Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_003_N09
(869 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q13283|G3BP_HUMAN Ras-GTPase-activating protein binding ... 104 4e-22
sp|O94260|G3BP_SCHPO Putative G3BP-like protein 102 1e-21
sp|Q9UN86|G3B2_HUMAN Ras-GTPase-activating protein binding ... 100 7e-21
sp|P97379|G3B2_MOUSE Ras-GTPase-activating protein binding ... 99 1e-20
sp|P97855|G3BP_MOUSE Ras-GTPase-activating protein binding ... 96 1e-19
sp|P53741|BRE5_YEAST UBP3-associated protein BRE5 50 8e-06
sp|Q10100|NTF2_SCHPO Nuclear transport factor 2 (NTF-2) 50 8e-06
sp|Q6BWC0|NTF2_DEBHA Nuclear transport factor 2 (NTF-2) 49 1e-05
sp|Q9C7F5|NTF2_ARATH Nuclear transport factor 2 (NTF-2) 48 3e-05
sp|Q9P926|NTF2_CANAL Nuclear transport factor 2 (NTF-2) 48 3e-05
>sp|Q13283|G3BP_HUMAN Ras-GTPase-activating protein binding protein 1 (GAP SH3-domain
binding protein 1) (G3BP-1) (DNA helicase VIII)
(HDH-VIII)
Length = 466
Score = 104 bits (259), Expect = 4e-22
Identities = 75/254 (29%), Positives = 127/254 (50%), Gaps = 28/254 (11%)
Frame = +3
Query: 30 MDRELITTVSQEFVLQFYTIMNKHPGSLHRFYKEESTLIRDEVHAQG--------QNEIH 185
M++ V +EFV Q+YT++N+ P LHRFY + S+ + + + G Q EIH
Sbjct: 3 MEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKEIH 62
Query: 186 KYYMNLELSNCKAVVLSLDAVKSHGKSILIQVTGEIANNGCDLRRFMQSFLLVEQD--LG 359
+ M+ +NC + +DA + +++QV G ++NN LRRFMQ+F+L +
Sbjct: 63 RKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSVAN 122
Query: 360 NFYVLNDIFRYQDQTF-----KVEDVEEAPVVEHESKNE------DIHGEINSWNELSRN 506
FYV NDIFRYQD+ F + ++ E V E E + + D G +S +
Sbjct: 123 KFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQAVVSND 182
Query: 507 CELNNEQIPQSPQLIEHEEGSEEFINYKIMNEEKDISSEIPVEIDSQQ-------DIGQK 665
E + E+ P+ E +E ++ +I E+ + E D+Q+ DI Q
Sbjct: 183 MEEHLEEPVAEPEPDPEPEPEQEPVS-EIQEEKPEPVLEETAPEDAQKSSSPAPADIAQT 241
Query: 666 MEEMNIKEKSWAAI 707
++E +++ SWA++
Sbjct: 242 VQE-DLRTFSWASV 254
>sp|O94260|G3BP_SCHPO Putative G3BP-like protein
Length = 434
Score = 102 bits (254), Expect = 1e-21
Identities = 60/137 (43%), Positives = 87/137 (63%), Gaps = 6/137 (4%)
Frame = +3
Query: 66 FVLQFYTIMNKHPGSLHRFYKEESTLIR-DEVHA----QGQNEIHKYYMNLELSNCKAVV 230
FV ++YT +NK P LH FY ++STLI DE + GQ EIH ++L+ NCK ++
Sbjct: 22 FVQEYYTYLNKEPNRLHCFYTKKSTLIHGDEGESISLCHGQQEIHNKILDLDFQNCKVLI 81
Query: 231 LSLDAVKSHGKSILIQVTGEIANNGCDLRRFMQSFLLVEQDLGNFYVLNDIFRYQDQTFK 410
++D++ S I+IQV GE++N G R+F Q+F L EQ G ++VLNDIFR+ +
Sbjct: 82 SNVDSLASSNGGIVIQVLGEMSNKGKLSRKFAQTFFLAEQPNG-YFVLNDIFRFLRE--D 138
Query: 411 VEDVEEAP-VVEHESKN 458
VE+ EE+P VE E K+
Sbjct: 139 VEEEEESPDAVEKEKKD 155
>sp|Q9UN86|G3B2_HUMAN Ras-GTPase-activating protein binding protein 2 (GAP SH3-domain
binding protein 2) (G3BP-2)
Length = 482
Score = 100 bits (248), Expect = 7e-21
Identities = 70/237 (29%), Positives = 120/237 (50%), Gaps = 17/237 (7%)
Frame = +3
Query: 30 MDRELITTVSQEFVLQFYTIMNKHPGSLHRFYKEESTLIRDEVHAQG--------QNEIH 185
