Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_003_N05
(711 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O95197|RTN3_HUMAN Reticulon-3 (Neuroendocrine-specific p... 80 7e-15
sp|Q9ES97|RTN3_MOUSE Reticulon-3 80 7e-15
sp|Q16799|RTN1_HUMAN Reticulon-1 (Neuroendocrine-specific p... 77 5e-14
sp|Q5IS59|RTN1_PANTR Reticulon-1 77 5e-14
sp|Q64548|RTN1_RAT Reticulon-1 (Neuroendocrine-specific pro... 75 1e-13
sp|Q8K0T0|RTN1_MOUSE Reticulon-1 (Neuroendocrine-specific p... 75 1e-13
sp|Q9NQC3|RTN4_HUMAN Reticulon-4 (Neurite outgrowth inhibit... 74 4e-13
sp|Q9JK11|RTN4_RAT Reticulon-4 (Neurite outgrowth inhibitor... 74 4e-13
sp|Q99P72|RTN4_MOUSE Reticulon-4 (Neurite outgrowth inhibit... 73 7e-13
sp|O75298|RTN2_HUMAN Reticulon-2 (Neuroendocrine-specific p... 65 1e-10
>sp|O95197|RTN3_HUMAN Reticulon-3 (Neuroendocrine-specific protein-like 2) (NSP-like
protein II) (NSPLII)
Length = 236
Score = 79.7 bits (195), Expect = 7e-15
Identities = 57/185 (30%), Positives = 90/185 (48%), Gaps = 4/185 (2%)
Frame = +1
Query: 25 SIKSVISWKCPVVTGSILVTWVIFAVMVKTMSFISVIAYIGLILIMSSMLFRFYSNFIN- 201
++ +I W+ TG + T +I + + S ISV++Y+ L L+ ++ FR Y + I
Sbjct: 47 AVHDLIFWRDVKKTGFVFGTTLIMLLSLAAFSVISVVSYLILALLSVTISFRIYKSVIQA 106
Query: 202 ---NEASNPFQECLDKDVNLYLSKISDFVHFQTSEESSQFKSIIKYLNSIFLLESYADVG 372
+E +PF+ LD D+ L ++++ + K II+ +FL+E D
Sbjct: 107 VQKSEEGHPFKAYLDVDITLSSEAFHNYMNAAMVHINRALKLIIR----LFLVEDLVDSL 162
Query: 373 KFLVGNVAMVYVGMYFNLIDLVTLAVLSLLTGPKVYNLYRRQIDDAVMKAREHVGKAVAI 552
K V M YVG FN I L+ LA L + + P VY Y+ QID V AR+ V
Sbjct: 163 KLAVFMWLMTYVGAVFNGITLLILAELLIFSVPIVYEKYKTQIDHYVGIARDQTKSIVEK 222
Query: 553 AKEKL 567
+ KL
Sbjct: 223 IQAKL 227
>sp|Q9ES97|RTN3_MOUSE Reticulon-3
Length = 237
Score = 79.7 bits (195), Expect = 7e-15
Identities = 57/185 (30%), Positives = 90/185 (48%), Gaps = 4/185 (2%)
Frame = +1
Query: 25 SIKSVISWKCPVVTGSILVTWVIFAVMVKTMSFISVIAYIGLILIMSSMLFRFYSNFIN- 201
++ +I W+ TG + T +I + + S ISV++Y+ L L+ ++ FR Y + I
Sbjct: 48 AVHDLIFWRDVKKTGFVFGTTLIMLLSLAAFSVISVVSYLILALLSVTISFRVYKSVIQA 107
Query: 202 ---NEASNPFQECLDKDVNLYLSKISDFVHFQTSEESSQFKSIIKYLNSIFLLESYADVG 372
+E +PF+ LD D+ L ++++ + K II+ +FL+E D
Sbjct: 108 VQKSEEGHPFKAYLDVDITLSSEAFHNYMNAAMVHVNKALKLIIR----LFLVEDLVDSL 163
Query: 373 KFLVGNVAMVYVGMYFNLIDLVTLAVLSLLTGPKVYNLYRRQIDDAVMKAREHVGKAVAI 552
K V M YVG FN I L+ LA L + + P VY Y+ QID V AR+ V
Sbjct: 164 KLAVFMWLMTYVGAVFNGITLLILAELLVFSVPIVYEKYKTQIDHYVGIARDQTKSIVEK 223
