Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_003_L16
(705 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q13200|PSD2_HUMAN 26S proteasome non-ATPase regulatory s... 278 1e-74
sp|Q8VDM4|PSD2_MOUSE 26S proteasome non-ATPase regulatory s... 278 1e-74
sp|Q7S8R8|RPN1_NEUCR 26S proteasome regulatory subunit rpn-1 238 1e-62
sp|P87048|RPN1_SCHPO 26S proteasome regulatory subunit rpn1... 214 1e-55
sp|P38764|RPN1_YEAST 26S proteasome regulatory subunit RPN1... 191 2e-48
sp|Q6FPV6|RPN1_CANGA 26S proteasome regulatory subunit RPN1 188 1e-47
sp|O95704|ABB3_HUMAN Amyloid beta A4 precursor protein-bind... 30 6.2
sp|P55931|ETFD_PIG Electron transfer flavoprotein-ubiquinon... 30 8.2
>sp|Q13200|PSD2_HUMAN 26S proteasome non-ATPase regulatory subunit 2 (26S proteasome
regulatory subunit RPN1) (26S proteasome regulatory
subunit S2) (26S proteasome subunit p97) (Tumor necrosis
factor type 1 receptor associated protein 2) (55.11
protein)
Length = 908
Score = 278 bits (711), Expect = 1e-74
Identities = 137/197 (69%), Positives = 168/197 (85%)
Frame = +1
Query: 1 KFSHDSDQELAFTAILSMGLVGAGTNNARLAAMLRQLASFHSKDPHCLFAVRLAQGLLHL 180
KFSHD+D E+++ +I +MG+VG+GTNNARLAAMLRQLA +H+KDP+ LF VRLAQGL HL
Sbjct: 712 KFSHDADPEVSYNSIFAMGMVGSGTNNARLAAMLRQLAQYHAKDPNNLFMVRLAQGLTHL 771
Query: 181 GKGTLTISPHHQDRFLMSPVAVGGLLTVLISCLDVKRTFMNRNDYLLFYLTSAFQPRLLM 360
GKGTLT+ P+H DR LMS VAV GLLTVL+S LDV+ + ++ Y+L+ L +A QPR+L+
Sbjct: 772 GKGTLTLCPYHSDRQLMSQVAVAGLLTVLVSFLDVRNIILGKSHYVLYGLVAAMQPRMLV 831
Query: 361 TFDEALHPLTVTVRVGQGVDTVGQAGRPKTITGFQTHTTPVLLAAGERAELSTEEYLPVT 540
TFDE L PL V+VRVGQ VD VGQAG+PKTITGFQTHTTPVLLA GERAEL+TEE+LPVT
Sbjct: 832 TFDEELRPLPVSVRVGQAVDVVGQAGKPKTITGFQTHTTPVLLAHGERAELATEEFLPVT 891
Query: 541 QLPLEGFVILKKNPHYE 591
+ LEGFVIL+KNP+Y+
Sbjct: 892 PI-LEGFVILRKNPNYD 907
>sp|Q8VDM4|PSD2_MOUSE 26S proteasome non-ATPase regulatory subunit 2 (26S proteasome
regulatory subunit RPN1) (26S proteasome regulatory
subunit S2) (26S proteasome subunit p97)
Length = 908
Score = 278 bits (711), Expect = 1e-74
Identities = 137/197 (69%), Positives = 168/197 (85%)
Frame = +1
Query: 1 KFSHDSDQELAFTAILSMGLVGAGTNNARLAAMLRQLASFHSKDPHCLFAVRLAQGLLHL 180
KFSHD+D E+++ +I +MG+VG+GTNNARLAAMLRQLA +H+KDP+ LF VRLAQGL HL
Sbjct: 712 KFSHDADPEVSYNSIFAMGMVGSGTNNARLAAMLRQLAQYHAKDPNNLFMVRLAQGLTHL 771
Query: 181 GKGTLTISPHHQDRFLMSPVAVGGLLTVLISCLDVKRTFMNRNDYLLFYLTSAFQPRLLM 360
GKGTLT+ P+H DR LMS VAV GLLTVL+S LDV+ + ++ Y+L+ L +A QPR+L+
Sbjct: 772 GKGTLTLCPYHSDRQLMSQVAVAGLLTVLVSFLDVRNIILGKSHYVLYGLVAAMQPRMLV 831
Query: 361 TFDEALHPLTVTVRVGQGVDTVGQAGRPKTITGFQTHTTPVLLAAGERAELSTEEYLPVT 540
TFDE L PL V+VRVGQ VD VGQAG+PKTITGFQTHTTPVLLA GERAEL+TEE+LPVT