M++ V +EFV Q+YT++NK P LHRFY S+ + V A G QN+IH
Sbjct: 3 MEKPSPLLVGREFVRQYYTLLNKAPEYLHRFYGRNSSYVHGGVDASGKPQEAVYGQNDIH 62
Query: 186 KYYMNLELSNCKAVVLSLDAVKSHGKSILIQVTGEIANNGCDLRRFMQSFLLVEQDL--G 359
++L S C + +DA + +++QV G ++N+G R+FMQ+F+L +
Sbjct: 63 HKVLSLNFSECHTKIRHVDAHATLSDGVVVQVMGLLSNSGQPERKFMQTFVLAPEGSVPN 122
Query: 360 NFYVLNDIFRYQDQTFKVEDVEEAPVVEHESKNEDIHGEINSWNELSRNCELNNEQIPQS 539
FYV ND+FRY+D+ F + P ++ ES++E E E+ S
Sbjct: 123 KFYVHNDMFRYEDEVFG----DSEPELDEESEDE---------------VEEEQEERQPS 163
Query: 540 PQLIEHEEGSEEFINYKIMN--EE--KDISSEIPVEIDSQ---QDIGQKMEEMNIKE 689
P+ ++ S + + + N EE ++ S E E +S+ +++ ++EE N++E
Sbjct: 164 PEPVQENANSGYYEAHPVTNGIEEPLEESSHEPEPEPESETKTEELKPQVEEKNLEE 220
>sp|P97379|G3B2_MOUSE Ras-GTPase-activating protein binding protein 2 (GAP SH3-domain
binding protein 2) (G3BP-2)
Length = 482
Score = 99.4 bits (246), Expect = 1e-20
Identities = 67/233 (28%), Positives = 113/233 (48%), Gaps = 12/233 (5%)
Frame = +3
Query: 30 MDRELITTVSQEFVLQFYTIMNKHPGSLHRFYKEESTLIRDEVHAQG--------QNEIH 185
M++ V +EFV Q+YT++NK P LHRFY S+ + V A G QN+IH
Sbjct: 3 MEKPSPLLVGREFVRQYYTLLNKAPEYLHRFYGRNSSYVHGGVDASGKPQEAVYGQNDIH 62
Query: 186 KYYMNLELSNCKAVVLSLDAVKSHGKSILIQVTGEIANNGCDLRRFMQSFLLVEQDL--G 359
++L S C + +DA + +++QV G ++N+G R+FMQ+F+L +
Sbjct: 63 HKVLSLNFSECHTKIRHVDAHATLSDGVVVQVMGLLSNSGQPERKFMQTFVLAPEGSVPN 122
Query: 360 NFYVLNDIFRYQDQTFKVEDVEEAPVVEHESKNEDIHGEINSWNELSRNCELNNEQIPQS 539
FYV ND+FRY+D+ F + P ++ ES++E E E S
Sbjct: 123 KFYVHNDMFRYEDEVFG----DSEPELDEESEDE---------------VEEEQEDRQPS 163
Query: 540 PQLIEHEEGSEEFINYKIMNEEKDISSEIPVEIDSQQDIGQKMEEM--NIKEK 692
P+ ++ S + + + N ++ E E + + + K EE+ ++EK
Sbjct: 164 PEPVQENANSAYYDAHPVTNGIEEPLEESSHEPEPEPESETKTEELKPQVEEK 216
>sp|P97855|G3BP_MOUSE Ras-GTPase-activating protein binding protein 1 (GAP SH3-domain
binding protein 1) (G3BP-1) (DNA helicase VIII)
(HDH-VIII)
Length = 465
Score = 95.9 bits (237), Expect = 1e-19
Identities = 55/159 (34%), Positives = 86/159 (54%), Gaps = 15/159 (9%)
Frame = +3
Query: 30 MDRELITTVSQEFVLQFYTIMNKHPGSLHRFYKEESTLIRDEVHAQG--------QNEIH 185
M++ V +EFV Q+YT++N+ P LHRFY + S+ + + G Q EIH
Sbjct: 3 MEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYAHGGLDSNGKPADAVYGQKEIH 62
Query: 186 KYYMNLELSNCKAVVLSLDAVKSHGKSILIQVTGEIANNGCDLRRFMQSFLLVEQD--LG 359
+ M+ +NC + +DA + +++QV G ++NN LRRFMQ+F+L +
Sbjct: 63 RKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSVAN 122
Query: 360 NFYVLNDIFRYQDQTF-----KVEDVEEAPVVEHESKNE 461
FYV NDIFRYQD+ F + ++ E V E E + +
Sbjct: 123 KFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEERQQ 161
>sp|P53741|BRE5_YEAST UBP3-associated protein BRE5
Length = 515
Score = 50.1 bits (118), Expect = 8e-06
Identities = 40/152 (26%), Positives = 71/152 (46%), Gaps = 21/152 (13%)
Frame = +3
Query: 66 FVLQFYTIMNKHPGSL-----------HRFYKEESTLIRDEV----HAQGQNEIHKYYMN 200
F+ +Y M P L H Y+ +ST +D+V G+ I+K++
Sbjct: 12 FLQNYYERMRTDPSKLAYFYASTAELTHTNYQSKSTNEKDDVLPTVKVTGRENINKFFSR 71