Query: 553 AKEKL 567
+ KL
Sbjct: 224 IQAKL 228
>sp|Q16799|RTN1_HUMAN Reticulon-1 (Neuroendocrine-specific protein)
Length = 776
Score = 77.0 bits (188), Expect = 5e-14
Identities = 49/184 (26%), Positives = 93/184 (50%), Gaps = 7/184 (3%)
Frame = +1
Query: 37 VISWKCPVVTGSILVTWVIFAVMVKTMSFISVIAYIGLILIMSSMLFRFYSNFIN----N 204
++ W+ TG + ++++ + S +SV+AY+ L + +++ FR Y + +
Sbjct: 592 LLYWRDIKQTGIVFGSFLLLLFSLTQFSVVSVVAYLALAALSATISFRIYKSVLQAVQKT 651
Query: 205 EASNPFQECLDKDVNL---YLSKISDFVHFQTSEESSQFKSIIKYLNSIFLLESYADVGK 375
+ +PF+ L+ ++ L + K +D + F + S +K L +FL++ D K
Sbjct: 652 DEGHPFKAYLELEITLSQEQIQKYTDCLQFYVN-------STLKELRRLFLVQDLVDSLK 704
Query: 376 FLVGNVAMVYVGMYFNLIDLVTLAVLSLLTGPKVYNLYRRQIDDAVMKAREHVGKAVAIA 555
F V + YVG FN + L+ +AV+S+ T P VY ++ QID + R H+ VA
Sbjct: 705 FAVLMWLLTYVGALFNGLTLLLMAVVSMFTLPVVYVKHQAQIDQYLGLVRTHINAVVAKI 764
Query: 556 KEKL 567
+ K+
Sbjct: 765 QAKI 768
>sp|Q5IS59|RTN1_PANTR Reticulon-1
Length = 776
Score = 77.0 bits (188), Expect = 5e-14
Identities = 49/184 (26%), Positives = 93/184 (50%), Gaps = 7/184 (3%)
Frame = +1
Query: 37 VISWKCPVVTGSILVTWVIFAVMVKTMSFISVIAYIGLILIMSSMLFRFYSNFIN----N 204
++ W+ TG + ++++ + S +SV+AY+ L + +++ FR Y + +
Sbjct: 592 LLYWRDIKQTGIVFGSFLLLLFSLTQFSVVSVVAYLALAALSATISFRIYKSVLQAVQKT 651
Query: 205 EASNPFQECLDKDVNL---YLSKISDFVHFQTSEESSQFKSIIKYLNSIFLLESYADVGK 375
+ +PF+ L+ ++ L + K +D + F + S +K L +FL++ D K
Sbjct: 652 DEGHPFKAYLELEITLSQEQIQKYTDCLQFYVN-------STLKELRRLFLVQDLVDSLK 704
Query: 376 FLVGNVAMVYVGMYFNLIDLVTLAVLSLLTGPKVYNLYRRQIDDAVMKAREHVGKAVAIA 555
F V + YVG FN + L+ +AV+S+ T P VY ++ QID + R H+ VA
Sbjct: 705 FAVLMWLLTYVGALFNGLTLLLMAVVSMFTLPVVYVKHQAQIDQYLGLVRTHINAVVAKI 764
Query: 556 KEKL 567
+ K+
Sbjct: 765 QAKI 768
>sp|Q64548|RTN1_RAT Reticulon-1 (Neuroendocrine-specific protein) (S-rex)
Length = 777
Score = 75.5 bits (184), Expect = 1e-13
Identities = 47/181 (25%), Positives = 90/181 (49%), Gaps = 4/181 (2%)
Frame = +1
Query: 37 VISWKCPVVTGSILVTWVIFAVMVKTMSFISVIAYIGLILIMSSMLFRFYSNFIN----N 204
++ W+ TG + ++++ + S +SV+AY+ L + +++ FR Y + +
Sbjct: 593 LLYWRDIKQTGIVFGSFLLLLFSLTQFSVVSVVAYLALAALSATISFRIYKSVLQAVQKT 652
Query: 205 EASNPFQECLDKDVNLYLSKISDFVHFQTSEESSQFKSIIKYLNSIFLLESYADVGKFLV 384
+ +PF+ L+ ++ L +I + T S +K L +FL++ D KF V
Sbjct: 653 DEGHPFKAYLELEITLSQEQIQKY----TDCLQLYVNSTLKELRRLFLVQDLVDSLKFAV 708
Query: 385 GNVAMVYVGMYFNLIDLVTLAVLSLLTGPKVYNLYRRQIDDAVMKAREHVGKAVAIAKEK 564
+ YVG FN + L+ +AV+S+ T P VY ++ Q+D + R H+ VA + K
