Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_003_M20
(820 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P38062|AMPM2_RAT Methionine aminopeptidase 2 (MetAP 2) (... 448 e-126
sp|O08663|AMPM2_MOUSE Methionine aminopeptidase 2 (MetAP 2)... 447 e-125
sp|P50579|AMPM2_HUMAN Methionine aminopeptidase 2 (MetAP 2)... 446 e-125
sp|P38174|AMPM2_YEAST Methionine aminopeptidase 2 (MetAP 2)... 332 7e-91
sp|Q6XMH7|AMPM2_ENCIN Methionine aminopeptidase 2 (MetAP 2)... 223 5e-58
sp|Q6XMH6|AMPM2_ENCHE Methionine aminopeptidase 2 (MetAP 2)... 213 4e-55
sp|Q8SR45|AMPM2_ENCCU Methionine aminopeptidase 2 (MetAP 2)... 212 9e-55
sp|P50581|YD43_CAEEL Hypothetical protein T27A8.3 in chromo... 161 2e-39
sp|P56218|AMPM_PYRFU Methionine aminopeptidase (MAP) (Pepti... 150 5e-36
sp|O58362|AMPM_PYRHO Methionine aminopeptidase (MAP) (Pepti... 144 2e-34
>sp|P38062|AMPM2_RAT Methionine aminopeptidase 2 (MetAP 2) (Peptidase M 2) (Initiation
factor 2-associated 67 kDa glycoprotein) (p67) (p67eIF2)
Length = 478
Score = 448 bits (1153), Expect = e-126
Identities = 214/273 (78%), Positives = 242/273 (88%), Gaps = 1/273 (0%)
Frame = +1
Query: 4 SRTLIHERGLDAGLAFPTGCSLNHVAAHYTPNAGDTTVLKYDDVCKIDFGVHVNGRIIDC 183
SR LI E GL+AGLAFPTGCSLN+ AAHYTPNAGDTTVL+YDD+CKIDFG H++GRIIDC
Sbjct: 204 SRKLIKENGLNAGLAFPTGCSLNNCAAHYTPNAGDTTVLQYDDICKIDFGTHISGRIIDC 263
Query: 184 AFTLTFNEKYDKLVEAVKDATNTGLREAGIDVRLCDIGEAIQETMESYEVELDGKTYKVK 363
AFT+TFN KYD L++AVKDATNTG++ AGIDVRLCD+GEAIQE MESYEVE+DGKTY+VK
Sbjct: 264 AFTVTFNPKYDILLKAVKDATNTGIKCAGIDVRLCDVGEAIQEVMESYEVEIDGKTYQVK 323
Query: 364 PIRNLNGHSIGPYQIHAGKTVPIVKGGEATKMEENEYYAIETFGSTGKGFVRDDLECSHY 543
PIRNLNGHSIGPY+IHAGKTVPIVKGGEAT+MEE E YAIETFGSTGKG V DD+ECSHY
Sbjct: 324 PIRNLNGHSIGPYRIHAGKTVPIVKGGEATRMEEGEVYAIETFGSTGKGVVHDDMECSHY 383
Query: 544 MKNFEIGHVPLRLARSKALLNTINKNFGTLAFCRRWLDRLGEERYIGALKNLTETGLVDP 723
MKNF++GHVP+RL R+K LLN IN+NFGTLAFCRRWLDRLGE +Y+ ALKNL + G+VDP
Sbjct: 384 MKNFDVGHVPIRLPRTKHLLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDP 443
Query: 724 YPPLCDIKGCYTAQW-NIQYY*TNM*EVVSRGD 819
YPPLCDIKG YTAQ+ + EVVSRGD