Query: 201 LELSNCKAVVLSLDAVKSH-----GKSILIQVTGEIANNGCDLRRFMQSFLLVEQDLGN- 362
+ + +++ L LD + KSILI TGE+ G + +F Q+F+L+ G+
Sbjct: 72 ND-AKVRSLKLKLDTIDFQYTGHLHKSILIMATGEMFWTGTPVYKFCQTFILLPSSNGST 130
Query: 363 FYVLNDIFRYQDQTFKVEDVEEAPVVEHESKN 458
F + NDI R+ +FK + +A + + +N
Sbjct: 131 FDITNDIIRFISNSFKPYVLTDASLSQSNEEN 162
>sp|Q10100|NTF2_SCHPO Nuclear transport factor 2 (NTF-2)
Length = 123
Score = 50.1 bits (118), Expect = 8e-06
Identities = 34/114 (29%), Positives = 59/114 (51%), Gaps = 2/114 (1%)
Frame = +3
Query: 54 VSQEFVLQFYTIMNKHPGSLHRFYKEESTLIRDEVHAQGQNEIHKYYMNLELSNCKAVVL 233
++ +F +Y + L Y+EES L + QG I + ++L + +
Sbjct: 7 LATQFTQFYYQTFDSDRSQLSSLYREESMLSFEGAQLQGTKAIVEKLVSLPFQRVQHRIS 66
Query: 234 SLDAVKSHGK-SILIQVTGEIANNGCDL-RRFMQSFLLVEQDLGNFYVLNDIFR 389
+LDA + S+++ VTGE+ + + +R+ Q F LV + GN+YVLND+FR
Sbjct: 67 TLDAQPTGTTGSVIVMVTGELLLDEEQMAQRYSQVFHLVNNN-GNYYVLNDLFR 119
>sp|Q6BWC0|NTF2_DEBHA Nuclear transport factor 2 (NTF-2)
Length = 124
Score = 49.3 bits (116), Expect = 1e-05
Identities = 37/115 (32%), Positives = 58/115 (50%), Gaps = 2/115 (1%)
Frame = +3
Query: 51 TVSQEFVLQFYTIMNKHPGSLHRFYKEESTLIRDEVHAQGQNEIHKYYMNLELSNCKAVV 230
TV+ EF +Y + L Y+E+S L + QG +I + ++L +
Sbjct: 7 TVASEFCNFYYQQFDSDRTQLGNLYREQSMLTFETSQLQGAKDIVEKLVSLPFQKVAHRI 66
Query: 231 LSLDAVK-SHGKSILIQVTGE-IANNGCDLRRFMQSFLLVEQDLGNFYVLNDIFR 389
+LDA S IL+ VTGE I ++ + +R+ Q F L+ D ++YV NDIFR
Sbjct: 67 STLDAQPGSPNGDILVMVTGELIIDDEQNAQRYSQVFHLI-PDGNSYYVFNDIFR 120
>sp|Q9C7F5|NTF2_ARATH Nuclear transport factor 2 (NTF-2)
Length = 126
Score = 48.1 bits (113), Expect = 3e-05
Identities = 36/114 (31%), Positives = 55/114 (48%), Gaps = 2/114 (1%)
Frame = +3
Query: 54 VSQEFVLQFYTIMNKHPGSLHRFYKEESTLIRDEVHAQGQNEIHKYYMNLELSNCKAVVL 233
VS+ FV +Y+ + + L Y+E S L + QG I +L CK +
Sbjct: 9 VSKAFVEHYYSTFDTNRVGLAGLYQEASMLTFEGQKIQGVQSIVAKLTSLPFQQCKHHIS 68
Query: 234 SLDAVKSHGKS-ILIQVTGEIANNGCD-LRRFMQSFLLVEQDLGNFYVLNDIFR 389
++D S S +L+ V+G + G + +F Q F L+ G+FYV NDIFR
Sbjct: 69 TVDCQPSGPASGMLVFVSGNLQLAGEEHALKFSQMFHLMPTPQGSFYVFNDIFR 122
>sp|Q9P926|NTF2_CANAL Nuclear transport factor 2 (NTF-2)
Length = 124
Score = 48.1 bits (113), Expect = 3e-05
Identities = 35/114 (30%), Positives = 55/114 (48%), Gaps = 2/114 (1%)
Frame = +3
Query: 54 VSQEFVLQFYTIMNKHPGSLHRFYKEESTLIRDEVHAQGQNEIHKYYMNLELSNCKAVVL 233
V+ EF +Y + L Y+ ES L + QG +I + +L +
Sbjct: 8 VATEFCNFYYNQFDSDRSQLGNLYRNESMLTFETSQLQGARDIVEKLASLPFQKVAHRIS 67
Query: 234 SLDAVKSHGKS-ILIQVTGEIA-NNGCDLRRFMQSFLLVEQDLGNFYVLNDIFR 389
+LDA + IL+ VTGE+ + + +R+ Q F L+ D G++YV NDIFR
Sbjct: 68 TLDAQPASANGDILVMVTGELLIDEEQNAQRYSQVFHLIP-DNGSYYVFNDIFR 120
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84,606,151
Number of Sequences: 369166
Number of extensions: 1497313
Number of successful extensions: 5350
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5317
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8598109270
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)