Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_003_M17
(889 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O08582|GTPB1_MOUSE GTP-binding protein 1 (G-protein 1) (... 162 1e-39
sp|O00178|GTPB1_HUMAN GTP-binding protein 1 (G-protein 1) (... 161 3e-39
sp|Q5R8Q7|GTPB1_PONPY GTP-binding protein 1 (G-protein 1) (... 161 3e-39
sp|Q18905|CGP1_CAEEL GTP-binding protein cgp-1 152 9e-37
sp|Q17045|AGP1_ASCSU GTP-binding protein AGP-1 147 3e-35
sp|P44323|IF2_HAEIN Translation initiation factor IF-2 44 5e-04
sp|Q9ZCZ8|IF2_RICPR Translation initiation factor IF-2 43 0.001
sp|P59587|IF2_ECOL6 Translation initiation factor IF-2 42 0.002
sp|Q8Z3H7|IF2_SALTI Translation initiation factor IF-2 42 0.002
sp|Q9ZF31|IF2_SALTY Translation initiation factor IF-2 42 0.002
>sp|O08582|GTPB1_MOUSE GTP-binding protein 1 (G-protein 1) (GP-1)
Length = 583
Score = 162 bits (410), Expect = 1e-39
Identities = 92/216 (42%), Positives = 127/216 (58%), Gaps = 2/216 (0%)
Frame = +1
Query: 166 NGKVTGLEPQELMESVMNLCKMAETLNASISVLRECHVGSRQALEILIRNVPTHFSTRET 345
+G GL ++ S + MAE + A + +LRE + + L+R E
Sbjct: 17 DGTEYGLSEADMEASYATVKSMAEQIEADVILLRERQEAGGRVRDYLVRKRVGDNDFLEV 76
Query: 346 RVAVLGNMDAGKSTLIGVLTQGVLDNGQGKTRLATFRHIHEVRSGRTSSISCQLLGFDKD 525
RVAV+GN+DAGKSTL+GVLT G LDNG+G R FRH HE+ SGRTSS+ +LGFD +
Sbjct: 77 RVAVVGNVDAGKSTLLGVLTHGELDNGRGFARQKLFRHKHEIESGRTSSVGNDILGFDSE 136
Query: 526 HNLFNFYWQKNRLHQRSHE--EVVLSSSKLVTLIDLAGYHKYQRTTLFALTARRPHLVLL 699
N+ N K H S E ++ SSK++T IDLAG+ KY +TT+F +T P +L
Sbjct: 137 GNVVN----KPDSHGGSLEWTKICEKSSKVITFIDLAGHEKYLKTTVFGMTGHLPDFCML 192
Query: 700 VIPANHGFKGTSIDHWHLAITFGVPIAVVVTKIDRC 807
++ +N G G + +H LA+ VP+ VVVTKID C
Sbjct: 193 MVGSNAGIVGMTKEHLGLALALNVPVFVVVTKIDMC 228
>sp|O00178|GTPB1_HUMAN GTP-binding protein 1 (G-protein 1) (GP-1) (GP1)
Length = 584
Score = 161 bits (407), Expect = 3e-39
Identities = 91/216 (42%), Positives = 127/216 (58%), Gaps = 2/216 (0%)
Frame = +1
Query: 166 NGKVTGLEPQELMESVMNLCKMAETLNASISVLRECHVGSRQALEILIRNVPTHFSTRET 345
+G GL ++ S + MAE + A + +LRE + + L+R E
Sbjct: 17 DGTEYGLSEADMEASYATVKSMAEQIEADVILLRERQEAGGRVRDYLVRKRVGDNDFLEV 76
Query: 346 RVAVLGNMDAGKSTLIGVLTQGVLDNGQGKTRLATFRHIHEVRSGRTSSISCQLLGFDKD 525
RVAV+GN+DAGKSTL+GVLT G LDNG+G R FRH HE+ SGRTSS+ +LGFD +
Sbjct: 77 RVAVVGNVDAGKSTLLGVLTHGELDNGRGFARQKLFRHKHEIESGRTSSVGNDILGFDSE 136
Query: 526 HNLFNFYWQKNRLHQRSHE--EVVLSSSKLVTLIDLAGYHKYQRTTLFALTARRPHLVLL 699
N+ N K H S E ++ S+K++T IDLAG+ KY +TT+F +T P +L
