+++E+ A+RDI+ EY+ + +
Sbjct: 91 QTLLEEIDFMRRVHDQEISELQAMAARDTTSENREYFKNELSSAIRDIRAEYDQICNMNR 150
Query: 183 QEMEASYGLQMQEIRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEYEHNL 362
+ME+ Y L++QEI+T TR N+E + KEE R++ Q+ +R ++ADLE +N+ E +
Sbjct: 151 TDMESWYKLKVQEIQTQSTRQNLEQGYAKEEVKRLRVQLSDLRGKLADLEGRNSLLEKQM 210
Query: 363 NMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTELQSLLDAKLGLELEIAAYAKLL 542
+ E+ QRS E L A + ELQ LLD K L+ EIA Y K+L
Sbjct: 211 QELNYQLEDDQRSYEAALNDRDAQIRKMREECQALMMELQMLLDTKQTLDAEIAIYRKML 270
Query: 543 EFEESR 560
E EE+R
Sbjct: 271 EGEENR 276
Score = 34.3 bits (77), Expect = 0.45
Identities = 14/30 (46%), Positives = 20/30 (66%)
Frame = +1
Query: 763 SKRMKFRRTTKGNISIAETDLTGSFIILEN 852
+ R F+R+ KGN+SI + G FI+LEN
Sbjct: 309 ASRTSFQRSAKGNVSIQDASSDGKFILLEN 338
>sp|Q17107|AV71_ACAVI Muscle cell intermediate filament protein AV71
Length = 394
Score = 132 bits (332), Expect = 1e-30
Identities = 72/186 (38%), Positives = 109/186 (58%)
Frame = +3
Query: 3 QNLRETMDFEKQIHETEVKELTTVAYRDPTKENMMYWQSEMGRALRDIQTEYENKLSGIK 182
Q L E +DF +++H+ E+ EL +A RD T EN Y+++E+ A+RDI+ EY+ + +
Sbjct: 53 QTLLEEIDFMRRVHDQEITELQAMAARDTTPENREYFKNELSSAIRDIRAEYDQICNINR 112
Query: 183 QEMEASYGLQMQEIRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEYEHNL 362
+ME+ Y L++QEI+T TR N+E + KEE R++ Q+ +R ++ADLE +N+ E
Sbjct: 113 TDMESWYKLKVQEIQTQSTRQNLEQGYAKEEVKRLRVQLSELRGKLADLEGRNSLLEKQT 172
Query: 363 NMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTELQSLLDAKLGLELEIAAYAKLL 542
+ E+ QRS E L A + ELQ LLD K L+ EIA Y K+L
Sbjct: 173 QELNYQLEDDQRSYEAALNDRDAQIRKMREECQALMMELQMLLDTKQTLDAEIAIYRKML 232
Query: 543 EFEESR 560
E EE+R
Sbjct: 233 EGEENR 238
Score = 30.0 bits (66), Expect = 8.5
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +1
Query: 763 SKRMKFRRTTKGNISIAETDLTGSFIILEN 852
+ R F+R+ KGN+SI + G +I+ EN
Sbjct: 271 ASRTSFQRSAKGNVSIQDAASDGKYILSEN 300
>sp|O02365|IFA2_CAEEL Intermediate filament protein ifa-2 (Intermediate filament protein
A2) (IF-A2) (Cel IF A2)
Length = 581
Score = 126 bits (316), Expect = 9e-29
Identities = 70/194 (36%), Positives = 107/194 (55%)
Frame = +3
Query: 3 QNLRETMDFEKQIHETEVKELTTVAYRDPTKENMMYWQSEMGRALRDIQTEYENKLSGIK 182
+ + E +DF K+ ETE+KEL A RD T EN Y+++E+ A+RDI+ EY+ ++G +
Sbjct: 238 KTILEEIDFMKRGFETELKELQAQAARDTTSENREYFKNELANAMRDIRAEYDQIMNGNR 297
Query: 183 QEMEASYGLQMQEIRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEYEHNL 362
+ME+ Y L++QEI T R N E + KEE R+++Q +R +++DLE +N E +
Sbjct: 298 NDMESWYQLRVQEINTQSNRQNAENNYQKEEVKRLRNQTSELRQKLSDLESRNLLLEKQI 357
Query: 363 NMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTELQSLLDAKLGLELEIAAYAKLL 542
+ E+ QRS E L A + ELQ LLD K L+ E+ Y ++L
Sbjct: 358 EDLNYQLEDDQRSYEAALNDKDAQIRKLREECQALMVELQMLLDTKQTLDGELKVYRQML 417