Sbjct: 444 YPPLCDIKGSYTAQFEHTILLRPTCKEVVSRGD 476
>sp|O08663|AMPM2_MOUSE Methionine aminopeptidase 2 (MetAP 2) (Peptidase M 2) (Initiation
factor 2-associated 67 kDa glycoprotein) (p67) (p67eIF2)
Length = 478
Score = 447 bits (1151), Expect = e-125
Identities = 214/273 (78%), Positives = 241/273 (88%), Gaps = 1/273 (0%)
Frame = +1
Query: 4 SRTLIHERGLDAGLAFPTGCSLNHVAAHYTPNAGDTTVLKYDDVCKIDFGVHVNGRIIDC 183
SR LI E GL+AGLAFPTGCSLN+ AAHYTPNAGDTTVL+YDD+CKIDFG H++GRIIDC
Sbjct: 204 SRKLIKENGLNAGLAFPTGCSLNNCAAHYTPNAGDTTVLQYDDICKIDFGTHISGRIIDC 263
Query: 184 AFTLTFNEKYDKLVEAVKDATNTGLREAGIDVRLCDIGEAIQETMESYEVELDGKTYKVK 363
AFT+TFN KYD L+ AVKDATNTG++ AGIDVRLCD+GEAIQE MESYEVE+DGKTY+VK
Sbjct: 264 AFTVTFNPKYDILLTAVKDATNTGIKCAGIDVRLCDVGEAIQEVMESYEVEIDGKTYQVK 323
Query: 364 PIRNLNGHSIGPYQIHAGKTVPIVKGGEATKMEENEYYAIETFGSTGKGFVRDDLECSHY 543
PIRNLNGHSIGPY+IHAGKTVPIVKGGEAT+MEE E YAIETFGSTGKG V DD+ECSHY
Sbjct: 324 PIRNLNGHSIGPYRIHAGKTVPIVKGGEATRMEEGEVYAIETFGSTGKGVVHDDMECSHY 383
Query: 544 MKNFEIGHVPLRLARSKALLNTINKNFGTLAFCRRWLDRLGEERYIGALKNLTETGLVDP 723
MKNF++GHVP+RL R+K LLN IN+NFGTLAFCRRWLDRLGE +Y+ ALKNL + G+VDP
Sbjct: 384 MKNFDVGHVPIRLPRTKHLLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDP 443
Query: 724 YPPLCDIKGCYTAQW-NIQYY*TNM*EVVSRGD 819
YPPLCDIKG YTAQ+ + EVVSRGD
Sbjct: 444 YPPLCDIKGSYTAQFEHTILLRPTCKEVVSRGD 476
>sp|P50579|AMPM2_HUMAN Methionine aminopeptidase 2 (MetAP 2) (Peptidase M 2) (Initiation
factor 2-associated 67 kDa glycoprotein) (p67) (p67eIF2)
Length = 478
Score = 446 bits (1147), Expect = e-125
Identities = 213/273 (78%), Positives = 241/273 (88%), Gaps = 1/273 (0%)
Frame = +1
Query: 4 SRTLIHERGLDAGLAFPTGCSLNHVAAHYTPNAGDTTVLKYDDVCKIDFGVHVNGRIIDC 183
SR LI E GL+AGLAFPTGCSLN+ AAHYTPNAGDTTVL+YDD+CKIDFG H++GRIIDC
Sbjct: 204 SRKLIKENGLNAGLAFPTGCSLNNCAAHYTPNAGDTTVLQYDDICKIDFGTHISGRIIDC 263
Query: 184 AFTLTFNEKYDKLVEAVKDATNTGLREAGIDVRLCDIGEAIQETMESYEVELDGKTYKVK 363
AFT+TFN KYD L++AVKDATNTG++ AGIDVRLCD+GEAIQE MESYEVE+DGKTY+VK
Sbjct: 264 AFTVTFNPKYDTLLKAVKDATNTGIKCAGIDVRLCDVGEAIQEVMESYEVEIDGKTYQVK 323
Query: 364 PIRNLNGHSIGPYQIHAGKTVPIVKGGEATKMEENEYYAIETFGSTGKGFVRDDLECSHY 543