Sbjct: 832 TFDEELRPLPVSVRVGQAVDVVGQAGKPKTITGFQTHTTPVLLAHGERAELATEEFLPVT 891
Query: 541 QLPLEGFVILKKNPHYE 591
+ LEGFVIL+KNP+Y+
Sbjct: 892 PI-LEGFVILRKNPNYD 907
>sp|Q7S8R8|RPN1_NEUCR 26S proteasome regulatory subunit rpn-1
Length = 883
Score = 238 bits (607), Expect = 1e-62
Identities = 116/197 (58%), Positives = 154/197 (78%)
Frame = +1
Query: 1 KFSHDSDQELAFTAILSMGLVGAGTNNARLAAMLRQLASFHSKDPHCLFAVRLAQGLLHL 180
++SHD+D E+A AI +MGL+GAGTNNARLA +LRQLAS++ +D LF VR+AQGLLH+
Sbjct: 685 RYSHDNDNEVAINAIFAMGLLGAGTNNARLAQLLRQLASYYHRDQESLFMVRIAQGLLHM 744
Query: 181 GKGTLTISPHHQDRFLMSPVAVGGLLTVLISCLDVKRTFMNRNDYLLFYLTSAFQPRLLM 360
GKGTL++SP H DR ++S VA GLL VL++ +D K+ +++ YLL+++ +A PR+L+
Sbjct: 745 GKGTLSVSPFHTDRQVLSNVATAGLLAVLVAMIDAKQFITSKSHYLLYWIVTAMHPRMLV 804
Query: 361 TFDEALHPLTVTVRVGQGVDTVGQAGRPKTITGFQTHTTPVLLAAGERAELSTEEYLPVT 540
T DE L PLTV VRVGQ VD VGQAGRPKTITG+QT +TPVLL GERAEL ++Y+ ++
Sbjct: 805 TLDEDLKPLTVNVRVGQAVDVVGQAGRPKTITGWQTQSTPVLLGYGERAELEDDQYISLS 864
Query: 541 QLPLEGFVILKKNPHYE 591
LEG VIL+KNP +E
Sbjct: 865 S-TLEGLVILRKNPDWE 880
>sp|P87048|RPN1_SCHPO 26S proteasome regulatory subunit rpn1 (Proteasome non-ATPase subunit
mts4) (19S regulatory cap region of 26S protease subunit
2)
Length = 891
Score = 214 bits (546), Expect = 1e-55
Identities = 108/193 (55%), Positives = 147/193 (76%)
Frame = +1
Query: 1 KFSHDSDQELAFTAILSMGLVGAGTNNARLAAMLRQLASFHSKDPHCLFAVRLAQGLLHL 180
++SHD+D ++A+ AI +MGLVGAGT+NARLA +LRQLAS++ K+ + LF VR+AQGLL+L
Sbjct: 693 RYSHDNDLDVAYNAIFAMGLVGAGTSNARLAQLLRQLASYYHKESNALFMVRIAQGLLYL 752
Query: 181 GKGTLTISPHHQDRFLMSPVAVGGLLTVLISCLDVKRTFMNRNDYLLFYLTSAFQPRLLM 360
GKGT+T++P+H +R ++ A GL+TV+++ LD ++ + +LL+ +T A +PR+L+
Sbjct: 753 GKGTMTLNPYHTERQILGQTAFAGLMTVVLAMLDANTFVLDTSHWLLYAITLAIRPRMLI 812
Query: 361 TFDEALHPLTVTVRVGQGVDTVGQAGRPKTITGFQTHTTPVLLAAGERAELSTEEYLPVT 540
T E L V+VRVGQ VD VGQAGRPK ITG+ THTTPVLL ERAEL+TE Y P+T
Sbjct: 813 TLGEDGQYLPVSVRVGQAVDVVGQAGRPKVITGWVTHTTPVLLHHNERAELATEAYTPLT 872
Query: 541 QLPLEGFVILKKN 579
LEG VILKKN
Sbjct: 873 S--LEGIVILKKN 883
>sp|P38764|RPN1_YEAST 26S proteasome regulatory subunit RPN1 (Proteasome non-ATPase subunit
1)
Length = 993
Score = 191 bits (485), Expect = 2e-48
Identities = 100/198 (50%), Positives = 138/198 (69%)
Frame = +1
Query: 1 KFSHDSDQELAFTAILSMGLVGAGTNNARLAAMLRQLASFHSKDPHCLFAVRLAQGLLHL 180
+FSHD+D E++ +I +MGL GAGTNNARLA +LRQLAS++S++ LF RLAQGLLHL
Sbjct: 798 RFSHDADLEVSMNSIFAMGLCGAGTNNARLAQLLRQLASYYSREQDALFITRLAQGLLHL 857
Query: 181 GKGTLTISPHHQDRFLMSPVAVGGLLTVLISCLDVKRTFMNRNDYLLFYLTSAFQPRLLM 360
GKGT+T+ + D +++ V + +LT + V +FM ++ L + L + +P+ ++
Sbjct: 858 GKGTMTMDVFN-DAHVLNKVTLASILTTAVGL--VSPSFMLKHHQLFYMLNAGIRPKFIL 914