Query: 543 EFEESRVNSNTEGN 584
E N+EGN
Sbjct: 418 E-------GNSEGN 424
Score = 37.0 bits (84), Expect = 0.070
Identities = 17/39 (43%), Positives = 23/39 (58%)
Frame = +1
Query: 736 TERETTDYQSKRMKFRRTTKGNISIAETDLTGSFIILEN 852
T R S R ++R+ KGN++I ET G F+ILEN
Sbjct: 447 TMRVVKGEHSSRTSYQRSAKGNVAIKETSPEGKFVILEN 485
>sp|P90900|IFA4_CAEEL Intermediate filament protein ifa-4 (Intermediate filament protein
A4) (IF-A4) (Cel IF A4)
Length = 575
Score = 117 bits (294), Expect = 3e-26
Identities = 63/186 (33%), Positives = 107/186 (57%)
Frame = +3
Query: 3 QNLRETMDFEKQIHETEVKELTTVAYRDPTKENMMYWQSEMGRALRDIQTEYENKLSGIK 182
Q L E DF ++IH++E+ +L +A RD T EN Y+++E+ A+R+I++EY++ + +
Sbjct: 237 QTLLEECDFIRRIHDSEIHDLLAMASRDTTVENREYFKNELSSAIREIRSEYDHVSNTTR 296
Query: 183 QEMEASYGLQMQEIRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEYEHNL 362
++E+ Y L++QEI T +R+ +E + +EE R+++ + MR ++ADLE +N E +
Sbjct: 297 TDIESWYKLKVQEIHTQNSRNCLEQNYAREEVKRLRTTLGDMRGKMADLEGRNLLLEKQI 356
Query: 363 NMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTELQSLLDAKLGLELEIAAYAKLL 542
+ EE RS E L + ELQ L+D K L+ EI Y K+L
Sbjct: 357 EDLNYQMEEDMRSYEQSLNDKDTSINKLRDESKILMVELQMLIDTKQTLDAEIVIYRKML 416
Query: 543 EFEESR 560
+ EE+R
Sbjct: 417 DGEENR 422
Score = 36.2 bits (82), Expect = 0.12
Identities = 25/80 (31%), Positives = 40/80 (50%)
Frame = +3
Query: 171 SGIKQEMEASYGLQMQEIRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEY 350
S + M G IR S+TR+ E + E N R+ S I+ +R LE QN +
Sbjct: 46 SNLTSGMSPFGGHAASAIRESRTREKKEMS---ELNDRLASYIEKVRF----LEAQNRKM 98
Query: 351 EHNLNMIRGNYEETQRSLEL 410
E +LN++RG + S+++
Sbjct: 99 EKDLNLLRGKWGHDSTSVKV 118
Score = 35.0 bits (79), Expect = 0.27
Identities = 17/37 (45%), Positives = 25/37 (67%)
Frame = +1
Query: 745 ETTDYQSKRMKFRRTTKGNISIAETDLTGSFIILENI 855
ETT + S + R+ KGNI+I E + +G +I+LENI
Sbjct: 453 ETTSHSS----YSRSAKGNIAIQEAEPSGKYIVLENI 485
>sp|P23731|IFEB_ASCSU Intermediate filament protein B (IF-B)
Length = 589
Score = 114 bits (286), Expect = 3e-25
Identities = 65/187 (34%), Positives = 105/187 (56%)
Frame = +3
Query: 3 QNLRETMDFEKQIHETEVKELTTVAYRDPTKENMMYWQSEMGRALRDIQTEYENKLSGIK 182
Q L E ++F +++HE EVKEL + + P + ++++E+ A+RDI+ EY+ K
Sbjct: 248 QTLMEELEFLRRVHEQEVKELQALLAQAPA-DTREFFKNELALAIRDIKDEYDYIAKQGK 306
Query: 183 QEMEASYGLQMQEIRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEYEHNL 362
Q+ME+ Y L++ E++ S R N+E+++ +EE R++ I +R ++ DLE +NA E +
Sbjct: 307 QDMESWYKLKVSEVQGSANRANMESSYQREEVKRMRDNIGDLRGKLGDLEAKNALLEKEV 366
Query: 363 NMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTELQSLLDAKLGLELEIAAYAKLL 542
+ + QR E L + ELQ+LLD K L+ EIA Y K+L
Sbjct: 367 QNLNYQLNDDQRQYEAALNDRDATLRRMREECQTLVAELQALLDTKQMLDAEIAIYRKML 426
Query: 543 EFEESRV 563
E EESRV
Sbjct: 427 EGEESRV 433
Score = 35.0 bits (79), Expect = 0.27