PIRNLNGHSIG Y+IHAGKTVPIVKGGEAT+MEE E YAIETFGSTGKG V DD+ECSHY
Sbjct: 324 PIRNLNGHSIGQYRIHAGKTVPIVKGGEATRMEEGEVYAIETFGSTGKGVVHDDMECSHY 383
Query: 544 MKNFEIGHVPLRLARSKALLNTINKNFGTLAFCRRWLDRLGEERYIGALKNLTETGLVDP 723
MKNF++GHVP+RL R+K LLN IN+NFGTLAFCRRWLDRLGE +Y+ ALKNL + G+VDP
Sbjct: 384 MKNFDVGHVPIRLPRTKHLLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDP 443
Query: 724 YPPLCDIKGCYTAQW-NIQYY*TNM*EVVSRGD 819
YPPLCDIKG YTAQ+ + EVVSRGD
Sbjct: 444 YPPLCDIKGSYTAQFEHTILLRPTCKEVVSRGD 476
>sp|P38174|AMPM2_YEAST Methionine aminopeptidase 2 (MetAP 2) (Peptidase M 2)
Length = 421
Score = 332 bits (851), Expect = 7e-91
Identities = 167/261 (63%), Positives = 203/261 (77%), Gaps = 1/261 (0%)
Frame = +1
Query: 40 GLAFPTGCSLNHVAAHYTPNAGDTTVLKYDDVCKIDFGVHVNGRIIDCAFTLTFNEKYDK 219
G+ FPTG SLNH AAH+TPNAGD TVLKY+DV K+D+GV VNG IID AFT++F+ +YD
Sbjct: 159 GIGFPTGLSLNHCAAHFTPNAGDKTVLKYEDVMKVDYGVQVNGNIIDSAFTVSFDPQYDN 218
Query: 220 LVEAVKDATNTGLREAGIDVRLCDIGEAIQETMESYEVELDGKTYKVKPIRNLNGHSIGP 399
L+ AVKDAT TG++EAGIDVRL DIGEAIQE MESYEVE++G+TY+VKP RNL GHSI P
Sbjct: 219 LLAAVKDATYTGIKEAGIDVRLTDIGEAIQEVMESYEVEINGETYQVKPCRNLCGHSIAP 278
Query: 400 YQIHAGKTVPIVKGGEATKMEENEYYAIETFGSTGKGFVRDDLECSHYMKNFEIGHVPLR 579
Y+IH GK+VPIVK G+ TKMEE E++AIETFGSTG+G+V E SHY ++ E V
Sbjct: 279 YRIHGGKSVPIVKNGDTTKMEEGEHFAIETFGSTGRGYVTAGGEVSHYARSAEDHQVMPT 338
Query: 580 LARSKALLNTINKNFGTLAFCRRWLDRLGEERYIGALKNLTETGLVDPYPPLCDIKGCYT 759
L +K LL TI++NFGTL FCRR+LDRLG+E+Y+ AL NL GLV YPPL DI G YT
Sbjct: 339 LDSAKNLLKTIDRNFGTLPFCRRYLDRLGQEKYLFALNNLVRHGLVQDYPPLNDIPGSYT 398
Query: 760 AQW-NIQYY*TNM*EVVSRGD 819
AQ+ + + EVVS+GD
Sbjct: 399 AQFEHTILLHAHKKEVVSKGD 419
>sp|Q6XMH7|AMPM2_ENCIN Methionine aminopeptidase 2 (MetAP 2) (Peptidase M 2) (MetAP2)
(MAP-2)
Length = 358
Score = 223 bits (568), Expect = 5e-58
Identities = 122/279 (43%), Positives = 172/279 (61%), Gaps = 6/279 (2%)
Frame = +1
Query: 1 TSRTLIHERGLDAGLAFPTGCSLNHVAAHYTPNAGDTT-VLKYDDVCKIDFGVHVNGRII 177
++RTL+ E + G+ FP G S+N AAHYT N G+ VLK DDV K+DFG H NGRI+
Sbjct: 82 STRTLL-EGERNNGIGFPAGMSMNSCAAHYTVNPGEEDIVLKEDDVLKVDFGTHSNGRIM 140