Sbjct: 709 LMWLLTYVGALFNGLTLLLMAVVSMFTLPVVYVKHQAQVDQYLGLVRTHINTVVAKIQAK 768
Query: 565 L 567
+
Sbjct: 769 I 769
>sp|Q8K0T0|RTN1_MOUSE Reticulon-1 (Neuroendocrine-specific protein)
Length = 780
Score = 75.5 bits (184), Expect = 1e-13
Identities = 47/181 (25%), Positives = 90/181 (49%), Gaps = 4/181 (2%)
Frame = +1
Query: 37 VISWKCPVVTGSILVTWVIFAVMVKTMSFISVIAYIGLILIMSSMLFRFYSNFIN----N 204
++ W+ TG + ++++ + S +SV+AY+ L + +++ FR Y + +
Sbjct: 596 LLYWRDIKQTGIVFGSFLLLLFSLTQFSVVSVVAYLALAALSATISFRIYKSVLQAVQKT 655
Query: 205 EASNPFQECLDKDVNLYLSKISDFVHFQTSEESSQFKSIIKYLNSIFLLESYADVGKFLV 384
+ +PF+ L+ ++ L +I + T S +K L +FL++ D KF V
Sbjct: 656 DEGHPFKAYLELEITLSQEQIQKY----TDCLQLYVNSTLKELRRLFLVQDLVDSLKFAV 711
Query: 385 GNVAMVYVGMYFNLIDLVTLAVLSLLTGPKVYNLYRRQIDDAVMKAREHVGKAVAIAKEK 564
+ YVG FN + L+ +AV+S+ T P VY ++ Q+D + R H+ VA + K
Sbjct: 712 LMWLLTYVGALFNGLTLLLMAVVSMFTLPVVYVKHQAQVDQYLGLVRTHINTVVAKIQAK 771
Query: 565 L 567
+
Sbjct: 772 I 772
>sp|Q9NQC3|RTN4_HUMAN Reticulon-4 (Neurite outgrowth inhibitor) (Nogo protein) (Foocen)
(Neuroendocrine-specific protein) (NSP)
(Neuroendocrine-specific protein C homolog) (RTN-x)
(Reticulon-5)
Length = 1192
Score = 73.9 bits (180), Expect = 4e-13
Identities = 51/188 (27%), Positives = 90/188 (47%), Gaps = 4/188 (2%)
Frame = +1
Query: 25 SIKSVISWKCPVVTGSILVTWVIFAVMVKTMSFISVIAYIGLILIMSSMLFRFYSNFIN- 201
S+ ++ W+ TG + + + + S +SV AYI L L+ ++ FR Y I
Sbjct: 1004 SVVDLLYWRDIKKTGVVFGASLFLLLSLTVFSIVSVTAYIALALLSVTISFRIYKGVIQA 1063
Query: 202 ---NEASNPFQECLDKDVNLYLSKISDFVHFQTSEESSQFKSIIKYLNSIFLLESYADVG 372
++ +PF+ L+ +V + + V ++ IK L +FL++ D
Sbjct: 1064 IQKSDEGHPFRAYLESEVAIS----EELVQKYSNSALGHVNCTIKELRRLFLVDDLVDSL 1119
Query: 373 KFLVGNVAMVYVGMYFNLIDLVTLAVLSLLTGPKVYNLYRRQIDDAVMKAREHVGKAVAI 552
KF V YVG FN + L+ LA++SL + P +Y ++ QID + A ++V A+A
Sbjct: 1120 KFAVLMWVFTYVGALFNGLTLLILALISLFSVPVIYERHQAQIDHYLGLANKNVKDAMAK 1179
Query: 553 AKEKLATL 576
+ K+ L
Sbjct: 1180 IQAKIPGL 1187
>sp|Q9JK11|RTN4_RAT Reticulon-4 (Neurite outgrowth inhibitor) (Nogo protein) (Foocen)
(Glut4 vesicle 20 kDa protein)
Length = 1163
Score = 73.9 bits (180), Expect = 4e-13
Identities = 52/188 (27%), Positives = 90/188 (47%), Gaps = 4/188 (2%)
Frame = +1
Query: 25 SIKSVISWKCPVVTGSILVTWVIFAVMVKTMSFISVIAYIGLILIMSSMLFRFYSNFIN- 201
S+ ++ W+ TG + + + + S +SV AYI L L+ ++ FR Y I
Sbjct: 975 SVVDLLYWRDIKKTGVVFGASLFLLLSLTVFSIVSVTAYIALALLSVTISFRIYKGVIQA 1034