Sbjct: 137 GNVVN----KPDSHGGSLEWTKICEKSTKVITFIDLAGHEKYLKTTVFGMTGHLPDFCML 192
Query: 700 VIPANHGFKGTSIDHWHLAITFGVPIAVVVTKIDRC 807
++ +N G G + +H LA+ VP+ VVVTKID C
Sbjct: 193 MVGSNAGIVGMTKEHLGLALALNVPVFVVVTKIDMC 228
>sp|Q5R8Q7|GTPB1_PONPY GTP-binding protein 1 (G-protein 1) (GP-1) (GP1)
Length = 584
Score = 161 bits (407), Expect = 3e-39
Identities = 91/216 (42%), Positives = 127/216 (58%), Gaps = 2/216 (0%)
Frame = +1
Query: 166 NGKVTGLEPQELMESVMNLCKMAETLNASISVLRECHVGSRQALEILIRNVPTHFSTRET 345
+G GL ++ S + MAE + A + +LRE + + L+R E
Sbjct: 17 DGTEYGLSEADMEASYATVKSMAEQIEADVILLRERQEAGGRVRDYLVRKRVGDNDFLEV 76
Query: 346 RVAVLGNMDAGKSTLIGVLTQGVLDNGQGKTRLATFRHIHEVRSGRTSSISCQLLGFDKD 525
RVAV+GN+DAGKSTL+GVLT G LDNG+G R FRH HE+ SGRTSS+ +LGFD +
Sbjct: 77 RVAVVGNVDAGKSTLLGVLTHGELDNGRGFARQKLFRHKHEIESGRTSSVGNDILGFDSE 136
Query: 526 HNLFNFYWQKNRLHQRSHE--EVVLSSSKLVTLIDLAGYHKYQRTTLFALTARRPHLVLL 699
N+ N K H S E ++ S+K++T IDLAG+ KY +TT+F +T P +L
Sbjct: 137 GNVVN----KPDSHGGSLEWTKICEKSTKVITFIDLAGHEKYLKTTVFGMTGHLPDFCML 192
Query: 700 VIPANHGFKGTSIDHWHLAITFGVPIAVVVTKIDRC 807
++ +N G G + +H LA+ VP+ VVVTKID C
Sbjct: 193 MVGSNAGIVGMTKEHLGLALALNVPVFVVVTKIDMC 228
>sp|Q18905|CGP1_CAEEL GTP-binding protein cgp-1
Length = 613
Score = 152 bits (385), Expect = 9e-37
Identities = 93/215 (43%), Positives = 128/215 (59%), Gaps = 5/215 (2%)
Frame = +1
Query: 178 TGLEPQELMESVMNLCKMAETLNA-SISVLRECHVGSRQAL-EI-LIRNVPTHFSTRETR 348
+GL ++L ++ K+ E + A V+R G E+ +IR+ PT E R
Sbjct: 83 SGLSEEDLDKAADAQLKILEKIPAVGTKVIRRKQTGGGSLFTEVWIIRDPPTEKDFIEAR 142
Query: 349 VAVLGNMDAGKSTLIGVLTQGVLDNGQGKTRLATFRHIHEVRSGRTSSISCQLLGFDKDH 528
VAV+GN+DAGKSTL+GVLT LD+G+G R FRH HE SGRTSS+ +LGFD
Sbjct: 143 VAVVGNVDAGKSTLLGVLTHSALDDGRGAARTKLFRHKHEFESGRTSSVGNDILGFDVHG 202
Query: 529 NLFNFYWQKNRLHQRSHEEVVLSS--SKLVTLIDLAGYHKYQRTTLFALTARRPHLVLLV 702
N+ N K H + + V + S +KLVT IDLAG+ KY +TT+F +T P +L+
Sbjct: 203 NIVN----KPDPHNHNLDWVQIGSDCAKLVTFIDLAGHEKYLKTTIFGMTGHMPDYTMLM 258
Query: 703 IPANHGFKGTSIDHWHLAITFGVPIAVVVTKIDRC 807
I AN G GT+ +H LA++ VP+ +VVTKID C
Sbjct: 259 IGANMGIIGTTKEHLSLALSLHVPVYLVVTKIDMC 293
>sp|Q17045|AGP1_ASCSU GTP-binding protein AGP-1
Length = 591
Score = 147 bits (372), Expect = 3e-35
Identities = 92/224 (41%), Positives = 127/224 (56%), Gaps = 2/224 (0%)
Frame = +1
Query: 160 DDNGKVTGLEPQELMESVMNLCKMAETLNASISVLRECHVGSRQALEILIRNVPTHFSTR 339
DD K GL +EL + ++ N S S L G LIR+
Sbjct: 79 DDTQK--GLSKEELQIAEERHKQLMNQSNISSSHLVTKRCGDLYTSFHLIRDSVDADDFI 136
Query: 340 ETRVAVLGNMDAGKSTLIGVLTQGVLDNGQGKTRLATFRHIHEVRSGRTSSISCQLLGFD 519