Identities = 15/30 (50%), Positives = 21/30 (70%)
Frame = +1
Query: 763 SKRMKFRRTTKGNISIAETDLTGSFIILEN 852
S + F+R+ KGN++I+E D G FI LEN
Sbjct: 465 STKTTFQRSAKGNVTISECDPNGKFITLEN 494
>sp|Q21065|IFA3_CAEEL Intermediate filament protein ifa-3 (Intermediate filament protein
A3) (IF-A3) (Cel IF A3)
Length = 581
Score = 113 bits (282), Expect = 8e-25
Identities = 62/177 (35%), Positives = 98/177 (55%)
Frame = +3
Query: 15 ETMDFEKQIHETEVKELTTVAYRDPTKENMMYWQSEMGRALRDIQTEYENKLSGIKQEME 194
E +DF K+ ETE+K+L A RD T EN Y+++E+ ++RDI+ EY+ ++G + ++E
Sbjct: 242 EEIDFMKRGFETELKDLQAQAARDTTSENREYFKNELMNSIRDIRAEYDRFMAGNRNDLE 301
Query: 195 ASYGLQMQEIRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEYEHNLNMIR 374
+ +++QEI T R N E H ++E R+ SQ+ ++S+ A+L +N E L +
Sbjct: 302 SWSQIRVQEINTQTNRQNAEINHKRDEVKRLHSQVSELKSKHAELAARNGLLEKQLEDLN 361
Query: 375 GNYEETQRSLELKLMSXXXXXXXXXXXXSAALTELQSLLDAKLGLELEIAAYAKLLE 545
E+ QRS E L A L ELQ LLD K L+ E+ Y ++LE
Sbjct: 362 YQLEDDQRSYEAALNDKDAQVRKLREECQALLVELQMLLDTKQTLDGELKVYRRMLE 418
Score = 36.2 bits (82), Expect = 0.12
Identities = 17/39 (43%), Positives = 22/39 (56%)
Frame = +1
Query: 736 TERETTDYQSKRMKFRRTTKGNISIAETDLTGSFIILEN 852
T R S R ++R+ KGN+SI E G F+ILEN
Sbjct: 447 TMRVVKGEHSSRTSYQRSAKGNVSIKEVSPEGKFVILEN 485
>sp|Q19289|IFB1_CAEEL Intermediate filament protein ifb-1 (Intermediate filament protein
B1) (IF-B1) (Cel IF B1)
Length = 589
Score = 111 bits (278), Expect = 2e-24
Identities = 63/187 (33%), Positives = 105/187 (56%)
Frame = +3
Query: 3 QNLRETMDFEKQIHETEVKELTTVAYRDPTKENMMYWQSEMGRALRDIQTEYENKLSGIK 182
Q L E ++F +++HE EVKEL + + P + ++++E+ A+RDI+ EY+ K
Sbjct: 248 QTLMEELEFLRRVHEQEVKELQALLAQAPA-DTREFFKNELALAIRDIKDEYDIIAKQGK 306
Query: 183 QEMEASYGLQMQEIRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEYEHNL 362
Q+ME+ Y L++ E++ S R N+E+T+ ++E R++ I +R ++ DLE +N+ E +
Sbjct: 307 QDMESWYKLKVSEVQGSANRANMESTYQRDEVKRMRDNIGDLRGKLGDLENKNSLLEKEV 366
Query: 363 NMIRGNYEETQRSLELKLMSXXXXXXXXXXXXSAALTELQSLLDAKLGLELEIAAYAKLL 542
+ + QR E L + ELQ+LLD K L+ EIA Y K+L
Sbjct: 367 QNLNYQLTDDQRQYEAALNDRDATLRRMREECQTLVAELQALLDTKQMLDAEIAIYRKML 426
Query: 543 EFEESRV 563
E EE+RV
Sbjct: 427 EGEETRV 433
Score = 34.3 bits (77), Expect = 0.45
Identities = 15/30 (50%), Positives = 21/30 (70%)
Frame = +1
Query: 763 SKRMKFRRTTKGNISIAETDLTGSFIILEN 852
S + F+R+ KGN++I+E D G FI LEN
Sbjct: 465 STKTTFQRSAKGNVTISECDPNGKFIKLEN 494
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 67,394,167
Number of Sequences: 369166
Number of extensions: 1041401
Number of successful extensions: 3988
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3524
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3813
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8438301375
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)