Query: 202 ---NEASNPFQECLDKDVNLYLSKISDFVHFQTSEESSQFKSIIKYLNSIFLLESYADVG 372
++ +PF+ L+ +V + + V ++ S IK L +FL++ D
Sbjct: 1035 IQKSDEGHPFRAYLESEVAIS----EELVQKYSNSALGHVNSTIKELRRLFLVDDLVDSL 1090
Query: 373 KFLVGNVAMVYVGMYFNLIDLVTLAVLSLLTGPKVYNLYRRQIDDAVMKAREHVGKAVAI 552
KF V YVG FN + L+ LA++SL + P +Y ++ QID + A + V A+A
Sbjct: 1091 KFAVLMWVFTYVGALFNGLTLLILALISLFSIPVIYERHQVQIDHYLGLANKSVKDAMAK 1150
Query: 553 AKEKLATL 576
+ K+ L
Sbjct: 1151 IQAKIPGL 1158
>sp|Q99P72|RTN4_MOUSE Reticulon-4 (Neurite outgrowth inhibitor) (Nogo protein)
Length = 199
Score = 73.2 bits (178), Expect = 7e-13
Identities = 51/187 (27%), Positives = 89/187 (47%), Gaps = 4/187 (2%)
Frame = +1
Query: 28 IKSVISWKCPVVTGSILVTWVIFAVMVKTMSFISVIAYIGLILIMSSMLFRFYSNFIN-- 201
+ ++ W+ TG + + + + S +SV AYI L L+ ++ FR Y I
Sbjct: 12 VVDLLYWRDIKKTGVVFGASLFLLLSLTVFSIVSVTAYIALALLSVTISFRIYKGVIQAI 71
Query: 202 --NEASNPFQECLDKDVNLYLSKISDFVHFQTSEESSQFKSIIKYLNSIFLLESYADVGK 375
++ +PF+ L+ +V + + V ++ S IK L +FL++ D K
Sbjct: 72 QKSDEGHPFRAYLESEVAIS----EELVQKYSNSALGHVNSTIKELRRLFLVDDLVDSLK 127
Query: 376 FLVGNVAMVYVGMYFNLIDLVTLAVLSLLTGPKVYNLYRRQIDDAVMKAREHVGKAVAIA 555
F V YVG FN + L+ LA++SL + P +Y ++ QID + A + V A+A
Sbjct: 128 FAVLMWVFTYVGALFNGLTLLILALISLFSIPVIYERHQAQIDHYLGLANKSVKDAMAKI 187
Query: 556 KEKLATL 576
+ K+ L
Sbjct: 188 QAKIPGL 194
>sp|O75298|RTN2_HUMAN Reticulon-2 (Neuroendocrine-specific protein-like 1) (NSP-like
protein 1) (NSPLI)
Length = 545
Score = 65.5 bits (158), Expect = 1e-10
Identities = 43/166 (25%), Positives = 77/166 (46%), Gaps = 4/166 (2%)
Frame = +1
Query: 28 IKSVISWKCPVVTGSILVTWVIFAVMVKTMSFISVIAYIGLILIMSSMLFRFYSNFIN-- 201
+ ++ WK +G + ++ + + S +SV A++ L+L+ ++ R Y +
Sbjct: 345 VADLLYWKDTRTSGVVFTGLMVSLLCLLHFSIVSVAAHLALLLLCGTISLRVYRKVLQAV 404
Query: 202 --NEASNPFQECLDKDVNLYLSKISDFVHFQTSEESSQFKSIIKYLNSIFLLESYADVGK 375
+ +NPFQ LD D+ L + H + +S+ S L FL+E D K
Sbjct: 405 HRGDGANPFQAYLDVDLTLTREQTERLSH----QITSRVVSAATQLRHFFLVEDLVDSLK 460
Query: 376 FLVGNVAMVYVGMYFNLIDLVTLAVLSLLTGPKVYNLYRRQIDDAV 513
+ + +VG FN + L+ L V+ L T P +Y ++ QID V
Sbjct: 461 LALLFYILTFVGAIFNGLTLLILGVIGLFTIPLLYRQHQAQIDQYV 506
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 82,385,445
Number of Sequences: 369166
Number of extensions: 1699068
Number of successful extensions: 5425
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5413
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6267895215
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)