Query: 178 DCAFTLTFNEKYDKLVEAVKDATNTGLREAGIDVRLCDIGEAIQETMESYEVELDGKTYK 357
D AFT+ F E L+ A ++ T TG+R GID R+CDIG I E + SYEVE++GKT+
Sbjct: 141 DSAFTVAFQENLQPLLMAAREGTETGIRSLGIDARVCDIGRDINEVITSYEVEIEGKTWP 200
Query: 358 VKPIRNLNGHSIGPYQIHAGKTVPIVKGGEATKMEENEYYAIETFGSTGKGFVRDDLECS 537
++P+ +L+GHSI ++IH G ++P V + T+++ + +YA+ETF +TGKGF+ D CS
Sbjct: 201 IRPVSDLHGHSISQFKIHGGISIPAVNNRDTTRIKGDTFYAVETFATTGKGFINDRSPCS 260
Query: 538 HYMKNFEIGHVPLRLARSKAL--LNTINKNFGTLAFCRRWLD--RLGEERYIGALKNLTE 705
H+M N H +L + + +FGTL F R LD L E + ++ LT
Sbjct: 261 HFMLNV---HKSRKLFNKDLIKVYEFVKSSFGTLPFSPRHLDHYNLVEGGSLKSVNLLTM 317
Query: 706 TGLVDPYPPLCDIKGCYTAQW-NIQYY*TNM*EVVSRGD 819
GL PYPPL DI G AQ+ + Y N E+++RGD
Sbjct: 318 MGLFTPYPPLNDIDGSKVAQFEHTVYLSENGKEILTRGD 356
>sp|Q6XMH6|AMPM2_ENCHE Methionine aminopeptidase 2 (MetAP 2) (Peptidase M 2) (MetAP2)
(MAP-2)
Length = 358
Score = 213 bits (543), Expect = 4e-55
Identities = 114/267 (42%), Positives = 164/267 (61%), Gaps = 7/267 (2%)
Frame = +1
Query: 40 GLAFPTGCSLNHVAAHYTPNAGDTTV-LKYDDVCKIDFGVHVNGRIIDCAFTLTFNEKYD 216
G+ FP G S+N AAHY+ N G+ + L +DV KIDFG H NGRI+D AFT+ F E+++
Sbjct: 94 GIGFPAGMSMNSCAAHYSVNPGEKDIILTENDVLKIDFGTHSNGRIMDSAFTIAFKEEFE 153
Query: 217 KLVEAVKDATNTGLREAGIDVRLCDIGEAIQETMESYEVELDGKTYKVKPIRNLNGHSIG 396
L+ A K+ T TG+R GID R+CDIG I E + SYE+E+DGK + ++P+ +L+GHSI
Sbjct: 154 PLLMAAKEGTETGIRSLGIDARVCDIGRDINEVISSYEMEVDGKKWAIRPVSDLHGHSIS 213
Query: 397 PYQIHAGKTVPIVKGGEATKMEENEYYAIETFGSTGKGFVRDDLECSHYMKNFEIGHVPL 576
++IH G ++P V + T++ + +YA+ETF +TG+GF+ D CSH+M N
Sbjct: 214 QFKIHGGISIPAVNNRDPTRITGDTFYAVETFATTGEGFINDRSPCSHFMINTH----KS 269
Query: 577 RLARSKALLNT---INKNFGTLAFCRRWLD--RLGEERYIGALKNLTETGLVDPYPPLCD 741
R +K L+ + +FGTL F R LD L E + ++ LT GL PYPPL D
Sbjct: 270 RKLYNKDLIKVYEFVRDSFGTLPFSPRHLDYYNLVEGSALKSVNLLTMMGLFTPYPPLND 329
Query: 742 IKGCYTAQWNIQYY*TNM-*EVVSRGD 819
I G AQ+ Y + E+++RGD
Sbjct: 330 IDGSKVAQFEHTVYLSESGKEILTRGD 356
>sp|Q8SR45|AMPM2_ENCCU Methionine aminopeptidase 2 (MetAP 2) (Peptidase M 2) (MetAP2)
(MAP-2)
Length = 358
Score = 212 bits (540), Expect = 9e-55
Identities = 122/287 (42%), Positives = 176/287 (61%), Gaps = 14/287 (4%)