Query: 361 TFDEALHPLTVTVRVGQGVDTVGQAGRPKTITGFQTHTTPVLLAAGERAELSTEEYLPVT 540
++ P+ V VRVGQ V+TVGQAGRPK ITG+ T +TPVLL GERAEL T+EY+ T
Sbjct: 915 ALNDEGEPIKVNVRVGQAVETVGQAGRPKKITGWITQSTPVLLNHGERAELETDEYISYT 974
Query: 541 QLPLEGFVILKKNPHYEE 594
+EG VILKKNP Y E
Sbjct: 975 S-HIEGVVILKKNPDYRE 991
>sp|Q6FPV6|RPN1_CANGA 26S proteasome regulatory subunit RPN1
Length = 983
Score = 188 bits (478), Expect = 1e-47
Identities = 97/198 (48%), Positives = 140/198 (70%)
Frame = +1
Query: 1 KFSHDSDQELAFTAILSMGLVGAGTNNARLAAMLRQLASFHSKDPHCLFAVRLAQGLLHL 180
+FSHD D +++ +I +MGL G GTNNARLA +LRQLAS++S++ LF RLAQGL+HL
Sbjct: 788 RFSHDPDLDVSMNSIFAMGLCGVGTNNARLAQLLRQLASYYSREQDALFITRLAQGLVHL 847
Query: 181 GKGTLTISPHHQDRFLMSPVAVGGLLTVLISCLDVKRTFMNRNDYLLFYLTSAFQPRLLM 360
GKGT+T+ + D +++ V + LLTVL+ + +F+ ++ L + L S +P+ ++
Sbjct: 848 GKGTMTMDIFN-DAHVLNKVTLASLLTVLVGL--ISPSFILKHHQLFYMLNSGVRPKFII 904
Query: 361 TFDEALHPLTVTVRVGQGVDTVGQAGRPKTITGFQTHTTPVLLAAGERAELSTEEYLPVT 540
T +E + V VR+GQ V+TVGQAG+PKTITG+ T +TPVLL GERAEL +EY+ T
Sbjct: 905 TLNEEGEQIKVNVRIGQAVETVGQAGKPKTITGWITQSTPVLLGHGERAELENDEYISYT 964
Query: 541 QLPLEGFVILKKNPHYEE 594
+EG VILKKNP+++E
Sbjct: 965 N-NIEGVVILKKNPNFQE 981
>sp|O95704|ABB3_HUMAN Amyloid beta A4 precursor protein-binding family B member 3
(Fe65-like protein 2) (Fe65L2)
Length = 486
Score = 30.0 bits (66), Expect = 6.2
Identities = 25/82 (30%), Positives = 40/82 (48%), Gaps = 10/82 (12%)
Frame = +1
Query: 370 EALHPLTVTVRVGQGVDTVGQAGRPKTITGFQTHTTPVLLAAGE--------RAELSTEE 525
EAL+ T+ V G+D + +A T G + P +L+ + +AE STEE
Sbjct: 292 EALYMGTLPVTKAMGMDVLNEAIGTLTARGDRNAWVPTMLSVSDSLMTAHPIQAEASTEE 351
Query: 526 YLPVTQLP--LEGFVILKKNPH 585
P+ Q P L F+ + ++PH
Sbjct: 352 E-PLWQCPVRLVTFIGVGRDPH 372
>sp|P55931|ETFD_PIG Electron transfer flavoprotein-ubiquinone oxidoreductase,
mitochondrial precursor (ETF-QO) (ETF-ubiquinone
oxidoreductase) (ETF dehydrogenase)
(Electron-transferring-flavoprotein dehydrogenase)
Length = 607
Score = 29.6 bits (65), Expect = 8.2
Identities = 18/47 (38%), Positives = 24/47 (51%)
Frame = +1
Query: 58 LVGAGTNNARLAAMLRQLASFHSKDPHCLFAVRLAQGLLHLGKGTLT 198
+VGAG A L+QLA+ H KD V L + H+G TL+
Sbjct: 63 IVGAGPAGLSAATRLKQLAAQHEKD----LRVCLVEKAAHIGAHTLS 105
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 79,751,641
Number of Sequences: 369166
Number of extensions: 1576981
Number of successful extensions: 3641
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 3549
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3633
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6170718545
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)