E RVAV+GN+DAGKSTL+GVLT LD+G+G+ R FRH HE SGRTSS+ +LGF
Sbjct: 137 EVRVAVVGNVDAGKSTLLGVLTHSALDDGRGQARRKLFRHKHEFESGRTSSVGNDILGFS 196
Query: 520 KDHNLFNFYWQKNRLHQRSHEEVVL--SSSKLVTLIDLAGYHKYQRTTLFALTARRPHLV 693
D + N K +H + + V + S+K++T IDLAG+ KY +TT+F +T P
Sbjct: 197 MDGQIVN----KPDVHSGNLDWVSICRDSAKVITFIDLAGHEKYLKTTIFGMTGHAPDYT 252
Query: 694 LLVIPANHGFKGTSIDHWHLAITFGVPIAVVVTKIDRCQPDQAI 825
+L++ +N G G + +H LA++ VP+ VVVTKID C PDQ +
Sbjct: 253 MLMVGSNAGIIGMTKEHLSLALSLSVPVFVVVTKIDMC-PDQVL 295
>sp|P44323|IF2_HAEIN Translation initiation factor IF-2
Length = 829
Score = 44.3 bits (103), Expect = 5e-04
Identities = 51/206 (24%), Positives = 89/206 (43%), Gaps = 3/206 (1%)
Frame = +1
Query: 196 ELMESVMNLCKMAETLNASISVLRECHVGSRQALEILIRNVPTHFSTRETRVAVLGNMD- 372
E+++ +M + +M T+N I V ++++RN E AVLG+ D
Sbjct: 270 EIIKMMMKMGEMV-TINQVIDQETAQLVAEELGHKVILRN------ENELEEAVLGDRDV 322
Query: 373 -AGKSTLIGVLT-QGVLDNGQGKTRLATFRHIHEVRSGRTSSISCQLLGFDKDHNLFNFY 546
A K T V+T G +D+G KT L + +V +G I+ + +
Sbjct: 323 NAEKVTRAPVVTIMGHVDHG--KTSLLDYIRKAKVAAGEAGGITQHIGAY---------- 370
Query: 547 WQKNRLHQRSHEEVVLSSSKLVTLIDLAGYHKYQRTTLFALTARRPHLVLLVIPANHGFK 726
V + K++T +D G+ + T++ A A+ +V+LV+ A+ G
Sbjct: 371 ------------HVEMDDGKMITFLDTPGHAAF--TSMRARGAKATDIVVLVVAADDGVM 416
Query: 727 GTSIDHWHLAITFGVPIAVVVTKIDR 804
+I+ A G P+ V V KID+
Sbjct: 417 PQTIEAIQHAKAAGAPLVVAVNKIDK 442
>sp|Q9ZCZ8|IF2_RICPR Translation initiation factor IF-2
Length = 831
Score = 42.7 bits (99), Expect = 0.001
Identities = 26/83 (31%), Positives = 46/83 (55%)
Frame = +1
Query: 586 VVLSSSKLVTLIDLAGYHKYQRTTLFALTARRPHLVLLVIPANHGFKGTSIDHWHLAITF 765
V L+ SK +T ID G+ + + + A+ +V++V+ A+ G K +++ + A
Sbjct: 373 VTLADSKAITFIDTPGHEAFSE--MRSRGAKVTDIVIIVVAADDGIKTQTVEAINHAKAA 430
Query: 766 GVPIAVVVTKIDRCQPDQAINQL 834
GVPI V + KID+ PD I ++
Sbjct: 431 GVPIIVAINKIDK--PDIDIERI 451
>sp|P59587|IF2_ECOL6 Translation initiation factor IF-2
Length = 890
Score = 42.4 bits (98), Expect = 0.002
Identities = 51/218 (23%), Positives = 92/218 (42%), Gaps = 5/218 (2%)
Frame = +1
Query: 196 ELMESVMNLCKMAETLNASISVLRECHVGSRQALEILIRNVPTHFSTRETRVAVLGNMDA 375
++++++M L MA T+N I V ++++R E AV+ + D
Sbjct: 331 QVIKAMMKLGAMA-TINQVIDQETAQLVAEEMGHKVILRR------ENELEEAVMSDRDT 383
Query: 376 GKST---LIGVLTQGVLDNGQGKTRLATFRHIHEVRSGRTSSISCQLLGFDKDHNLFNFY 546
G + V G +D+G KT L + +V SG I+ + +
Sbjct: 384 GAAAEPRAPVVTIMGHVDHG--KTSLLDYIRSTKVASGEAGGITQHIGAYH--------- 432
Query: 547 WQKNRLHQRSHEEVVLSSSKLVTLIDLAGYHKYQRTTLFALTARRPHLVLLVIPANHGFK 726