Frame = +1
Query: 1 TSRTLIH-ERGLDAGLAFPTGCSLNHVAAHYTPNAGDTT-VLKYDDVCKIDFGVHVNGRI 174
++RTL+ ER + G+ FP G S+N AAHYT N G+ VLK DDV KIDFG H +GRI
Sbjct: 82 STRTLLKGER--NNGIGFPAGMSMNSCAAHYTVNPGEQDIVLKEDDVLKIDFGTHSDGRI 139
Query: 175 IDCAFTLTFNEKYDKLVEAVKDATNTGLREAGIDVRLCDIGEAIQETMESYEVELDGKTY 354
+D AFT+ F E + L+ A ++ T TG++ G+DVR+CDIG I E + SYEVE+ G+ +
Sbjct: 140 MDSAFTVAFKENLEPLLVAAREGTETGIKSLGVDVRVCDIGRDINEVISSYEVEIGGRMW 199
Query: 355 KVKPIRNLNGHSIGPYQIHAGKTVPIVKGGEATKMEENEYYAIETFGSTGKGFVRDDLEC 534
++PI +L+GHSI ++IH G ++P V + T+++ + +YA+ETF +TGKG + D C
Sbjct: 200 PIRPISDLHGHSISQFRIHGGISIPAVNNRDTTRIKGDSFYAVETFATTGKGSIDDRPPC 259
Query: 535 SHYMKNFEIGHVPLRLARSKALLNT--------INKNFGTLAFCRRWLDRLGEERYIGAL 690
SH++ L +S+ L N + + GTL F R LD G + G+L
Sbjct: 260 SHFV---------LNTYKSRKLFNKDLIKVYEFVKDSLGTLPFSPRHLDYYGLVKG-GSL 309
Query: 691 KN---LTETGLVDPYPPLCDIKGCYTAQW-NIQYY*TNM*EVVSRGD 819
K+ LT GL+ PYPPL DI GC AQ+ + Y + EV++RGD
Sbjct: 310 KSVNLLTMMGLLTPYPPLNDIDGCKVAQFEHTVYLSEHGKEVLTRGD 356
>sp|P50581|YD43_CAEEL Hypothetical protein T27A8.3 in chromosome X
Length = 182
Score = 161 bits (407), Expect = 2e-39
Identities = 79/123 (64%), Positives = 94/123 (76%), Gaps = 2/123 (1%)
Frame = +1
Query: 334 ELDGKTYKVKPIRNLNGHSIGPYQIHAGKTVPIVKGGEATKMEENEYYAIETFGSTGKGF 513
E G + +VKPI +LNG SI +IH GKTVPIVKGGE +MEENE YAIETFGSTGKG+
Sbjct: 29 EFHGNSNEVKPIGSLNGQSIAQCRIHTGKTVPIVKGGEQARMEENEIYAIETFGSTGKGY 88
Query: 514 VRDDLECSHYMKNFEIG--HVPLRLARSKALLNTINKNFGTLAFCRRWLDRLGEERYIGA 687
DD+E SHYMKNFE+ +PLRL +SK LL I+KNF TLAFCR W+DRL E +Y+ A
Sbjct: 89 FHDDMETSHYMKNFELADEKIPLRLQKSKGLLKLIDKNFATLAFCRCWIDRLEETKYLMA 148
Query: 688 LKN 696
LK+
Sbjct: 149 LKD 151
>sp|P56218|AMPM_PYRFU Methionine aminopeptidase (MAP) (Peptidase M)
Length = 295
Score = 150 bits (378), Expect = 5e-36
Identities = 93/243 (38%), Positives = 138/243 (56%), Gaps = 2/243 (0%)
Frame = +1
Query: 46 AFPTGCSLNHVAAHYTPNAGDTTVLKYDDVCKIDFGVHVNGRIIDCAFTLTFNEKYDKLV 225
AFP S+N +AAHYTP GDTTVLK D KID GVH++G I D A T+ + D+L+
Sbjct: 49 AFPVNLSINEIAAHYTPYKGDTTVLKEGDYLKIDVGVHIDGFIADTAVTVRVGMEEDELM 108
Query: 226 EAVKDATNTGLREAGIDVRLCDIGEAIQETMESYEVELDGKTYKVKPIRNLNGHSIGPYQ 405
EA K+A N + A V + ++G+AI+ E+ G KPI NL+GH I Y+
Sbjct: 109 EAAKEALNAAISVARAGVEIKELGKAIEN-----EIRKRG----FKPIVNLSGHKIERYK 159
Query: 406 IHAGKTVP-IVKGGEATKMEENEYYAIETFGSTGKGFVRDDLECSHYMKNFEIGHVPLRL 582
+HAG ++P I + + ++E + +AIE F + G G V +E + + VP+R+
Sbjct: 160 LHAGISIPNIYRPHDNYVLKEGDVFAIEPFATIGAGQV---IEVPPTLIYMYVRDVPVRV 216
Query: 583 ARSKALLNTINKNFGTLAFCRRWLDR-LGEERYIGALKNLTETGLVDPYPPLCDIKGCYT 759
A+++ LL I + +GTL F RWL + E + ALK L + G + YP L +I+
Sbjct: 217 AQARFLLAKIKREYGTLPFAYRWLQNDMPEGQLKLALKTLEKAGAIYGYPVLKEIRNGIV 276
Query: 760 AQW 768
AQ+
Sbjct: 277 AQF 279
>sp|O58362|AMPM_PYRHO Methionine aminopeptidase (MAP) (Peptidase M)
Length = 295
Score = 144 bits (364), Expect = 2e-34
Identities = 90/243 (37%), Positives = 136/243 (55%), Gaps = 2/243 (0%)
Frame = +1
Query: 46 AFPTGCSLNHVAAHYTPNAGDTTVLKYDDVCKIDFGVHVNGRIIDCAFTLTFNEKYDKLV 225
AFP SLN VAAHYTP GD TVLK D KID GVH++G I D A T+ +D+L+
Sbjct: 49 AFPANLSLNEVAAHYTPYKGDQTVLKEGDYLKIDLGVHIDGYIADTAVTVRVGMDFDELM 108
Query: 226 EAVKDATNTGLREAGIDVRLCDIGEAIQETMESYEVELDGKTYKVKPIRNLNGHSIGPYQ 405
EA K+A + + A V + ++G+AI+ E+ G PI NL+GH I Y+
Sbjct: 109 EAAKEALESAISVARAGVEVKELGKAIEN-----EIRKRG----FNPIVNLSGHKIERYK 159
Query: 406 IHAGKTVP-IVKGGEATKMEENEYYAIETFGSTGKGFVRDDLECSHYMKNFEIGHVPLRL 582
+HAG ++P I + + ++E + +AIE F +TG G V +E + + P+R+
Sbjct: 160 LHAGVSIPNIYRPHDNYVLQEGDVFAIEPFATTGAGQV---IEVPPTLIYMYVRDAPVRM 216
Query: 583 ARSKALLNTINKNFGTLAFCRRWLD-RLGEERYIGALKNLTETGLVDPYPPLCDIKGCYT 759
A+++ LL I + + TL F RWL + E + AL++L +G + YP L +I+G
Sbjct: 217 AQARFLLAKIKREYKTLPFAYRWLQGEMPEGQLKLALRSLERSGALYGYPVLREIRGGMV 276
Query: 760 AQW 768
Q+
Sbjct: 277 TQF 279
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 94,393,919
Number of Sequences: 369166
Number of extensions: 1925598
Number of successful extensions: 4762
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4591
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4722
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7859674995
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)