V + + ++T +D G+ + T++ A A+ +V+LV+ A+ G
Sbjct: 433 --------------VETENGMITFLDTPGHAAF--TSMRARGAQATDIVVLVVAADDGVM 476
Query: 727 GTSIDHWHLAITFGVPIAVVVTKIDR--CQPDQAINQL 834
+I+ A GVP+ V V KID+ PD+ N+L
Sbjct: 477 PQTIEAIQHAKAAGVPVVVAVNKIDKPEADPDRVKNEL 514
>sp|Q8Z3H7|IF2_SALTI Translation initiation factor IF-2
Length = 892
Score = 42.4 bits (98), Expect = 0.002
Identities = 51/218 (23%), Positives = 92/218 (42%), Gaps = 5/218 (2%)
Frame = +1
Query: 196 ELMESVMNLCKMAETLNASISVLRECHVGSRQALEILIRNVPTHFSTRETRVAVLGNMDA 375
++++++M L MA T+N I V ++++R E AV+ + D
Sbjct: 333 QVIKAMMKLGAMA-TINQVIDQETAQLVAEEMGHKVILRR------ENELEEAVMSDRDT 385
Query: 376 GKST---LIGVLTQGVLDNGQGKTRLATFRHIHEVRSGRTSSISCQLLGFDKDHNLFNFY 546
G + V G +D+G KT L + +V SG I+ + +
Sbjct: 386 GAAAEPRAPVVTIMGHVDHG--KTSLLDYIRSTKVASGEAGGITQHIGAYH--------- 434
Query: 547 WQKNRLHQRSHEEVVLSSSKLVTLIDLAGYHKYQRTTLFALTARRPHLVLLVIPANHGFK 726
V + + ++T +D G+ + T++ A A+ +V+LV+ A+ G
Sbjct: 435 --------------VETDNGMITFLDTPGHAAF--TSMRARGAQATDIVVLVVAADDGVM 478
Query: 727 GTSIDHWHLAITFGVPIAVVVTKIDR--CQPDQAINQL 834
+I+ A GVP+ V V KID+ PD+ N+L
Sbjct: 479 PQTIEAIQHAKAAGVPVVVAVNKIDKPEADPDRVKNEL 516
>sp|Q9ZF31|IF2_SALTY Translation initiation factor IF-2
Length = 892
Score = 42.4 bits (98), Expect = 0.002
Identities = 51/218 (23%), Positives = 92/218 (42%), Gaps = 5/218 (2%)
Frame = +1
Query: 196 ELMESVMNLCKMAETLNASISVLRECHVGSRQALEILIRNVPTHFSTRETRVAVLGNMDA 375
++++++M L MA T+N I V ++++R E AV+ + D
Sbjct: 333 QVIKAMMKLGAMA-TINQVIDQETAQLVAEEMGHKVILRR------ENELEEAVMSDRDT 385
Query: 376 GKST---LIGVLTQGVLDNGQGKTRLATFRHIHEVRSGRTSSISCQLLGFDKDHNLFNFY 546
G + V G +D+G KT L + +V SG I+ + +
Sbjct: 386 GAAAEPRAPVVTIMGHVDHG--KTSLLDYIRSTKVASGEAGGITQHIGAYH--------- 434
Query: 547 WQKNRLHQRSHEEVVLSSSKLVTLIDLAGYHKYQRTTLFALTARRPHLVLLVIPANHGFK 726
V + + ++T +D G+ + T++ A A+ +V+LV+ A+ G
Sbjct: 435 --------------VETDNGMITFLDTPGHAAF--TSMRARGAQATDIVVLVVAADDGVM 478
Query: 727 GTSIDHWHLAITFGVPIAVVVTKIDR--CQPDQAINQL 834
+I+ A GVP+ V V KID+ PD+ N+L
Sbjct: 479 PQTIEAIQHAKAAGVPVVVAVNKIDKPEADPDRVKNEL 516
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 93,328,919
Number of Sequences: 369166
Number of extensions: 1775461
Number of successful extensions: 5541
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5277
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5523
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8886314050
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)